pBBR1MCS2-pAmp-EGFP
$128.00
In stock
SKU
PL82690
100μg liquid plasmid(Instantly):$207
| name | pBBR1MCS2-pAmp-EGFP | another name | apBBR1MCS2-pAmp-EGFP |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | pAMP | Replicon | pBBR1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13R： | |
| 3′ sequencing primer | M13F： | Copy number | Extremely low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACCTTCGGGA GCGCCTGAAG CCCGTTCTGG ACGCCCTGGG GCCGTTGAAT CGGGATATGC AGGCCAAGGC CGCCGCGATC ATCAAGGCCG TGGGCGAAAA GCTGCTGACG GAACAGCGGG AAGTCCAGCG CCAGAAACAG GCCCAGCGCC AGCAGGAACG CGGGCGCGCA CATTTCCCCG AAAAGTGCCA CCTGGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG CGATAGCTAG ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC AAGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT GGCGATTCAG GTTCATCATG CCGTTTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA ATTACAACAG TTTTTATGCA TGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG CAATTAACCC TCACTAAAGG GAACAAAAGC TGGGTACCGG GCCCCCCCTC GAGGTCGACC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG CTCAAGCTTC GAATTCTGCA GTCGACGGTA CCGCGGGCCC GGGATCCACC GGATCTAGAT AATCTAGAGC GGCCGCCACC GCGGTGGAGC TCCAATTCGC CCTATAGTGA GTCGTATTAC GCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGACTGCGA TGAGTGGCAG GGCGGGGCGT AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TGCTACGCCT GAATAAGTGA TAATAAGCGG ATGAATGGCA GAAATTCGAA AGCAAATTCG ACCCGGTCGT CGGTTCAGGG CAGGGTCGTT AAATAGCCGC TTATGTCTAT TGCTGGTTTA CCGGTTTATT GACTACCGGA AGCAGTGTGA CCGTGTGCTT CTCAAATGCC TGAGGCCAGT TTGCTCAGGC TCTCCCCGTG GAGGTAATAA TTGACGATAT GATCATTTAT TCTGCCTCCC AGAGCCTGAT AAAAACGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GAGGCGGCTA CAGCCGATAG TCTGGAACAG CGCACTTACG GGTTGCTGCG CAACCCAAGT GCTACCGGCG CGGCAGCGTG ACCCGTGTCG GCGGCTCCAA CGGCTCGCCA TCGTCCAGAA AACACGGCTC ATCGGGCATC GGCAGGCGCT GCTGCCCGCG CCGTTCCCAT TCCTCCGTTT CGGTCAAGGC TGGCAGGTCT GGTTCCATGC CCGGAATGCC GGGCTGGCTG GGCGGCTCCT CGCCGGGGCC GGTCGGTAGT TGCTGCTCGC CCGGATACAG GGTCGGGATG CGGCGCAGGT CGCCATGCCC CAACAGCGAT TCGTCCTGGT CGTCGTGATC AACCACCACG GCGGCACTGA ACACCGACAG GCGCAACTGG TCGCGGGGCT GGCCCCACGC CACGCGGTCA TTGACCACGT AGGCCGACAC GGTGCCGGGG CCGTTGAGCT TCACGACGGA GATCCAGCGC TCGGCCACCA AGTCCTTGAC TGCGTATTGG ACCGTCCGCA AAGAACGTCC GATGAGCTTG GAAAGTGTCT TCTGGCTGAC CACCACGGCG TTCTGGTGGC CCATCTGCGC CACGAGGTGA TGCAGCAGCA TTGCCGCCGT GGGTTTCCTC GCAATAAGCC CGGCCCACGC CTCATGCGCT TTGCGTTCCG TTTGCACCCA GTGACCGGGC TTGTTCTTGG CTTGAATGCC GATTTCTCTG GACTGCGTGG CCATGCTTAT CTCCATGCGG TAGGGTGCCG CACGGTTGCG GCACCATGCG CAATCAGCTG CAACTTTTCG GCAGCGCGAC AACAATTATG CGTTGCGTAA AAGTGGCAGT CAATTACAGA TTTTCTTTAA CCTACGCAAT GAGCTATTGC GGGGGGTGCC GCAATGAGCT GTTGCGTACC CCCCTTTTTT AAGTTGTTGA TTTTTAAGTC TTTCGCATTT CGCCCTATAT CTAGTTCTTT GGTGCCCAAA GAAGGGCACC CCTGCGGGGT TCCCCCACGC CTTCGGCGCG GCTCCCCCTC CGGCAAAAAG TGGCCCCTCC GGGGCTTGTT GATCGACTGC GCGGCCTTCG GCCTTGCCCA AGGTGGCGCT GCCCCCTTGG AACCCCCGCA CTCGCCGCCG TGAGGCTCGG GGGGCAGGCG GGCGGGCTTC GCCTTCGACT GCCCCCACTC GCATAGGCTT GGGTCGTTCC AGGCGCGTCA AGGCCAAGCC GCTGCGCGGT CGCTGCGCGA GCCTTGACCC GCCTTCCACT TGGTGTCCAA CCGGCAAGCG AAGCGCGCAG GCCGCAGGCC GGAGGCTTTT CCCCAGAGAA AATTAAAAAA ATTGATGGGG CAAGGCCGCA GGCCGCGCAG TTGGAGCCGG TGGGTATGTG GTCGAAGGCT GGGTAGCCGG TGGGCAATCC CTGTGGTCAA GCTCGTGGGC AGGCGCAGCC TGTCCATCAG CTTGTCCAGC AGGGTTGTCC ACGGGCCGAG CGAAGCGAGC CAGCCGGTGG CCGCTCGCGG CCATCGTCCA CATATCCACG GGCTGGCAAG GGAGCGCAGC GACCGCGCAG GGCGAAGCCC GGAGAGCAAG CCCGTAGGGC GCCGCAGCCG CCGTAGGCGG TCACGACTTT GCGAAGCAAA GTCTAGTGAG TATACTCAAG CATTGAGTGG CCCGCCGGAG GCACCGCCTT GCGCTGCCCC CGTCGAGCCG GTTGGACACC AAAAGGGAGG GGCAGGCATG GCGGCATACG CGATCATGCG ATGCAAGAAG CTGGCGAAAA TGGGCAACGT GGCGGCCAGT CTCAAGCACG CCTACCGCGA GCGCGAGACG CCCAACGCTG ACGCCAGCAG GACGCCAGAG AACGAGCACT GGGCGGCCAG CAGCACCGAT GAAGCGATGG GCCGACTGCG CGAGTTGCTG CCAGAGAAGC GGCGCAAGGA CGCTGTGTTG GCGGTCGAGT ACGTCATGAC GGCCAGCCCG GAATGGTGGA AGTCGGCCAG CCAAGAACAG CAGGCGGCGT TCTTCGAGAA GGCGCACAAG TGGCTGGCGG ACAAGTACGG GGCGGATCGC ATCGTGACGG CCAGCATCCA CCGTGACGAA ACCAGCCCGC ACATGACCGC GTTCGTGGTG CCGCTGACGC AGGACGGCAG GCTGTCGGCC AAGGAGTTCA TCGGCAACAA AGCGCAGATG ACCCGCGACC AGACCACGTT TGCGGCCGCT GTGGCCGATC TAGGGCTGCA ACGGGGCATC GAGGGCAGCA AGGCACGTCA CACGCGCATT CAGGCGTTCT ACGAGGCCCT GGAGCGGCCA CCAGTGGGCC ACGTCACCAT CAGCCCGCAA GCGGTCGAGC CACGCGCCTA TGCACCGCAG GGATTGGCCG AAAAGCTGGG AATCTCAAAG CGCGTTGAGA CGCCGGAAGC CGTGGCCGAC CGGCTGACAA AAGCGGTTCG GCAGGGGTAT GAGCCTGCCC TACAGGCCGC CGCAGGAGCG CGTGAGATGC GCAAGAAGGC CGATCAAGCC CAAGAGACGG CCCGAG
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