pAX01-PPK
$142.00
In stock
SKU
PL82687
整合型木糖诱导
100μg liquid plasmid(Instantly):$163
| name | pAX01-PPK | another name | apAX01-PPK |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Escherichia coli | Prokaryotic resistance | Amp |
| Selective marker | Ery | Fluorescent labeling | |
| Promoter | xylA | Replicon | repB |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | rrnBT1-R：gagcgttcaccgacaaacaac | |
| 3′ sequencing primer | pAX01-R：tttatccaccgaactaagttgg | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGGGTGATGT CAAAGCTTGA AAAAACGCAC GTAACAAAAG CAAAATTTAT GCTCCATGGG GGAGACTACA ACCCCGATCA GTGGCTGGAT CGGCCCGATA TTTTAGCTGA CGATATCAAA CTGATGAAGC TTTCTCATAC GAATACGTTT TCTGTCGGCA TTTTTGCATG GAGCGCACTT GAGCCGGAGG AGGGCGTATA TCAATTTGAA TGGCTGGATG ATATTTTTGA GCGGATTCAC AGTATAGGCG GCCGGGTCAT ATTAGCAACG CCGAGCGGAG CCCGTCCGGC CTGGCTGTCG CAAACCTATC CGGAAGTTTT GCGCGTCAAT GCCTCCCGCG TCAAACAGCT GCACGGCGGA AGGCACAACC ACTGCCTCAC ATCTAAAGTC TACCGAGAAA AAACACGGCA CATCAACCGC TTATTAGCAG AACGATACGG ACATCACCCG GCGCTGTTAA TGTGGCACAT TTCAAACGAA TACGGGGGAG ATTGCCACTG TGAATCGATG CGGCCGCTCT AGAGCAACGT TCTTGCCATT GCTGCATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA TTAATTAATC ATCGGGAAGA TCTTCATCAC CGAAACGCGG CAGGCAGCTC TAGAGTTAAC AAGAGTTTGT AGAAACGCAA AAAGGCCATC CGTCAGGATG GCCTTCTGCT TAGCTAGAGC GGCGGATTTG TCCTACTCAG GAGAGCGTTC ACCGACAAAC AACAGATAAA ACGAAAGGCC CAGTCTTTCG ACTGAGCCTT TCGTTTTATT TGATGCCTCA AGCTAGAGAG TCATTACCAG ATCTCACTGC AGAGATCCCC GGGTCTAGAG TCTAGGGACC TCTTTAGCTC CTTGGAAGCT GTCAGTAGTA TACCTAATAA TTTATCTACA TTCCCTTTAG TAACGTGTAA CTTTCCAAAT TTACAAAAGC GACTCATAGA ATTATTTCCT CCCGTTAAAT AATAGATAAC TATTAAAAAT AGACAATACT TGCTCATAAG TAACGGTACT TAAATTGTTT ACTTTGGCGT GTTTCATTGC TTGATGAAAC TGATTTTTAG TAAACAGTTG ACGATATTCT CGATTGACCC ATTTTGAAAC AAAGTACGTA TATAGCTTCC AATATTTATC TGGAACATCT GTGGTATGGC GGGTAAGTTT TATTAAGACA CTGTTTACTT TTGGTTTAGG ATGAAAGCAT TCCGCTGGCA GCTTAAGCAA TTGCTGAATC GAGACTTGAG TGTGCAAGAG CAACCCTAGT GTTCGGTGAA TATCCAAGGT ACGCTTGTAG AATCCTTCTT CAACAATCAG ATAGATGTCA GACGCATGGC TTTCAAAAAC CACTTTTTTA ATAATTTGTG TGCTTAAATG GTAAGGAATA CTCCCAACAA TTTTATACCT CTGTTTGTTA GGGAATTGAA ACTGTAGAAT ATCTTGGTGA ATTAAAGTGA CACGAGTATT CAGTTTTAAT TTTTCTGACG ATAAGTTGAA TAGATGACTG TCTAATTCAA TAGACGTTAC CTGTTTACTT ATTTTAGCCA GTTTCGTCGT TAAATGCCCT TTACCTGTTC CAATTTCGTA AACGGTATCG GTTTCTTTTA AATTCAATTG TTTTATTATT TGGTTGAGTA CTTTTTCACT CGTTAAAAAG TTTTGAGAAT ATTTTATATT TTTGTTCATG TAATCACTCC TTCTTAATTA CAAATTTTTA GCATCTAATT TAACTTCAAT TCCTATTATA CAAAATTTTA AGATACTGCA CTATCAACAC ACTCTTAAGT TTGCTTCTAA GTCTTATTTC CATAACTTCT TTTACGTTTC CGCCATTCTT TGCTGTTTCG ATTTTTATGA TATGGTGCAA GTCAGCACGA ACACGAACCG TCTTATCTCC CATTATATCT TTTTTTGCAC TGATTGGTGT ATCATTTCGT TTTTCTTTTG TGCTAGAGGA TCAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTGCGGCCGC CCGCGGTTAA TGATGGTGGT GATGATGTTC AGGTTGTTCA AGAGATTTGA TGTAATCGTA GATAGCAAGT TGAGCACGAA CTTTACGACG GTTGCCACGA GGAACGTAAC GGTTAGAAAG TTCTTTATCG ATGTAACGAG CTTTAACTGT ATCAGAGAAA AGGATATCGA TGATATCAAG AACACGTTGT TTAAGACGAG GATCAAGAAG AGGTGTAGCA ACTTCGATAC GGTAATCGAT GTTACGTGTC ATCCAATCAG CAGAAGAAAG GTAAACTTTT TTATCGCCGC CGTTTTCGAA GATGTAAACA CGATCATGTT CAAGGTAACG ATCAACGATA GAGATAGCAC GGATGTTATC AGAGATGCCT TCAAGGTTAG GGATAAGAGA GCACATGCCA CGAACAAGAA GGTTAACAGG AACGCCAGAA GAAGAAGCAG CGTAAAGACG ATCAACAAGG CCTTTATCAA CAAGGTTGTT AAGTTTAAGT GTGATGCCAG AAGGAAGGCC TTGTTGAGCG TTAGCGATTT CACGATCAAC CATTTCGTAA AGAAGACGAC GAGAGTTTTG AGGAGAAACC ATAAGGTAAT CGAATGTAAC AGGACGGTAA GGGTTTTCGA TGAAGTTGAA AACACGACGA ACTTCGTTTG TGATACGAGC ATCAGCTGTA AGAAGAGAGT AATCTGTGTA AAGACGAGCT GTTTTTTCGT TGAAGTTGCC TGTGCCGATA TGAGCGTAAC GAACAACTTC GCCGTTTTCT TTACGAGAGA TAAGGAAAAG TTTAGCATGG ATTTTAAGGC CAGGAGCAGA GAAGATAACA TGAACGCCAG CTTCTGTAAG ACGTTTAGCC CAATGGATGT TAGCTTCTTC ATCGAAACGA GCTTGAAGTT CAACAACAAC TGTAACTTTT TTGCCGTTAT GAGCAGCATG GATCATAGAA TCGATGATAC GAGAATCTTT AGCAACACGG TAGATGTTGA TTTTGATAGC AAGAACAGAA GGATCGAAAG AAGCTTGACG AAGAAGTTCA AGAACATGTT CGAATGTATG GTAAGGGTAG TAAAGAAGAA CATCACGTTC ACGGATAGCA TCGAAGCCGT TACGGAATTG AGCTTTATCG AACCAGATAT GACGAAGACG AGGAAGAGGT TTGTTAACAA GGTTAGCTTT GCCAACGTTA GGGAAGTTGA TGAAATCTTT GAAGTTATGG TAACGGCCGC CAGGAACGAT AGAATCGTAA CGAGAGATTG TAAGTTTTTC ACGAAGAACT TCAACAAGAG CGTTAGGCAT ATCACGTTGG TAAACGAAAC GAACAGGTTC AGCTGTAAGA CGTTGTTTAA GAGAAGAAGA CATAAGTTCC ATAAGAGAAG CTTCCATTTC ATGAACAAGA TCGTATTCAG CATCACGTGT CATTTTCATA GAGTAAGCGT TAAGAGCATC GTAATCGAAG AAGCCTTTGA AGATATCATC AAGGCAGTAA CGAAGGATGT TATCAAGAAG GATCATAGGT TTACGACGAC GAGGAGCTTC AGGAGGAAGG TTAACGAAAC GAGGAACTTT ATCAGAAGGG ATTTCAAGAA GAGCGTAACG GATTGTATCG CCACGGATGA TTTCAACAGC AAGGTATGTG TAATCATCTT TAAGGAATTG AACAAGATCT GTATCAGGGT TGATAAGGAT AGGTGTGATA TGTTGACGAA GGTATTGTTT GAAGTAATGA CGAAGCCAGT TTTGTTGGTT AACAGAAAGT TGACGTTCGT TGATAAGGAA GATTTGGTTA CGAGCCATTT CAAGAAGAAG TTCGTTGTAA AGGCCATCGA ATTCTTGATC AGCTTTAAGA ACACGAGATT GGATTTTGCC AAGAAGATGA CGAGAATGAG AGTTAGAGCC TTGTTCTTCA GAGATGATGA TACGACGTTT AAGTTCAGCG AAACGAACTT TGTAGAATTC ATCAAGGTTG TTAGAGTAGA TGCCAAGGAA ACGCATACGT TCGATAAGAG GGTTAGATTT ATCAGCAGCT TCTTGAAGAA CACGTTCGTT GAAAGAAAGC CAAGAAAGTT CTTTTTCGAT GTAAAGTTTT TCTTGGCCCA TGGATCCCAT TTCCCCCTTT GATTTTTAGA TATCACTAGT TTGGACCATT TGTCATTTCC CCCTTTGATT TAAGTGAACA AGTTTATCCA TCAACTATCT TAATTGAGTT AGTTTGTTTA TCCAATAAAC TAACTTTATC TCATCATATA CAAAATAAAT GTTTATTTCA ATGTTTTTTT TAGAAAATTT AGTTATAATA TTAGATATGA TACTTTTAAA TATCTAATTC AAGCTTCAAA AAACACCAAC TTAGTTCGGT GGATAAACAA AGGAGTGGTT ATTATTCAAA TTGCAGATCA AGCTTTAGTA AAAAAAATGA ATCAAAAATT AATATTAGAT GAAATTTTGA AGAACTCCCC TGTCTCCAGG GCAACTCTCT CTGAGATTAC AGGATTAAAC AAGTCTACTG TCTCCTCTCA AGTAAATACA CTGCTTGAAA AAGATTTTAT TTTTGAAATT GGGGCAGGGC AATCTAGAGG CGGCAGAAGA CCTGTAATGC TTGTTTTTAA TAAGAATGCA GGCTACTCGA TTGGTATTGA TATAGGAGTC GACTATCTTA ACGGAATTCT AACCGACTTA GAAGGAAATA TTATTCTCGA GAAGACTTCT GACTTGTCTA GTTCTTCCGC TAGTGAAGTA AAAGAGATTT TATTTGCACT TATTCATGGT TTTGTAACCC ATATGCCTGA GTCCCCTTAT GGTCTAGTCG GAATAGGAAT TTGTGTTCCA GGCCTTGTAG ATCGTCATCA GCAAATTATT TTCATGCCTA ACTTAAATTG GAATATCAAA GATTTGCAGT TTTTAATTGA GAGTGAGTTT AATGTTCCGG TTTTTGTTGA AAATGAAGCT AATGCAGGAG CATACGGTGA AAAAGTATTT GGTATGACAA AAAACTATGA AAACATCGTT TACATCAGTA TTAATATCGG AATTGGAACT GGACTTGTTA TTAACAACGA ATTGTATAAA GGTGTTCAGG GTTTTTCTGG GGAAATGGGT CATATGACGA TAGATTTTAA TGGACCCAAA TGCAGCTGTG GAAATCGAGG CTGTTGGGAA TTATATGCTT CTGAAAAAGC GTTACTGGCT TCGCTCTCTA AAGAAGAAAA GAATATTTCT CGAAAAGAGA TTGTGGAACG CGCAAATAAA AATGATGTAG AAATGTTAAA TGCACTTCAA AACTTTGGCT TTTATATCGG AATTGGATTA ACCAATATCC TTAATACATT TGATATAGAA GCTGTTATCT TGAGAAATCA TATAATTGAA TCTCATCCCA TTGTTTTAAA TACGATTAAA AACGAAGTTT CTTCTAGAGT CCATTCTCAT TTAGACAATA AATGTGAACT ATTGCCTTCT TCGTTAGGAA AAAATGCACC TGCTTTAGGA GCGGTTTCTA TCGTTATTGA TTCTTTTTTA AGTGTTACCC CTATAAGTTA GGAGCTCCCC GGGACGTTCT TGCCATTGCT GCATAAAAAA CGCCCGGCGG CAACCGAGCG TTCTGAATTA ATTAATCATC GCGACTGCAG AGATATCGAT TTCAAGCTAT ATTTGGAGTT GAGCCTCTTG AAACGGACAC CCTGTATCCG AAGGATCGAA ACGCTGTCAG CTACCGCAGC CAAATATATG AAATGAAGGA TTATGCAACC GTGATTGATG TAAAGACAGC TTCAGTGGAA GCGGTGTATC AAGAAGATTT TTATGCGCGC ACGCCAGCGG TCACAAGCCA TGAGTATCAG CAGGGCAAGG CGTATTTTAT CGGCGCGCGT TTGGAGGATC AATTTCAGCG TGATTTCTAT GAGGGTCTGA TCACAGACCT GTCTCTCTCT CCAGTTTTTC CGGTTCGGCA CGGAAAAGGC GTCTCCGTAC AAGCGAGGCA GGATCAGGAC AATGATTATA TTTTTGTCAT GAATTTCACG GAAGAAAAAC AGCTGGTCAC GTTTGATCAG AGTGTGAAGG ACATAATGAC AGGAGACATA TTGTCAGGCG ACCTGACGAT GGAAAAGTAT GAAGTGAGAA TTGTCGTAAA CACACATTAG CCCATCATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTTGATG CCTCCGTGTA AGGGGGAATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAC TAAGAAAATG CCGTCAAATC CGCTCGCCAT GACTTCACTA ACGATGCCTT TGAAAATCTT CAAGTTCTTT TCTACTAATT CAAGGCGTGT CTCACCAGGT TTTTGGTTTG CTCCGGCGCA AATGCAGACA ATATCAGGAT
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