pCDNA3-FLAG-DHX36
$113.00
In stock
SKU
PL82660
G4R1;RHAU;DDX36;MLEL1
100μg liquid plasmid(Instantly):$134
| name | pCDNA3-FLAG-DHX36 | another name | apCDNA3-FLAG-DHX36 |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTACCATG GATTACAAGG ATGACGACGA TAAGGGATCC AGTTATGACT ACCATCAGAA CTGGGGCCGT GATGGGGGTC CCCGCAGCTC CGGTGGGGGC TATGGAGGGG GGCCAGCAGG GGGTCATGGA GGTAACCGAG GCTCCGGAGG AGGCGGCGGC GGCGGAGGGG GTGGTCGAGG CGGCAGGGGC CGGCATCCCG GGCACCTGAA AGGCCGCGAA ATCGGCATGT GGTACGCGAA AAAACAGGGG CAGAAGAACA AGGAAGCGGA GAGGCAAGAG AGAGCTGTAG TACACATGGA TGAACGACGA GAAGAACAAA TTGTACAGTT ACTGAATTCT GTTCAAGCGA AGAATGATAA AGAGTCAGAA GCACAGATAT CCTGGTTTGC TCCTGAGGAT CATGGATACG GTACTGAAGT TTCTACTAAG AACACACCAT GCTCAGAGAA CAAACTTGAC ATCCAGGAAA AGAAGTTGAT AAATCAAGAA AAAAAAATGT TTAGAATCAG GAACAGATCA TATATTGACC GAGATTCTGA GTATCTCTTG CAAGAAAATG AACCAGATGG AACTTTAGAC CAAAAATTAT TGGAAGATTT ACAAAAGAAA AAAAATGACC TTCGGTATAT TGAAATGCAG CATTTCAGAG AAAAGCTGCC TTCGTATGGA ATGCAAAAGG AATTGGTAAA TTTAATTGAT AACCATCAGG TAACAGTAAT AAGTGGTGAA ACTGGTTGTG GCAAAACCAC TCAAGTTACT CAGTTCATTT TGGATAACTA CATTGAAAGA GGAAAAGGAT CTGCTTGCAG AATAGTTTGT ACTCAGCCAA GAAGAATTAG TGCCATTTCA GTTGCGGAAA GAGTAGCTGC AGAAAGGGCA GAATCTTGTG GCAGTGGTAA TAGTACTGGA TATCAAATTC GTCTCCAGAG TCGGTTGCCA AGGAAACAGG GTTCTATCTT ATACTGTACA ACAGGAATCA TCCTTCAGTG GCTCCAGTCA GACCCGTATT TGTCCAGTGT TAGTCATATC GTACTTGATG AAATCCATGA AAGAAATCTG CAGTCAGATG TTTTAATGAC TGTTGTTAAA GACCTTCTCA ATTTTCGATC TGACTTGAAA GTAATATTGA TGAGTGCAAC ATTGAATGCA GAAAAGTTTT CAGAATATTT TGGTAACTGT CCAATGATAC ATATACCTGG TTTTACCTTT CCGGTTGTGG AATATCTTTT GGAAGATGTA ATTGAAAAAA TAAGGTATGT TCCAGAACAA AAAGAACACA GATGCCAGTT TAAGAGGGGT TTCATGCAAG GGCATGTAAA TAGACAAGAA AAAGAAGAAA AAGAAGCAAT ATATAAAGAA CGTTGGCCAG ATTATGTAAG GGAACTGCGA AGAAGGTATT CTGCAAGTAC TGTAGATGTT ATAGAAATGA TGGAGGATGA TAAAGTTGAT CTGAATTTGA TTGTTGCCCT CATCCGATAC ATTGTTTTGG AAGAAGAGGA TGGTGCGATA CTGGTCTTTC TGCCAGGCTG GGACAATATC AGCACTTTAC ATGATCTCTT GATGTCACAA GTAATGTTTA AATCAGATAA ATTTTTAATT ATACCTTTAC ATTCACTGAT GCCTACAGTT AACCAGACAC AGGTGTTTAA AAGAACCCCT CCTGGTGTTC GGAAAATAGT AATTGCTACC AACATTGCGG AGACTAGCAT TACCATAGAT GATGTCGTTT ATGTGATAGA TGGAGGAAAA ATAAAAGAGA CGCATTTTGA TACTCAGAAC AATAACAGTA CAATGTCCGC TGAGTGGGTT AGTAAAGCTA ATGCCAAACA GAGAAAAGGT CGAGCTGGAA GAGTTCAACC TGGTCATTGC TATCATCTGT ATAATGGTCT TAGAGCAAGT CTTCTAGATG ACTATCAACT GCCAGAAATT TTGAGAACTC CTTTGGAAGA ACTTTGTTTA CAAATAAAGA TTTTAAGGCT AGGTGGAATT GCTTATTTTC TGAGTAGATT AATGGACCCA CCATCAAATG AGGCAGTGTT ACTCTCCATA AGACACCTGA TGGAGCTGAA CGCTTTGGAT AAACAAGAAG AATTGACACC TCTTGGAGTC CACTTGGCAC GATTACCCGT TGAGCCACAT ATTGGAAAAA TGATTCTTTT TGGAGCACTG TTCTGCTGCT TAGACCCAGT ACTCACTATT GCTGCTAGTC TCAGTTTCAA AGATCCATTT GTCATTCCAC TGGGCTGGGA AGAGGCTAGG CGACGTGGTT TCAGATACGA AAAGGACTAT TGCTGGGAAT ATTTTCTGTC TTCAAACACA CTGCAGATGC TGCATAACAT GAAAGGACAG TTTGCTGAGC ATCTTCTTGG AGCTGGATTT GTAAGCAGTA GAAATCCTAA AGATCCAGAA TCTAATATAA ATTCAGATAA TGAGAAGATA ATTAAAGCTG TCATCTGTGC TGGTTTATAT CCCAAAGTTG CTAAAATTCG ACTAAATTTG GGTAAAAAAA GAAAAATGGT AAAAGTTTAC ACAAAAACCG ATGGCCTGGT TGCTGTTCAT CCTAAATCTG TTAATGTGGA GCAAACAGAC TTTCACTACA ACTGGCTTAT CTATCACCTA AAGATGAGAA CAAGCAGTAT ATACTTGTAT GACTGCACAG AGGTTTCCCC ATACTGTCTC TTGTTTTTTG GAGGTGACAT TTCCATCCAG AAGGATAACG ATCAGGAAAC TATTGCTGTA GATGAGTGGA TTGTATTTCA GTCTCCAGCA AGAATTGCCC ATCTTGTTAA GGAATTAAGA AAGGAACTAG ATATTCTTCT GCAAGAGAAG ATTGAAAGTC CTCATCCTGT AGACTGGAAT GACACTAAAT CCAGAGACTG TGCAGTACTG TCAGCTATTA TAGACTTGAT CAAAACACAG GAAAAGGCAA CTCCCAGGAA CTTTCCGCCA CGATTCCAGG ATGGATATTA CAGCTGACTC GAGCATGCAT CTAGAGGGCC CTATTCTATA GTGTCACCTA AATGCTAGAG CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGCATCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGG GGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTC
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