pPSdown
$99.00
In stock
SKU
PL82555
100μg liquid plasmid(Instantly):$120
| name | pPSdown | another name | apPSdown |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Signal report | Fragment type | Promoter |
| Fragment species | Empty vector | Prokaryotic resistance | Spe |
| Selective marker | Bar | Fluorescent labeling | Green |
| Promoter | Replicon | pSa | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTAAAACGAC GGCCAGTGAG CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG GGCCCCCCCT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCCGC GGCCGCAGGA GCTGCCGTCT TCAGTGCCTG CAGGCATAGA AGACCCGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCTTCAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCACG GCATGGACGA GCTGTACAAG TAAAACTCCG AGACCACAAG GCGCGCCTAG TGGTCTCCAG GAAAATTGCC CAGAATTAAT TCCCCAACTG CACAGAACAA ATTTCCCTTT GCTTTTGTGT AGACCTCAAA ACTTTATCCC CCATCTTTGA TCTTATCCCT TGTTTTTCTG CTTTTTTCTT CTTTCTTGGG TTTTGATTTC CGGACTTAAC GTTTGTTTTC CGGTTTGCGA GACATATTCT ATCGGATTCT CAACTGTCTG ATGAAATAAA TATGTAATGT TCTATAAGTC TTTCAATTTG ATGGGATGTT CCACTTCTGT ATAGGAGCCA ATTGATTGAC AACATGCATC AATCGGAGCT CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGAA GGCCTTGACA GGATATATTG GCGGGTAAAC TAAGTCGCTG TATGTGTTTG TTTGAGATCT CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAGA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG TGTAACATTG GTCTAGTGAT TATTTGCCGA CTACCTTGGT GATCTCGCCT TTCACGTAGT GAACAAATTC TTCCAACTGA TCTGCGCGCG AGGCCAAGCG ATCTTCTTGT CCAAGATAAG CCTGCCTAGC TTCAAGTATG ACGGGCTGAT ACTGGGCCGG CAGGCGCTCC ATTGCCCAGT CGGCAGCGAC ATCCTTCGGC GCGATTTTGC CGGTTACTGC GCTGTACCAA ATGCGGGACA ACGTAAGCAC TACATTTCGC TCATCGCCAG CCCAGTCGGG CGGCGAGTTC CATAGCGTTA AGGTTCCATT TAGCGCCTCA AATAGATCCT GTTCAGGAAC CGGATCAAAG AGTTCCTCCG CCGCTGGACC TACCAAGGCA ACGCTATGTT CTCTTGCTTT TGTCAGCAAG ATAGCCAGAT CAATGTCGAT CGTGGCTGGC TCGAAGATAC CTGCAAGAAT GTCATTGCGC TGCCATTCTC CAAATTGCAG TTCGCGCTTA GCTGGATAAC GCCACGGAAT GATGTCGTCG TGCACAACAA TGGTGACTTC TACAGCGCGG AGAATCTCGC TCTCTCCAGG GGAAGCCGAA GTTTCCAAAA GGTCGTTGAT CAAAGCTCGC CGCGTTGTTT CATCAAGCCT TACGGTCACC GTAACCAGCA AATCAATATC ACTGTGTGGC TTCAGGCCGC CATCCACTGC GGAGCCGTAC AAATGTACGG CCAGCAACGT CGGTTCGAGA TGGCGCTCGA TGACGCCAAC TACCTCTGAT AGTTGAGTCG ATACTTCGGC GATCACCGCT TCCCTCATAA CACCCCTTGT ATTACTGTTT ATGTAAGCAG ACAGTTTTAT TGTTCATGAT GATATATTTT TATCTTGTGC AATGTAACAT CAGAGATTTT GAGACACAAC GTGGCTTTGT TGAATAAATC GAACTTTTGC TGAGTTGAAG GATCAGATCA CGCATCTTCC CGACAACGCA GACCGTTCCG TGGCAAAGCA AAAGTTCAAA ATCACCAACT GGTCCACCTA CAACAAAGCT CTCATCAACC GTGGCTCCCT CACTTTCTGG CTGGATGATG GGGCGATTCA GGCGATCCCC ATCCAACAGC CCGCCGTCGA GCGGGCTTTT TTATCCCCGG AAGCCTGTGG ATAGAGGGTA GTTATCCACG TGAAACCGCT AATGCCCCGC AAAGCCTTGA TTCACGGGGC TTTCCGGCCC GCTCCAAAAA CTATCCACGT GAAATCGCTA ATCAGGGTAC GTGAAATCGC TAATCGGAGT ACGTGAAATC GCTAATAAGG TCACGTGAAA TCGCTAATCA AAAAGGCACG TGAGAACGCT AATAGCCCTT TCAGATCAAC AGCTTGCAAA CACCCCTCGC TCCGGCAAGT AGTTACAGCA AGTAGTATGT TCAATTAGCT TTTCAATTAT GAATATATAT ATCAATTATT GGTCGCCCTT GGCTTGTGGA CAATGCGCTA CGCGCACCGG CTCCGCCCGT GGACAACCGC AAGCGGTTGC CCACCGTCGA GCGCCTTTGC CCACAACCCG GCGGCCGGCC GCAACAGATC GTTTTATAAA TTTTTTTTTT TTGAAAAAGA AAAAGCCCGA AAGGCGGCAA CCTCTCGGGC TTCTGGATTT CCGATCCCCG GAATTAGATC TTGGCAGGAT ATATTGTGGT GTAACGTTAT CAGCTTGCAT GCCGGTCGAT CTAGTAACAT AGATGACACC GCGCGCGATA ATTTATCCTA GTTTGCGCGC TATATTTTGT TTTCTATCGC GTATTAAATG TATAATTGCG GGACTCTAAT CATAAAAACC CATCTCATAA ATAACGTCAT GCATTACATG TTAATTATTA CATGCTTAAC GTAATTCAAC AGAAATTATA TGATAATCAT CGCAAGACCG GCAACAGGAT TCAATCTTAA GAAACTTTAT TGCCAAATGT TTTAACGATC TGCTTGACTC TAGGGGTCAT CAGATTTCGG TGACGGGCAG GACCGGACGG GGCGGCACCG GCAGGCTGAA GTCCAGCTGC CAGAAACCCA CGTCATGCCA GTTCCCGTGC TTGAAGCCGG CCGCCCGCAG CATGCCGCGG GGGGCATATC CGAGCGCCTC GTGCATGCGC ACGCTCGGGT CGTTGGGCAG CCCGATGACA GCGACCACGC TCTTGAAGCC CTGTGCCTCC AGGGACTTCA GCAGGTGGGT GTAGAGCGTG GAGCCCAGTC CCGTCCGCTG GTGGCGGGGG GAGACGTACA CGGTCGACTC GGCCGTCCAG TCGTAGGCGT TGCGTGCCTT CCAGGGACCC GCGTAGGCGA TGCCGGCGAC CTCGCCGTCC ACCTCGGCGA CGAGCCAGGG ATAGCGCTCC CGCAGACGGA CGAGGTCGTC CGTCCACTCC TGCGGTTCCT GCGGCTCGGT ACGGAAGTTG ACCGTGCTTG TCTCGATGTA GTGGTTGACG ATGGTGCAGA CCGCCGGCAT GTCCGCCTCG GTGGCACGGC GGATGTCGGC CGGGCGTCGT TCTGGGCTCA TGGTAGATCC CCTCGATCGA GTTGAGAGTG AATATGAGAC TCTAATTGGA TACCGAGGGG AATTTATGGA ACGTCAGTGG AGCATTTTTG ACAAGAAATA TTTGCTAGCT GATAGTGACC TTAGGCGACT TTTGAACGCG CAATAATGGT TTCTGACGTA TGTGCTTAGC TCATTAAACT CCAGAAACCC GCGGCTCAGT GGCTCCTTCA ACGTTGCGGT TCTGTCAGTT CCAAACGTAA AACGGCTTGT CCCGCGTCAT CGGCGGGGGT CATAACGTGA CTCCCTTAAT TCTCCGCTCA TGATCGATAA CATTAACGCT TACAATTTCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
