pAdEasy-1
$99.00
In stock
SKU
PL82498
100μg liquid plasmid(Instantly):$134
| name | pAdEasy-1 | another name | apAdEasy-1 |
| Insert Size (bp) | Plasmid host | Mammalian cell,Adenovirus | |
| Plasmid uses | packaging aid | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | M13F： | |
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC GCCGGCATGG CGGCCGACGC GCTGGGCTAC GTCTTGCTGG CGTTCGCGAC GCGAGGCTGG ATGGCCTTCC CCATTATGAT TCTTCTCGCT TCCGGCGGCA TCGGGATGCC CGCGTTGCAG GCCATGCTGT CCAGGCAGGT AGATGACGAC CATCAGGGAC AGCTTCAAGG ATCGCTCGCG GCTCTTACCA GCCTAACTTC GATCATTGGA CCGCTGATCG TCACGGCGAT TTATGCCGCC TCGGCGAGCA CATGGAACGG GTTGGCATGG ATTGTAGGCG CCGCCCTATA CCTTGTCTGC CTCCCCGCGT TGCGTCGCGG TGCATGGAGC CGGGCCACCT CGACCTGAAT GGAAGCCGGC GGCACCTCGC TAACGGATTC ACCACTCCAA GAATTGGAGC CAATCAATTC TTGCGGAGAA CTGTGAATGC GCAAACCAAC CCTTGGCAGA ACATATCCAT CGCGTCCGCC ATCTCCAGCA GCCGCACGCG GCGCATCTCG GGCAGCGTTG GGTCCTGGCC ACGGGTGCGC ATGATCGTGC TCCTGTCGTT GAGGACCCGG CTAGGCTGGC GGGGTTGCCT TACTGGTTAG CAGAATGAAT CACCGATACG CGAGCGAACG TGAAGCGACT GCTGCTGCAA AACGTCTGCG ACCTGAGCAA CAACATGAAT GGTCTTCGGT TTCCGTGTTT CGTAAAGTCT GGAAACGCGG AAGTCAGCGC CCTGCACCAT TATGTTCCGG ATCTGCATCG CAGGATGCTG CTGGCTACCC TGTGGAACAC CTACATCTGT ATTAACGAAG CGCTGGCATT GACCCTGAGT GATTTTTCTC TGGTCCCGCC GCATCCATAC CGCCAGTTGT TTACCCTCAC AACGTTCCAG TAACCGGGCA TGTTCATCAT CAGTAACCCG TATCGTGAGC ATCCTCTCTC GTTTCATCGG TATCATTACC CCCATGAACA GAAATCCCCC TTACACGGAG GCATCAGTGA CCAAACAGGA AAAAACCGCC CTTAACATGG CCCGCTTTAT CAGAAGCCAG ACATTAACGC TTCTGGAGAA ACTCAACGAG CTGGACGCGG ATGAACAGGC AGACATCTGT GAATCGCTTC ACGACCACGC TGATGAGCTT TACCGCAGCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA CCCAGTCACG TAGCGATAGC GGAGTGTATA CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT GCAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ACACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGTCTA GCTACGAATT CTTCGACAGC TTCGAAACTA GAATCGATTT CGAAACTAGC TTAAGGGTGG GAAAGAATAT ATAAGGTGGG GGTCTTATGT AGTTTTGTAT CTGTTTTGCA GCAGCCGCCG CCGCCATGAG CACCAACTCG TTTGATGGAA GCATTGTGAG CTCATATTTG ACAACGCGCA TGCCCCCATG GGCCGGGGTG CGTCAGAATG TGATGGGCTC CAGCATTGAT GGTCGCCCCG TCCTGCCCGC AAACTCTACT ACCTTGACCT ACGAGACCGT GTCTGGAACG CCGTTGGAGA CTGCAGCCTC CGCCGCCGCT TCAGCCGCTG CAGCCACCGC CCGCGGGATT GTGACTGACT TTGCTTTCCT GAGCCCGCTT GCAAGCAGTG CAGCTTCCCG TTCATCCGCC CGCGATGACA AGTTGACGGC TCTTTTGGCA CAATTGGATT CTTTGACCCG GGAACTTAAT GTCGTTTCTC AGCAGCTGTT GGATCTGCGC CAGCAGGTTT CTGCCCTGAA GGCTTCCTCC CCTCCCAATG CGGTTTAAAA CATAAATAAA AAACCAGACT CTGTTTGGAT TTGGATCAAG CAAGTGTCTT GCTGTCTTTA TTTAGGGGTT TTGCGCGCGC GGTAGGCCCG GGACCAGCGG TCTCGGTCGT TGAGGGTCCT GTGTATTTTT TCCAGGACGT GGTAAAGGTG ACTCTGGATG TTCAGATACA TGGGCATAAG CCCGTCTCTG GGGTGGAGGT AGCACCACTG CAGAGCTTCA TGCTGCGGGG TGGTGTTGTA GATGATCCAG TCGTAGCAGG AGCGCTGGGC GTGGTGCCTA AAAATGTCTT TCAGTAGCAA GCTGATTGCC AGGGGCAGGC CCTTGGTGTA AGTGTTTACA AAGCGGTTAA GCTGGGATGG GTGCATACGT GGGGATATGA GATGCATCTT GGACTGTATT TTTAGGTTGG CTATGTTCCC AGCCATATCC CTCCGGGGAT TCATGTTGTG CAGAACCACC AGCACAGTGT ATCCGGTGCA CTTGGGAAAT TTGTCATGTA GCTTAGAAGG AAATGCGTGG AAGAACTTGG AGACGCCCTT GTGACCTCCA AGATTTTCCA TGCATTCGTC CATAATGATG GCAATGGGCC CACGGGCGGC GGCCTGGGCG AAGATATTTC TGGGATCACT AACGTCATAG TTGTGTTCCA GGATGAGATC GTCATAGGCC ATTTTTACAA AGCGCGGGCG GAGGGTGCCA GACTGCGGTA TAATGGTTCC ATCCGGCCCA GGGGCGTAGT TACCCTCACA GATTTGCATT TCCCACGCTT TGAGTTCAGA TGGGGGGATC ATGTCTACCT GCGGGGCGAT GAAGAAAACG GTTTCCGGGG TAGGGGAGAT CAGCTGGGAA GAAAGCAGGT TCCTGAGCAG CTGCGACTTA CCGCAGCCGG TGGGCCCGTA AATCACACCT ATTACCGGCT GCAACTGGTA GTTAAGAGAG CTGCAGCTGC CGTCATCCCT GAGCAGGGGG GCCACTTCGT TAAGCATGTC CCTGACTCGC ATGTTTTCCC TGACCAAATC CGCCAGAAGG CGCTCGCCGC CCAGCGATAG CAGTTCTTGC AAGGAAGCAA AGTTTTTCAA CGGTTTGAGA CCGTCCGCCG TAGGCATGCT TTTGAGCGTT TGACCAAGCA GTTCCAGGCG GTCCCACAGC TCGGTCACCT GCTCTACGGC ATCTCGATCC AGCATATCTC CTCGTTTCGC GGGTTGGGGC GGCTTTCGCT GTACGGCAGT AGTCGGTGCT CGTCCAGACG GGCCAGGGTC ATGTCTTTCC ACGGGCGCAG GGTCCTCGTC AGCGTAGTCT GGGTCACGGT GAAGGGGTGC GCTCCGGGCT GCGCGCTGGC CAGGGTGCGC TTGAGGCTGG TCCTGCTGGT GCTGAAGCGC TGCCGGTCTT CGCCCTGCGC GTCGGCCAGG TAGCATTTGA CCATGGTGTC ATAGTCCAGC CCCTCCGCGG CGTGGCCCTT GGCGCGCAGC TTGCCCTTGG AGGAGGCGCC GCACGAGGGG CAGTGCAGAC TTTTGAGGGC GTAGAGCTTG GGCGCGAGAA ATACCGATTC CGGGGAGTAG GCATCCGCGC CGCAGGCCCC GCAGACGGTC TCGCATTCCA CGAGCCAGGT GAGCTCTGGC CGTTCGGGGT CAAAAACCAG GTTTCCCCCA TGCTTTTTGA TGCGTTTCTT ACCTCTGGTT TCCATGAGCC GGTGTCCACG CTCGGTGACG AAAAGGCTGT CCGTGTCCCC GTATACAGAC TTGAGAGGCC TGTCCTCGAG CGGTGTTCCG CGGTCCTCCT CGTATAGAAA CTCGGACCAC TCTGAGACAA AGGCTCGCGT CCAGGCCAGC ACGAAGGAGG CTAAGTGGGA GGGGTAGCGG TCGTTGTCCA CTAGGGGGTC CACTCGCTCC AGGGTGTGAA GACACATGTC GCCCTCTTCG GCATCAAGGA AGGTGATTGG TTTGTAGGTG TAGGCCACGT GACCGGGTGT TCCTGAAGGG GGGCTATAAA AGGGGGTGGG GGCGCGTTCG TCCTCACTCT CTTCCGCATC GCTGTCTGCG AGGGCCAGCT GTTGGGGTGA GTACTCCCTC TGAAAAGCGG GCATGACTTC TGCGCTAAGA TTGTCAGTTT CCAAAAACGA GGAGGATTTG ATATTCACCT GGCCCGCGGT GATGCCTTTG AGGGTGGCCG CATCCATCTG GTCAGAAAAG ACAATCTTTT TGTTGTCAAG CTTGGTGGCA AACGACCCGT AGAGGGCGTT GGACAGCAAC TTGGCGATGG AGCGCAGGGT TTGGTTTTTG TCGCGATCGG CGCGCTCCTT GGCCGCGATG TTTAGCTGCA CGTATTCGCG CGCAACGCAC CGCCATTCGG GAAAGACGGT GGTGCGCTCG TCGGGCACCA GGTGCACGCG CCAACCGCGG TTGTGCAGGG TGACAAGGTC AACGCTGGTG GCTACCTCTC CGCGTAGGCG CTCGTTGGTC CAGCAGAGGC GGCCGCCCTT GCGCGAGCAG AATGGCGGTA GGGGGTCTAG CTGCGTCTCG TCCGGGGGGT CTGCGTCCAC GGTAAAGACC CCGGGCAGCA GGCGCGCGTC GAAGTAGTCT ATCTTGCATC CTTGCAAGTC TAGCGCCTGC TGCCATGCGC GGGCGGCAAG CGCGCGCTCG TATGGGTTGA GTGGGGGACC CCATGGCATG GGGTGGGTGA GCGCGGAGGC GTACATGCCG CAAATGTCGT AAACGTAGAG GGGCTCTCTG AGTATTCCAA GATATGTAGG GTAGCATCTT CCACCGCGGA TGCTGGCGCG CACGTAATCG TATAGTTCGT GCGAGGGAGC GAGGAGGTCG GGACCGAGGT TGCTACGGGC GGGCTGCTCT GCTCGGAAGA CTATCTGCCT GAAGATGGCA TGTGAGTTGG ATGATATGGT TGGACGCTGG AAGACGTTGA AGCTGGCGTC TGTGAGACCT ACCGCGTCAC GCACGAAGGA GGCGTAGGAG TCGCGCAGCT TGTTGACCAG CTCGGCGGTG ACCTGCACGT CTAGGGCGCA GTAGTCCAGG GTTTCCTTGA TGATGTCATA CTTATCCTGT CCCTTTTTTT TCCACAGCTC GCGGTTGAGG ACAAACTCTT CGCGGTCTTT CCAGTACTCT TGGATCGGAA ACCCGTCGGC CTCCGAACGG TAAGAGCCTA GCATGTAGAA CTGGTTGACG GCCTGGTAGG CGCAGCATCC CTTTTCTACG GGTAGCGCGT ATGCCTGCGC GGCCTTCCGG AGCGAGGTGT GGGTGAGCGC AAAGGTGTCC CTGACCATGA CTTTGAGGTA CTGGTATTTG AAGTCAGTGT CGTCGCATCC GCCCTGCTCC CAGAGCAAAA AGTCCGTGCG CTTTTTGGAA CGCGGATTTG GCAGGGCGAA GGTGACATCG TTGAAGAGTA TCTTTCCCGC GCGAGGCATA AAGTTGCGTG TGATGCGGAA GGGTCCCGGC ACCTCGGAAC GGTTGTTAAT TACCTGGGCG GCGAGCACGA TCTCGTCAAA GCCGTTGATG TTGTGGCCCA CAATGTAAAG TTCCAAGAAG CGCGGGATGC CCTTGATGGA AGGCAATTTT TTAAGTTCCT CGTAGGTGAG CTCTTCAGGG GAGCTGAGCC CGTGCTCTGA AAGGGCCCAG TCTGCAAGAT GAGGGTTGGA AGCGACGAAT GAGCTCCACA GGTCACGGGC CATTAGCATT TGCAGGTGGT CGCGAAAGGT CCTAAACTGG CGACCTATGG CCATTTTTTC TGGGGTGATG CAGTAGAAGG TAAGCGGGTC TTGTTCCCAG CGGTCCCATC CAAGGTTCGC GGCTAGGTCT CGCGCGGCAG TCACTAGAGG CTCATCTCCG CCGAACTTCA TGACCAGCAT GAAGGGCACG AGCTGCTTCC CAAAGGCCCC CATCCAAGTA TAGGTCTCTA CATCGTAGGT GACAAAGAGA CGCTCGGTGC GAGGATGCGA GCCGATCGGG AAGAACTGGA TCTCCCGCCA CCAATTGGAG GAGTGGCTAT TGATGTGGTG AAAGTAGAAG TCCCTGCGAC GGGCCGAACA CTCGTGCTGG CTTTTGTAAA AACGTGCGCA GTACTGGCAG CGGTGCACGG GCTGTACATC CTGCACGAGG TTGACCTGAC GACCGCGCAC AAGGAAGCAG AGTGGGAATT TGAGCCCCTC GCCTGGCGGG TTTGGCTGGT GGTCTTCTAC TTCGGCTGCT TGTCCTTGAC CGTCTGGCTG CTCGAGGGGA GTTACGGTGG ATCGGACCAC CACGCCGCGC GAGCCCAAAG TCCAGATGTC CGCGCGCGGC GGTCGGAGCT TGATGACAAC ATCGCGCAGA TGGGAGCTGT CCATGGTCTG GAGCTCCCGC GGCGTCAGGT CAGGCGGGAG CTCCTGCAGG TTTACCTCGC ATAGACGGGT CAGGGCGCGG GCTAGATCCA GGTGATACCT AATTTCCAGG GGCTGGTTGG TGGCGGCGTC GATGGCTTGC AAGAGGCCGC ATCCCCGCGG CGCGACTACG GTACCGCGCG GCGGGCGGTG GGCCGCGGGG GTGTCCTTGG ATGATGCATC TAAAAGCGGT GACGCGGGCG AGCCCCCGGA GGTAGGGGGG GCTCCGGACC CGCCGGGAGA GGGGGCAGGG GCACGTCGGC GCCGCGCGCG GGCAGGAGCT GGTGCTGCGC GCGTAGGTTG CTGGCGAACG CGACGACGCG GCGGTTGATC TCCTGAATCT GGCGCCTCTG CGTGAAGACG ACGGGCCCGG TGAGCTTGAA CCTGAAAGAG AGTTCGACAG AATCAATTTC GGTGTCGTTG ACGGCGGCCT GGCGCAAAAT CTCCTGCACG TCTCCTGAGT TGTCTTGATA GGCGATCTCG GCCATGAACT GCTCGATCTC TTCCTCCTGG AGATCTCCGC GTCCGGCTCG CTCCACGGTG GCGGCGAGGT CGTTGGAAAT GCGGGCCATG AGCTGCGAGA AGGCGTTGAG GCCTCCCTCG TTCCAGACGC GGCTGTAGAC CACGCCCCCT TCGGCATCGC GGGCGCGCAT GACCACCTGC GCGAGATTGA GCTCCACGTG CCGGGCGAAG ACGGCGTAGT TTCGCAGGCG CTGAAAGAGG TAGTTGAGGG TGGTGGCGGT GTGTTCTGCC ACGAAGAAGT ACATAACCCA GCGTCGCAAC GTGGATTCGT TGATATCCCC CAAGGCCTCA AGGCGCTCCA TGGCCTCGTA GAAGTCCACG GCGAAGTTGA AAAACTGGGA GTTGCGCGCC GACACGGTTA ACTCCTCCTC CAGAAGACGG ATGAGCTCGG CGACAGTGTC GCGCACCTCG CGCTCAAAGG CTACAGGGGC CTCTTCTTCT TCTTCAATCT CCTCTTCCAT AAGGGCCTCC CCTTCTTCTT CTTCTGGCGG CGGTGGGGGA GGGGGGACAC GGCGGCGACG ACGGCGCACC GGGAGGCGGT CGACAAAGCG CTCGATCATC TCCCCGCGGC GACGGCGCAT GGTCTCGGTG ACGGCGCGGC CGTTCTCGCG GGGGCGCAGT TGGAAGACGC CGCCCGTCAT GTCCCGGTTA TGGGTTGGCG GGGGGCTGCC ATGCGGCAGG GATACGGCGC TAACGATGCA TCTCAACAAT TGTTGTGTAG GTACTCCGCC GCCGAGGGAC CTGAGCGAGT CCGCATCGAC CGGATCGGAA AACCTCTCGA GAAAGGCGTC TAACCAGTCA CAGTCGCAAG GTAGGCTGAG CACCGTGGCG GGCGGCAGCG GGCGGCGGTC GGGGTTGTTT CTGGCGGAGG TGCTGCTGAT GATGTAATTA AAGTAGGCGG TCTTGAGACG GCGGATGGTC GACAGAAGCA CCATGTCCTT GGGTCCGGCC TGCTGAATGC GCAGGCGGTC GGCCATGCCC CAGGCTTCGT TTTGACATCG GCGCAGGTCT TTGTAGTAGT CTTGCATGAG CCTTTCTACC GGCACTTCTT CTTCTCCTTC CTCTTGTCCT GCATCTCTTG CATCTATCGC TGCGGCGGCG GCGGAGTTTG GCCGTAGGTG GCGCCCTCTT CCTCCCATGC GTGTGACCCC GAAGCCCCTC ATCGGCTGAA GCAGGGCTAG GTCGGCGACA ACGCGCTCGG CTAATATGGC CTGCTGCACC TGCGTGAGGG TAGACTGGAA GTCATCCATG TCCACAAAGC GGTGGTATGC GCCCGTGTTG ATGGTGTAAG TGCAGTTGGC CATAACGGAC CAGTTAACGG TCTGGTGACC CGGCTGCGAG AGCTCGGTGT ACCTGAGACG CGAGTAAGCC CTCGAGTCAA ATACGTAGTC GTTGCAAGTC CGCACCAGGT ACTGGTATCC CACCAAAAAG TGCGGCGGCG GCTGGCGGTA GAGGGGCCAG CGTAGGGTGG CCGGGGCTCC GGGGGCGAGA TCTTCCAACA TAAGGCGATG ATATCCGTAG ATGTACCTGG ACATCCAGGT GATGCCGGCG GCGGTGGTGG AGGCGCGCGG AAAGTCGCGG ACGCGGTTCC AGATGTTGCG CAGCGGCAAA AAGTGCTCCA TGGTCGGGAC GCTCTGGCCG GTCAGGCGCG CGCAATCGTT GACGCTCTAG CGTGCAAAAG GAGAGCCTGT AAGCGGGCAC TCTTCCGTGG TCTGGTGGAT AAATTCGCAA GGGTATCATG GCGGACGACC GGGGTTCGAG CCCCGTATCC GGCCGTCCGC CGTGATCCAT GCGGTTACCG CCCGCGTGTC GAACCCAGGT GTGCGACGTC AGACAACGGG GGAGTGCTCC TTTTGGCTTC CTTCCAGGCG CGGCGGCTGC TGCGCTAGCT TTTTTGGCCA CTGGCCGCGC GCAGCGTAAG CGGTTAGGCT GGAAAGCGAA AGCATTAAGT GGCTCGCTCC CTGTAGCCGG AGGGTTATTT TCCAAGGGTT GAGTCGCGGG ACCCCCGGTT CGAGTCTCGG ACCGGCCGGA CTGCGGCGAA CGGGGGTTTG CCTCCCCGTC ATGCAAGACC CCGCTTGCAA ATTCCTCCGG AAACAGGGAC GAGCCCCTTT TTTGCTTTTC CCAGATGCAT CCGGTGCTGC GGCAGATGCG CCCCCCTCCT CAGCAGCGGC AAGAGCAAGA GCAGCGGCAG ACATGCAGGG CACCCTCCCC TCCTCCTACC GCGTCAGGAG GGGCGACATC CGCGGTTGAC GCGGCAGCAG ATGGTGATTA CGAACCCCCG CGGCGCCGGG CCCGGCACTA CCTGGACTTG GAGGAGGGCG AGGGCCTGGC GCGGCTAGGA GCGCCCTCTC CTGAGCGGCA CCCAAGGGTG CAGCTGAAGC GTGATACGCG TGAGGCGTAC GTGCCGCGGC AGAACCTGTT TCGCGACCGC GAGGGAGAGG AGCCCGAGGA GATGCGGGAT CGAAAGTTCC ACGCAGGGCG CGAGCTGCGG CATGGCCTGA ATCGCGAGCG GTTGCTGCGC GAGGAGGACT TTGAGCCCGA CGCGCGAACC GGGATTAGTC CCGCGCGCGC ACACGTGGCG GCCGCCGACC TGGTAACCGC ATACGAGCAG ACGGTGAACC AGGAGATTAA CTTTCAAAAA AGCTTTAACA ACCACGTGCG TACGCTTGTG GCGCGCGAGG AGGTGGCTAT AGGACTGATG CATCTGTGGG ACTTTGTAAG CGCGCTGGAG CAAAACCCAA ATAGCAAGCC GCTCATGGCG CAGCTGTTCC TTATAGTGCA GCACAGCAGG GACAACGAGG CATTCAGGGA TGCGCTGCTA AACATAGTAG AGCCCGAGGG CCGCTGGCTG CTCGATTTGA TAAACATCCT GCAGAGCATA GTGGTGCAGG AGCGCAGCTT GAGCCTGGCT GACAAGGTGG CCGCCATCAA CTATTCCATG CTTAGCCTGG GCAAGTTTTA CGCCCGCAAG ATATACCATA CCCCTTACGT TCCCATAGAC AAGGAGGTAA AGATCGAGGG GTTCTACATG CGCATGGCGC TGAAGGTGCT TACCTTGAGC GACGACCTGG GCGTTTATCG CAACGAGCGC ATCCACAAGG CCGTGAGCGT GAGCCGGCGG CGCGAGCTCA GCGACCGCGA GCTGATGCAC AGCCTGCAAA GGGCCCTGGC TGGCACGGGC AGCGGCGATA GAGAGGCCGA GTCCTACTTT GACGCGGGCG CTGACCTGCG CTGGGCCCCA AGCCGACGCG CCCTGGAGGC AGCTGGGGCC GGACCTGGGC TGGCGGTGGC ACCCGCGCGC GCTGGCAACG TCGGCGGCGT GGAGGAATAT GACGAGGACG ATGAGTACGA GCCAGAGGAC GGCGAGTACT AAGCGGTGAT GTTTCTGATC AGATGATGCA AGACGCAACG GACCCGGCGG TGCGGGCGGC GCTGCAGAGC CAGCCGTCCG GCCTTAACTC CACGGACGAC TGGCGCCAGG TCATGGACCG CATCATGTCG CTGACTGCGC GCAATCCTGA CGCGTTCCGG CAGCAGCCGC AGGCCAACCG GCTCTCCGCA ATTCTGGAAG CGGTGGTCCC GGCGCGCGCA AACCCCACGC ACGAGAAGGT GCTGGCGATC GTAAACGCGC TGGCCGAAAA CAGGGCCATC CGGCCCGACG AGGCCGGCCT GGTCTACGAC GCGCTGCTTC AGCGCGTGGC TCGTTACAAC AGCGGCAACG TGCAGACCAA CCTGGACCGG CTGGTGGGGG ATGTGCGCGA GGCCGTGGCG CAGCGTGAGC GCGCGCAGCA GCAGGGCAAC CTGGGCTCCA TGGTTGCACT AAACGCCTTC CTGAGTACAC AGCCCGCCAA CGTGCCGCGG GGACAGGAGG ACTACACCAA CTTTGTGAGC GCACTGCGGC TAATGGTGAC TGAGACACCG CAAAGTGAGG TGTACCAGTC TGGGCCAGAC TATTTTTTCC AGACCAGTAG ACAAGGCCTG CAGACCGTAA ACCTGAGCCA GGCTTTCAAA AACTTGCAGG GGCTGTGGGG GGTGCGGGCT CCCACAGGCG ACCGCGCGAC CGTGTCTAGC TTGCTGACGC CCAACTCGCG CCTGTTGCTG CTGCTAATAG CGCCCTTCAC GGACAGTGGC AGCGTGTCCC GGGACACATA CCTAGGTCAC TTGCTGACAC TGTACCGCGA GGCCATAGGT CAGGCGCATG TGGACGAGCA TACTTTCCAG GAGATTACAA GTGTCAGCCG CGCGCTGGGG CAGGAGGACA CGGGCAGCCT GGAGGCAACC CTAAACTACC TGCTGACCAA CCGGCGGCAG AAGATCCCCT CGTTGCACAG TTTAAACAGC GAGGAGGAGC GCATTTTGCG CTACGTGCAG CAGAGCGTGA GCCTTAACCT GATGCGCGAC GGGGTAACGC CCAGCGTGGC GCTGGACATG ACCGCGCGCA ACATGGAACC GGGCATGTAT GCCTCAAACC GGCCGTTTAT CAACCGCCTA ATGGACTACT TGCATCGCGC GGCCGCCGTG AACCCCGAGT ATTTCACCAA TGCCATCTTG AACCCGCACT GGCTACCGCC CCCTGGTTTC TACACCGGGG GATTCGAGGT GCCCGAGGGT AACGATGGAT TCCTCTGGGA CGACATAGAC GACAGCGTGT TTTCCCCGCA ACCGCAGACC CTGCTAGAGT TGCAACAGCG CGAGCAGGCA GAGGCGGCGC TGCGAAAGGA AAGCTTCCGC AGGCCAAGCA GCTTGTCCGA TCTAGGCGCT GCGGCCCCGC GGTCAGATGC TAGTAGCCCA TTTCCAAGCT TGATAGGGTC TCTTACCAGC ACTCGCACCA CCCGCCCGCG CCTGCTGGGC GAGGAGGAGT ACCTAAACAA CTCGCTGCTG CAGCCGCAGC GCGAAAAAAA CCTGCCTCCG GCATTTCCCA ACAACGGGAT AGAGAGCCTA GTGGACAAGA TGAGTAGATG GAAGACGTAC GCGCAGGAGC ACAGGGACGT GCCAGGCCCG CGCCCGCCCA CCCGTCGTCA AAGGCACGAC CGTCAGCGGG GTCTGGTGTG GGAGGACGAT GACTCGGCAG ACGACAGCAG CGTCCTGGAT TTGGGAGGGA GTGGCAACCC GTTTGCGCAC CTTCGCCCCA GGCTGGGGAG AATGTTTTAA AAAAAAAAAA GCATGATGCA AAATAAAAAA CTCACCAAGG CCATGGCACC GAGCGTTGGT TTTCTTGTAT TCCCCTTAGT ATGCGGCGCG CGGCGATGTA TGAGGAAGGT CCTCCTCCCT CCTACGAGAG TGTGGTGAGC GCGGCGCCAG TGGCGGCGGC GCTGGGTTCT CCCTTCGATG CTCCCCTGGA CCCGCCGTTT GTGCCTCCGC GGTACCTGCG GCCTACCGGG GGGAGAAACA GCATCCGTTA CTCTGAGTTG GCACCCCTAT TCGACACCAC CCGTGTGTAC CTGGTGGACA ACAAGTCAAC GGATGTGGCA TCCCTGAACT ACCAGAACGA CCACAGCAAC TTTCTGACCA CGGTCATTCA AAACAATGAC TACAGCCCGG GGGAGGCAAG CACACAGACC ATCAATCTTG ACGACCGGTC GCACTGGGGC GGCGACCTGA AAACCATCCT GCATACCAAC ATGCCAAATG TGAACGAGTT CATGTTTACC AATAAGTTTA AGGCGCGGGT GATGGTGTCG CGCTTGCCTA CTAAGGACAA TCAGGTGGAG CTGAAATACG AGTGGGTGGA GTTCACGCTG CCCGAGGGCA ACTACTCCGA GACCATGACC ATAGACCTTA TGAACAACGC GATCGTGGAG CACTACTTGA AAGTGGGCAG ACAGAACGGG GTTCTGGAAA GCGACATCGG GGTAAAGTTT GACACCCGCA ACTTCAGACT GGGGTTTGAC CCCGTCACTG GTCTTGTCAT GCCTGGGGTA TATACAAACG AAGCCTTCCA TCCAGACATC ATTTTGCTGC CAGGATGCGG GGTGGACTTC ACCCACAGCC GCCTGAGCAA CTTGTTGGGC ATCCGCAAGC GGCAACCCTT CCAGGAGGGC TTTAGGATCA CCTACGATGA TCTGGAGGGT GGTAACATTC CCGCACTGTT GGATGTGGAC GCCTACCAGG CGAGCTTGAA AGATGACACC GAACAGGGCG GGGGTGGCGC AGGCGGCAGC AACAGCAGTG GCAGCGGCGC GGAAGAGAAC TCCAACGCGG CAGCCGCGGC AATGCAGCCG GTGGAGGACA TGAACGATCA TGCCATTCGC GGCGACACCT TTGCCACACG GGCTGAGGAG AAGCGCGCTG AGGCCGAAGC AGCGGCCGAA GCTGCCGCCC CCGCTGCGCA ACCCGAGGTC GAGAAGCCTC AGAAGAAACC GGTGATCAAA CCCCTGACAG AGGACAGCAA GAAACGCAGT TACAACCTAA TAAGCAATGA CAGCACCTTC ACCCAGTACC GCAGCTGGTA CCTTGCATAC AACTACGGCG ACCCTCAGAC CGGAATCCGC TCATGGACCC TGCTTTGCAC TCCTGACGTA ACCTGCGGCT CGGAGCAGGT CTACTGGTCG TTGCCAGACA TGATGCAAGA CCCCGTGACC TTCCGCTCCA CGCGCCAGAT CAGCAACTTT CCGGTGGTGG GCGCCGAGCT GTTGCCCGTG CACTCCAAGA GCTTCTACAA CGACCAGGCC GTCTACTCCC AACTCATCCG CCAGTTTACC TCTCTGACCC ACGTGTTCAA TCGCTTTCCC GAGAACCAGA TTTTGGCGCG CCCGCCAGCC CCCACCATCA CCACCGTCAG TGAAAACGTT CCTGCTCTCA CAGATCACGG GACGCTACCG CTGCGCAACA GCATCGGAGG AGTCCAGCGA GTGACCATTA CTGACGCCAG ACGCCGCACC TGCCCCTACG TTTACAAGGC CCTGGGCATA GTCTCGCCGC GCGTCCTATC GAGCCGCACT TTTTGAGCAA GCATGTCCAT CCTTATATCG CCCAGCAATA ACACAGGCTG GGGCCTGCGC TTCCCAAGCA AGATGTTTGG CGGGGCCAAG AAGCGCTCCG ACCAACACCC AGTGCGCGTG CGCGGGCACT ACCGCGCGCC CTGGGGCGCG CACAAACGCG GCCGCACTGG GCGCACCACC GTCGATGACG CCATCGACGC GGTGGTGGAG GAGGCGCGCA ACTACACGCC CACGCCGCCA CCAGTGTCCA CAGTGGACGC GGCCATTCAG ACCGTGGTGC GCGGAGCCCG GCGCTATGCT AAAATGAAGA GACGGCGGAG GCGCGTAGCA CGTCGCCACC GCCGCCGACC CGGCACTGCC GCCCAACGCG CGGCGGCGGC CCTGCTTAAC CGCGCACGTC GCACCGGCCG ACGGGCGGCC ATGCGGGCCG CTCGAAGGCT GGCCGCGGGT ATTGTCACTG TGCCCCCCAG GTCCAGGCGA CGAGCGGCCG CCGCAGCAGC CGCGGCCATT AGTGCTATGA CTCAGGGTCG CAGGGGCAAC GTGTATTGGG TGCGCGACTC GGTTAGCGGC CTGCGCGTGC CCGTGCGCAC CCGCCCCCCG CGCAACTAGA TTGCAAGAAA AAACTACTTA GACTCGTACT GTTGTATGTA TCCAGCGGCG GCGGCGCGCA ACGAAGCTAT GTCCAAGCGC AAAATCAAAG AAGAGATGCT CCAGGTCATC GCGCCGGAGA TCTATGGCCC CCCGAAGAAG GAAGAGCAGG ATTACAAGCC CCGAAAGCTA AAGCGGGTCA AAAAGAAAAA GAAAGATGAT GATGATGAAC TTGACGACGA GGTGGAACTG CTGCACGCTA CCGCGCCCAG GCGACGGGTA CAGTGGAAAG GTCGACGCGT AAAACGTGTT TTGCGACCCG GCACCACCGT AGTCTTTACG CCCGGTGAGC GCTCCACCCG CACCTACAAG CGCGTGTATG ATGAGGTGTA CGGCGACGAG GACCTGCTTG AGCAGGCCAA CGAGCGCCTC GGGGAGTTTG CCTACGGAAA GCGGCATAAG GACATGCTGG CGTTGCCGCT GGACGAGGGC AACCCAACAC CTAGCCTAAA GCCCGTAACA CTGCAGCAGG TGCTGCCCGC GCTTGCACCG TCCGAAGAAA AGCGCGGCCT AAAGCGCGAG TCTGGTGACT TGGCACCCAC CGTGCAGCTG ATGGTACCCA AGCGCCAGCG ACTGGAAGAT GTCTTGGAAA AAATGACCGT GGAACCTGGG CTGGAGCCCG AGGTCCGCGT GCGGCCAATC AAGCAGGTGG CGCCGGGACT GGGCGTGCAG ACCGTGGACG TTCAGATACC CACTACCAGT AGCACCAGTA TTGCCACCGC CACAGAGGGC ATGGAGACAC AAACGTCCCC GGTTGCCTCA GCGGTGGCGG ATGCCGCGGT GCAGGCGGTC GCTGCGGCCG CGTCCAAGAC CTCTACGGAG GTGCAAACGG ACCCGTGGAT GTTTCGCGTT TCAGCCCCCC GGCGCCCGCG CCGTTCGAGG AAGTACGGCG CCGCCAGCGC GCTACTGCCC GAATATGCCC TACATCCTTC CATTGCGCCT ACCCCCGGCT ATCGTGGCTA CACCTACCGC CCCAGAAGAC GAGCAACTAC CCGACGCCGA ACCACCACTG GAACCCGCCG CCGCCGTCGC CGTCGCCAGC CCGTGCTGGC CCCGATTTCC GTGCGCAGGG TGGCTCGCGA AGGAGGCAGG ACCCTGGTGC TGCCAACAGC GCGCTACCAC CCCAGCATCG TTTAAAAGCC GGTCTTTGTG GTTCTTGCAG ATATGGCCCT CACCTGCCGC CTCCGTTTCC CGGTGCCGGG ATTCCGAGGA AGAATGCACC GTAGGAGGGG CATGGCCGGC CACGGCCTGA CGGGCGGCAT GCGTCGTGCG CACCACCGGC GGCGGCGCGC GTCGCACCGT CGCATGCGCG GCGGTATCCT GCCCCTCCTT ATTCCACTGA TCGCCGCGGC GATTGGCGCC GTGCCCGGAA TTGCATCCGT GGCCTTGCAG GCGCAGAGAC ACTGATTAAA AACAAGTTGC ATGTGGAAAA ATCAAAATAA AAAGTCTGGA CTCTCACGCT CGCTTGGTCC TGTAACTATT TTGTAGAATG GAAGACATCA ACTTTGCGTC TCTGGCCCCG CGACACGGCT CGCGCCCGTT CATGGGAAAC TGGCAAGATA TCGGCACCAG CAATATGAGC GGTGGCGCCT TCAGCTGGGG CTCGCTGTGG AGCGGCATTA AAAATTTCGG TTCCACCGTT AAGAACTATG GCAGCAAGGC CTGGAACAGC AGCACAGGCC AGATGCTGAG GGATAAGTTG AAAGAGCAAA ATTTCCAACA AAAGGTGGTA GATGGCCTGG CCTCTGGCAT TAGCGGGGTG GTGGACCTGG CCAACCAGGC AGTGCAAAAT AAGATTAACA GTAAGCTTGA TCCCCGCCCT CCCGTAGAGG AGCCTCCACC GGCCGTGGAG ACAGTGTCTC CAGAGGGGCG TGGCGAAAAG CGTCCGCGCC CCGACAGGGA AGAAACTCTG GTGACGCAAA TAGACGAGCC TCCCTCGTAC GAGGAGGCAC TAAAGCAAGG CCTGCCCACC ACCCGTCCCA TCGCGCCCAT GGCTACCGGA GTGCTGGGCC AGCACACACC CGTAACGCTG GACCTGCCTC CCCCCGCCGA CACCCAGCAG AAACCTGTGC TGCCAGGCCC GACCGCCGTT GTTGTAACCC GTCCTAGCCG CGCGTCCCTG CGCCGCGCCG CCAGCGGTCC GCGATCGTTG CGGCCCGTAG CCAGTGGCAA CTGGCAAAGC ACACTGAACA GCATCGTGGG TCTGGGGGTG CAATCCCTGA AGCGCCGACG ATGCTTCTGA TAGCTAACGT GTCGTATGTG TGTCATGTAT GCGTCCATGT CGCCGCCAGA GGAGCTGCTG AGCCGCCGCG CGCCCGCTTT CCAAGATGGC TACCCCTTCG ATGATGCCGC AGTGGTCTTA CATGCACATC TCGGGCCAGG ACGCCTCGGA GTACCTGAGC CCCGGGCTGG TGCAGTTTGC CCGCGCCACC GAGACGTACT TCAGCCTGAA TAACAAGTTT AGAAACCCCA CGGTGGCGCC TACGCACGA
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