pROKII-RNAI
$99.00
In stock
SKU
PL82471
100μg liquid plasmid(Instantly):$120
| name | pROKII-RNAI | another name | apROKII-RNAI |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Interfere with silence | Fragment type | shRNA |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | 35s | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | 35s： | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAGCGTCGC AAAGGCGCTC GGTCTTGCCT TGCTCGTCGG TGATGTACTT CACCAGCTCC GCGAAGTCGC TCTTCTTGAT GGAGCGCATG GGGACGTGCT TGGCAATCAC GCGCACCCCC CGGCCGTTTT AGCGGCTAAA AAAGTCATGG CTCTGCCCTC GGGCGGACCA CGCCCATCAT GACCTTGCCA AGCTCGTCCT GCTTCTCTTC GATCTTCGCC AGCAGGGCGA GGATCGTGGC ATCACCGAAC CGCGCCGTGC GCGGGTCGTC GGTGAGCCAG AGTTTCAGCA GGCCGCCCAG GCGGCCCAGG TCGCCATTGA TGCGGGCCAG CTCGCGGACG TGCTCATAGT CCACGACGCC CGTGATTTTG TAGCCCTGGC CGACGGCCAG CAGGTAGGCC GACAGGCTCA TGCCGGCCGC CGCCGCCTTT TCCTCAATCG CTCTTCGTTC GTCTGGAAGG CAGTACACCT TGATAGGTGG GCTGCCCTTC CTGGTTGGCT TGGTTTCATC AGCCATCCGC TTGCCCTCAT CTGTTACGCC GGCGGTAGCC GGCCAGCCTC GCAGAGCAGG ATTCCCGTTG AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC GGGTGGGCCT ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCAGA AGGCCGCCAG AGAGGCCGAG CGCGGCCGTG AGGCTTGGAC GCTAGGGCAG GGCATGAAAA AGCCCGTAGC GGGCTGCTAC GGGCGTCTGA CGCGGTGGAA AGGGGGAGGG GATGTTGTCT ACATGGCTCT GCTGTAGTGA GTGGGTTGCG CTCCGGCAGC GGTCCTGATC AATCGTCACC CTTTCTCGGT CCTTCAACGT TCCTGACAAC GAGCCTCCTT TTCGCCAATC CATCGACAAT CACCGCGAGT CCCTGCTCGA ACGCTGCGTC CGGACCGGCT TCGTCGAAGG CGTCTATCGC GGCCCGCAAC AGCGGCGAGA GCGGAGCCTG TTCAACGGTG CCGCCGCGCT CGCCGGCATC GCTGTCGCCG GCCTGCTCCT CAAGCACGGC CCCAACAGTG AAGTAGCTGA TTGTCATCAG CGCATTGACG GCGTCCCCGG CCGAAAAACC CGCCTCGCAG AGGAAGCGAA GCTGCGCGTC GGCCGTTTCC ATCTGCGGTG CGCCCGGTCG CGTGCCGGCA TGGATGCGCG CGCCATCGCG GTAGGCGAGC AGCGCCTGCC TGAAGCTGCG GGCATTCCCG ATCAGAAATG AGCGCCAGTC GTCGTCGGCT CTCGGCACCG AATGCGTATG ATTCTCCGCC AGCATGGCTT CGGCCAGTGC GTCGAGCAGC GCCCGCTTGT TCCTGAAGTG CCAGTAAAGC GCCGGCTGCT GAACCCCCAA CCGTTCCGCC AGTTTGCGTG TCGTCAGACC GTCTACGCCG ACCTCGTTCA ACAGGTCCAG GGCGGCACGG ATCACTGTAT TCGGCTGCAA CTTTGTCATG CTTGACACTT TATCACTGAT AAACATAATA TGTCCACCAA CTTATCAGTG ATAAAGAATC CGCGCGTTCA ATCGGACCAG CGGAGGCTGG TCCGGAGGCC AGACGTGAAA CCCAACATAC CCCTGATCGT AATTCTGAGC ACTGTCGCGC TCGACGCTGT CGGCATCGGC CTGATTATGC CGGTGCTGCC GGGCCTCCTG CGCGATCTGG TTCACTCGAA CGACGTCACC GCCCACTATG GCATTCTGCT GGCGCTGTAT GCGTTGGTGC AATTTGCCTG CGCACCTGTG CTGGGCGCGC TGTCGGATCG TTTCGGGCGG CGGCCAATCT TGCTCGTCTC GCTGGCCGGC GCCAGATCTG GGGAACCCTG TGGTTGGCAT GCACATACAA ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA CGTTGAAGGA GCCACTCAGC CGCGGGTTTC TGGAGTTTAA TGAGCTAAGC ACATACGTCA GAAACCATTA TTGCGCGTTC AAAAGTCGCC TAAGGTCACT ATCAGCTAGC AAATATTTCT TGTCAAAAAT GCTCCACTGA CGTTCCATAA ATTCCCCTCG GTATCCAATT AGAGTCTCAT ATTCACTCTC AATCCAAATA ATCTGCACCG GATCTGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GATGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACGGGATCTC TGCGGAACAG GCGGTCGAAG GTGCCGATAT CATTACGACA GCAACGGCCG ACAAGCACAA CGCCACGATC CTGAGCGACA ATATGATCGG GCCCGGCGTC CACATCAACG GCGTCGGCGG CGACTGCCCA GGCAAGACCG AGATGCACCG CGATATCTTG CTGCGTTCGG ATATTTTCGT GGAGTTCCCG CCACAGACCC GGATGATCCC CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGGGCCT CCTGTCAATG CTGGCGGCGG CTCTGGTGGT GGTTCTGGTG GCGGCTCTGA GGGTGGTGGC TCTGAGGGTG GCGGTTCTGA GGGTGGCGGC TCTGAGGGAG GCGGTTCCGG TGGTGGCTCT GGTTCCGGTG ATTTTGATTA TGAAAAGATG GCAAACGCTA ATAAGGGGGC TATGACCGAA AATGCCGATG AAAACGCGCT ACAGTCTGAC GCTAAAGGCA AACTTGATTC TGTCGCTACT GATTACGGTG CTGCTATCGA TGGTTTCATT GGTGACGTTT CCGGCCTTGC TAATGGTAAT GGTGCTACTG GTGATTTTGC TGGCTCTAAT TCCCAAATGG CTCAAGTCGG TGACGGTGAT AATTCACCTT TAATGAATAA TTTCCGTCAA TATTTACCTT CCCTCCCTCA ATCGGTTGAA TGTCGCCCTT TTGTCTTTGG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC CCCAGATTAG CCTTTTCAAT TTCAGAAAGA ATGCTAACCC ACAGATGGTT AGAGAGGCTT ACGCAGCAGG TCTCATCAAG ACGATCTACC CGAGCAATAA TCTCCAGGAA ATCAAATACC TTCCCAAGAA GGTTAAAGAT GCAGTCAAAA GATTCAGGAC TAACTGCATC AAGAACACAG AGAAAGATAT ATTTCTCAAG ATCAGAAGTA CTATTCCAGT ATGGACGATT CAAGGCTTGC TTCACAAACC AAGGCAAGTA ATAGAGATTG GAGTCTCTAA AAAGGTAGTT CCCACTGAAT CAAAGGCCAT GGAGTCAAAG ATTCAAATAG AGGACCTAAC AGAACTCGCC GTAAAGACTG GCGAACAGTT CATACAGAGT CTCTTACGAC TCAATGACAA GAAGAAAATC TTCGTCAACA TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGAACACGGG GGACTCTAGA GGATCCTACC CGGGGGTAAA TTTCTAGTTT TTCTCCTTCA TTTTCTTGGT TAGGACCCTT TTCTCTTTTT ATTTTTTTGA GCTTTGATCT TTCTTTAAAC TGATCTATTT TTTAATTGAT TGGTTATGGT GTAAATATTA CATAGCTTTA ACTGATAATC TGATTACTTT ATTTCGTGTG TCTATGATGA TGATGATAGT TACAGAACTA GTCAGGTACC GAGCTCGAAT TTCCCCGATC GTTCAAACAT TTGGCAATAA AGTTTCTTAA GATTGAATCC TGTTGCCGGT CTTGCGATGA TTATCATATA ATTTCTGTTG AATTACGTTA AGCATGTAAT AATTAACATG TAATGCATGA CGTTATTTAT GAGATGGGTT TTTATGATTA GAGTCCCGCA ATTATACATT TAATACGCGA TAGAAAACAA AATATAGCGC GCAAACTAGG ATAAATTATC GCGCGCGGTG TCATCTATGT TACTAGATCG GGAATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTTGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGGC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGAAC CACCATCAAA CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG TGAAAAGAAA AACCACCCCA GTACATTAAA AACGTCCGCA ATGTGTTATT AAGTTGTCTA AGCGTCAATT TGTTTACACC ACAATATATC CTGCCACCAG CCAGCCAACA GCTCCCCGAC CGGCAGCTCG GCACAAAATC ACCACTCGAT ACAGGCAGCC CATCAGTCCG GGACGGCGTC AGCGGGAGAG CCGTTGTAAG GCGGCAGACT TTGCTCATGT TACCGATGCT ATTCGGAAGA ACGGCAACTA AGCTGCCGGG TTTGAAACAC GGATGATCTC GCGGAGGGTA GCATGTTGAT TGTAACGATG ACAGAGCGTT GCTGCCTGTG ATCAAATATC ATCTCCCTCG CAGAGATCCG AATTATCAGC CTTCTTATTC ATTTCTCGCT TAACCGTGAC AGGCTGTCGA TCTTGAGAAC TATGCCGACA TAATAGGAAA TCGCTGGATA AAGCCGCTGA GGAAGCTGAG TGGCGCTATT TCTTTAGAAG TGAACGTTGA CGATATCAAC TCCCCTATCC ATTGCTCACC GAATGGTACA GGTCGGGGAC CCGAAGTTCC GACTGTCGGC CTGATGCATC CCCGGCTGAT CGACCCCAGA TCTGGGGCTG AGAAAGCCCA GTAAGGAAAC AACTGTAGGT TCGAGTCGCG AGATCCCCCG GAACCAAAGG AAGTAGGTTA AACCCGCTCC GATCAGGCCG AGCCACGCCA GGCCGAGAAC ATTGGTTCCT GTAGGCATCG GGATTGGCGG ATCAAACACT AAAGCTACTG GAACGAGCAG AAGTCCTCCG GCCGCCAGTT GCCAGGCGGT AAAGGTGAGC AGAGGCACGG GAGGTTGCCA CTTGCGGGTC AGCACGGTTC CGAACGCCAT GGAAACCGCC CCCGCCAGGC CCGCTGCGAC GCCGACAGGA TCTAGCGCTG CGTTTGGTGT CAACACCAAC AGCGCCACGC CCGCAGTTCC GCAAATAGCC CCCAGGACCG CCATCAATCG TATCGGGCTA CCTAGCAGAG CGGCAGAGAT GAACACGACC ATCAGCGGCT GCACAGCGCC TACCGTCGCC GCGACCCCGC CCGGCAGGCG GTAGACCGAA ATAAACAACA AGCTCCAGAA TAGCGAAATA TTAAGTGCGC CGAGGATGAA GATGCGCATC CACCAGATTC CCGTTGGAAT CTGTCGGACG ATCATCACGA GCAATAAACC CGCCGGCAAC GCCCGCAGCA GCATACCGGC GACCCCTCGG CCTCGCTGTT CGGGCTCCAC GAAAACGCCG GACAGATGCG CCTTGTGAGC GTCCTTGGGG CCGTCCTCCT GTTTGAAGAC CGACAGCCCA ATGATCTCGC CGTCGATGTA GGCGCCGAAT GCCACGGCAT CTCGCAACCG TTCAGCGAAC GCCTCCATGG GCTTTTTCTC CTCGTGCTCG TAAACGGACC CGAACATCTC TGGAGCTTTC TTCAGGGCCG ACAATCGGAT CTCGCGGAAA TCCTGCACGT CGGCCGCTCC AAGCCGTCGA ATCTGAGCCT TAATCACAAT TGTCAATTTT AATCCTCTGT TTATCGGCAG TTCGTAGAGC GCGCCGTGCG TCCCGAGCGA TACTGAGCGA AGCAAGTGCG TCGAGCAGTG CCCGCTTGTT CCTGAAATGC CAGTAAAGCG CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT GCAATGCACC AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC TTCGCAGGCT TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA CATGAGGCGG AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC GAACATCCGT CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA GTGGTCGCCA GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT TTTCTTGCCA CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC AACGCGGTCG GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC CTTCGTGTAA TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT GAAGGTGATC GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC CAGTTCGTCA TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT GACGTGGAAA ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT GAACAGGGCA GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC AATATCGAAC AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC GGCCTGCTTG GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT GGTCGTCATA GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG TTCGAGACGA CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG TTGCACGCTG TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG GAAGGTTTCG CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC GGAAAACCCC GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT TCACCCTCCT TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT TGACCCGCCT GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC GTAGACCGTC TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC CAGCGACCCC TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT GCGGAAGAAG TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACA TCTAGGTACT AAAACAATTC ATCCAGTAAA ATATAATATT TTATTTTCTC CCAATCAGGC TTGATCCCCA GTAAGTCAAA AAATAGCTCG ACATACTGTT CTTCCCCGAT ATCCTCCCTG ATCGACCGGA CGCAGAAGGC AATGTCATAC CACTTGTCCG CCCTGCCGCT TCTCCCAAGA TCAATAAAGC CACTTACTTT GCCATCTTTC ACAAAGATGT TGCTGTCTCC CAGGTCGCCG TGGGAAAAGA CAAGTTCCTC TTCGGGCTTT TCCGTCTTTA AAAAATCATA CAGCTCGCGC GGATCTTTAA ATGGAGTGTC TTCTTCCCAG TTTTCGCAAT CCACATCGGC CAGATCGTTA TTCAGTAAGT AATCCAATTC GGCTAAGCGG CTGTCTAAGC TATTCGTATA GGGACAATCC GATATGTCGA TGGAGTGAAA GAGCCTGATG CACTCCGCAT ACAGCTCGAT AATCTTTTCA GGGCTTTGTT CATCTTCATA CTCTTCCGAG CAAAGGACGC CATCGGCCTC ACTCATGAGC AGATTGCTCC AGCCATCATG CCGTTCAAAG TGCAGGACCT TTGGAACAGG CAGCTTTCCT TCCAGCCATA GCATCATGTC CTTTTCCCGT TCCACATCAT AGGTGGTCCC TTTATACCGG CTGTCCGTCA TTTTTAAATA TAGGTTTTCA TTTTCTCCCA CCAGCTTATA TACCTTAGCA GGAGACATTC CTTCCGTATC TTTTACGCAG CGGTATTTTT CGATCAGTTT TTTCAATTCC GGTGATATTC TCATTTTAGC CATTTATTAT TTCCTTCCTC TTTTCTACAG TATTTAAAGA TACCCCAAGA AGCTAATTAT AACAAGACGA ACTCCAATTC ACTGTTCCTT GCATTCTAAA ACCTTAAATA CCAGAAAACA GCTTTTTCAA AGTTGTTTTC AAAGTTGGCG TATAACATAG TATCGACGGA GCCGATTTTG AAACCACAAT TATGGGTGAT GCTGCCAACT TACTGATTTA GTGTATGATG GTGTTTTTGA GGTGCTCCAG TGGCTTCTGT GTCTATCAGC TGTCCCTCCT GTTCAGCTAC TGACGGGGTG GTGCGTAACG GCAAAAGCAC CGCCGGACAT CAGCGCTATC TCTGCTCTCA CTGCCGTAAA ACATGGCAAC TGCAGTTCAC TTACACCGCT TCTCAACCCG GTACGCACCA GAAAATCATT GATATGGCCA TGAATGGCGT TGGATGCCGG GCAACAGCCC GCATTATGGG CGTTGGCCTC AACACGATTT TACGTCACTT AAAAAACTCA GGCCGCAGTC GGTAACCTCG CGCATACAGC CGGGCAGTGA CGTCATCGTC TGCGCGGAAA TGGACGAACA GTGGGGCTAT GTCGGGGCTA AATCGCGCCA GCGCTGGCTG TTTTACGCGT ATGACAGTCT CCGGAAGACG GTTGTTGCGC ACGTATTCGG TGAACGCACT ATGGCGACGC TGGGGCGTCT TATGAGCCTG CTGTCACCCT TTGACGTGGT GATATGGATG ACGGATGGCT GGCCGCTGTA TGAATCCCGC CTGAAGGGAA AGCTGCACGT AATCAGCAAG CGATATACGC AGCGAATTGA GCGGCATAAC CTGAATCTGA GGCAGCACCT GGCACGGCTG GGACGGAAGT CGCTGTCGTT CTCAAAATCG GTGGAGCTGC ATGACAAAGT CATCGGGCAT TATCTGAACA TAAAACACTA TCAATAAGTT GGAGTCATTA CCCAATTATG ATAGAATTTA CAAGCTATAA GGTTATTGTC CTGGGTTTCA AGCATTAGTC CATGCAAGTT TTTATGCTTT GCCCATTCTA TAGATATATT GATAAGCGCG CTGCCTATGC CTTGCCCCCT GAAATCCTTA CATACGGCGA TATCTTCTAT ATAAAAGATA TATTATCTTA TCAGTATTGT CAATATATTC AAGGCAATCT GCCTCCTCAT CCTCTTCATC CTCTTCGTCT TGGTAGCTTT TTAAATATGG CGCTTCATAG AGTAATTCTG TAAAGGTCCA ATTCTCGTTT CATACCTCGG TATAATCTTA CCTATCACCT CAAATGGTTC GCTGGGTTTA TCGCACCCCC GAACACGAGC ACGGCACCCG CGACCACTAT GCCAAGAATG CCCAAGGTAA AAATTGCCGG CCCCGCCATG AAGTCCGTGA ATGCCCCGAC GGCCGAAGTG AAGGGCAGGC CGCCACCAGG CCGCCGCCCT CACTGCCCGG CACCTGGTCG CTGAATGTCG ATGCCAGCAC CTGCGGCACG TCAATGCTTC CGGGCGTCGC GCTCGGGCTG ATCGCCCATC CCGTTACTGC CCCGATCCCG GCAATGGCAA GGACTGCCAG CGCTGCCATT TTTGGGGTGA GGCCGTTCGC GGCCGAGGGG CGCAGCCCCT GGGGGGATGG GAGGCCCGCG TTAGCGGGCC GGGAGGGTTC GAGAAGGGGG GGCACCCCCC TTCGGCGTGC GCGGTCACGC GCACAGGGCG CAGCCCTGGT TAAAAACAAG GTTTATAAAT ATTGGTTTAA AAGCAGGTTA AAAGACAGGT TAGCGGTGGC CGAAAAACGG GCGGAAACCC TTGCAAATGC TGGATTTTCT GCCTGTGGAC AGCCCCTCAA ATGTCAATAG GTGCGCCCCT CATCTGTCAG CACTCTGCCC CTCAAGTGTC AAGGATCGCG CCCCTATCTG TCAGTAGTCG CGCCCCTCAA GTGTCAATAC CGCAGGGCAC TTATCCCCAG GCTTGTCCAC ATCATCTGTG GGAAACTCGC GTAAAATCAG GCGTTTTCGC CGATTTGCGA GGCTGGCCAG CTCCACGTCG CCGGCCGAAA TCGAGCCTGC CCCTCATCTG TCAACGCCGC GCCGGGTGAG TCGGCCCCTC AAGTGTCAAC GTCCGCCCCT CATCTGTCAG TGAGGGCCAA GTTTTCCGCG AGGTATCCAC AACGCCGGCG GCCGCGGTGT CTCGCACACG GCTTCGACGG CGTTTCTGGC GCGTTTGCAG GGCCATAGAC GGCCGCCAGC CCAGCGGCGA GGGCAACCAG CCCGG
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