pGAL1-MCS-URA3
$99.00
In stock
SKU
PL82459
100μg liquid plasmid(Instantly):$120
| name | pGAL1-MCS-URA3 | another name | apGAL1-MCS-URA3 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | URA3 | Fluorescent labeling | |
| Promoter | GAL1 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | P0646-R：caaatatgcttcccagcct | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCA GAATTAACCC TCACTAAAGG GACTAGTTCA TACGTGGACA TTAGAACTCC CATTTCCACC TGCACCTTCC ATGCCTTCTG TAGATTGGGA GGAAGATGGG CGGGAGCTTC TGGGGCGAGG TTGACCATTT AGTCCCACTG GCGTGCCTAA ACCATGACTG ATCTGGGAAG CTGCATCATC GGATTCCCAG CGTTCCAACT CTTCACGTAC TAGCCGTTCC ATCTGTTCCC TATGGATTTC ACTGTCACGA CCCAGCCTTT CATCCTCGGC CACCCCATCC AACAGCCTTC CCAGCACCTC CCTCCTCTTC AGTTTGGCTC GCTCCATAAT CTCTAGCTTC ACGATCACGG CGTCAATCTT GGACACTATG CTGCCGATGG AATGCTCCAT CCGGTCCACT CGTCTCACCA GGACTTGAAA CTCTTCGTAA GAAACGCCAC TAGAAATGCT TCCCCTCCTT CTGGAGCTAT GTCCGCTATC TTCATCGTCA TCCTCCTCAG AGTCATCCAG GCTTCGAGGG AAACTTCGGC TGCTCATGGG ACGTGGTAAA GAACTGTGAT CCAAATCCAG GTCCTCCCTC TCTTTCTCCA AGTCGTCTCT CATCTGCTGA TGTTCATGTT CGGTCAGTTC TTGGTCTCCA TCTTGGTCGT ACTTTGTGAA TATTGCCTCA ATCTCTGCAT CAGTATGGCC CTTCCCTTTG AGATCTTGTC GAAGTTCGTC AAAGTTTAAC TTGCCTCCTC CTTGCCGCAG ACTCTCTGAA ATGTCATCCA CGGTATTTTT TTTCAGTTTT AGTTTGACCA AAGCTTTATG GTAGCCCTTT CTGATAAGAT CTGAGAGTTC CATTTCAGCT TTCTGCTGTG CCAAGTCAGA TTTCACTTCA GAGTAAGTAT CATTGATGAT AGCCAAAAAC ATATTCAAAA GAATGAAGAA CATAAAGAAC ACAAATGTAG TGAAATAAAT TGGTCCCAAA ACTCGATTAG CTTCCTCAAT CTCTGCAAAG TTGATATCGC CCAAAATGAT ACGGAATTGA GTGAAGATAC ACTCTTGGAA AGTACTGAAG TCATCGACCT GAGTGCCAAA GACAAGGTAT GCCAACTGAG CATACGCTAG GAAAATAATG AAGAACATAA TAGCAAAGCC AAACAGGTCT TTGGCACATC GAGACATGGT TGTCGAGAGC TGGCTCATGG TCCTGTTAAA ATTGATGAAT TTGAAGAGCT TAATCCAGAC AAAAAATACT GTGACAGCAG CTATATTGTT GAACTGTATC TGCCAATATG CCAGATGCTC AAAGTTGGGG AAAGTATTTT GATCTTCCAG AAACTGTAGT AGCACCTCCA CATTTGATGT TCTGTATATG TTAATTCCTA TAGCTACCAC TGACAGCACA ACGATCACAA CATCCAGACA ATTCCAGAAA CTCCTGAAAT AGTGTAGTTT GTGAATGCGA ATTTCCAATA TCTCTTCCAC CACATAGTAA AAGATAAAGA AACAAAAGAT AATCTCACAG GCTGCCAGGA AGAAATCAAA AGTTGTGACA TATCGGATCA GCTTTAAAGG CTGAAATTGC CAAGATGGAA TCACACCACC TGTTGCTGGG AATTCAACCA ATAACCTGAC CACACAGAAC AGGTTAATGT TGGCGTTGTA CACTGAGAAG TCAATAAAAG TTGCCCTGGT TCCTCGGTCC AGCCAGACAT TTTTCTTGAG GCTAGCAACT TGTGCAGCTG TTTCCTCTCT TGTTCTTGAC AAATCCAGAT AATAGCCAGC TCCACTATAA GTTGCAATGA TTCCCCAGTG GCTACTACCA TTCAAGTCTT TTTCACTTGT GTAGATCCAA GCGGTTCCAT TTCGGGGCCC AAAGGGAGCC CTATCTTCAC TACTGACAGA GTAGACATCA TAGCACTCTT TAATTTCATC TCTCAAGTCC TGGGGGATAG AGCAGGATCC ATTTCTGACT CGGAGTTGCC GTATTCGTGG AACCCCTAAC AGCAGGTTCT CATAGAAGAT GAAACTTCGG TTGTCAGCTT CAGTCTGGTT GCTGGGCTGC ATCTTCCAGT ACAGCCCATC CAATAAGGAG CCTTCTGTGA ACTTCCAGAA GTCTTCCATG GAAGACAGAG TTTTAAAGTT AGTTTTCTCC GTTTTGGACA CGGGGGTGTC TAGGAAGAGC TGTGACATCA TCCGGGTGTA GTAGTACACA TTGGAGCTCA TCATGCCGTA GGTCAAGATG CACAAGACTA TGAGAAAAAG GAGGTATGTG ACCAGTTCCC GTAAAACACT TTTAAGGTAT TTCTCTCGGT TAGTGCTGCT TTCCTCCATG AGTCTTGTTC CCCAGAGACC TCGCAGCCCG CGAACCAGCC TCTCCGCCCA GGCCACTCGG GGCGGCTGCC CTTCCAGGGG GAGGTGGCGA TGCAGCGGGT CCCCGCCGCC GACTGGGCTG GGGCACGGCG GGCCCTGGTC CTCTCGCCGG CGCCGCCTCC CGCTCGGGTG GCCCGCGCCG TGGTAGCCCC CAAGCCCCCG GCTCCGCGCG CCCACGGAGC TCACGGCCGA GGAGGCGGCC GACCTCCGGC TGCCCGGGCG CCACTCTACG TCCATCTCCA CCACCATTCC GCCTTCTTCC CCTTCCACCT CCTCCTCCTC CTCCTCGGCC TCGAAGCCGG GGTTATCGCG GCTCCACGCC TGCCGGGAGC ACGACGAGAG CGGAGGAGAA GGGGAGGCCG CGGCTCCGGC CGGGGGGTCC CGCGCGGCCG CCTGCCGGAT GCGCTGCATC TCGATCTCCA GGCCCCGCTG CTCGCAGAGG CCGCCCGGGG CGGCGAGGCT GGCGCCCACG GCCGCGCAGC CAGCCATCAG CCGGCCCGGG TCCGGCGCGC GGGGCGCGGG CGGCCGCTTG GCGTCCCCGG GCTGCTGAGG CTGCACGCGA CTGGAGTTCA CCATGCGGCC GCTAAATTCA ATTCGCCCTA TAGTGAGTCG TATTACAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTATACG TACGGCAGTT TAAGGTTTAC ACCTATAAAA GAGAGAGCCG TTATCGTCTG TTTGTGGATG TACAGAGTGA TATTATTGAC ACGCCGGGGC GACGGATGGT GATCCCCCTG GCCAGTGCAC GTCTGCTGTC AGATAAAGTC TCCCGTGAAC TTTACCCGGT GGTGCATATC GGGGATGAAA GCTGGCGCAT GATGACCACC GATATGGCCA GTGTGCCGGT CTCCGTTATC GGGGAAGAAG TGGCTGATCT CAGCCACCGC GAAAATGACA TCAAAAACGC CATTAACCTG ATGTTCTGGG GAATATAAAT GTCAGGCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTCACGT AGAAAGCCAG TCCGCAGAAA CGGTGCTGAC CCCGGATGAA TGTCAGCTAC TGGGCTATCT GGACAAGGGA AAACGCAAGC GCAAAGAGAA AGCAGGTAGC TTGCAGTGGG CTTACATGGC GATAGCTAGA CTGGGCGGTT TTATGGACAG CAAGCGAACC GGAATTGCCA GCTGGGGCGC CCTCTGGTAA GGTTGGGAAG CCCTGCAAAG TAAACTGGAT GGCTTTCTTG CCGCCAAGGA TCTGATGGCG CAGGGGATCA AGCTCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AATTATTAAC GCTTACAATT TCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAG
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