pGL4.52[Luc2P STAT5 RE Hygro]
$84.00
In stock
SKU
PL82457
100μg liquid plasmid(Instantly):$105
| name | pGL4.52[Luc2P STAT5 RE Hygro] | another name | apGL4.52[Luc2P STAT5 RE Hygro] |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Hyg | Fluorescent labeling | Fluc |
| Promoter | STAT5 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | RVP3：CTAGCAAAATAGGCTGTCCC | |
| 3′ sequencing primer | GLP4：GGGTAGAATGGCGCTGGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCGTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TACTAGTACG TCTCTCAAGG ATAAGTAAGT AATATTAAGG TACGGGAGGT ATTGGACAGG CCGCAATAAA ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGAATCGAT AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA AACAAACTAG CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC ATTTCTCTGG CCTAACTGGC CGGTACCTGA GCTCAGTTCT GAGAAAAGTA GTTCTGAGAA AAGTAGTTCT GAGAAAAGTA GTTCTGAGAA AAGTAGTTCT GAGAAAAGTC TCGAGGATAT CAAGATCTGG CCTCGGCGGC CAAGCTTAGA CACTAGAGGG TATATAATGG AAGCTCGACT TCCAGCTTGG CAATCCGGTA CTGTTGGTAA AGCCACCATG GAAGATGCCA AAAACATTAA GAAGGGCCCA GCGCCATTCT ACCCACTCGA AGACGGGACC GCCGGCGAGC AGCTGCACAA AGCCATGAAG CGCTACGCCC TGGTGCCCGG CACCATCGCC TTTACCGACG CACATATCGA GGTGGACATT ACCTACGCCG AGTACTTCGA GATGAGCGTT CGGCTGGCAG AAGCTATGAA GCGCTATGGG CTGAATACAA ACCATCGGAT CGTGGTGTGC AGCGAGAATA GCTTGCAGTT CTTCATGCCC GTGTTGGGTG CCCTGTTCAT CGGTGTGGCT GTGGCCCCAG CTAACGACAT CTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATCAGC CAGCCCACCG TCGTATTCGT GAGCAAGAAA GGGCTGCAAA AGATCCTCAA CGTGCAAAAG AAGCTACCGA TCATACAAAA GATCATCATC ATGGATAGCA AGACCGACTA CCAGGGCTTC CAAAGCATGT ACACCTTCGT GACTTCCCAT TTGCCACCCG GCTTCAACGA GTACGACTTC GTGCCCGAGA GCTTCGACCG GGACAAAACC ATCGCCCTGA TCATGAACAG TAGTGGCAGT ACCGGATTGC CCAAGGGCGT AGCCCTACCG CACCGCACCG CTTGTGTCCG ATTCAGTCAT GCCCGCGACC CCATCTTCGG CAACCAGATC ATCCCCGACA CCGCTATCCT CAGCGTGGTG CCATTTCACC ACGGCTTCGG CATGTTCACC ACGCTGGGCT ACTTGATCTG CGGCTTTCGG GTCGTGCTCA TGTACCGCTT CGAGGAGGAG CTATTCTTGC GCAGCTTGCA AGACTATAAG ATTCAATCTG CCCTGCTGGT GCCCACACTA TTTAGCTTCT TCGCTAAGAG CACTCTCATC GACAAGTACG ACCTAAGCAA CTTGCACGAG ATCGCCAGCG GCGGGGCGCC GCTCAGCAAG GAGGTAGGTG AGGCCGTGGC CAAACGCTTC CACCTACCAG GCATCCGCCA GGGCTACGGC CTGACAGAAA CAACCAGCGC CATTCTGATC ACCCCCGAAG GGGACGACAA GCCTGGCGCA GTAGGCAAGG TGGTGCCCTT CTTCGAGGCT AAGGTGGTGG ACTTGGACAC CGGTAAGACA CTGGGTGTGA ACCAGCGCGG CGAGCTGTGC GTCCGTGGCC CCATGATCAT GAGCGGCTAC GTTAACAACC CCGAGGCTAC AAACGCTCTC ATCGACAAGG ACGGCTGGCT GCACAGCGGC GACATCGCCT ACTGGGACGA GGACGAGCAC TTCTTCATCG TGGACCGGCT GAAGAGCCTG ATCAAATACA AGGGCTACCA GGTAGCCCCA GCCGAACTGG AGAGCATCCT GCTGCAACAC CCCAACATCT TCGACGCCGG GGTCGCCGGC CTGCCCGACG ACGATGCCGG CGAGCTGCCC GCCGCAGTCG TCGTGCTGGA ACACGGTAAA ACCATGACCG AGAAGGAGAT CGTGGACTAT GTGGCCAGCC AGGTTACAAC CGCCAAGAAG CTGCGCGGTG GTGTTGTGTT CGTGGACGAG GTGCCTAAAG GACTGACCGG CAAGTTGGAC GCCCGCAAGA TCCGCGAGAT TCTCATTAAG GCCAAGAAGG GCGGCAAGAT CGCCGTGAAT TCTCACGGCT TCCCTCCCGA GGTGGAGGAG CAGGCCGCCG GCACCCTGCC CATGAGCTGC GCCCAGGAGA GCGGCATGGA TAGACACCCT GCTGCTTGCG CCAGCGCCAG GATCAACGTC TAAGGCCGCG ACTCTAGAGT CGGGGCGGCC GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG ATAAGGATCC GTTTGCGTAT TGGGCGCTCT TCCGCTGATC TGCGCAGCAC CATGGCCTGA AATAACCTCT GAAAGAGGAA CTTGGTTAGC TACCTTCTGA GGCGGAAAGA ACCAGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC GATTCTTCTG ACACTAGCGC CACCATGAAG AAGCCCGAAC TCACCGCTAC CAGCGTTGAA AAATTTCTCA TCGAGAAGTT CGACAGTGTG AGCGACCTGA TGCAGTTGTC GGAGGGCGAA GAGAGCCGAG CCTTCAGCTT CGATGTCGGC GGACGCGGCT ATGTACTGCG GGTGAATAGC TGCGCTGATG GCTTCTACAA AGACCGCTAC GTGTACCGCC ACTTCGCCAG CGCTGCACTA CCCATCCCCG AAGTGTTGGA CATCGGCGAG TTCAGCGAGA GCCTGACATA CTGCATCAGT AGACGCGCCC AAGGCGTTAC TCTCCAAGAC CTCCCCGAAA CAGAGCTGCC TGCTGTGTTA CAGCCTGTCG CCGAAGCTAT GGATGCTATT GCCGCCGCCG ACCTCAGTCA AACCAGCGGC TTCGGCCCAT TCGGGCCCCA AGGCATCGGC CAGTACACAA CCTGGCGGGA TTTCATTTGC GCCATTGCTG ATCCCCATGT CTACCACTGG CAGACCGTGA TGGACGACAC CGTGTCCGCC AGCGTAGCTC AAGCCCTGGA CGAACTGATG CTGTGGGCCG AAGACTGTCC CGAGGTGCGC CACCTCGTCC ATGCCGACTT CGGCAGCAAC AACGTCCTGA CCGACAACGG CCGCATCACC GCCGTAATCG ACTGGTCCGA AGCTATGTTC GGGGACAGTC AGTACGAGGT GGCCAACATC TTCTTCTGGC GGCCCTGGCT GGCTTGCATG GAGCAGCAGA CTCGCTACTT CGAGCGCCGG CATCCCGAGC TGGCCGGCAG CCCTCGTCTG CGAGCCTACA TGCTGCGCAT CGGCCTGGAT CAGCTCTACC AGAGCCTCGT GGACGGCAAC TTCGACGATG CTGCCTGGGC TCAAGGCCGC TGCGATGCCA TCGTCCGCAG CGGGGCCGGC ACCGTCGGTC GCACACAAAT CGCTCGCCGG AGCGCAGCCG TATGGACCGA CGGCTGCGTC GAGGTGCTGG CCGACAGCGG CAACCGCCGG CCCAGTACAC GACCGCGCGC TAAGGAGGTA GGTCGAGTTT AAACTCTAGA ACCGGTCATG GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGTTCGAA CTAGATGCTG TCGACCGATG CCCTTGAGAG CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC GCCGCACTTA TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGC GGCCGCAAAT GCTAAACCAC TGCAGTGGTT ACCAGTGCTT GATCAGTGAG GCACCGATCT CAGCGATCTG CCTATTTCGT TCGTCCATAG TGGCCTGACT CCCCGTCGTG TAGATCACTA CGATTCGTGA GGGCTTACCA TCAGGCCCCA GCGCAGCAAT GATGCCGCGA GAGCCGCGTT CACCGGCCCC CGATTTGTCA GCAATGAACC AGCCAGCAGG GAGGGCCGAG CGAAGAAGTG GTCCTGCTAC TTTGTCCGCC TCCATCCAGT CTATGAGCTG CTGTCGTGAT GCTAGAGTAA GAAGTTCGCC AGTGAGTAGT TTCCGAAGAG TTGTGGCCAT TGCTACTGGC ATCGTGGTAT CACGCTCGTC GTTCGGTATG GCTTCGTTCA ACTCTGGTTC CCAGCGGTCA AGCCGGGTCA CATGATCACC CATATTATGA AGAAATGCAG TCAGCTCCTT AGGGCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCGGTG TTGTCGCTCA TGGTAATGGC AGCACTACAC AATTCTCTTA CCGTCATGCC ATCCGTAAGA TGCTTTTCCG TGACCGGCGA GTACTCAACC AAGTCGTTTT GTGAGTAGTG TATACGGCGA CCAAGCTGCT CTTGCCCGGC GTCTATACGG GACAACACCG CGCCACATAG CAGTACTTTG AAAGTGCTCA TCATCGGGAA TCGTTCTTCG GGGCGGAAAG ACTCAAGGAT CTTGCCGCTA TTGAGATCCA GTTCGATATA GCCCACTCTT GCACCCAGTT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCGGGGTG TGCAAAAACA GGCAAGCAAA ATGCCGCAAA GAAGGGAATG AGTGCGACAC GAAAATGTTG GATGCTCAT
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