pGWB406
$99.00
In stock
SKU
PL82451
100μg liquid plasmid(Instantly):$120
| name | pGWB406 | another name | apGWB406 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF,GateWay |
| Fragment species | Empty vector | Prokaryotic resistance | Chl |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | 35s | Replicon | pVS1 |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACTGAA GGCGGGAAAC GACAATCTGA TCCAAGCTCA AGCTGCTCTA GCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTTGCAT GCCTGCAGGT CCCCAGATTA GCCTTTTCAA TTTCAGAAAG AATGCTAACC CACAGATGGT TAGAGAGGCT TACGCAGCAG GTCTCATCAA GACGATCTAC CCGAGCAATA ATCTCCAGGA AATCAAATAC CTTCCCAAGA AGGTTAAAGA TGCAGTCAAA AGATTCAGGA CTAACTGCAT CAAGAACACA GAGAAAGATA TATTTCTCAA GATCAGAAGT ACTATTCCAG TATGGACGAT TCAAGGCTTG CTTCACAAAC CAAGGCAAGT AATAGAGATT GGAGTCTCTA AAAAGGTAGT TCCCACTGAA TCAAAGGCCA TGGAGTCAAA GATTCAAATA GAGGACCTAA CAGAACTCGC CGTAAAGACT GGCGAACAGT TCATACAGAG TCTCTTACGA CTCAATGACA AGAAGAAAAT CTTCGTCAAC ATGGTGGAGC ACGACACACT TGTCTACTCC AAAAATATCA AAGATACAGT CTCAGAAGAC CAAAGGGCAA TTGAGACTTT TCAACAAAGG GTAATATCCG GAAACCTCCT CGGATTCCAT TGCCCAGCTA TCTGTCACTT TATTGTGAAG ATAGTGGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT GCGATAAAGG AAAGGCCATC GTTGAAGATG CCTCTGCCGA CAGTGGTCCC AAAGATGGAC CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGACGTTCC AACCACGTCT TCAAAGCAAG TGGATTGATG TGATATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGAACACGG GGGACTCTAG AGGATCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTGAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCT TCACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CACGGCATGG ACGAGCTGTA CATCACAAGT TTGTACAAAA AAGCTGAACG AGAAACGTAA AATGATATAA ATATCAATAT ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC AACATATCCA GTCACTATGG CGGCCCCTCG AGGGGCCGCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATAATGTGTG GATTTTGAGT TAGGATCCGG CGAGATTTTC AGGAGCTAAG GAAGCTAAAA TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT CAGCTGGATA TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA GTTTTATCCG GCCTTTATTC ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTCCG TATGGCAATG AAAGACGGTG AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT TTTCCATGAG CAAACTGAAA CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATTTCCG GCAGTTTCTA CACATATATT CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGCCTATTT CCCTAAAGGG TTTATTGAGA ATATGTTTTT CGTCTCAGCC AATCCCTGGG TGAGTTTCAC CAGTTTTGAT TTAAACGTGG CCAATATGGA CAACTTCTTC GCCCCCGTTT TCACCATGGG CAAATATTAT ACGCAAGGCG ACAAGGTGCT GATGCCGCTG GCGATTCAGG TTCATCATGC CGTCTGTGAT GGCTTCCATG TCGGCAGAAT GCTTAATGAA TTACAACAGT ACTGCGATGA GTGGCAGGGC GGGGCGTAAA CGCGTGGATC CGGCTTACTA AAAGCCAGAT AACAGTATGC GTATTTGCGC GCTGATTTTT GCGGTATAAG AATATATACT GATATGTATA CCCGAAGTAT GTCAAAAAGA GGTGTGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTCT GGTAAGCACA ACCATGCAGA ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCTGA CGAGAACAGG GACTGGTGAA ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT CTGCTGTCAG ATAAAGTCTC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC AGCTTTCTTG TACAAAGTGG TGATGTACAA GTAAAGCGGC CCGAGCTCGA ATTTCCCCGA TCGTTCAAAC ATTTGGCAAT AAAGTTTCTT AAGATTGAAT CCTGTTGCCG GTCTTGCGAT GATTATCATA TAATTTCTGT TGAATTACGT TAAGCATGTA ATAATTAACA TGTAATGCAT GACGTTATTT ATGAGATGGG TTTTTATGAT TAGAGTCCCG CAATTATACA TTTAATACGC GATAGAAAAC AAAATATAGC GCGCAAACTA GGATAAATTA TCGCGCGCGG TGTCATCTAT GTTACTAGAT CGGGAATTGG TTCCGGAACC AATTCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAG GCTGAATTAG GCGCGCCTAT TTCTGAATTC CCGATCTAGT AACATAGATG ACACCGCGCG CGATAATTTA TCCTAGTTTG CGCGCTATAT TTTGTTTTCT ATCGCGTATT AAATGTATAA TTGCGGGACT CTAATCATAA AAACCCATCT CATAAATAAC GTCATGCATT ACATGTTAAT TATTACATGC TTAACGTAAT TCAACAGAAA TTATATGATA ATCATCGCAA GACCGGCAAC AGGATTCAAT CTTAAGAAAC TTTATTGCCA AATGTTTGAA CGATCGGGGA TCATCCGGGT CTGTGGCGGG AACTCCACGA AAATATCCGA ACGCAGCAAG ATATCGCGGT GCATCTCGGT CTTGCCTGGG CAGTCGCCGC CGACGCCGTT GATGTGGACG CCGGGCCCGA TCATATTGTC GCTCAGGATC GTGGCGTTGT GCTTGTCGGC CGTTGCTGTC GTAATGATAT CGGCACCTTC GACCGCCTGT TCCGCAGAGA TCCCGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GGTCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCATCGCCG TCGGGCATGC GCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCCTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CAGATCCGGT GCAGATTATT TGGATTGAGA GTGAATATGA GACTCTAATT GGATACCGAG GGGAATTTAT GGAACGTCAG TGGAGCATTT TTGACAAGAA ATATTTGCTA GCTGATAGTG ACCTTAGGCG ACTTTTGAAC GCGCAATAAT GGTTTCTGAC GTATGTGCTT AGCTCATTAA ACTCCAGAAA CCCGCGGCTG AGTGGCTCCT TCAACGTTGC GGTTCTGTCA GTTCCAAACG TAAAACGGCT TGTCCCGCGT CATCGGCGGG GGTCATAACG TGACTCCCTT AATTCTCCGC TCATGATGAA TTCAGAAATA AATTCAGCCT AATTCGGCGT TAATTCAGTA CATTAAAAAC GTCCGCAATG TGTTATTAAG TTGTCTAAGC GTCAATTTGT TTACACCACA ATATATCCTG CCACCAGCCA GCCAACAGCT CCCCGACCGG CAGCTCGGCA CAAAATCACC ACTCGATACA GGCAGCCCAT CAGTCCGGGA CGGCGTCAGC GGGAGAGCCG TTGTAAGGCG GCAGACTTTG CTCATGTTAC CGATGCTATT CGGAAGAACG GCAACTAAGC TGCCGGGTTT GAAACACGGA TGATCTCGCG GAGGGTAGCA TGTTGATTGT AACGATGACA GAGCGTTGCT GCCTGTGATC AATTCGGGCA CGAACCCAGT GGACATAAGC CTGTTCGGTT CGTAAGCTGT AATGCAAGTA GCGTATGCGC TCACGCAACT GGTCCAGAAC CTTGACCGAA CGCAGCGGTG GTAACGGCGC AGTGGCGGTT TTCATGGCTT GTTATGACTG TTTTTTTGGG GTACAGTCTA TGCCTCGGGC ATCCAAGCAG CAAGCGCGTT ACGCCGTGGG TCGATGTTTG ATGTTATGGA GCAGCAACGA TGTTACGCAG CAGGGCAGTC GCCCTAAAAC AAAGTTAAAC ATCATGGGGG AAGCGGTGAT CGCCGAAGTA TCGACTCAAC TATCAGAGGT AGTTGGCGTC ATCGAGCGCC ATCTCGAACC GACGTTGCTG GCCGTACATT TGTACGGCTC CGCAGTGGAT GGCGGCCTGA AGCCACACAG TGATATTGAT TTGCTGGTTA CGGTGACCGT AAGGCTTGAT GAAACAACGC GGCGAGCTTT GATCAACGAC CTTTTGGAAA CTTCGGCTTC CCCTGGAGAG AGCGAGATTC TCCGCGCTGT AGAAGTCACC ATTGTTGTGC ACGACGACAT CATTCCGTGG CGTTATCCAG CTAAGCGCGA ACTGCAATTT GGAGAATGGC AGCGCAATGA CATTCTTGCA GGTATCTTCG AGCCAGCCAC GATCGACATT GATCTGGCTA TCTTGCTGAC AAAAGCAAGA GAACATAGCG TTGCCTTGGT AGGTCCAGCG GCGGAGGAAC TCTTTGATCC GGTTCCTGAA CAGGATCTAT TTGAGGCGCT AAATGAAACC TTAACGCTAT GGAACTCGCC GCCCGACTGG GCTGGCGATG AGCGAAATGT AGTGCTTACG TTGTCCCGCA TTTGGTACAG CGCAGTAACC GGCAAAATCG CGCCGAAGGA TGTCGCTGCC GACTGGGCAA TGGAGCGCCT GCCGGCCCAG TATCAGCCCG TCATACTTGA AGCTAGACAG GCTTATCTTG GACAAGAAGA AGATCGCTTG GCCTCGCGCG CAGATCAGTT GGAAGAATTT GTCCACTACG TGAAAGGCGA GATCACCAAG GTAGTCGGCA AATAATGTCT AGCTAGAAAT TCGTTCAAGC CGACGCCGCT TCGCGGCGCG GCTTAACTCA AGCGTTAGAT GCACTAAGCA CATAATTGCT CACAGCCAAA CTATCAGGTC AAGTCTGCTT TTATTATTTT TAAGCGTGCA TAATAAGCCC TACACAAATT GGGAGATATA TCATGCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGGGTG CCTTGATGTG GGCGCCGGCG GTCGAGTGGC GACGGCGCGG CTTGTCCGCG CCCTGGTAGA TTGCCTGGCC GTAGGCCAGC CATTTTTGAG CGGCCAGCGG CCGCGATAGG CCGACGCGAA GCGGCGGGGC GTAGGGAGCG CAGCGACCGA AGGGTAGGCG CTTTTTGCAG CTCTTCGGCT GTGCGCTGGC CAGACAGTTA TGCACAGGCC AGGCGGGTTT TAAGAGTTTT AATAAGTTTT AAAGAGTTTT AGGCGGAAAA ATCGCCTTTT TTCTCTTTTA TATCAGTCAC TTACATGTGT GACCGGTTCC CAATGTACGG CTTTGGGTTC CCAATGTACG GGTTCCGGTT CCCAATGTAC GGCTTTGGGT TCCCAATGTA CGTGCTATCC ACAGGAAAGA GACCTTTTCG ACCTTTTTCC CCTGCTAGGG CAATTTGCCC TAGCATCTGC TCCGTACATT AGGAACCGGC GGATGCTTCG CCCTCGATCA GGTTGCGGTA GCGCATGACT AGGATCGGGC CAGCCTGCCC CGCCTCCTCC TTCAAATCGT ACTCCGGCAG GTCATTTGAC CCGATCAGCT TGCGCACGGT GAAACAGAAC TTCTTGAACT CTCCGGCGCT GCCACTGCGT TCGTAGATCG TCTTGAACAA CCATCTGGCT TCTGCCTTGC CTGCGGCGCG GCGTGCCAGG CGGTAGAGAA AACGGCCGAT GCCGGGATCG ATCAAAAAGT AATCGGGGTG AACCGTCAGC ACGTCCGGGT TCTTGCCTTC TGTGATCTCG CGGTACATCC AATCAGCTAG CTCGATCTCG ATGTACTCCG GCCGCCCGGT TTCGCTCTTT ACGATCTTGT AGCGGCTAAT CAAGGCTTCA CCCTCGGATA CCGTCACCAG GCGGCCGTTC TTGGCCTTCT TCGTACGCTG CATGGCAACG TGCGTGGTGT TTAACCGAAT GCAGGTTTCT ACCAGGTCGT CTTTCTGCTT TCCGCCATCG GCTCGCCGGC AGAACTTGAG TACGTCCGCA ACGTGTGGAC GGAACACGCG GCCGGGCTTG TCTCCCTTCC CTTCCCGGTA TCGGTTCATG GATTCGGTTA GATGGGAAAC CGCCATCAGT ACCAGGTCGT AATCCCACAC ACTGGCCATG CCGGCCGGCC CTGCGGAAAC CTCTACGTGC CCGTCTGGAA GCTCGTAGCG GATCACCTCG CCAGCTCGTC GGTCACGCTT CGACAGACGG AAAACGGCCA CGTCCATGAT GCTGCGACTA TCGCGGGTGC CCACGTCATA GAGCATCGGA ACGAAAAAAT CTGGTTGCTC GTCGCCCTTG GGCGGCTTCC TAATCGACGG CGCACCGGCT GCCGGCGGTT GCCGGGATTC TTTGCGGATT CGATCAGCGG CCGCTTGCCA CGATTCACCG GGGCGTGCTT CTGCCTCGAT GCGTTGCCGC TGGGCGGCCT GCGCGGCCTT CAACTTCTCC ACCAGGTCAT CACCCAGCGC CGCGCCGATT TGTACCGGGC CGGATGGTTT GCGACCGCTC ACGCCGATTC CTCGGGCTTG GGGGTTCCAG TGCCATTGCA GGGCCGGCAG ACAACCCAGC CGCTTACGCC TGGCCAACCG CCCGTTCCTC CACACATGGG GCATTCCACG GCGTCGGTGC CTGGTTGTTC TTGATTTTCC ATGCCGCCTC CTTTAGCCGC TAAAATTCAT CTACTCATTT ATTCATTTGC TCATTTACTC TGGTAGCTGC GCGATGTATT CAGATAGCAG CTCGGTAATG GTCTTGCCTT GGCGTACCGC GTACATCTTC AGCTTGGTGT GATCCTCCGC CGGCAACTGA AAGTTGACCC GCTTCATGGC TGGCGTGTCT GCCAGGCTGG CCAACGTTGC AGCCTTGCTG CTGCGTGCGC TCGGACGGCC GGCACTTAGC GTGTTTGTGC TTTTGCTCAT TTTCTCTTTA CCTCATTAAC TCAAATGAGT TTTGATTTAA TTTCAGCGGC CAGCGCCTGG ACCTCGCGGG CAGCGTCGCC CTCGGGTTCT GATTCAAGAA CGGTTGTGCC GGCGGCGGCA GTGCCTGGGT AGCTCACGCG CTGCGTGATA CGGGACTCAA GAATGGGCAG CTCGTACCCG GCCAGCGCCT CGGCAACCTC ACCGCCGATG CGCGTGCCTT TGATCGCCCG CGACACGACA AAGGCCGCTT GTAGCCTTCC ATCCGTGACC TCAATGCGCT GCTTAACCAG CTCCACCAGG TCGGCGGTGG CCCATATGTC GTAAGGGCTT GGCTGCACCG GAATCAGCAC GAAGTCGGCT GCCTTGATCG CGGACACAGC CAAGTCCGCC GCCTGGGGCG CTCCGTCGAT CACTACGAAG TCGCGCCGGC CGATGGCCTT CACGTCGCGG TCAATCGTCG GGCGGTCGAT GCCGACAACG GTTAGCGGTT GATCTTCCCG CACGGCCGCC CAATCGCGGG CACTGCCCTG GGGATCGGAA TCGACTAACA GAACATCGGC CCCGGCGAGT TGCAGGGCGC GGGCTAGATG GGTTGCGATG GTCGTCTTGC CTGACCCGCC TTTCTGGTTA AGTACAGCGA TAACCTTCAT GCGTTCCCCT TGCGTATTTG TTTATTTACT CATCGCATCA TATACGCAGC GACCGCATGA CGCAAGCTGT TTTACTCAAA TACACATCAC CTTTTTAGAC GGCGGCGCTC GGTTTCTTCA GCGGCCAAGC TGGCCGGCCA GGCCGCCAGC TTGGCATCAG ACAAACCGGC CAGGATTTCA TGCAGCCGCA CGGTTGAGAC GTGCGCGGGC GGCTCGAACA CGTACCCGGC CGCGATCATC TCCGCCTCGA TCTCTTCGGT AATGAAAAAC GGTTCGTCCT GGCCGTCCTG GTGCGGTTTC ATGCTTGTTC CTCTTGGCGT TCATTCTCGG CGGCCGCCAG GGCGTCGGCC TCGGTCAATG CGTCCTCACG GAAGGCACCG CGCCGCCTGG CCTCGGTGGG CGTCACTTCC TCGCTGCGCT CAAGTGCGCG GTACAGGGTC GAGCGATGCA CGCCAAGCAG TGCAGCCGCC TCTTTCACGG TGCGGCCTTC CTGGTCGATC AGCTCGCGGG CGTGCGCGAT CTGTGCCGGG GTGAGGGTAG GGCGGGGGCC AAACTTCACG CCTCGGGCCT TGGCGGCCTC GCGCCCGCTC CGGGTGCGGT CGATGATTAG GGAACGCTCG AACTCGGCAA TGCCGGCGAA CACGGTCAAC ACCATGCGGC CGGCCGGCGT GGTGGTGTCG GCCCACGGCT CTGCCAGGCT ACGCAGGCCC GCGCCGGCCT CCTGGATGCG CTCGGCAATG TCCAGTAGGT CGCGGGTGCT GCGGGCCAGG CGGTCTAGCC TGGTCACTGT CACAACGTCG CCAGGGCGTA GGTGGTCAAG CATCCTGGCC AGCTCCGGGC GGTCGCGCCT GGTGCCGGTG ATCTTCTCGG AAAACAGCTT GGTGCAGCCG GCCGCGTGCA GTTCGGCCCG TTGGTTGGTC AAGTCCTGGT CGTCGGTGCT GACGCGGGCA TAGCCCAGCA GGCCAGCGGC GGCGCTCTTG TTCATGGCGT AATGTCTCCG GTTCTAGTCG CAAGTATTCT ACTTTATGCG ACTAAAACAC GCGACAAGAA AACGCCAGGA AAAGGGCAGG GCGGCAGCCT GTCGCGTAAC TTAGGACTTG TGCGACATGT CGTTTTCAGA AGACGGCTGC ACTGAACGTC AGAAGCCGAC TGCACTATAG CAGCGGAGGG GTTGGATCAA AGTACTTTGA TCCCGAGGGG AACCCTGTGG TTGGCATGCA CATACAAATG GACGAACGGA TAAACCT
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