pUC18-TMV
$113.00
In stock
SKU
PL82448
100μg liquid plasmid(Instantly):$134
| name | pUC18-TMV | another name | apUC18-TMV |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Virus | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTTAATACG ACTCACTATA GTATTTTTAC AACAATTACC AACAACAACA AACAACAAAC AACATTACAA TTACTATTTA CAATTACAAT GGCATACACA CAGACAGCTA CCACATCAGC TTTGCTGGAC ACTGTCCGAG GAAACAACTC CTTGGTCAAT GATCTAGCAA AGCGTCGTCT TTACGACACA GCGGTTGAAG AGTTTAACGC TCGTGACCGC AGGCCCAAGG TGAACTTTTC AAAAGTAATA AGCGAGGAGC AGACGCTTAT TGCTACCCGG GCGTATCCAG AATTCCAAAT TACATTTTAT AACACGCAAA ATGCCGTGCA TTCGCTTGCA GGTGGATTGC GATCTTTAGA ACTGGAATAT CTGATGATGC AAATTCCCTA CGGATCATTG ACTTATGACA TAGGCGGGAA TTTTGCATCG CATCTGTTCA AGGGACGAGC ATATGTACAC TGCTGCATGC CCAACCTGGA CGTTCGAGAC ATCATGCGGC ACGAAGGCCA GAAAGACAGT ATTGAACTAT ACCTTTCTAG GCTAGAGAGA GGGGGGAAAA CAGTCCCCAA CTTCCAAAAG GAAGCATTTG ACAGATACGC AGAAATTCCT GAAGACGCTG TCTGTCACAA TACTTTCCAG ACAATGCGAC ATCAGCCGAT GCAGCAATCA GGCAGAGTGT ATGCCATTGC GCTACACAGC ATATATGACA TACCAGCCGA TGAGTTCGGG GCGGCACTCT TGAGGAAAAA TGTCCATACG TGCTATGCCG CTTTCCACTT CTCTGAGAAC CTGCTTCTTG AAGATTCATA CGTCAATTTG GACGAAATCA ACGCGTGTTT TTCGCGCGAT GGAGACAAGT TGACCTTTTC TTTTGCATCA GAGAGTACTC TTAATTATTG TCATAGTTAT TCTAATATTC TTAAGTATGT GTGCAAAACT TACTTCCCGG CCTCTAATAG AGAGGTTTAC ATGAAGGAGT TTTTAGTCAC CAGAGTTAAT ACCTGGTTTT GTAAGTTTTC TAGAATAGAT ACTTTTCTTT TGTACAAAGG TGTGGCCCAT AAAAGTGTAG ATAGTGAGCA GTTTTATACT GCAATGGAAG ACGCATGGCA TTACAAAAAG ACTCTTGCAA TGTGCAACAG CGAGAGAATC CTCCTTGAGG ATTCATCATC AGTCAATTAC TGGTTTCCCA AAATGAGGGA TATGGTCATC GTACCATTAT TCGACATTTC TTTGGAGACT AGTAAGAGGA CGCGCAAGGA AGTCTTAGTG TCCAAGGATT TCGTGTTTAC AGTGCTTAAC CACATTCGAA CATACCAGGC GAAAGCTCTT ACATACGCAA ATGTTTTGTC CTTTGTCGAA TCGATTCGAT CGAGGGTAAT CATTAACGGT GTGACAGCGA GGTCCGAATG GGATGTGGAC AAATCTTTGT TACAATCCTT GTCCATGACG TTTTACCTGC ATACTAAGCT TGCCGTTCTA AAGGATGACT TACTGATTAG CAAGTTTAGT CTCGGTTCGA AAACGGTGTG CCAGCATGTG TGGGATGAGA TTTCGCTGGC GTTTGGGAAC GCATTTCCCT CCGTGAAAGA GAGGCTCTTG AACAGGAAAC TTATCAGAGT GGCAGGCGAC GCATTAGAGA TCAGGGTGCC TGATCTATAT GTGACCTTCC ACGACAGATT AGTGACTGAG TACAAGGCCT CTGTGGACAT GCCTGCGCTT GACATTAGGA AGAAGATGGA AGAAACGGAA GTGATGTACA ATGCACTTTC AGAGTTATCG GTGTTAAGGG AGTCTGACAA ATTCGATGTT GATGTTTTTT CCCAGATGTG CCAATCTTTG GAAGTTGACC CAATGACGGC AGCGAAGGTT ATAGTCGCGG TCATGAGCAA TGAGAGCGGT CTGACTCTCA CATTTGAACG ACCTACTGAG GCGAATGTTG CGCTAGCTTT ACAGGATCAA GAGAAGGCTT CAGAAGGTGC TTTGGTAGTT ACCTCAAGAG AAGTTGAAGA ACCGTCCATG AAGGGTTCGA TGGCCAGAGG AGAGTTACAA TTAGCTGGTC TTGCTGGAGA TCATCCGGAG TCGTCCTATT CTAAGAACGA GGAGATAGAG TCTTTAGAGC AGTTTCATAT GGCAACGGCA GATTCGTTAA TTCGTAAGCA GATGAGCTCG ATTGTGTACA CGGGTCCGAT TAAAGTTCAG CAAATGAAAA ACTTTATCGA TAGCCTGGTA GCATCACTAT CTGCTGCGGT GTCGAATCTC GTCAAGATCC TCAAAGATAC AGCTGCTATT GACCTTGAAA CCCGTCAAAA GTTTGGAGTC TTGGATGTTG CATCTAGGAA GTGGTTAATC AAACCAACGG CCAAGAGTCA TGCATGGGGT GTTGTTGAAA CCCACGCGAG GAAGTATCAT GTGGCGCTTT TGGAATATGA TGAGCAGGGT GTGGTGACAT GCGATGATTG GAGAAGAGTA GCTGTCAGCT CTGAGTCTGT TGTTTATTCC GACATGGCGA AACTCAGAAC TCTGCGCAGA CTGCTTCGAA ACGGAGAACC GCATGTCAGT AGCGCAAAGG TTGTTCTTGT GGACGGAGTT CCGGGCTGTG GGAAAACCAA AGAAATTCTT TCCAGGGTTA ATTTTGATGA AGATCTAATT TTAGTACCTG GGAAGCAAGC CGCGGAAATG ATCAGAAGAC GTGCGAATTC CTCAGGGATT ATTGTGGCCA CGAAGGACAA CGTTAAAACC GTTGATTCTT TCATGATGAA TTTTGGGAAA AGCACACGCT GTCAGTTCAA GAGGTTATTC ATTGATGAAG GGTTGATGTT GCATACTGGT TGTGTTAATT TTCTTGTGGC GATGTCATTG TGCGAAATTG CATATGTTTA CGGAGACACA CAGCAGATTC CATACATCAA TAGAGTTTCA GGATTCCCGT ACCCCGCCCA TTTTGCCAAA TTGGAAGTTG ACGAGGTGGA GACACGCAGA ACTACTCTCC GTTGTCCAGC CGATGTCACA CATTATCTGA ACAGGAGATA TGAGGGCTTT GTCATGAGCA CTTCTTCGGT TAAAAAGTCT GTTTCGCAGG AGATGGTCGG CGGAGCCGCC GTGATCAATC CGATCTCAAA ACCCTTGCAT GGCAAGATCC TGACTTTTAC CCAATCGGAT AAAGAAGCTC TGCTTTCAAG AGGGTATTCA GATGTTCACA CTGTGCATGA AGTGCAAGGC GAGACATACT CTGATGTTTC ACTAGTTAGG TTAACCCCTA CACCAGTCTC CATCATTGCA GGAGACAGCC CACATGTTTT GGTCGCATTG TCAAGGCACA CCTGTTCGCT CAAGTACTAC ACTGTTGTTA TGGATCCTTT AGTTAGTATC ATTAGAGATC TAGAGAAACT TAGCTCGTAC TTGTTAGATA TGTATAAGGT CGATGCAGGA ACACAATAGC AATTACAGAT TGACTCGGTG TTCAAAGGTT CCAATCTTTT TGTTGCAGCG CCAAAGACTG GTGATATTTC TGATATGCAG TTTTACTATG ATAAGTGTCT CCCAGGCAAC AGCACCATGA TGAATAATTT TGATGCTGTT ACCATGAGGT TGACTGACAT TTCATTGAAT GTCAAAGATT GCATATTGGA TATGTCTAAG TCTGTTGCTG CGCCTAAGGA TCAAATCAAA CCACTAATAC CTATGGTACG AACGGCGGCA GAAATGCCAC GCCAGACTGG ACTATTGGAA AATTTAGTGG CGATGATTAA AAGGAACTTT AACGCACCCG AGTTGTCTGG CATCATTGAT ATTGAAAATA CTGCATCTTT AGTTGTAGAT AAGTTTTTTG ATAGTTATTT GCTTAAAGAA AAAAGAAAAC CAAATAAAAA TGTTTCTTTG TTCAGTAGAG AGTCTCTCAA TAGATGGTTA GAAAAGCAGG AACAGGTAAC AATAGGCCAG CTCGCAGATT TTGATTTTGT AGATTTGCCA GCAGTTGATC AGTACAGACA CATGATTAAA GCACAACCCA AGCAAAAATT GGACACTTCA ATCCAAACGG AGTACCCGGC TTTGCAGACG ATTGTGTACC ATTCAAAAAA GATCAATGCA ATATTTGGCC CGTTGTTTAG TGAGCTTACT AGGCAATTAC TGGACAGTGT TGATTCGAGC AGATTTTTGT TTTTCACAAG AAAGACACCA GCGCAGATTG AGGATTTCTT CGGAGATCTC GACAGTCATG TGCCGATGGA TGTCTTGGAG CTGGATATAT CAAAATACGA CAAATCTCAG AATGAATTCC ACTGTGCAGT AGAATACGAG ATCTGGCGAA GATTGGGTTT TGAAGACTTC TTGGGAGAAG TTTGGAAACA AGGGCATAGA AAGACCACCC TCAAGGATTA TACCGCAGGT ATAAAAACTT GCATCTGGTA TCAAAGAAAG AGCGGGGACG TCACGACGTT CATTGGAAAC ACTGTGATCA TTGCTGCATG TTTGGCCTCG ATGCTTCCGA TGGAGAAAAT AATCAAAGGA GCCTTTTGCG GTGACGATAG TCTGCTGTAC TTTCCAAAGG GTTGTGAGTT TCCGGATGTG CAACACTCCG CGAATCTTAT GTGGAATTTT GAAGCAAAAC TGTTTAAAAA ACAGTATGGA TACTTTTGCG GAAGATATGT AATACATCAC GACAGAGGAT GCATTGTGTA TTACGATCCC CTAAAGTTGA TCTCGAAACT TGGTGCTAAA CACATCAAGG ATTGGGAACA CTTGGAGGAG TTCAGAAGGT CTCTTTGTGA TGTTGCTGTT TCGTTGAACA ATTGTGCGTA TTACACACAG TTGGACGACG CTGTATGGGA GGTTCATAAG ACCGCCCCTC CAGGTTCGTT TGTTTATAAA AGTCTGGTGA AGTATTTGTC TGATAAAGTT CTTTTTAGAA GTTTGTTTAT AGATGGCTCT AGTTGTTAAA GGAAAAGTGA ATATCAATGA GTTTATCGAC CTGACAAAAA TGGAGAAGAT CTTACCGTCG ATGTTTACCC CTGTAAAGAG TGTTATGTGT TCCAAAGTTG ATAAAATAAT GGTTCATGAG AATGAGTCAT TGTCAGAGGT GAACCTTCTT AAAGGAGTTA AGCTTATTGA TAGTGGATAC GTCTGTTTAG CCGGTTTGGT CGTCACGGGC GAGTGGAACT TGCCTGACAA TTGCAGAGGA GGTGTGAGCG TGTGTCTGGT GGACAAAAGG ATGGAAAGAG CCGACGAGGC CACTCTCGGA TCTTACTACA CAGCAGCTGC AAAGAAAAGA TTTCAGTTCA AGGTCGTTCC CAATTATGCT ATAACCACCC AGGACGCGAT GAAAAACGTC TGGCAAGTTT TAGTTAATAT TAGAAATGTG AAGATGTCAG CGGGTTTCTG TCCGCTTTCT CTGGAGTTTG TGTCGGTGTG TATTGTTTAT AGAAATAATA TAAAATTAGG TTTGAGAGAG AAGATTACAA ACGTGAGAGA CGGAGGGCCC ATGGAACTTA CAGAAGAAGT CGTTGATGAG TTCATGGAAG ATGTCCCTAT GTCGATCAGG CTTGCAAAGT TTCGATCTCG AACCGGAAAA AAGAGTGATG TCCGCAAAGG GAAAAATAGT AGTAATGATC GGTCAGTGCC GAACAAGAAC TATAGAAATG TTAAGGATTT TGGAGGAATG AGTTTTAAAA AGAATAATTT AATCGATGAT GATTCGGAGG CTACTGTCGC CGAATCGGAT TCGTTTTAAA TATGTCTTAC AGTATCACTA CTCCATCTCA GTTCGTGTTC TTGTCATCAG CGTGGGCCGA CCCAATAGAG TTAATTAATT TATGTACTAA TGCCTTAGGA AATCAGTTTC AAACACAACA AGCTCGAACT GTCGTTCAAA GACAATTCAG TGAGGTGTGG AAACCTTCAC CACAAGTAAC TGTTAGGTTC CCTGACAGTG ACTTTAAGGT GTACAGGTAC AATGCGGTAT TAGACCCGCT AGTCACAGCA CTGTTAGGTG CATTCGACAC TAGAAATAGA ATAATAGAAG TTGAAAATCA GGCGAACCCC ACGACTGCCG AAACGTTAGA TGCTACTCGT AGAGTAGACG ACGCAACGGT GGCCATAAGG AGCGCGATAA ATAATTTAAT AGTAGAATTG ATCAGAGGAA CCGGATCTTA TAATCGGAGC TCTTTCGAGA GCTCTTCTGG TTTGGTTTGG ACCTCTGGTC CTGCAACTTG AGGTAGTCAA GATGCATAAT AAATAACGGA TTGTGTCCGT AATCACACGT GGTGCGTACG ATAACGCATA GTGTTTTTCC CTCCACTTAA ATCGAAGGGT TGTGTCTTGG ATCGCGCGGG TCAAATGTAT ATGGTTCATA TACATCCGCA GGCACGTAAT AAAGCGAGGG GTTCGAATCC CCCCGTTACC CCCGGTAGGG GCCCAGGTAC CGAGCTCGAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTC
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