pBBR1MCS2-Tac-mCherry
$128.00
In stock
SKU
PL82428
100μg liquid plasmid(Instantly):$207
| name | pBBR1MCS2-Tac-mCherry | another name | apBBR1MCS2-Tac-mCherry |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | Tac | Replicon | pBBR1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13R： | |
| 3′ sequencing primer | M13F： | Copy number | Extremely low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACCTTCGGGA GCGCCTGAAG CCCGTTCTGG ACGCCCTGGG GCCGTTGAAT CGGGATATGC AGGCCAAGGC CGCCGCGATC ATCAAGGCCG TGGGCGAAAA GCTGCTGACG GAACAGCGGG AAGTCCAGCG CCAGAAACAG GCCCAGCGCC AGCAGGAACG CGGGCGCGCA CATTTCCCCG AAAAGTGCCA CCTGGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG CGATAGCTAG ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC AAGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT GGCGATTCAG GTTCATCATG CCGTTTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA ATTACAACAG TTTTTATGCA TGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG CAATTAACCC TCACTAAAGG GAACAAAAGC TGGGTACCGG GCCCCCCCTC GAGGTCGACG GTATCGATAA GCTTGATATC GAATTCGAGC TCGGTACCTC GCGAATGCAT CTAGATCACA TTCACCACCC TGAATTGACT CTCTTCCGGG CGCTATCATG CCATACCGCG AAAGGTTTTG CGCCATTCGA TGGTGTCCGG GATCTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA ATTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATG TCTCATGAGC GGATATTTCA CACAGGAAAC AGTATTCATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC TCCGAGCGGA TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGCA GAGGCTGAAG CTGAAGGACG GCGGCCACTA CGACGCTGAG GTCAAGACCA CCTACAAGGC CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC CCACAACGAG GACTACACCA TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC CGGCGGCATG GACGAGCTGT ACAAGTAATT AACCTAGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAACCTCA GGCATTTGAG AAGCACACGG TCACACATCG GATCCACTAG TTCTAGAGCG GCCGCCACCG CGGTGGAGCT CCAATTCGCC CTATAGTGAG TCGTATTACG CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA GGCAGTTATT GGTGCCCTTA AACGCCTGGT GCTACGCCTG AATAAGTGAT AATAAGCGGA TGAATGGCAG AAATTCGAAA GCAAATTCGA CCCGGTCGTC GGTTCAGGGC AGGGTCGTTA AATAGCCGCT TATGTCTATT GCTGGTTTAC CGGTTTATTG ACTACCGGAA GCAGTGTGAC CGTGTGCTTC TCAAATGCCT GAGGCCAGTT TGCTCAGGCT CTCCCCGTGG AGGTAATAAT TGACGATATG ATCATTTATT CTGCCTCCCA GAGCCTGATA AAAACGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG AGGCGGCTAC AGCCGATAGT CTGGAACAGC GCACTTACGG GTTGCTGCGC AACCCAAGTG CTACCGGCGC GGCAGCGTGA CCCGTGTCGG CGGCTCCAAC GGCTCGCCAT CGTCCAGAAA ACACGGCTCA TCGGGCATCG GCAGGCGCTG CTGCCCGCGC CGTTCCCATT CCTCCGTTTC GGTCAAGGCT GGCAGGTCTG GTTCCATGCC CGGAATGCCG GGCTGGCTGG GCGGCTCCTC GCCGGGGCCG GTCGGTAGTT GCTGCTCGCC CGGATACAGG GTCGGGATGC GGCGCAGGTC GCCATGCCCC AACAGCGATT CGTCCTGGTC GTCGTGATCA ACCACCACGG CGGCACTGAA CACCGACAGG CGCAACTGGT CGCGGGGCTG GCCCCACGCC ACGCGGTCAT TGACCACGTA GGCCGACACG GTGCCGGGGC CGTTGAGCTT CACGACGGAG ATCCAGCGCT CGGCCACCAA GTCCTTGACT GCGTATTGGA CCGTCCGCAA AGAACGTCCG ATGAGCTTGG AAAGTGTCTT CTGGCTGACC ACCACGGCGT TCTGGTGGCC CATCTGCGCC ACGAGGTGAT GCAGCAGCAT TGCCGCCGTG GGTTTCCTCG CAATAAGCCC GGCCCACGCC TCATGCGCTT TGCGTTCCGT TTGCACCCAG TGACCGGGCT TGTTCTTGGC TTGAATGCCG ATTTCTCTGG ACTGCGTGGC CATGCTTATC TCCATGCGGT AGGGTGCCGC ACGGTTGCGG CACCATGCGC AATCAGCTGC AACTTTTCGG CAGCGCGACA ACAATTATGC GTTGCGTAAA AGTGGCAGTC AATTACAGAT TTTCTTTAAC CTACGCAATG AGCTATTGCG GGGGGTGCCG CAATGAGCTG TTGCGTACCC CCCTTTTTTA AGTTGTTGAT TTTTAAGTCT TTCGCATTTC GCCCTATATC TAGTTCTTTG GTGCCCAAAG AAGGGCACCC CTGCGGGGTT CCCCCACGCC TTCGGCGCGG CTCCCCCTCC GGCAAAAAGT GGCCCCTCCG GGGCTTGTTG ATCGACTGCG CGGCCTTCGG CCTTGCCCAA GGTGGCGCTG CCCCCTTGGA ACCCCCGCAC TCGCCGCCGT GAGGCTCGGG GGGCAGGCGG GCGGGCTTCG CCTTCGACTG CCCCCACTCG CATAGGCTTG GGTCGTTCCA GGCGCGTCAA GGCCAAGCCG CTGCGCGGTC GCTGCGCGAG CCTTGACCCG CCTTCCACTT GGTGTCCAAC CGGCAAGCGA AGCGCGCAGG CCGCAGGCCG GAGGCTTTTC CCCAGAGAAA ATTAAAAAAA TTGATGGGGC AAGGCCGCAG GCCGCGCAGT TGGAGCCGGT GGGTATGTGG TCGAAGGCTG GGTAGCCGGT GGGCAATCCC TGTGGTCAAG CTCGTGGGCA GGCGCAGCCT GTCCATCAGC TTGTCCAGCA GGGTTGTCCA CGGGCCGAGC GAAGCGAGCC AGCCGGTGGC CGCTCGCGGC CATCGTCCAC ATATCCACGG GCTGGCAAGG GAGCGCAGCG ACCGCGCAGG GCGAAGCCCG GAGAGCAAGC CCGTAGGGCG CCGCAGCCGC CGTAGGCGGT CACGACTTTG CGAAGCAAAG TCTAGTGAGT ATACTCAAGC ATTGAGTGGC CCGCCGGAGG CACCGCCTTG CGCTGCCCCC GTCGAGCCGG TTGGACACCA AAAGGGAGGG GCAGGCATGG CGGCATACGC GATCATGCGA TGCAAGAAGC TGGCGAAAAT GGGCAACGTG GCGGCCAGTC TCAAGCACGC CTACCGCGAG CGCGAGACGC CCAACGCTGA CGCCAGCAGG ACGCCAGAGA ACGAGCACTG GGCGGCCAGC AGCACCGATG AAGCGATGGG CCGACTGCGC GAGTTGCTGC CAGAGAAGCG GCGCAAGGAC GCTGTGTTGG CGGTCGAGTA CGTCATGACG GCCAGCCCGG AATGGTGGAA GTCGGCCAGC CAAGAACAGC AGGCGGCGTT CTTCGAGAAG GCGCACAAGT GGCTGGCGGA CAAGTACGGG GCGGATCGCA TCGTGACGGC CAGCATCCAC CGTGACGAAA CCAGCCCGCA CATGACCGCG TTCGTGGTGC CGCTGACGCA GGACGGCAGG CTGTCGGCCA AGGAGTTCAT CGGCAACAAA GCGCAGATGA CCCGCGACCA GACCACGTTT GCGGCCGCTG TGGCCGATCT AGGGCTGCAA CGGGGCATCG AGGGCAGCAA GGCACGTCAC ACGCGCATTC AGGCGTTCTA CGAGGCCCTG GAGCGGCCAC CAGTGGGCCA CGTCACCATC AGCCCGCAAG CGGTCGAGCC ACGCGCCTAT GCACCGCAGG GATTGGCCGA AAAGCTGGGA ATCTCAAAGC GCGTTGAGAC GCCGGAAGCC GTGGCCGACC GGCTGACAAA AGCGGTTCGG CAGGGGTATG AGCCTGCCCT ACAGGCCGCC GCAGGAGCGC GTGAGATGCG CAAGAAGGCC GATCAAGCCC AAGAGACGGC CCGAG
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