pABCA1(human)-2-mut-Fluc-Hyg
$70.00
In stock
SKU
PL82408
100μg liquid plasmid(Instantly):$91
| name | pABCA1(human)-2-mut-Fluc-Hyg | another name | apABCA1(human)-2-mut-Fluc-Hyg |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Hyg | Fluorescent labeling | Fluc |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACATACGCT CTCCATCAAA ACAAAACGAA ACAAAACAAA CTAGCAAAAT AGGCTGTCCC CAGTGCAAGT GCAGGTGCCA GAACATTTCT CTGGCCTAAC TGGCCGGTAC CTTAGTCAAG GTTACCTGGT ATGTCTGGTG GCAAAGCAAA TTCTCTGAAT CACTACACTA CACCGTACTG CCTCACTGGT AAAGTTAATA GTTTGCTCTT TTCTGTTGTG AATGCCCCTG CCAACTTTAC CATGAGTTGA AAGTACTCCA GGAAACATAA GGATTATTGT TCATCAAAAA GCTACTAGAA CAAAGACAGC GGTTTACCTT GACATTATTT CTGGTGTCCA GTCCTGTCAT AAATCTTCCC AAGCTGTTGA GAAATCGATC TGCAGAACTG TCCTGTAAAC AACAGAAACC TGCCTCAGTT GTGACTGTTC ACATAGATAA GGGGCAACAG TCAATCTGGA ACCTGTGGAC AACAGGACGG CCCTGTGAAG AGCAATGCCA CTACCTTGTT CACTGAAATT AAATGTGAAG CCATTAAATA AACGGCTCCT GAGAACCAAG TGACTAGAAA TCTCTCGAGG ATATCAAGAT CTGGCCTCGG CGGCCAAGCT TAGACACTAG AGGGTATATA ATGGAAGCTC GACTTCCAGC TTGGCAATCC GGTACTGTTG GTAAAGCCAC CATGGAAGAT GCCAAAAACA TTAAGAAGGG CCCAGCGCCA TTCTACCCAC TCGAAGACGG GACCGCCGGC GAGCAGCTGC ACAAAGCCAT GAAGCGCTAC GCCCTGGTGC CCGGCACCAT CGCCTTTACC GACGCACATA TCGAGGTGGA CATTACCTAC GCCGAGTACT TCGAGATGAG CGTTCGGCTG GCAGAAGCTA TGAAGCGCTA TGGGCTGAAT ACAAACCATC GGATCGTGGT GTGCAGCGAG AATAGCTTGC AGTTCTTCAT GCCCGTGTTG GGTGCCCTGT TCATCGGTGT GGCTGTGGCC CCAGCTAACG ACATCTACAA CGAGCGCGAG CTGCTGAACA GCATGGGCAT CAGCCAGCCC ACCGTCGTAT TCGTGAGCAA GAAAGGGCTG CAAAAGATCC TCAACGTGCA AAAGAAGCTA CCGATCATAC AAAAGATCAT CATCATGGAT AGCAAGACCG ACTACCAGGG CTTCCAAAGC ATGTACACCT TCGTGACTTC CCATTTGCCA CCCGGCTTCA ACGAGTACGA CTTCGTGCCC GAGAGCTTCG ACCGGGACAA AACCATCGCC CTGATCATGA ACAGTAGTGG CAGTACCGGA TTGCCCAAGG GCGTAGCCCT ACCGCACCGC ACCGCTTGTG TCCGATTCAG TCATGCCCGC GACCCCATCT TCGGCAACCA GATCATCCCC GACACCGCTA TCCTCAGCGT GGTGCCATTT CACCACGGCT TCGGCATGTT CACCACGCTG GGCTACTTGA TCTGCGGCTT TCGGGTCGTG CTCATGTACC GCTTCGAGGA GGAGCTATTC TTGCGCAGCT TGCAAGACTA TAAGATTCAA TCTGCCCTGC TGGTGCCCAC ACTATTTAGC TTCTTCGCTA AGAGCACTCT CATCGACAAG TACGACCTAA GCAACTTGCA CGAGATCGCC AGCGGCGGGG CGCCGCTCAG CAAGGAGGTA GGTGAGGCCG TGGCCAAACG CTTCCACCTA CCAGGCATCC GCCAGGGCTA CGGCCTGACA GAAACAACCA GCGCCATTCT GATCACCCCC GAAGGGGACG ACAAGCCTGG CGCAGTAGGC AAGGTGGTGC CCTTCTTCGA GGCTAAGGTG GTGGACTTGG ACACCGGTAA GACACTGGGT GTGAACCAGC GCGGCGAGCT GTGCGTCCGT GGCCCCATGA TCATGAGCGG CTACGTTAAC AACCCCGAGG CTACAAACGC TCTCATCGAC AAGGACGGCT GGCTGCACAG CGGCGACATC GCCTACTGGG ACGAGGACGA GCACTTCTTC ATCGTGGACC GGCTGAAGAG CCTGATCAAA TACAAGGGCT ACCAGGTAGC CCCAGCCGAA CTGGAGAGCA TCCTGCTGCA ACACCCCAAC ATCTTCGACG CCGGGGTCGC CGGCCTGCCC GACGACGATG CCGGCGAGCT GCCCGCCGCA GTCGTCGTGC TGGAACACGG TAAAACCATG ACCGAGAAGG AGATCGTGGA CTATGTGGCC AGCCAGGTTA CAACCGCCAA GAAGCTGCGC GGTGGTGTTG TGTTCGTGGA CGAGGTGCCT AAAGGACTGA CCGGCAAGTT GGACGCCCGC AAGATCCGCG AGATTCTCAT TAAGGCCAAG AAGGGCGGCA AGATCGCCGT GTAATAATTC TAGAGTCGGG GCGGCCGGCC GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA AAATCGATAA GGATCCGTTT GCGTATTGGG CGCTCTTCCG CTGATCTGCG CAGCACCATG GCCTGAAATA ACCTCTGAAA GAGGAACTTG GTTAGCTACC TTCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCGATT CTTCTGACAC TAGCGCCACC ATGAAGAAGC CCGAACTCAC CGCTACCAGC GTTGAAAAAT TTCTCATCGA GAAGTTCGAC AGTGTGAGCG ACCTGATGCA GTTGTCGGAG GGCGAAGAGA GCCGAGCCTT CAGCTTCGAT GTCGGCGGAC GCGGCTATGT ACTGCGGGTG AATAGCTGCG CTGATGGCTT CTACAAAGAC CGCTACGTGT ACCGCCACTT CGCCAGCGCT GCACTACCCA TCCCCGAAGT GTTGGACATC GGCGAGTTCA GCGAGAGCCT GACATACTGC ATCAGTAGAC GCGCCCAAGG CGTTACTCTC CAAGACCTCC CCGAAACAGA GCTGCCTGCT GTGTTACAGC CTGTCGCCGA AGCTATGGAT GCTATTGCCG CCGCCGACCT CAGTCAAACC AGCGGCTTCG GCCCATTCGG GCCCCAAGGC ATCGGCCAGT ACACAACCTG GCGGGATTTC ATTTGCGCCA TTGCTGATCC CCATGTCTAC CACTGGCAGA CCGTGATGGA CGACACCGTG TCCGCCAGCG TAGCTCAAGC CCTGGACGAA CTGATGCTGT GGGCCGAAGA CTGTCCCGAG GTGCGCCACC TCGTCCATGC CGACTTCGGC AGCAACAACG TCCTGACCGA CAACGGCCGC ATCACCGCCG TAATCGACTG GTCCGAAGCT ATGTTCGGGG ACAGTCAGTA CGAGGTGGCC AACATCTTCT TCTGGCGGCC CTGGCTGGCT TGCATGGAGC AGCAGACTCG CTACTTCGAG CGCCGGCATC CCGAGCTGGC CGGCAGCCCT CGTCTGCGAG CCTACATGCT GCGCATCGGC CTGGATCAGC TCTACCAGAG CCTCGTGGAC GGCAACTTCG ACGATGCTGC CTGGGCTCAA GGCCGCTGCG ATGCCATCGT CCGCAGCGGG GCCGGCACCG TCGGTCGCAC ACAAATCGCT CGCCGGAGCG CAGCCGTATG GACCGACGGC TGCGTCGAGG TGCTGGCCGA CAGCGGCAAC CGCCGGCCCA GTACACGACC GCGCGCTAAG GAGGTAGGTC GAGTTTAAAC TCTAGAACCG GTCATGGCCG CAATAAAATA TCTTTATTTT CATTACATCT GTGTGTTGGT TTTTTGTGTG TTCGAACTAG ATGCTGTCGA CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC GGTGGGCGCG GGGCATGACT ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC TCGTAGGACA GGTGCCGGCA GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGCGGCC GCAAATGCTA AACCACTGCA GTGGTTACCA GTGCTTGATC AGTGAGGCAC CGATCTCAGC GATCTGCCTA TTTCGTTCGT CCATAGTGGC CTGACTCCCC GTCGTGTAGA TCACTACGAT TCGTGAGGGC TTACCATCAG GCCCCAGCGC AGCAATGATG CCGCGAGAGC CGCGTTCACC GGCCCCCGAT TTGTCAGCAA TGAACCAGCC AGCAGGGAGG GCCGAGCGAA GAAGTGGTCC TGCTACTTTG TCCGCCTCCA TCCAGTCTAT GAGCTGCTGT CGTGATGCTA GAGTAAGAAG TTCGCCAGTG AGTAGTTTCC GAAGAGTTGT GGCCATTGCT ACTGGCATCG TGGTATCACG CTCGTCGTTC GGTATGGCTT CGTTCAACTC TGGTTCCCAG CGGTCAAGCC GGGTCACATG ATCACCCATA TTATGAAGAA ATGCAGTCAG CTCCTTAGGG CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCGGTGTTGT CGCTCATGGT AATGGCAGCA CTACACAATT CTCTTACCGT CATGCCATCC GTAAGATGCT TTTCCGTGAC CGGCGAGTAC TCAACCAAGT CGTTTTGTGA GTAGTGTATA CGGCGACCAA GCTGCTCTTG CCCGGCGTCT ATACGGGACA ACACCGCGCC ACATAGCAGT ACTTTGAAAG TGCTCATCAT CGGGAATCGT TCTTCGGGGC GGAAAGACTC AAGGATCTTG CCGCTATTGA GATCCAGTTC GATATAGCCC ACTCTTGCAC CCAGTTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC GGGGTGTGCA AAAACAGGCA AGCAAAATGC CGCAAAGAAG GGAATGAGTG CGACACGAAA ATGTTGGATG CTCATACTCG TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTACTA GTACGTCTCT CAAGGATAAG TAAGTAATAT TAAGGTACGG GAGGTATTGG ACAGGCCGCA ATAAAATATC TTTATTTTCA TTACATCTGT GTGTTGGTTT TTTGTGTGAA TCGATAGTAC T
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