pCMVβ-p300-myc
$142.00
In stock
SKU
PL82406
EP300;KAT3B;RSTS2
100μg liquid plasmid(Instantly):$163
| name | pCMVβ-p300-myc | another name | apCMVβ-p300-myc |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | P0691-R：GGCCTGATTTGGGAGCATAT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCTGGC TAGCGATGAC CCTGCTGATT GGTTCGCTGA CCATTTCCGG GTGCGGGACG GCGTTACCAG AAACTCAGAA GGTTCGTCCA ACCAAACCGA CTCTGACGGC AGTTTACGAG AGAGATGATA GGGTCTGCTT CAGTAAGCCA GATGCTACAC AATTAGGCTT GTACATATTG TCGTTAGAAC GCGGCTACAA TTAATACATA ACCTTATGTA TCATACACAT ACGATTTAGG TGACACTATA GAATACACGG AATTCGAGCT CGCCCCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG GGGATCGCGG CCGCTCTAGA ACTAGTGGAT CCCCCGGGCC GAAGAAGAGA TTTCCTGAGG ATTCTGGTTT TCCTCGCTTG TATCTCCGAA AGAATTAAAA ATGGCCGAGA ATGTGGTGGA ACCGGGGCCG CCTTCAGCCA AGCGGCCTAA ACTCTCATCT CCGGCCCTCT CGGCGTCCGC CAGCGATGGC ACAGATTTTG GCTCTCTATT TGACTTGGAG CACGACTTAC CAGATGAATT AATCAACTCT ACAGAATTGG GACTAACCAA TGGTGGTGAT ATTAATCAGC TTCAGACAAG TCTTGGCATG GTACAAGATG CAGCTTCTAA ACATAAACAG CTGTCAGAAT TGCTGCGATC TGGTAGTTCC CCTAACCTCA ATATGGGAGT TGGTGGCCCA GGTCAAGTCA TGGCCAGCCA GGCCCAACAG AGCAGTCCTG GATTAGGTTT GATAAATAGC ATGGTCAAAA GCCCAATGAC ACAGGCAGGC TTGACTTCTC CCAACATGGG GATGGGCACT AGTGGACCAA ATCAGGGTCC TACGCAGTCA ACAGGTATGA TGAACAGTCC AGTAAATCAG CCTGCCATGG GAATGAACAC AGGGATGAAT GCGGGCATGA ATCCTGGAAT GTTGGCTGCA GGCAATGGAC AAGGGATAAT GCCTAATCAA GTCATGAACG GTTCAATTGG AGCAGGCCGA GGGCGACAGA ATATGCAGTA CCCAAACCCA GGCATGGGAA GTGCTGGCAA CTTACTGACT GAGCCTCTTC AGCAGGGCTC TCCCCAGATG GGAGGACAAA CAGGATTGAG AGGCCCCCAG CCTCTTAAGA TGGGAATGAT GAACAACCCC AATCCTTATG GTTCACCATA TACTCAGAAT CCTGGACAGC AGATTGGAGC CAGTGGCCTT GGTCTCCAGA TTCAGACAAA AACTGTACTA TCAAATAACT TATCTCCATT TGCTATGGAC AAAAAGGCAG TTCCTGGTGG AGGAATGCCC AACATGGGTC AACAGCCAGC CCCGCAGGTC CAGCAGCCAG GTCTGGTGAC TCCAGTTGCC CAAGGGATGG GTTCTGGAGC ACATACAGCT GATCCAGAGA AGCGCAAGCT CATCCAGCAG CAGCTTGTTC TCCTTTTGCA TGCTCACAAG TGCCAGCGCC GGGAACAGGC CAATGGGGAA GTGAGGCAGT GCAACCTTCC CCACTGTCGC ACAATGAAGA ATGTCCTAAA CCACATGACA CACTGCCAGT CAGGCAAGTC TTGCCAAGTG GCACACTGTG CATCTTCTCG ACAAATCATT TCACACTGGA AGAATTGTAC AAGACATGAT TGTCCTGTGT GTCTCCCCCT CAAAAATGCT GGTGATAAGA GAAATCAACA GCCAATTTTG ACTGGAGCAC CCGTTGGACT TGGAAATCCT AGCTCTCTAG GGGTGGGTCA ACAGTCTGCC CCCAACCTAA GCACTGTTAG TCAGATTGAT CCCAGCTCCA TAGAAAGAGC CTATGCAGCT CTTGGACTAC CCTATCAAGT AAATCAGATG CCGACACAAC CCCAGGTGCA AGCAAAGAAC CAGCAGAATC AGCAGCCTGG GCAGTCTCCC CAAGGCATGC GGCCCATGAG CAACATGAGT GCTAGTCCTA TGGGAGTAAA TGGAGGTGTA GGAGTTCAAA CGCCGAGTCT TCTTTCTGAC TCAATGTTGC ATTCAGCCAT AAATTCTCAA AACCCAATGA TGAGTGAAAA TGCCAGTGTG CCCTCCCTGG GTCCTATGCC AACAGCAGCT CAACCATCCA CTACTGGAAT TCGGAAACAG TGGCACGAAG ATATTACTCA GGATCTTCGA AATCATCTTG TTCACAAACT CGTCCAAGCC ATATTTCCTA CGCCGGATCC TGCTGCTTTA AAAGACAGAC GGATGGAAAA CCTAGTTGCA TATGCTCGGA AAGTTGAAGG GGACATGTAT GAATCTGCAA ACAATCGAGC GGAATACTAC CACCTTCTAG CTGAGAAAAT CTATAAGATC CAGAAAGAAC TAGAAGAAAA ACGAAGGACC AGACTACAGA AGCAGAACAT GCTACCAAAT GCTGCAGGCA TGGTTCCAGT TTCCATGAAT CCAGGGCCTA ACATGGGACA GCCGCAACCA GGAATGACTT CTAATGGCCC TCTACCTGAC CCAAGTATGA TCCGTGGCAG TGTGCCAAAC CAGATGATGC CTCGAATAAC TCCACAATCT GGTTTGAATC AATTTGGCCA GATGAGCATG GCCCAGCCCC CTATTGTACC CCGGCAAACC CCTCCTCTTC AGCACCATGG ACAGTTGGCT CAACCTGGAG CTCTCAACCC GCCTATGGGC TATGGGCCTC GTATGCAACA GCCTTCCAAC CAGGGCCAGT TCCTTCCTCA GACTCAGTTC CCATCACAGG GAATGAATGT AACAAATATC CCTTTGGCTC CGTCCAGCGG TCAAGCTCCA GTGTCTCAAG CACAAATGTC TAGTTCTTCC TGCCCGGTGA ACTCTCCTAT AATGCCTCCA GGGTCTCAGG GGAGCCACAT TCACTGTCCC CAGCTTCCTC AACCAGCTCT TCATCAGAAT TCACCCTCGC CTGTACCTAG TCGTACCCCC ACCCCTCACC ATACTCCCCC AAGCATAGGG GCTCAGCAGC CACCAGCAAC AACAATTCCA GCCCCTGTTC CTACACCTCC TGCCATGCCA CCTGGGCCAC AGTCCCAGGC TCTACATCCC CCTCCAAGGC AGACACCTAC ACCACCAACA ACACAACTTC CCCAACAAGT GCAGCCTTCA CTTCCTGCTG CACCTTCTGC TGACCAGCCC CAGCAGCAGC CTCGCTCACA GCAGAGCACA GCAGCGTCTG TTCCTACCCC AACAGCACCG CTGCTTCCTC CGCAGCCTGC AACTCCACTT TCCCAGCCAG CTGTAAGCAT TGAAGGACAG GTATCAAATC CTCCATCTAC TAGTAGCACA GAAGTGAATT CTCAGGCCAT TGCTGAGAAG CAGCCTTCCC AGGAAGTGAA GATGGAGGCC AAAATGGAAG TGGATCAACC AGAACCAGCA GATACTCAGC CGGAGGATAT TTCAGAGTCT AAAGTGGAAG ACTGTAAAAT GGAATCTACC GAAACAGAAG AGAGAAGCAC TGAGTTAAAA ACTGAAATAA AAGAGGAGGA AGACCAGCCA AGTACTTCAG CTACCCAGTC ATCTCCGGCT CCAGGACAGT CAAAGAAAAA GATTTTCAAA CCAGAAGAAC TACGACAGGC ACTGATGCCA ACTTTGGAGG CACTTTACCG TCAGGATCCA GAATCCCTTC CCTTTCGTCA ACCTGTGGAC CCTCAGCTTT TAGGAATCCC TGATTACTTT GATATTGTGA AGAGCCCCAT GGATCTTTCT ACCATTAAGA GGAAGTTAGA CACTGGACAG TATCAGGAGC CCTGGCAGTA TGTCGATGAT ATTTGGCTTA TGTTCAATAA TGCCTGGTTA TATAACCGGA AAACATCACG GGTATACAAA TACTGCTCCA AGCTCTCTGA GGTCTTTGAA CAAGAAATTG ACCCAGTGAT GCAAAGCCTT GGATACTGTT GTGGCAGAAA GTTGGAGTTC TCTCCACAGA CACTGTGTTG CTACGGCAAA CAGTTGTGCA CAATACCTCG TGATGCCACT TATTACAGTT ACCAGAACAG GTATCATTTC TGTGAGAAGT GTTTCAATGA GATCCAAGGG GAGAGCGTTT CTTTGGGGGA TGACCCTTCC CAGCCTCAAA CTACAATAAA TAAAGAACAA TTTTCCAAGA GAAAAAATGA CACACTGGAT CCTGAACTGT TTGTTGAATG TACAGAGTGC GGAAGAAAGA TGCATCAGAT CTGTGTCCTT CACCATGAGA TCATCTGGCC TGCTGGATTC GTCTGTGATG GCTGTTTAAA GAAAAGTGCA CGAACTAGGA AAGAAAATAA GTTTTCTGCT AAAAGGTTGC CATCTACCAG ACTTGGCACC TTTCTAGAGA ATCGTGTGAA TGACTTTCTG AGGCGACAGA ATCACCCTGA GTCAGGAGAG GTCACTGTTA GAGTAGTTCA TGCTTCTGAC AAAACCGTGG AAGTAAAACC AGGCATGAAA GCAAGGTTTG TGGACAGTGG AGAGATGGCA GAATCCTTTC CATACCGAAC CAAAGCCCTC TTTGCCTTTG AAGAAATTGA TGGTGTTGAC CTGTGCTTCT TTGGCATGCA TGTTCAAGAG TATGGCTCTG ACTGCCCTCC ACCCAACCAG AGGAGAGTAT ACATATCTTA CCTCGATAGT GTTCATTTCT TCCGTCCTAA ATGCTTGAGG ACTGCAGTCT ATCATGAAAT CCTAATTGGA TATTTAGAAT ATGTCAAGAA ATTAGGTTAC ACAACAGGGC ATATTTGGGC ATGTCCACCA AGTGAGGGAG ATGATTATAT CTTCCATTGC CATCCTCCTG ACCAGAAGAT ACCCAAGCCC AAGCGACTGC AGGAATGGTA CAAAAAAATG CTTGACAAGG CTGTATCAGA GCGTATTGTC CATGACTACA AGGATATTTT TAAACAAGCT ACTGAAGATA GATTAACAAG TGCAAAGGAA TTGCCTTATT TCGAGGGTGA TTTCTGGCCC AATGTTCTGG AAGAAAGCAT TAAGGAACTG GAACAGGAGG AAGAAGAGAG AAAACGAGAG GAAAACACCA GCAATGAAAG CACAGATGTG ACCAAGGGAG ACAGCAAAAA TGCTAAAAAG AAGAATAATA AGAAAACCAG CAAAAATAAG AGCAGCCTGA GTAGGGGCAA CAAGAAGAAA CCCGGGATGC CCAATGTATC TAACGACCTC TCACAGAAAC TATATGCCAC CATGGAGAAG CATAAAGAGG TCTTCTTTGT GATCCGCCTC ATTGCTGGCC CTGCTGCCAA CTCCCTGCCT CCCATTGTTG ATCCTGATCC TCTCATCCCC TGCGATCTGA TGGATGGTCG GGATGCGTTT CTCACGCTGG CAAGGGACAA GCACCTGGAG TTCTCTTCAC TCCGAAGAGC CCAGTGGTCC ACCATGTGCA TGCTGGTGGA GCTGCACACG CAGAGCCAGG ACCGCTTTGT CTACACCTGC AATGAATGCA AGCACCATGT GGAGACACGC TGGCACTGTA CTGTCTGTGA GGATTATGAC TTGTGTATCA CCTGCTATAA CACTAAAAAC CATGACCACA AAATGGAGAA ACTAGGCCTT GGCTTAGATG ATGAGAGCAA CAACCAGCAG GCTGCAGCCA CCCAGAGCCC AGGCGATTCT CGCCGCCTGA GTATCCAGCG CTGCATCCAG TCTCTGGTCC ATGCTTGCCA GTGTCGGAAT GCCAATTGCT CACTGCCATC CTGCCAGAAG ATGAAGCGGG TTGTGCAGCA TACCAAGGGT TGCAAACGGA AAACCAATGG CGGGTGCCCC ATCTGCAAGC AGCTCATTGC CCTCTGCTGC TACCATGCCA AGCACTGCCA GGAGAACAAA TGCCCGGTGC CGTTCTGCCT AAACATCAAG CAGAAGCTCC GGCAGCAACA GCTGCAGCAC CGACTACAGC AGGCCCAAAT GCTTCGCAGG AGGATGGCCA GCATGCAGCG GACTGGTGTG GTTGGGCAGC AACAGGGCCT CCCTTCCCCC ACTCCTGCCA CTCCAACGAC ACCAACTGGC CAACAGCCAA CCACCCCGCA GACGCCCCAG CCCACTTCTC AGCCTCAGCC TACCCCTCCC AATAGCATGC CACCCTACTT GCCCAGGACT CAAGCTGCTG GCCCTGTGTC CCAGGGTAAG GCAGCAGGCC AGGTGACCCC TCCAACCCCT CCTCAGACTG CTCAGCCACC CCTTCCAGGG CCCCCACCTG CAGCAGTGGA AATGGCAATG CAGATTCAGA GAGCAGCGGA GACGCAGCGC CAGATGGCCC ACGTGCAAAT TTTTCAAAGG CCAATCCAAC ACCAGATGCC CCCGATGACT CCCATGGCCC CCATGGGTAT GAACCCACCT CCCATGACCA GAGGTCCCAG TGGGCATTTG GAGCCAGGGA TGGGACCGAC AGGGATGCAG CAACAGCCAC CCTGGAGCCA AGGAGGATTG CCTCAGCCCC AGCAACTACA GTCTGGGATG CCAAGGCCAG CCATGATGTC AGTGGCCCAG CATGGTCAAC CTTTGAACAT GGCTCCACAA CCAGGATTGG GCCAGGTAGG TATCAGCCCA CTCAAACCAG GCACTGTGTC TCAACAAGCC TTACAAAACC TTTTGCGGAC TCTCAGGTCT CCCAGCTCTC CCCTGCAGCA GCAACAGGTG CTTAGTATCC TTCACGCCAA CCCCCAGCTG TTGGCTGCAT TCATCAAGCA GCGGGCTGCC AAGTATGCCA ACTCTAATCC ACAACCCATC CCTGGGCAGC CTGGCATGCC CCAGGGGCAG CCAGGGCTAC AGCCACCTAC CATGCCAGGT CAGCAGGGGG TCCACTCCAA TCCAGCCATG CAGAACATGA ATCCAATGCA GGCGGGCGTT CAGAGGGCTG GCCTGCCCCA GCAGCAACCA CAGCAGCAAC TCCAGCCACC CATGGGAGGG ATGAGCCCCC AGGCTCAGCA GATGAACATG AACCACAACA CCATGCCTTC ACAATTCCGA GACATCTTGA GACGACAGCA AATGATGCAA CAGCAGCAGC AACAGGGAGC AGGGCCAGGA ATAGGCCCTG GAATGGCCAA CCATAACCAG TTCCAGCAAC CCCAAGGAGT TGGCTACCCA CCACAGCAGC AGCAGCGGAT GCAGCATCAC ATGCAACAGA TGCAACAAGG AAATATGGGA CAGATAGGCC AGCTTCCCCA GGCCTTGGGA GCAGAGGCAG GTGCCAGTCT ACAGGCCTAT CAGCAGCGAC TCCTTCAGCA ACAGATGGGG TCCCCTGTTC AGCCCAACCC CATGAGCCCC CAGCAGCATA TGCTCCCAAA TCAGGCCCAG TCCCCACACC TACAAGGCCA GCAGATCCCT AATTCTCTCT CCAATCAAGT GCGCTCTCCC CAGCCTGTCC CTTCTCCACG GCCACAGTCC CAGCCCCCCC ACTCCAGTCC TTCCCCAAGG ATGCAGCCTC AGCCTTCTCC ACACCACGTT TCCCCACAGA CAAGTTCCCC ACATCCTGGA CTGGTAGCTG CCCAGGCCAA CCCCATGGAA CAAGGGCATT TTGCCAGCCC GGACCAGAAT TCAATGCTTT CTCAGCTTGC TAGCAATCCA GGCATGGCAA ACCTCCATGG TGCAAGCGCC ACGGACCTGG GACTCAGCAC CGATAACTCA GACTTGAATT CAAACCTCTC ACAGAGTACA CTAGACATAC ACGAACAAAA ACTTATTTCT GAAGAAGACC TATAGAAGCT TATGCATAGA TCTATGCATA GATCTATGCA TAGATCCTGA GAACTTCAGG GTGAGTTTGG GGACCCTTGA TTGTTCTTTC TTTTTCGCTA TTGTAAAATT CATGTTATAT GGAGGGGGCA AAGTTTTCAG GGTGTTGTTT AGAATGGGAA GATGTCCCTT GTATCACCAT GGACCCTCAT GATAATTTTG TTTCTTTCAC TTTCTACTCT GTTGACAACC ATTGTCTCCT CTTATTTTCT TTTCATTTTC TGTAACTTTT TCGTTAAACT TTAGCTTGCA TTTGTAACGA ATTTTTAAAT TCACTTTTGT TTATTTGTCA GATTGTAAGT ACTTTCTCTA ATCACTTTTT TTTCAAGGCA ATCAGGGTAT ATTATATTGT ACTTCAGCAC AGTTTTAGAG AACAATTGTT ATAATTAAAT GATAAGGTAG AATATTTCTG CATATAAATT CTGGCTGGCG TGGAAATATT CTTATTGGTA GAAACAACTA CACCCTGGTC ATCATCCTGC CTTTCTCTTT ATGGTTACAA TGATATACAC TGTTTGAGAT GAGGATAAAA TACTCTGAGT CCAAACCGGG CCCCTCTGCT AACCATGTTC ATGCCTTCTT CTCTTTCCTA CAGCTCCTGG GCAACGTGCT GGTTGTTGTG CTGTCTCATC ATTTTGGCAA AGAATTCACT CCTCAGGTGC AGGCTGCCTA TCAGAAGGTG GTGGCTGGTG TGGCCAATGC CCTGGCTCAC AAATACCACT GAGATCTTTT TCCCTCTGCC AAAAATTATG GGGACATCAT GAAGCCCCTT GAGCATCTGA CTTCTGGCTA ATAAAGGAAA TTTATTTTCA TTGCAATAGT GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT GGGAGGGCAA ATCATTTAAA ACATCAGAAT GAGTATTTGG TTTAGAGTTT GGCAACATAT GCCCATATGC TGGCTGCCAT GAACAAAGGT TGGCTATAAA GAGGTCATCA GTATATGAAA CAGCCCCCTG CTGTCCATTC CTTATTCCAT AGAAAAGCCT TGACTTGAGG TTAGATTTTT TTTATATTTT GTTTTGTGTT ATTTTTTTCT TTAACATCCC TAAAATTTTC CTTACATGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGT
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