pENTR5-UbCp
$99.00
In stock
SKU
PL82374
pENTR5'-UbCp
100μg liquid plasmid(Instantly):$120
| name | pENTR5-UbCp | another name | apENTR5-UbCp |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF,GateWay |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | UBC | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F： | |
| 3′ sequencing primer | M13R： | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAACGGAAGA GCGCCCAATA CGCAAACCGC CCTCTCCCCG CGCGTTGGCC GATTCATTAA ATGCAGCTGG CACGACAGGT TTTCCCGACT GGAAAGCGGG CAGTGGAGCG CAACGCAATT AATACCCGTA CCCGCTTACC CAGGAAAAGT TTGTAAAAAC GCAAAAAAGG CCATCCCTCC AGGATGGCCT TCTGCTTAAT TTGGATGCCT GGCAAGTTTA TGGGGGGGGT CTGCCCGCCA CCCTCCGGGC CGTTGCTTCC ACAACGTTCA AATCCGCTCC CCGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA CAACAGATAA AACGAAAGGC CCAGTTCCCG ACTGAGCCTT TCGTTTTATT TGATGCCTGG CAGTTCCCTA CTCTCGCGTT AACGCTAGCA TGGATGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTCTTAAGCT CGGGCCCGCG TTAACGCTAC CATGGAGCTC CAAATAATGA TTTTATTTTG ACTGATAGTG ACCTGTTCGT TGCAACAAAT TGATAAGCAA TGCTTTTTTA TAATGCCAAC TTTGTATAGA AAAGTTGGGC CTCGCGCCGG GTTTTGGCGC CTCCCGCGGG CGCCCCCCTC CTCACGGCGA GCGCTGCCAC GTCAGACGAA GGGCGCAGGA GCGTCCTGAT CCTTCCGCCC GGACGCTCAG GACAGCGGCC CGCTGCTCAT AAGACTCGGC CTTAGAACCC CAGTATCAGC AGAAGGACAT TTTAGGACGG GACTTGGGTG ACTCTAGGGC ACTGGTTTTC TTTCCAGAGA GCGGAACAGG CGAGGAAAAG TAGTCCCTTC TCGGCGATTC TGCGGAGGGA TCTCCGTGGG GCGGTGAACG CCGATGATTA TATAAGGACG CGCCGGGTGT GGCACAGCTA GTTCCGTCGC AGCCGGGATT TGGGTCGCGG TTCTTGTTTG TGGATCGCTG TGATCGTCAC TTGGTGAGTA GCGGGCTGCT GGGCTGGCCG GGGCTTTCGT GGCCGCCGGG CCGCTCGGTG GGACGGAAGC GTGTGGAGAG ACCGCCAAGG GCTGTAGTCT GGGTCCGCGA GCAAGGTTGC CCTGAACTGG GGGTTGGGGG GAGCGCAGCA AAATGGCGGC TGTTCCCGAG TCTTGAATGG AAGACGCTTG TGAGGCGGGC TGTGAGGTCG TTGAAACAAG GTGGGGGGCA TGGTGGGCGG CAAGAACCCA AGGTCTTGAG GCCTTCGCTA ATGCGGGAAA GCTCTTATTC GGGTGAGATG GGCTGGGGCA CCATCTGGGG ACCCTGACGT GAAGTTTGTC ACTGACTGGA GAACTCGGTT TGTCGTCTGT TGCGGGGGCG GCAGTTATGC GGTGCCGTTG GGCAGTGCAC CCGTACCTTT GGGAGCGCGC GCCCTCGTCG TGTCGTGACG TCACCCGTTC TGTTGGCTTA TAATGCAGGG TGGGGCCACC TGCCGGTAGG TGTGCGGTAG GCTTTTCTCC GTCGCAGGAC GCAGGGTTCG GGCCTAGGGT AGGCTCTCCT GAATCGACAG GCGCCGGACC TCTGGTGAGG GGAGGGATAA GTGAGGCGTC AGTTTCTTTG GTCGGTTTTA TGTACCTATC TTCTTAAGTA GCTGAAGCTC CGGTTTTGAA CTATGCGCTC GGGGTTGGCG AGTGTGTTTT GTGAAGTTTT TTAGGCACCT TTTGAAATGT AATCATTTGG GTCAATATGT AATTTTCAGT GTTAGACTAG TAAATTGTCC GCTAAATTCT GGCCGTTTTT GGCTTTTTTG TTAGACCAAG TTTGTACAAA AAAGTTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATGC AGTCACTATG AATCAACTAC TTAGATGGTA TTAGTGACCT GTAGAATTCG AGCTCTAGAG CTGCAGGGCG GCCGCGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAAATATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGAAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTACTCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T
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