pHT43-His
$128.00
In stock
SKU
PL82366
pHT43-6×His
100μg liquid plasmid(Instantly):$149
| name | pHT43-His | another name | apHT43-His |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression,Secretion expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Chl | Fluorescent labeling | |
| Promoter | Pgrac | Replicon | pUC,repB |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | pHT-R：agcaagtcatctccaaattctt | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTAAGTTATT GGTATGACTG GTTTTAAGCG CAAAAAAAGT TGCTTTTTCG TACCTATTAA TGTATCGTTT TAGAAAACCG ACTGTAAAAA GTACAGTCGG CATTATCTCA TATTATAAAA GCCAGTCATT AGGCCTATCT GACAATTCCT GAATAGAGTT CATAAACAAT CCTGCATGAT AACCATCACA AACAGAATGA TGTACCTGTA AAGATAGCGG TAAATATATT GAATTACCTT TATTAATGAA TTTTCCTGCT GTAATAATGG GTAGAAGGTA ATTACTATTA TTATTGATAT TTAAGTTAAA CCCAGTAAAT GAAGTCCATG GAATAATAGA AAGAGAAAAA GCATTTTCAG GTATAGGTGT TTTGGGAAAC AATTTCCCCG AACCATTATA TTTCTCTACA TCAGAAAGGT ATAAATCATA AAACTCTTTG AAGTCATTCT TTACAGGAGT CCAAATACCA GAGAATGTTT TAGATACACC ATCAAAAATT GTATAAAGTG GCTCTAACTT ATCCCAATAA CCTAACTCTC CGTCGCTATT GTAACCAGTT CTAAAAGCTG TATTTGAGTT TATCACCCTT GTCACTAAGA AAATAAATGC AGGGTAAAAT TTATATCCTT CTTGTTTTAT GTTTCGGTAT AAAACACTAA TATCAATTTC TGTGGTTATA CTAAAAGTCG TTTGTTGGTT CAAATAATGA TTAAATATCT CTTTTCTCTT CCAATTGTCT AAATCAATTT TATTAAAGTT CATTTGATAT GCCTCCTAAA TTTTTATCTA AAGTGAATTT AGGAGGCTTA CTTGTCTGCT TTCTTCATTA GAATCAATCC TTTTTTAAAA GTCAATATTA CTGTAACATA AATATATATT TTAAAAATAT CCCACTTTAT CCAATTTTCG TTTGTTGAAC TAATGGGTGC TTTAGTTGAA GAATAAAGAC CACATTAAAA AATGTGGTCT TTTGTGTTTT TTTAAAGGAT TTGAGCGTAG CGAAAAATCC TTTTCTTTCT TATCTTGATA ATAAGGGTAA CTATTGCCGA TCGTCCATTC CGACAGCATC GCCAGTCACT ATGGCGTGCT GCTAGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCAGGC CTTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCC AGGGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT TTGATGGTGG TTGACGGCGG GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT ACCGAGATAT CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA ATTTGATTGC GAGTGAGATA TTTATGCCAG CCAGCCAGAC GCAGACGCGC CGAGACAGAA CTTAATGGGC CCGCTAACAG CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG CCCAGTCGCG TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGCACC GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC GCTGGCACCC AGTTGATCGG CGCGAGATTT AATCGCCGCG ACAATTTGCG ACGGCGCGTG CAGGGCCAGA CTGGAGGTGG CAACGCCAAT CAGCAACGAC TGTTTGCCCG CCAGTTGTTG TGCCACGCGG TTGGGAATGT AATTCAGCTC CGCCATCGCC GCTTCCACTT TTTCCCGCGT TTTCGCAGAA ACGTGGCTGG CCTGGTTCAC CACGCGGGAA ACGGTCTGAT AAGAGACACC GGCATACTCT GCGACATCGT ATAACGTTAC TGGTTTCATC AAAATCGTCT CCCTCCGTTT GAATATTTGA TTGATCGTAA CCAGATGAAG CACTCTTTCC ACTATCCCTA CAGTGTTATG GCTTGAACAA TCACGAAACA ATAATTGGTA CGTACGATCT TTCAGCCGAC TCAAACATCA AATCTTACAA ATGTAGTCTT TGAAAGTATT ACATATGTAA GATTTAAATG CAACCGTTTT TTCGGAAGGA AATGATGACC TCGTTTCCAC CGGAATTAGC TTGGTACCAG CTATTGTAAC ATAATCGGTA CGGGGGTGAA AAAGCTAACG GAAAAGGGAG CGGAAAAGAA TGATGTAAGC GTGAAAAATT TTTTATCTTA TCACTTGAAA TTGGAAGGGA GATTCTTTAT TATAAGAATT GTGGAATTGT GAGCGGATAA CAATTCCCAA TTAAAGGAGG AAGGATCAAT GATTCAAAAA CGAAAGCGGA CAGTTTCGTT CAGACTTGTG CTTATGTGCA CGCTGTTATT TGTCAGTTTG CCGATTACAA AAACATCAGC CGTAGGATCC ATGCGGCCGC TAACTAGTGT CACCGGTATC TAGAGTCGAC GTCCCGGGCA TCACCATCAT CATCACCAGC CCGCCTAATG AGCGGGCTTT TTTCACGTCA CGCGTCCATG GAGATCTTTG TCTGCAACTG AAAAGTTTAT ACCTTACCTG GAACAAATGG TTGAAACATA CGAGGCTAAT ATCGGCTTAT TAGGAATAGT CCCTGTACTA ATAAAATCAG GTGGATCAGT TGATCAGTAT ATTTTGGACG AAGCTCGGAA AGAATTTGGA GATGACTTGC TTAATTCCAC AATTAAATTA AGGGAAAGAA TAAAGCGATT TGATGTTCAA GGAATCACGG AAGAAGATAC TCATGATAAA GAAGCTCTAA AACTATTCAA TAACCTTACA ATGGAATTGA TCGAAAGGGT GGAAGGTTAA TGGTACGAAA ATTAGGGGAT CTACCTAGAA AGCCACAAGG CGATAGGTCA AGCTTAAAGA ACCCTTACAT GGATCTTACA GATTCTGAAA GTAAAGAAAC AACAGAGGTT AAACAAACAG AACCAAAAAG AAAAAAAGCA TTGTTGAAAA CAATGAAAGT TGATGTTTCA ATCCATAATA AGATTAAATC GCTGCACGAA ATTCTGGCAG CATCCGAAGG GAATTCATAT TACTTAGAGG ATACTATTGA GAGAGCTATT GATAAGATGG TTGAGACATT ACCTGAGAGC CAAAAAACTT TTTATGAATA TGAATTAAAA AAAAGAACCA ACAAAGGCTG AGACAGACTC CAAACGAGTC TGTTTTTTTA AAAAAAATAT TAGGAGCATT GAATATATAT TAGAGAATTA AGAAAGACAT GGGAATAAAA ATATTTTAAA TCCAGTAAAA ATATGATAAG ATTATTTCAG AATATGAAGA ACTCTGTTTG TTTTTGATGA AAAAACAAAC AAAAAAAATC CACCTAACGG AATCTCAATT TAACTAACAG CGGCCAAACT GAGAAGTTAA ATTTGAGAAG GGGAAAAGGC GGATTTATAC TTGTATTTAA CTATCTCCAT TTTAACATTT TATTAAACCC CATACAAGTG AAAATCCTCT TTTACACTGT TCCTTTAGGT GATCGCGGAG GGACATTATG AGTGAAGTAA ACCTAAAAGG AAATACAGAT GAATTAGTGT ATTATCGACA GCAAACCACT GGAAATAAAA TCGCCAGGAA GAGAATCAAA AAAGGGAAAG AAGAAGTTTA TTATGTTGCT GAAACGGAAG AGAAGATATG GACAGAAGAG CAAATAAAAA ACTTTTCTTT AGACAAATTT GGTACGCATA TACCTTACAT AGAAGGTCAT TATACAATCT TAAATAATTA CTTCTTTGAT TTTTGGGGCT ATTTTTTAGG TGCTGAAGGA ATTGCGCTCT ATGCTCACCT AACTCGTTAT GCATACGGCA GCAAAGACTT TTGCTTTCCT AGTCTACAAA CAATCGCTAA AAAAATGGAC AAGACTCCTG TTACAGTTAG AGGCTACTTG AAACTGCTTG AAAGGTACGG TTTTATTTGG AAGGTAAACG TCCGTAATAA AACCAAGGAT AACACAGAGG AATCCCCGAT TTTTAAGATT AGACGTAAGG TTCCTTTGCT TTCAGAAGAA CTTTTAAATG GAAACCCTAA TATTGAAATT CCAGATGACG AGGAAGCACA TGTAAAGAAG GCTTTAAAAA AGGAAAAAGA GGGTCTTCCA AAGGTTTTGA AAAAAGAGCA CGATGAATTT GTTAAAAAAA TGATGGATGA GTCAGAAACA ATTAATATTC CAGAGGCCTT ACAATATGAC ACAATGTATG AAGATATACT CAGTAAAGGA GAAATTCGAA AAGAAATCAA AAAACAAATA CCTAATCCTA CAACATCTTT TGAGAGTATA TCAATGACAA CTGAAGAGGA AAAAGTCGAC AGTACTTTAA AAAGCGAAAT GCAAAATCGT GTCTCTAAGC CTTCTTTTGA TACCTGGTTT AAAAACACTA AGATCAAAAT TGAAAATAAA AATTGTTTAT TACTTGTACC GAGTGAATTT GCATTTGAAT GGATTAAGAA AAGATATTTA GAAACAATTA AAACAGTCCT TGAAGAAGCT GGATATGTTT TCGAAAAAAT CGAACTAAGA AAAGTGCAAT AAACTGCTGA AGTATTTCAG CAGTTTTTTT TATTTAGAAA TAGTGAAAAA AATATAATCA GGGAGGTATC AATATTTAAT GAGTACTGAT TTAAATTTAT TTAGACTGGA ATTAATAATT AACACGTAGA CTAATTAAAA TTTAATGAGG GATAAAGAGG ATACAAAAAT ATTAATTTCA ATCCCTATTA AATTTTAACA AGGGGGGGAT TAAAATTTAA TTAGAGGTTT ATCCACAAGA AAAGACCCTA ATAAAATTTT TACTAGGGTT ATAACACTGA TTAATTTCTT AATGGGGGAG GGATTAAAAT TTAATGACAA AGAAAACAAT CTTTTAAGAA AAGCTTTTAA AAGATAATAA TAAAAAGAGC TTTGCGATTA AGCAAAACTC TTTACTTTTT CATTGACATT ATCAAATTCA TCGATTTCAA ATTGTTGTTG TATCATAAAG TTAATTCTGT TTTGCACAAC CTTTTCAGGA ATATAAAACA CATCTGAGGC TTGTTTTATA AACTCAGGGT CGCTAAAGTC AATGTAACGT AGCATATGAT ATGGTATAGC TTCCACCCAA GTTAGCCTTT CTGCTTCTTC TGAATGTTTT TCATATACTT CCATGGGTAT CTCTAAATGA TTTTCCTCAT GTAGCAAGGT ATGAGCAAAA AGTTTATGGA ATTGATAGTT CCTCTCTTTT TCTTCAACTT TTTTATCTAA AACAAACACT TTAACATCTG AGTCAATGTA AGCATAAGAT GTTTTTCCAG TCATAATTTC AATCCCAAAT CTTTTAGACA GAAATTCTGG ACGTAAATCT TTTGGTGAAA GAATTTTTTT ATGTAGCAAT ATATCCGATA CAGCACCTTC TAAAAGCGTT GGTGAATAGG GCATTTTACC TATCTCCTCT CATTTTGTGG AATAAAAATA GTCATATTCG TCCATCTACC TATCCTATTA TCGAACAGTT GAACTTTTTA ATCAAGGATC AGTCCTTTTT TTCATTATTC TTAAACTGTG CTCTTAACTT TAACAACTCG ATTTGTTTTT CCAGATCTCG AGGGTAACTA GCCTCGCCGA TCCCGCAAGA GGCCCGGCAG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCATG C
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