pLVTHM
$70.00
In stock
SKU
PL82335
100μg liquid plasmid(Instantly):$91
| name | pLVTHM | another name | apLVTHM |
| Insert Size (bp) | Plasmid host | Mammary cell,Lentiviral | |
| Plasmid uses | Interfere with silence,Tetracycline induction | Fragment type | shRNA |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | H1/Tet | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | pCMV-R：TCCAAACTCATCAATGTATC | |
| 3′ sequencing primer | WPRE-R：CATAGCGTAAAAGGAGCAACA | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTACTAAGTT TGTAGTACAT ATTTAACAAA TACAATTTCT TTAAAATGAA AATAATTCAG AGGAATCACA GGTTTAGAGT AAATGAAACC ACTAGGTAAT TGGCAGTGGT AATAGGGTAT GGGGTGGGAA GTTTGGGATG ATTTTGGTTA GCTTGAGTTA TCCAGTTGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCTC TAGTCAAGGC ACTATACATC AAATATTCCT TATTAACCCC TTTACAAATT AAAAAGCTAA AGGTACACAA TTTTTGAGCA TAGTTATTAA TAGCAGACAC TCTATGCCTG TGTGGAGTAA GAAAAAACAG TATGTTATGA TTATAACTGT TATGCCTACT TATAAAGGTT ACAGAATATT TTTCCATAAT TTTCTTGTAT AGCAGTGCAG CTTTTTCCTT TGTGGTGTAA ATAGCAAAGC AAGCAAGAGT TCTATTACTA AACACAGCAT GACTCAAAAA ACTTAGCAAT TCTGAAGGAA AGTCCTTGGG GTCTTCTACC TTTCTCTTCT TTTTTGGAGG AGTAGAATGT TGAGAGTCAG CAGTAGCCTC ATCATCACTA GATGGCATTT CTTCTGAGCA AAACAGGTTT TCCTCATTAA AGGCATTCCA CCACTGCTCC CATTCATCAG TTCCATAGGT TGGAATCTAA AATACACAAA CAATTAGAAT CAGTAGTTTA ACACATTATA CACTTAAAAA TTTTATATTT ACCTTAGAGC TTTAAATCTC TGTAGGTAGT TTGTCCAATT ATGTCACACC ACAGAAGTAA GGTTCCTTCA CAAAGATCCG GGGCCCACTC ATAAATCCAG TTGCCGCCAC GGTAGCCAAT CACCGTATCG TATAAATCAT CGTCGGTACG TTCGGCATCG CTCATCACAA TACGTGCCTG GACGTCGAGG ATTTCGCGTG GGTCAATGCC GCGCCAGATC CACATCAGAC GGTTAATCAT GCGATACCAG TGAGGGATGG TTTTACCATC AAGGGCCGAC TGCACAGGCG GTTGTGCGCC GTGATTAAAG CGGCGGACTA GCGTCGAGGT TTCAGGATGT TTAAAGCGGG GTTTGAACAG GGTTTCGCTC AGGTTTGCCT GTGTCATGGA TGCAGCCTCC AGAATACTTA CTGGAAACTA TTGTAACCCG CCTGAAGTTA AAAAGAACAA CGCCCGGCAG TGCCAGGCGT TGAAAAGATT AGCGACCGGA GATTGGCGGG ACGAATACGA CGCCCATATC CCACGGCTGT TCAATCCAGG TATCTTGCGG GATATCAACA ACATAGTCAT CAACCAGCGG ACGACCAGCC GGTTTTGCGA AGATGGTGAC AAAGTGCGCT TTTGGATACA TTTCACGAAT CGCAACCGCA GTACCACCGG TATCCACCAG GTCATCAATA ACGATGAAGC CTTCGCCATC GCCTTCTGCG CGTTTCAGCA CTTTAAGCTC GCGCTGGTTG TCGTGATCGT AGCTGGAAAT ACAAACGGTA TCGACATGAC GAATACCCAG TTCACGCGCC AGTAACGCAC CCGGTACAGA CCGCCACGGC TTACGGCAAT AATGCCTTTC CATTGTTCAG AAGGCATCAG TCGGCTTGCG AGTTTACGTG CATGGATCTG CAACATGTCC CAGGTGACGA TGTATTTTTC GCTCATGTGA AGTGTCCCAG CCTGTTTATC TACGGCTTAA AAAGTGTTCG AGGGGAAAAT AGGTTGCGCG AGATTATAGA GATCTGGCGC ACTAAAAACC AGTATTTCAC ATGAGTCCGC GTCTTTTTAC GCACTGCCTC TCCCTGACGC GGGATAAAGT GGTATTCTCA AACATATCTC GCAAGCCTGT CTTGTGTCCA AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCTGGCT AGCGATGACC CTGCTGATTG GTTCGCTGAC CATTTCCGGG TGCGGGACGG CGTTACCAGA AACTCAGAAG GTTCGTCCAA CCAAACCGAC TCTGACGGCA GTTTACGAGA GAGATGATAG GGTCTGCTTC AGTAAGCCAG ATGCTACACA ATTAGGCTTG TACATATTGT CGTTAGAACG CGGCTACAAT TAATACATAA CCTTATGTAT CATACACATA CGATTTAGGT GACACTATAG AATACACGGA ATTAATTCTA GATGCTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAAGCAAAAA GCAGCATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT CCCATGCGAC GGTATCGATA AGCTTGGAGA CGCGTGGTCT CATACAGAAC TTATAAGATT CCCAAATCCA AAGACATTTC ACGTTTATGG TGATTTCCCA GAACACATAG CGACATGCAA ATATTGCAGG GCGCCACTCC CCTGTCCCTC ACAGCCATCT TCCTGCCAGG GCGCACGCGC GCTGGGTGTT CCCGCCTAGT GACACTGGGC CCGCGATTCC TTGGAGCGGG TTGATGACGT CAGCGTTCGA ATTCCTGCAG CCCCGACTTT CACTTTTCTC TATCACTGAT AGGGAGTGGT AAACTCGACT TTCACTTTTC TCTATCACTG ATAGGGAGTG GTAAACTCGA CTTTCACTTT TCTCTATCAC TGATAGGGAG TGGTAAACTC GACTTTCACT TTTCTCTATC ACTGATAGGG AGTGGTAAAC TCGACTTTCA CTTTTCTCTA TCACTGATAG GGAGTGGTAA ACTCGACTTT CACTTTTCTC TATCACTGAT AGGGAGTGGT AAACTCGACT TTCACTTTTC TCTATCACTG ATAGGGAGTG GTAAACTCGA GGGGGATCCA CTAGCATGGT GAATTGATCC CATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGG CCATACGTAC CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC AGCAGCGGGC AAGGCAGGCG GCGATGAGTT CCGCCGTGGC AATAGGGAGG GGGAAAGCGA AAGTCCCGGA AAGGAGCTGA CAGGTGGTGG CAATGCCCCA ACCAGTGGGG GTTGCGTCAG CAAACACAGT GCACACCACG CCACGTTGCC TGACAACGGG CCACAACTCC TCATAAAGAG ACAGCAACCA GGATTTATAC AAGGAGGAGA AAATGAAAGC CATACGGGAA GCAATAGCAT GATACAAAGG CATTAAAGCA GCGTATCCAC ATAGCGTAAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA ATTTTGTAAT CCAGAGGTTG ATTATCATAT GACTAGTCCC CGAAGCTTGA GCTCGACTAG CTAGCTAGCT ACTAGCTAGT CGAGATCTGA GTCCGGACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG TTCACCTTGA TGCCGTTCTT CTGCTTGTCG GCCATGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA AGCACTGCAC GCCGTAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGTGGC GACCGGTAGC GCTAGGACGC GCGCGTAAGC TTAGGCCTTC GAAGATCCCG TAGTTTAAAC CTCGAGGCTA GTCTCGTGAT CGGAAAATTT TGAATTTTTG TAATTTGTTT TTGTAATTCT TTAGTTTGTA TGTCTGTTGC TATTATGTCT ACTATTCTTT CCCCTGCACT GTACCCCCCA ATCCCCCCTT TTCTTTTAAA AGTTAACCGA TACCGTCGAG ATTAATTAAA TTTAAATGTC GAAATTCCTC ACGACACCTG AAATGGAAGA AAAAAACTTT GAACCACTGT CTGAGGCTTG AGAATGAACC AAGATCCAAA CTCAAAAAGG GCAAATTCCA AGGAGAATTA CATCAAGTGC CAAGCTGGCC TAACTTCAGT CTCCACCCAC TCAGTGTGGG GAAACTCCAT CGCATAAAAC CCCTCCCCCC AACCTAAAGA CGACGTACTC CAAAAGCTCG AGAACTAATC GAGGTGCCTG GACGGCGCCC GGTACTCCGT GGAGTCACAT GAAGCGACGG CTGAGGACGG AAAGGCCCTT TTCCTTTGTG TGGGTGACTC ACCCGCCCGC TCTCCCGAGC GCCGCGTCCT CCATTTTGAG CTCCCTGCAG CAGGGCCGGG AAGCGGCCAT CTTTCCGCTC ACGCAACTGG TGCCGACCGG GCCAGCCTTG CCGCCCAGGG CGGGGCGATA CACGGCGGCG CGAGGCCAGG CACCAGAGCA GGCCGGCCAG CTTGAGACTA CCCCCGTCCG ATTCTCGGTG GCCGCGCTCG CAGGCCCCGC CTCGCCGAAC ATGTGCGCTG GGACGCACGG GCCCCGTCGC CGCCCGCGGC CCCAAAAACC GAAATACCAG TGTGCAGATC TTGGCCCGCA TTTACAAGAC TATCTTGCCA GAAAAAAAGC GTCGCAGCAG GTCATCAAAA ATTTTAAATG GCTAGAGACT TATCGAAAGC AGCGAGACAG GCGCGAAGGT GCCACCAGAT TCGCACGCGG CGGCCCCAGC GCCCAGGCCA GGCCTCAACT CAAGCACGAG GCGAAGGGGC TCCTTAAGCG CAAGGCCTCG AACTCTCCCA CCCACTTCCA ACCCGAAGCT CGGGATCAAG AATCACGTAC TGCAGCCAGT GGAAGTAATT CAAGGCACGC AAGGGCCATA ACCCGTAAAG AGGCCAGGCC CGCGGGAACC ACACACGGCA CTTACCTGTG TTCTGGCGGC AAACCCGTTG CGAAAAAGAA CGTTCACGGC GACTACTGCA CTTATATACG GTTCTCCCCC ACCCTCGGGA AAAAGGCGGA GCCAGTACAC GACATCACTT TCCCAGTTTA CCCCGCGCCA CCTTCTCTAG GCACCGGTTC AATTGCCGAC CCCTCCCCCC AACTTCTCGG GGACTGTGGG CGATGTGCGC TCTGCCCACT GACGGGCACC GGAGCCAATT CCCACTCCTT TCAAGACCTA GAAGGTCCAT TAGCTGCAAA GATTCCTCTC TGTTTAAAAC TTTATCCATC TTTGCAAAGC TTATCGTCGA CATCGCGATA CCGTCGAGAT CCGTTCACTA ATCGAATGGA TCTGTCTCTG TCTCTCTCTC CACCTTCTTC TTCTATTCCT TCGGGCCTGT CGGGTCCCCT CGGGGTTGGG AGGTGGGTCT GAAACGATAA TGGTGAATAT CCCTGCCTAA CTCTATTCAC TATAGAAAGT ACAGCAAAAA CTATTCTTAA ACCTACCAAG CCTCCTACTA TCATTATGAA TAATTTTATA TACCACAGCC AATTTGTTAT GTTAAACCAA TTCCACAAAC TTGCCCATTT ATCTAATTCC AATAATTCTT GTTCATTCTT TTCTTGCTGG TTTTGCGATT CTTCAATTAA GGAGTGTATT AAGCTTGTGT AATTGTTAAT TTCTCTGTCC CACTCCATCC AGGTCGTGTG ATTCCAAATC TGTTCCAGAG ATTTATTACT CCAACTAGCA TTCCAAGGCA CAGCAGTGGT GCAAATGAGT TTTCCAGAGC AACCCCAAAT CCCCAGGAGC TGTTGATCCT TTAGGTATCT TTCCACAGCC AGGATTCTTG CCTGGAGCTG CTTGATGCCC CAGACTGTGA GTTGCAACAG ATGCTGTTGC GCCTCAATAG CCCTCAGCAA ATTGTTCTGC TGCTGCACTA TACCAGACAA TAATTGTCTG GCCTGTACCG TCAGCGTCAT TGACGCTGCG CCCATAGTGC TTCCTGCTGC TCCCAAGAAC CCAAGGAACA AAGCTCCTAT TCCCACTGCT CTTTTTTCTC TCTGCACCAC TCTTCTCTTT GCCTTGGTGG GTGCTACTCC TAATGGTTCA ATTTTTACTA CTTTATATTT ATATAATTCA CTTCTCCAAT TGTCCCTCAT ATCTCCTCCT CCAGGTCTGA AGATCAGCGG CCGCTTGCTG TGCGGTGGTC TTACTTTTGT TTTGCTCTTC CTCTATCTTG TCTAAAGCTT CCTTGGTGTC TTTTATCTCT ATCCTTTGAT GCACACAATA GAGGGTTGCT ACTGTATTAT ATAATGATCT AAGTTCTTCT GATCCTGTCT GAAGGGATGG TTGTAGCTGT CCCAGTATTT GTCTACAGCC TTCTGATGTT TCTAACAGGC CAGGATTAAC TGCGAATCGT TCTAGCTCCC TGCTTGCCCA TACTATATGT TTTAATTTAT ATTTTTTCTT TCCCCCTGGC CTTAACCGAA TTTTTTCCCA TCGCGATCTA ATTCTCCCCC GCTTAATACT GACGCTCTCG CACCCATCTC TCTCCTTCTA GCCTCCGCTA GTCAAAATTT TTGGCGTACT CACCAGTCGC CGCCCCTCGC CTCTTGCCGT GCGCGCTTCA GCAAGCCGAG TCCTGCGTCG AGAGAGCTCC TCTGGTTTCC CTTTCGCTTT CAAGTCCCTG TTCGGGCGCC ACTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG GCAAAAAGCA GCTGCTTATA TGCAGGATCT GAGGGCTCGC CACTCCCCAG TCCCGCCCAG GCCACGCCTC CCTGGAAAGT CCCCAGCGGA AAGTCCCTTG TAGCAAGCTC GATATCAGCA GTTCTTGAAG TACTCCGGAT GCAGCTCTCG GGCCACGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAACACTT CTCTCTCCGG GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCTG GTGTTCTCTC CTTTATTGGC CTCTTCTACC TTATCTGGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG ATATCTGACC CCTGGCCCTG GTGTGTAGTT CTGCTAATCA GGGAAGTAGC CTTGTGTGTG GTAGATCCAC AGATCAAGGA TATCTTGTCT TCTTTGGGAG TGAATTAGCC CTTCCAA
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