pYX212
$128.00
In stock
SKU
PL82282
100μg liquid plasmid(Instantly):$149
| name | pYX212 | another name | apYX212 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | URA3 | Fluorescent labeling | |
| Promoter | TPI | Replicon | 2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAATTCACCA TGGATCCTAG GGCCCACAAG CTTACGCGTC GACCCGGGTA TCCGTATGAT GTGCCTGACT ACGCATGATA TCTCGAGCTC AGCTAGCTAA CTGAATAAGG AACAATGAAC GTTTTTCCTT TCTCTTGTTC CTAGTATTAA TGACTGACCG ATACATCCCT TTTTTTTTTT GTCTTTGTCT AGCTCCAGCT TTTGTTCCCT TTAGTGAGGG TTAATTCAAT TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGGTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT AGGGTAATAA CTGATATAAT TAAATTGAAG CTCTAATTTG TGAGTTTAGT ATACATGCAT TTACTTATAA TACAGTTTTT TAGTTTTGCT GGCCGCATCT TCTCAAATAT GCTTCCCAGC CTGCTTTTCT GTAACGTTCA CCCTGTACCT TAGCATCCCT TCCCTTTGCA AATAGTCCTC TTCCAACAAT AATAATGTCA GATCCTGTAG AGACCACATC ATCCACGGTT CTATACTGTT GACCCAATGC GTCTCCCTTG TCATCTAAAC CCACACCGGG TGTCATAATC AACCAATCGT AACCTTCATC TCTTCCACCC ATGTCTCTTT GAGCAATAAA GCCGATAACA AAATCTTTGT CGCTCTTCGC AATGTCAACA GTACCCTTAG TATATTCTCC AGTAGATAGG GAGCCCTTGC ATGACAATTC TGCTAACATC AAAAGGCCTC TAGGTTCCTT TGTTACTTCT TCTGCCGCCT GCTTCAAACC GCTAACAATA CCTGGCCCCA GCACACCGTG TGCATTCGTA ATGTCTGCCC ATTCTGCTAT TCTGTATACA CCCGCAGAGT ACTGCAATTT GACTGTATTA CCAATGTCAG CAAATTTTCT GTCTTCGAAG AGTAAAAAAT TGTACTTGGC GGATAATGCC TTTAGCGGCT TAACTGTGCC CTCCATCGAA AAATCAGTCA ATATATCCAC ATGTGTTTTT AGTAAACAAA TTTTGGGACC TAATGCTTCA ACTAACTCCA GTAATTCCTT GGTGGTACGA ACATCCAATG AAGCACACAA GTTTGTTTGC TTTTCGTGCA TGATATTAAA TAGCTTGGCA GCAACAGGAC TAGGATGAGT AGCAGCACGT TCCTTATATG TAGCTTTCGA CATGATTTAT CTTCGTTTCC TGCAGGTTTT TGTTCTGTGC AGTTGGGTTA AGAATACTGG GCAATTTCAT GTTTCTTCAA CACTACATAT GCGTATATAT ACCAATCTAA GTCTGTGCTC CTTCCTTCGT TCTTCCTTCT GTTCGGAGAT TACCGAATCA AAAAAATTTC AAAGAAACCG AAATCAAAAA AAAGAATAAA AAAAAAATGA TGAATTGAAT TGAAAAGCTG TGGTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTGC GGCCGCTCTA GAACTAGTGG ATCAATTCCA CGGACTATAG ACTATACTAG TATACTCCGT CTACTGTACG ATACACTTCC GCTCAGGTCC TTGTCCTTTA ACGAGGCCTT ACCACTCTTT TGTTACTCTA TTGATCCAGC TCAGCAAAGG CAGTGTGATC TAAGATTCTA TCTTCGCGAT GTAGTAAAAC TAGCTAGACC GAGAAAGAGA CTAGAAATGC AAAAGGCACT TCTACAATGG CTGCCATCAT TATTATCCGA TGTGACGCTG CAGCTTCTCA ATGATATTCG AATACGCTTT GAGGAGATAC AGCCTAATAT CCGACAAACT GTTTTACAGA TTTACGATCG TACTTGTTAC CCATCATTGA ATTTTGAACA TCCGAACCTG GGAGTTTTCC CTGAAACAGA TAGTATATTT GAACCTGTAT AATAATATAT AGTCTAGCGC TTTACGGAAG ACAATGTATG TATTTCGGTT CCTGGAGAAA CTATTGCATC TATTGCATAG GTAATCTTGC ACGTCGCATC CCCGGTTCAT TTTCTGCGTT TCCATCTTGC ACTTCAATAG CATATCTTTG TTAACGAAGC ATCTGTGCTT CATTTTGTAG AACAAAAATG CAACGCGAGA GCGCTAATTT TTCAAACAAA GAATCTGAGC TGCATTTTTA CAGAACAGAA ATGCAACGCG AAAGCGCTAT TTTACCAACG AAGAATCTGT GCTTCATTTT TGTAAAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA ACAAAGAATC TGAGCTGCAT TTTTACAGAA CAGAAATGCA ACGCGAGAGC GCTATTTTAC CAACAAAGAA TCTATACTTC TTTTTTGTTC TACAAAAATG CATCCCGAGA GCGCTATTTT TCTAACAAAG CATCTTAGAT TACTTTTTTT CTCCTTTGTG CGCTCTATAA TGCAGTCTCT TGATAACTTT TTGCACTGTA GGTCCGTTAA GGTTAGAAGA AGGCTACTTT GGTGTCTATT TTCTCTTCCA TAAAAAAAGC CTGACTCCAC TTCCCGCGTT TACTGATTAC TAGCGAAGCT GCGGGTGCAT TTTTTCAAGA TAAAGGCATC CCCGATTATA TTCTATACCG ATGTGGATTC CGCATACTTT GTGAACAGAA AGTGATAGCG TTGATGATTC TTCATTGGTC AGAAAATTAT GAACGGTTTC TTCTATTTTG TCTCTATATA CTACGTATAG GAAATGTTTA CATTTTCGTA TTGTTTTCGA TTCACTCTAT GAATAGTTCT TACTACAATT TTTTTGTCTA AAGAGTAATA CTAGAGATAA ACATAAAAAA TGTAGAGGTC GAGTTTAGAT GCAAGTTCAA GGAGCGAAAG GTGGATGGGT AGGTTATATA GGGATATAGC ACAGAGATAT ATAGCAAAGA GATACTTTTC AGCAATGTTT GTGGAAGCGG TATTCGCAAT ATTTTAGTAG CTCGTTACAG TCCGGTGCGT TTTTGGTTTT TTGAAAGTGC GTCTTCAGAG CGCTTTTGGT TTTCAAAAGC GCTCTGAAGT TCCTATACTT TCTAGAGAAT AGGAACTTCG GAATAGGAAC TTCAAAGCGT TTCCGAAAAC GAGCGCTTCC GAAAATGCAA CGCGAGCTGC GCACATACAG CTCACTGTTC ACGTCGCACC TATATCTGCG TGTTGCCTGT ATATATATAT ACATGAGAAG AACGGCATAG TGCGTGTTTA TGCTTAAATG CGTACTTATA TGCGTCTATT TATGTAGGAT GAAAGGTAGT CTAGTACCTC CTGTGATATT ATCCCATTCC ATGCGGGGTA TCGTATGCTT CCTTCAGCAC TACCCTTTAG CTGTTCTATA TGCTGCCACT CCTCAATTGG ATTAGTCTCA TCCTTCAATG CTATCATTTC CTTTGATATT GGATCATATG CATAGTACCG AGAAACTAGT GCGAAGTAGT GATCAGGTAT TGCTGTTATC TGATGAGTAT ACGTTGTCCT GGCCACGGCA GAAGCACCCT TATCGCTCCA ATTTCCCACA ACATTAGTCA ACTCCGTTAG GCCCTTCATT GAAAGAAATG AGGTCATCAA ATGTCTTCCA ATGTGAGATT TTGGGCCATT TTTTATAGCA AAGATTGAAT AAGGCGCATT TTTCTTCAAA GCTGCGGCCG CACTCTCACT AGTACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CGTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGCTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGGA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGGTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTACCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCCTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTCGA AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCGGC CGTCGAGCTT GATGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGAAAAA AAGCGGTTAG CTCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGGAA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTTCAAGAA TTGGGGATCT ACGTATGGTC ATTCTTCTTC AGATTCCCTC ATGGAGAAGT GCGGCAGATG TATATGACAG AGTCGCCAGT TTCCAAGAGA CTTTATTCAG GCACTTCCAT GATAGGCAAG AGAGAAGACC CAGAGATGTT GTTGTCCTAG TTACACATGG TATTTATTCC AGAGTATTCC TGATGAAATG GTTTAGATGG ACATACGAAG AGTTTGAATC GTTTACCAAT GTTCCTAACG GGAGCGTAAT GGTGATGGAA CTGGACGAAT CCATCAATAG ATACGTCCTG AGGACCGTGC TACCCAAATG GACTGATTGT GAGGGAGACC TAACTACATA GTGTTTAAAG ATTACGGATA TTTAACTTAC TTAGAATAAT GCCATTTTTT TGAGTTATAA TAATCCTACG TTAGTGTGAG CGGGATTTAA ACTGTGAGGA CCTCAATACA TTCAGACACT TCTGACGGTA TCACCCTACT TATTCCCTTC GAGATTATAT CTAGGAACCC ATCAGGTTGG TGGAAGATTA CCCGTTCTAA GACTTTTCAG CTTCCTCTAT TGATGTTACA CTCGGACACC CCTTTTCTGG CATCCAGTTT TTAATCTTCA GTGGCATGTG AGATTCTCCG AAATTAATTA AAGCAATCAC ACAATTCTCT CGGATACCAC CTCGGTTGAA ACTGACAGGT GGTTTGTTAC GCATGCTAAT GCAAAGGAGC CTATATACCT TTGGCTCGGC TGCTGTAACA GGGAATATAA AGGGCAGCAT AATTTAGGAG TTTAGTGAAC TTGCAACATT TACTATTTTC CCTTCTTACG TAAATATTTT TCTTTTTAAT TCTAAATCAA TCTTTTTCAA TTTTTTGTTT GTATTCTTTT CTTGCTTAAA TCTATAACTA CAAAAAACAC ATACAG
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