pKLAC1
$128.00
In stock
SKU
PL82276
100μg liquid plasmid(Instantly):$149
| name | pKLAC1 | another name | apKLAC1 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AAGCTTGAAA AAAATGAAAT TCTCTACTAT ATTAGCCGCA TCTACTGCTT TAATTTCCGT TGTTATGGCT GCTCCAGTTT CTACCGAAAC TGACATCGAC GATCTTCCAA TATCGGTTCC AGAAGAAGCC TTGATTGGAT TCATTGACTT AACCGGGGAT GAAGTTTCCT TGTTGCCTGT TAATAACGGA ACCCACACTG GTATTCTATT CTTAAACACC ACCATCGCTG AAGCTGCTTT CGCTGACAAG GATGATCTCG AGAAAAGAGA GGCTGAAGCT AGAAGAGCTA GATCTCCTAG GGGTACCGTC GACGGCGCGC CTGCGGCCGC TTAATTAAGG CCTTGAATCG AGAATTTATA CTTAGATAAG TATGTACTTA CAGGTATATT TCTATGAGAT ACTGATGTAT ACATGCATGA TAATATTTAA ACGGTTATTA GTGCCGATTG TCTTGTGCGA TAATGACGTT CCTATCAAAG CAATACACTT ACCACCTATT ACATGGGCCA AGAAAATATT TTCGAACTTG TTTAGAATAT TAGCACAGAG TATATGATGA TATCCGTTAG ATTATGCATG ATTCATTCCT ACAACTTTTT CGTAGCATAA GGATTAATTA CTTGGATGCC AATAAAAAAA AAAAACATCG AGAAAATTTC AGCATGCTCA GAAACAATTG CAGTGTATCA AAGTAAAAAA AAGATTTTCA CTACATGTTC CTTTTGAAGA AAGAAAATCA TGGAACATTA GATTTACAAA AATTTAACCA CCGCTGATTA ACGATTAGAC CGTTAAGCGC ACAACAGGTT ATTAGTACAG AGAAAGCATT CTGTGGTGTT GCCCCGGACT TTCTTTTGCG ACATAGGTAA ATCGAATACC ATCATACTAT CTTTTCCAAT GACTCCCTAA AGAAAGACTC TTCTTCGATG TTGTATACGT TGGAGCATAG GGCAAGAATT GTGGCTTGAG ATCATCCTTT TGTTGTTTCC GGGTGTACAA TATGGACTTC CTCTTTTCTG GCAACCAAAC CCATACATCG GGATTCCTAT AATACCTTCG TTGGTCTCCC TAACATGTAG GTGGCGGAGG GGAGATATAC AATAGAACAG ATACCAGACA AGACATAATG GGCTAAACAA GACTACACCA ATTACACTGC CTCATTGATG GTGGTACATA ACGAACTAAT ACTGTAGCCC TAGACTTGAT AGCCATCATC ATATCGAAGT TTCACTACCC TTTTTCCATT TGCCATCTAT TGAAGTAATA ATAGGCGCAT GCAACTTCTT TTCTTTTTTT TTCTTTTCTC TCTCCCCCGT TGTTGTCTCA CCATATCCGC AATGACAAAA AAATGATGGA AGACACTAAA GGAAAAAATT AACGACAAAG ACAGCACCAA CAGATGTCGT TGTTCCAGAG CTGATGAGGG GTATCTCGAA GCACACGAAA CTTTTTCCTT CCTTCATTCA CGCACACTAC TCTCTAATGA GCAACGGTAT ACGGCCTTCC TTCCAGTTAC TTGAATTTGA AATAAAAAAA AGTTTGCTGT CTTGCTATCA AGTATAAATA GACCTGCAAT TATTAATCTT TTGTTTCCTC GTCATTGTTC TCGTTCCCTT TCTTCCTTGT TTCTTTTTCT GCACAATATT TCAAGCTATA CCAAGCATAC AATCAAGGAA TTCCGGATCC GCCACCATGC CTCAATCCTG GGAAGAACTG GCCGCTGATA AGCGCGCCCG CCTCGCAAAA ACCATCCCTG ATGAATGGAA AGTCCAGACG CTGCCTGCGG AAGACAGCGT TATTGATTTC CCAAAGAAAT CGGGGATCCT TTCAGAGGCC GAACTGAAGA TCACAGAGGC TTCCGCTGCG GATCTTGTGT CCAAGCTGGC GGCCGGAGAG TTGACCTCGG TGGAAGTTAC GCTAGCATTC TGTAAACGGG CAGCAATCGC CCAGCAGTTA ACAAACTGCG CCCACGAGTT CTTCCCTGAC GCCGCTCTCG CGCAGGCAAG GGAACTCGAT GAATACTACG CAAAGCACAA GAGACCCGTT GGTCCACTCC ATGGCCTCCC CATCTCTCTC AAAGACCAGC TTCGAGTCAA GGGCTACGAA ACATCAATGG GCTACATCTC ATGGCTAAAC AAGTACGACG AAGGGGACTC GGTTCTGACA ACCATGCTCC GCAAAGCCGG TGCCGTCTTC TACGTCAAGA CCTCTGTCCC GCAGACCCTG ATGGTCTGCG AGACAGTCAA CAACATCATC GGGCGCACCG TCAACCCACG CAACAAGAAC TGGTCGTGCG GCGGCAGTTC TGGTGGTGAG GGTGCGATCG TTGGGATTCG TGGTGGCGTC ATCGGTGTAG GAACGGATAT CGGTGGCTCG ATTCGAGTGC CGGCCGCGTT CAACTTCCTG TACGGTCTAA GGCCGAGTCA TGGGCGGCTG CCGTATGCAA AGATGGCGAA CAGCATGGAG GGTCAGGAGA CGGTGCACAG CGTTGTCGGG CCGATTACGC ACTCTGTTGA GGACCTCCGC CTCTTCACCA AATCCGTCCT CGGTCAGGAG CCATGGAAAT ACGACTCCAA GGTCATCCCC ATGCCCTGGC GCCAGTCCGA GTCGGACATT ATTGCCTCCA AGATCAAGAA CGGCGGGCTC AATATCGGCT ACTACAACTT CGACGGCAAT GTCCTTCCAC ACCCTCCTAT CCTGCGCGGC GTGGAAACTA CCGTCGCCGC ACTCGCCAAA GCCGGTCACA CCGTGACCCC GTGGACGCCA TACAAGCACG ATTTCGGCCA CGATCTCATC TCCCATATCT ACGCGGCTGA CGGCAGCGCC GACGTAATGC GCGATATCAG TGCATCCGGC GAGCCGGCGA TTCCAAATAT CAAAGACCTA CTGAACCCGA ACATCAAAGC TGTTAACATG AACGAGCTCT GGGACACGCA TCTCCAGAAG TGGAATTACC AGATGGAGTA CCTTGAGAAA TGGCGGGAGG CTGAAGAAAA GGCCGGGAAG GAACTGGACG CCATCATCGC GCCGATTACG CCTACCGCTG CGGTACGGCA TGACCAGTTC CGGTACTATG GGTATGCCTC TGTGATCAAC CTGCTGGATT TCACGAGCGT GGTTGTTCCG GTTACCTTTG CGGATAAGAA CATCGATAAG AAGAATGAGA GTTTCAAGGC GGTTAGTGAG CTTGATGCCC TCGTGCAGGA AGAGTATGAT CCGGAGGCGT ACCATGGGGC ACCGGTTGCA GTGCAGGTTA TCGGACGGAG ACTCAGTGAA GAGAGGACGT TGGCGATTGC AGAGGAAGTG GGGAAGTTGC TGGGAAATGT GGTGACTCCA TAGCCCGGGG GGGGCTCGAT CCCCTCGCGA GTTGGTTCAG CTGCTGCCTG AGGCTGGACG ACCTCGCGGA GTTCTACCGG CAGTGCAAAT CCGTCGGCAT CCAGGAAACC AGCAGCGGCT ATCCGCGCAT CCATGCCCCC GAACTGCAGG AGTGGGGAGG CACGATGGCC GCTTTGGTCG ATCTAGATTA CGTGGAAGAA AGGTAGTAAA AGTAGTAGTA TAAGTAGTAA AAAGAGGTAA AAAGAGAAAA CCGGCTACAT ACTAGAGAAG CACGTACACA AAAACTCATA GGCACTTCAT CATACGACAG TTTCTTGATG CATTATAATA GTGTATTAGA TATTTTCAGA AATATGCATA GAACCTCCTC TTGCCTTTAC TTTTTATACA TAGAACATTG GCAGATTTAC TTACACTACT TTGTTTCTAC GCCATTTCTT TTGTTTTCAA CACTTAGACA AGTTGTTGAG AACCGGACTA CTAAAAAGCA ATGTTCCCAC TGAAAATCAT GTACCTGCAG GATAATAACC CCCTAATTCT GCATCGATCC AGTATGTTTT TTTTTCTCTA CTCATTTTTA CCTGAAGATA GAGCTTCTAA AACAAAAAAA ATCAGTGATT ACATGCATAT TGTGTGTTCT AGTAACCAAA GGAAAGGAAC AGATAGATAA AATTCCGAGA CTGTCAAATT AGGTTTTTTT CTTTTTTTTT GGCGGGAGTC AGTGGGCCGA AATATGTTCT TGGCCTAGAA CTTAATCTGG TTTGATCATG CCAATACTTG CCTGAGTGCC CGACTTTTTG CCCACCCTCT TGCCTTCTGT CATCCTTCAA AACCCACCTG TTTTCCAGCC GTATCTTCGC TCGCATCTAC ACATACTGTG CCATATCTTG TGTGTAGCCG GACGTGACTA TGACCAAAAA CAAACAAGGA GAACTGTTCG CCGATTTGTA ACACTCCTGC ATCCATCCAA GTGGGTATGC GCTATGCAAT GTTAAGCTAG GTCAGGTCAG ACCAGGTCCA AGGACAGCAA CTTGACTGTA TGCAACCTTT ACCATCTTTG CACAGAACAT ACTTGTAGCT AGCTAGTTAC ACTTATGGAC CGAAAAGGCA CCCCACCATG TCTGTCCGGC TTTAGAGTAC GGCCGCAGAC CGCTGATTTG CCTTGCCAAG CAGTAGTCAC AATGCATCGC ATGAGCACAC GGGCACGGGC ACGGGCACAG GAACCATTGG CAAAAATACC AGATACACTA TACCGACGTA TATCAAGCCC AAGTTTAAAA TTCCTAAATT TCCGCGGCTA CTTTTCAATT CCCTATAGTG AGTCGTATTA AATTCGTAAT CATGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG CCAAGCTCCC GCGGGGATCG ACTCATAAAA TAGTAACCTT CTAATGCGTA TCTATTGACT ACCAACCATT AGTGTGGTTG CAGAAGGCGG AATTCTCCCT TCTTCGAATT CAGCTTGCTT TTTCATTTTT TATTTTCCAT TTTTCAGTTT TTGTTTGTGT CGAATTTAGC CAGTTGCTTC TCCAAGATGA AAAAAACCCC TGCGCAGTTT CTGTGCTGCA AGATCCTAAT CGACTTTTCC ACCCCCCACA AAAGTAAATG TTCTTTTGTT ACATTCGCGT GGGTAGCTAG CTCCCCGAAT CTTCAAAGGA CTTAGGGACT GCACTACATC AGAGTGTGTT CACCTGGTTT GCTGCCTGGT TTGAAAGAAA AGAGCAGGGA ACTCGCGGGT TCCCGGCGAA TAATCATGCG ATAGTCCTTT GGCCTTCCAA GTCGCATGTA GAGTAGACAA CAGACAGGGA GGGCAGGAAG GATCTTTCAC TGAGATCCTG TATCTTGTTG GGTAAGTCGG ATGAAAGGGG AATCGTATGA GATTGGAGAG GATGCGGAAG AGGTAACGCC TTTTGTTAAC TTGTTTAATT ATTATGGGGC AGGCGAGAGG GGGAGGAATG TATGTGTGTG AGGCGGGCGA GACGGAGCCA TCCAGGCCAG GTAGAAATAG AGAAAGCCGA ATGTTAGACA ATATGGCAGC GTAGTAGAGT AGGTAGGTAG GCAAGTACTG CTAGCAAAGA GGAGAAGGGT AAGCTCACTC TTCGCATTCC ACACCGTTAG TGTGTCAGTT TGAACAAAAA AACAATCATC ATACCAATTG ATGGACTGTG GACTGGCTTT TGGAACGGCT TTTCGGACTG CGATTATTCG TGAGGAATCA AGGTAGGAAT TTGGTCATAT TTACGGACAA CAGTGGGTGA TTCCCATATG GAGTAGGAAA ACGAGATCAT GGTATCCTCA GATATGTTGC GGAATTCTGT TCACCGCAAA GTTCAGGGTG CTCTGGTGGG TTTCGGTTGG TCTTTGCTTT GCTTCTCCCT TGTCTTGCAT GTTAATAATA GCCTAGCCTG TGAGCCGAAA CTTAGGGTAG GCTTAGTGTT GGAACGTACA TATGTATCAC GTTGACTTGG TTTAACCAGG CGACCTGGTA GCCAGCCATA CCCACACACG TTTTTTGTAT CTTCAGTATA GTTGTGAAAA GTGTAGCGGA AATTTGTGGT CCGAGCAACA GCGTCTTTTT CTAGTAGTGC GGTCGGTTAC TTGGTTGACA TTGGTATTTG GACTTTGTTG CTACACCATT CACTACTTGA AGTCGAGTGT GAAGGGTATG ATTTCTAGTG GTGAACACCT TTAGTTACGT AATGTTTTCA TTGCTGTTTT ACTTGAGATT TCGATTGAGA AAAAGGTATT TAATAGCTCG AATCAATGTG AGAACAGAGA GAAGATGTTC TTCCCTAACT CGAAAGGTAT ATGAGGCTTG TGTTTCTTAG GAGAATTATT ATTCTTTTGT TATGTTGCGC TTGTAGTTGG AAAAGGTGAA GAGACAAAAG CTGGAATTGT GAGCGGATAA CAAGCTCAAC ACTTGAAATT TAGGAAAGAG CAGAATTTGG CAAAAAAAAT AAAAAAAAAA TAAACACACA TACTCATCGA G
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