pYD1-LOC9269328(136-2051)(rice)
$99.00
In stock
SKU
PL82270
XM_015790705.2
100μg liquid plasmid(Instantly):$120
| name | pYD1-LOC9269328(136-2051)(rice) | another name | apYD1-LOC9269328(136-2051)(rice) |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression,Secretion expression | Fragment type | ORF |
| Fragment species | Rice | Prokaryotic resistance | Amp |
| Selective marker | TRP1 | Fluorescent labeling | |
| Promoter | GAL1 | Replicon | CEN/ARS |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC GACTCACTAT AGGGAATATT AAGCTAATTC TACTTCATAC ATTTTCAATT AAGATGCAGT TACTTCGCTG TTTTTCAATA TTTTCTGTTA TTGCTTCAGT TTTAGCACAG GAACTGACAA CTATATGCGA GCAAATCCCC TCACCAACTT TAGAATCGAC GCCGTACTCT TTGTCAACGA CTACTATTTT GGCCAACGGG AAGGCAATGC AAGGAGTTTT TGAATATTAC AAATCAGTAA CGTTTGTCAG TAATTGCGGT TCTCACCCCT CAACGACTAG CAAAGGCAGC CCCATAAACA CACAGTATGT TTTTAAGCTT CTGCAGGCTA GTGGTGGTGG TGGTTCTGGT GGTGGTGGTT CTGGTGGTGG TGGTTCTGCT AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG ACGATGACGA TAAGGTACCA GGATCCATGC TCGACAACGG GCTCGGGCGG ACGCCGCAGA TGGGGTGGAA CAGTTGGAAT CATTTCGGGT GCAACATCAA CGAGAACACC ATCCGGAGCA CCGTTGACGC ACTCATCTCC ACCGGGCTCA CGAAGGCTGG ATACACTTAT GTCAACTTAG ATGATTGCTG GGCAGATTAC CAAAGGACCA AAGAGGGTTA CATAGCTGCT GATCCAAAGA AGTTCCCTTC GGGGATTAAG GCACTCGCAG ATTATGTGCA CAGCAAGGGG CTTAAACTAG GCATATACTC TTCAGCCGGC TCAAGAACTT GCAGCAAAAC GATGCCCGGA TCTCTGGGCT ACGAGGACAT CGATGCTAAG ACATTTGCAT CATGGGGTGT GGATTACTTG AAGTACGACA ACTGCAACAG TGACGGCTCG AGCGAAACCG TCAGATTCCC CAAGATGTCT TTCGCTCTGA GAAAAACCGG ACGTCCGATC TTCTACTCGA TCTGCGAATG GGGCCAGAGG AATGTGGCGA CGTGGGGTGG CCAGTACGGC AACAGCTGGA GGACCACCGG CGACATCAAT GACTCATGGG CCAGCATGCT GAGCAACATC GACAGCAACG ACGCCTCCGC CAGCTACGCC AAGCCCGGCG GCTGGAACGA TCCTGACATG CTGGAGGTCG GCAATGGAGG GATGACGAAC GACGAGTACG TCGTGCACAT CAGCCTTTGG GCGATTGCCA AGGCTCCTCT GATCATCGGG TGCGACGTGA GGAGTATCTC CAGGGAGACG ATGGAGATCC TCTCCAACCC TGAAGTCATC GCCATTAACC AAGATCCATT GGGCGTCCAG GGAAAGAAAG TCAGGAAGTA CGACAACGAA ATCGAGGTAT GGGCAGGGCC GCTCTCCCAG CAGAGGACGG CGGTGCTGCT GCTGAACCGC GGCGCCACGG GCAGCCGGCA GATCACGGCG GCGTGGCAGG ACATCGGCGT CGGCCCCGGC GTCGCCGTCG AGGCGAAGAA CGTCTGGCTG CACGCGACGG CGCCGGGGAG GTTCACCGGC AGCCTGACGG CGGAGGTGGC GGCGCACTCG TGCAAGCTCT TCGTCCTCAC GCCCGTCGGC CGGGCCAGCG AGGAGCGGAG CGAATTCTGC AGATATCCAG CACAGTGGCG GCCGCTCGAG TCTAGAGGGC CCTTCGAAGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG CGTACCGGTC ATCATCACCA TCACCATTGA GTTTAAACCC GCTGATCTGA TAACAACAGT GTAGATGTAA CAAAATCGAC TTTGTTCCCA CTGTACTTTT AGCTCGTACA AAATACAATA TACTTTTCAT TTCTCCGTAA ACAACATGTT TTCCCATGTA ATATCCTTTT CTATTTTTCG TTCCGTTACC AACTTTACAC ATACTTTATA TAGCTATTCA CTTCTATACA CTAAAAAACT AAGACAATTT TAATTTTGCT GCCTGCCATA TTTCAATTTG TTATAAATTC CTATAATTTA TCCTATTAGT AGCTAAAAAA AGATGAATGT GAATCGAATC CTAAGAGAAT TGGGCAAGTG CACAAACAAT ACTTAAATAA ATACTACTCA GTAATAACCT ATTTCTTAGC ATTTTTGACG AAATTTGCTA TTTTGTTAGA GTCTTTTACA CCATTTGTCT CCACACCTCC GCTTACATCA ACACCAATAA CGCCATTTAA TCTAAGCGCA TCACCAACAT TTTCTGGCGT CAGTCCACCA GCTAACATAA AATGTAAGCT CTCGGGGCTC TCTTGCCTTC CAACCCAGTC AGAAATCGAG TTCCAATCCA AAAGTTCACC TGTCCCACCT GCTTCTGAAT CAAACAAGGG AATAAACGAA TGAGGTTTCT GTGAAGCTGC ACTGAGTAGT ATGTTGCAGT CTTTTGGAAA TACGAGTCTT TTAATAACTG GCAAACCGAG GAACTCTTGG TATTCTTGCC ACGACTCATC TCCGTGCAGT TGGACGATAT CAATGCCGTA ATCATTGACC AGAGCCAAAA CATCCTCCTT AGGTTGATTA CGAAACACGC CAACCAAGTA TTTCGGAGTG CCTGAACTAT TTTTATATGC TTTTACAAGA CTTGAAATTT TCCTTGCAAT AACCGGGTCA ATTGTTCTCT TTCTATTGGG CACACATATA ATACCCAGCA AGTCAGCATC GGAATCTAGA GCACATTCTG CGGCCTCTGT GCTCTGCAAG CCGCAAACTT TCACCAATGG ACCAGAACTA CCTGTGAAAT TAATAACAGA CATACTCCAA GCTGCCTTTG TGTGCTTAAT CACGTATACT CACGTGCTCA ATAGTCACCA ATGCCCTCCC TCTTGGCCCT CTCCTTTTCT TTTTTCGACC GAATTTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGGAC GGATCGCTTG CCTGTAACTT ACACGCGCCT CGTATCTTTT AATGATGGAA TAATTTGGGA ATTTACTCTG TGTTTATTTA TTTTTATGTT TTGTATTTGG ATTTTAGAAA GTAAATAAAG AAGGTAGAAG AGTTACGGAA TGAAGAAAAA AAAATAAACA AAGGTTTAAA AAATTTCAAC AAAAAGCGTA CTTTACATAT ATATTTATTA GACAAGAAAA GCAGATTAAA TAGATATACA TTCGATTAAC GATAAGTAAA ATGTAAAATC ACAGGATTTT CGTGTGTGGT CTTCTACACA GACAAGATGA AACAATTCGG CATTAATACC TGAGAGCAGG AAGAGCAAGA TAAAAGGTAG TATTTGTTGG CGATCCCCCT AGAGTCTTTT ACATCTTCGG AAAACAAAAA CTATTTTTTC TTTAATTTCT TTTTTTACTT TCTATTTTTA ATTTATATAT TTATATTAAA AAATTTAAAT TATAATTATT TTTATAGCAC GTGATGAAAA GGACCCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGCAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGG AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCCGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTACCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCCTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTCGGAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG CTAGT
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