pVLT33
$157.00
In stock
SKU
PL82257
100μg liquid plasmid(Instantly):$192
| name | pVLT33 | another name | apVLT33 |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | Tac | Replicon | |
| Competence | EPI300 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGCTTTATGC TTGTAAACCG TTTTGTGAAA AAATTTTTAA AATAAAAAAG GGGACCTCTA GGGTCCCCAA TTAATTAGTA ATATAATCTA TTAAAGGTCA TTCAAAAGGT CATCCACCGG ATCAGCTTAG TAAAGCCCTC GCTAGATTTT AATGCGGATG TTGCGATTAC TTCGCCAACT ATTGCGATAA CAAGAAAAAG CCAGCCTTTC ATGATATATC TCCCAATTTG TGTAGGGCTT ATTATGCACG CTTAAAAATA ATAAAAGCAG ACTTGACCTG ATAGTTTGGC TGTGAGCAAT TATGTGCTTA GTGCATCTAA CGCTTGAGTT AAGCCGCGCC GCGAAGCGGC GTCGGCTTGA ACGAATTGTT AGACATTATT TGCCGACTAC CTTGGTGATC TCGCCTTTCA CGTAGTGGAC AAATTCTTCC AACTGATCTG CGCGAGCTTC ACGCTGCCGC AAGCACTCAG GGCGCAAGGG CTGCTAAAGG AAGCGGAACA CGTAGAAAGC CAGTCCGCAG AAACGGTGCT GACCCCGGAT GAATGTCAGC TACTGGGCTA TCTGGACAAG GGAAAACGCA AGCGCAAAGA GAAAGCAGGT AGCTTGCAGT GGGCTTACAT GGCGATAGCT AGACTGGGCG GTTTTATGGA CAGCAAGCGA ACCGGAATTG CCAGCTGGGG CGCCCTCTGG TAAGGTTGGG AAGCCCTGCA AAGTAAACTG GATGGCTTTC TTGCCGCCAA GGATCTGATG GCGCAGGGGA TCAAGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCGGG CTCGATCCCC TCGCGAGTTG GTTCAGCTGC TGCCTGAGGC TGGACGACCT CGCGGAGTTC TACCGGCAGT GCAAATCCGT CGGCATCCAG GAAACCAGCA GCGGCTATCC GCGCATCCAT GCCCCCGAAC TGCAGGAGTG GGGAGGCACG ATGGCCGCTT TGGTCGACGG ATCAGTGAGG GTTTGCAACT GCGGGTCAAG GATCTGGATT TCGATCACGG CACGATCATC GTGCGGGAGG GCAAGGGCTC CAAGGATCGG GCCTTGATGT TACCCGAGAG CTTGGCACCC AGCCTGCGCG AGCAGGGGAA TTGATCCGGT GGATGACCTT TTGAATGACC TTTAATAGAT TATATTACTA ATTAATTGGG GACCCTAGAG GTCCCCTTTT TTATTTTAAA AATTTTTTCA CAAAACGGTT TACAAGCATA AAGCTAAACG TGGCCAATAT GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT TATACGCAAG GCGACAAGGT GCTGATGCCG CTGGCGATTC AGGTTCATCA TGCCGTTTGT GATGGCTTCC ATGTCGGCAG AATGCTTAAT GAATTACAAC AGTACTGCGA TGAGTGGCAG GGCGGGGCGT AATTTTTTTA AGGCAGTTAT TGTCTTCAAA TTCCCGTTGC ACATAGCCCG GCAATTCCTT TCCCTGCTCT GCCATAAGCG CAGCGAATGC CGGGTAATAC TCGTCAACGA TCTGATAGAG AAGGGTTTGC TCGGGTCGGT GGCTCTGGTA ACGACCAGTA TCCCGATCCC GGCTGGCCGT CCTGGCCGCC ACATGAGGCA TGTTCCGCGT CCTTGCAATA CTGTGTTTAC ATACAGTCTA TCGCTTAGCG GAAAGTTCTT TTACCCTCAG CCGAAATGCC TGCCGTTGCT AGACATTGCC AGCCAGTGCC CGTCACTCCC GTACTAACTG TCACGAACCC CTGCAATAAC TGTCACGCCC CCCTGCAATA ACTGTCACGA ACCCCTGCAA TAACTGTCAC GCCCCCAAAC CTGCAAACCC AGCAGGGGCG GGGGCTGGCG GGGTGTTGGA AAAATCCATC CATGATTATC TAAGAATAAT CCACTAGGCG CGGTTATCAG CGCCCTTGTG GGGCGCTGCT GCCCTTGCCC AATATGCCCG GCCAGAGGCC GGATAGCTGG TCTATTCGCT GCGCTAGGCT ACACACCGCC CCACCGCTGC GCGGCAGGGG GAAAGGCGGG CAAAGCCCGC TAAACCCCAC ACCAAACCCC GCAGAAATAC GCTGGAGCGC TTTTAGCCGC TTTAGCGGCC TTTCCCCCTA CCCGAAGGGT GGGGGCGCGT GTGCAGCCCC GCAGGGCCTG TCTCGGTCGA TCATTCAGCC CGGCTCATCC TTCTGGCGTG GCGGCAGACC GAACAAGGCG CGGTCGTGGT CGCGTTCAAG GTACGCATCC ATTGCCGCCA TGAGCCGATC CTCCGGCCAC TCGCTGCTGT TCACCTTGGC CAAAATCATG GCCCCCACCA GCACCTTGCG CCTTGTTTCG TTCTTGCGCT CTTGCTGCTG TTCCCTTGCC CGCTCCCGCT GAATTTCGGC ATTGATTCGC GCTCGTTGTT CTTCGAGCTT GGCCAGCCGA TCCGCCGCCT TGTTGCTCCC CTTAACCATC TTGACACCCC ATTGTTAATG TGCTGTCTCG TAGGCTATCA TGGAGGCACA GCGGCGGCAA TCCCGACCCT ACTTTGTAGG GGAGGGCGCA CTTACCGGTT TCTCTTCGAG AAACTGGCCT AACGGCCACC CTTCGGGCGG TGCGCTCTCC GAGGGCCATT GCATGGAGCC GAAAAGCAAA AGCAACAGCG AGGCAGCATG GCGATTTATC ACCTTACGGC GAAAACCGGC AGCAGGTCGG GCGGCCAATC GGCCAGGGCC AAGGCCGACT ACATCCAGCG CGAAGGCAAG TATGCCCGCG ACATGGATGA AGTCTTGCAC GCCGAATCCG GGCACATGCC GGAGTTCGTC GAGCGGCCCG CCGACTACTG GGATGCTGCC GACCTGTATG AACGCGCCAA TGGGCGGCTG TTCAAGGAGG TCGAATTTGC CCTGCCGGTC GAGCTGACCC TCGACCAGCA GAAGGCGCTG GCGTCCGAGT TCGCCCAGCA CCTGACCGGT GCCGAGCGCC TGCCGTATAC GCTGGCCATC CATGCCGGTG GCGGCGAGAA CCCGCACTGC CACCTGATGA TCTCCGAGCG GATCAATGAC GGCATCGAGC GGCCCGCCGC TCAGTGGTTC AAGCGGTACA ACGGCAAGAC CCCGGAGAAG GGCGGGGCAC AGAAGACCGA AGCGCTCAAG CCCAAGGCAT GGCTTGAGCA GACCCGCGAG GCATGGGCCG ACCATGCCAA CCGGGCATTA GAGCGGGCTG GCCACGACGC CCGCATTGAC CACAGAACAC TTGAGGCGCA GGGCATCGAG CGCCTGCCCG GTGTTCACCT GGGGCCGAAC GTGGTGGAGA TGGAAGGCCG GGGCATCCGC ACCGACCGGG CAGACGTGGC CCTGAACATC GACACCGCCA ACGCCCAGAT CATCGACTTA CAGGAATACC GGGAGGCAAT AGACCATGAA CGCAATCGAC AGAGTGAAGA AATCCAGAGG CATCAACGAG TTAGCGGAGC AGATCGAACC GCTGGCCCAG AGCATGGCGA CACTGGCCGA CGAAGCCCGG CAGGTCATGA GCCAGACCAA GCAGGCCAGC GAGGCGCAGG CGGCGGAGTG GCTGAAAGCC CAGCGCCAGA CAGGGGCGGC ATGGGTGGAG CTGGCCAAAG AGTTGCGGGA GGTAGCCGCC GAGGTGAGCA GCGCCGCGCA GAGCGCCCGG AGCGCGTCGC GGGGGTGGCA CTGGAAGCTA TGGCTAACCG TGATGCTGGC TTCCATGATG CCTACGGTGG TGCTGCTGAT CGCATCGTTG CTCTTGCTCG ACCTGACGCC ACTGACAACC GAGGACGGCT CGATCTGGCT GCGCTTGGTG GCCCGATGAA GAACGACAGG ACTTTGCAGG CCATAGGCCG ACAGCTCAAG GCCATGGGCT GTGAGCGCTT CGATATCGGC GTCAGGGACG CACCCACCGG CCAGATGATG AACCGGGAAT GGTCAGCCGC CGAAGTGCTC CAGAACACGC CATGGCTCAA GCGGATGAAT GCCCAGGGCA ATGACGTGTA TATCAGGCCC GCCGAGCAGG AGCGGCATGG TCTGGTGCTG GTGGACGACC TCAGCGAGTT TGACCTGGAT GACATGAAAG CCGAGGGCCG GGAGCCTGCC CTGGTAGTGG AAACCAGCCC GAAGAACTAT CAGGCATGGG TCAAGGTGGC CGACGCCGCA GGCGGTGAAC TTCGGGGGCA GATTGCCCGG ACGCTGGCCA GCGAGTACGA CGCCGACCCG GCCAGCGCCG ACAGCCGCCA CTATGGCCGC TTGGCGGGCT TCACCAACCG CAAGGACAAG CACACCACCC GCGCCGGTTA TCAGCCGTGG GTGCTGCTGC GTGAATCCAA GGGCAAGACC GCCACCGCTG GCCCGGCGCT GGTGCAGCAG GCTGGCCAGC AGATCGAGCA GGCCCAGCGG CAGCAGGAGA AGGCCCGCAG GCTGGCCAGC CTCGAACTGC CCGAGCGGCA GCTTAGCCGC CACCGGCGCA CGGCGCTGGA CGAGTACCGC AGCGAGATGG CCGGGCTGGT CAAGCGCTTC GGTCATGACC TCAGCAAGTG CGACTTTATC GCCGCGCAGA AGCTGGCCAG CCGGGGCCGC AGTGCCGAGG AAATCGGCAA GGCCATGGCC GAGGCCAGCC CAGCGCTGGC AGAGCGCAAG CCCGGCCACG AAGCGGATTA CATCGAGCGC ACCGTCAGCA AGGTCATGGG TCTGCCCAGC GTCCAGCTTG CGCGGGCCGA GCTGGCACGG GCACCGGCAC CCCGCCAGCG AGGCATGGAC AGGGGCGGGC CAGATTTCAG CATGTAGTGC TTGCGTTGGT ACTCACGCCT GTTATACTAT GAGTACTCAC GCACAGAAGG GGGTTTTATG GAATACGAAA AAAGCGCTTC AGGGTCGGTC TACCTGATCA AAAGTGACAA GGGCTATTGG TTGCCCGGTG GCTTTGGTTA TACGTCAAAC AAGGCCGAGG CTGGCCGCTT TTCAGTCGCT GATATGGCCA GCCTTAACCT TGACGGCTGC ACCTTGTCCT TGTTCCGCGA AGACAAGCCT TTCGGCCCCG GCAAGTTTCT CGGTGACTGA TATGAAAGAC CAAAAGGACA AGCAGACCGG CGACCTGCTG GCCAGCCCTG ACGCTGTACG CCAAGCGCGA TATGCCGAGC GCATGAAGGC CAAAGGGATG CGTCAGCGCA AGTTCTGGCT GACCGACGAC GAATACGAGG CGCTGCGCGA GTGCCTGGAA GAACTCAGAG CGGCGCAGGG CGGGGGTAGT GACCCCGCCA GCGCCTAACC ACCAACTGCC TGCAAAGGAG GCAATCAATG GCTACCCATA AGCCTATCAA TATTCTGGAG GCGTTCGCAG CAGCGCCGCC ACCGCTGGAC TACGTTTTGC CCAACATGGT GGCCGGTACG GTCGGGGCGC TGGTGTCGCC CGGTGGTGCC GGTAAATCCA TGCTGGCCCT GCAACTGGCC GCACAGATTG CAGGCGGGCC GGATCTGCTG GAGGTGGGCG AACTGCCCAC CGGCCCGGTG ATCTACCTGC CCGCCGAAGA CCCGCCCACC GCCATTCATC ACCGCCTGCA CGCCCTTGGG GCGCACCTCA GCGCCGAGGA ACGGCAAGCC GTGGCTGACG GCCTGCTGAT CCAGCCGCTG ATCGGCAGCC TGCCCAACAT CATGGCCCCG GAGTGGTTCG ACGGCCTCAA GCGCGCCGCC GAGGGCCGCC GCCTGATGGT GCTGGACACG CTGCGCCGGT TCCACATCGA GGAAGAAAAC GCCAGCGGCC CCATGGCCCA GGTCATCGGT CGCATGGAGG CCATCGCCGC CGATACCGGG TGCTCTATCG TGTTCCTGCA CCATGCCAGC AAGGGCGCGG CCATGATGGG CGCAGGCGAC CAGCAGCAGG CCAGCCGGGG CAGCTCGGTA CTGGTCGATA ACATCCGCTG GCAGTCCTAC CTGTCGAGCA TGACCAGCGC CGAGGCCGAG GAATGGGGTG TGGACGACGA CCAGCGCCGG TTCTTCGTCC GCTTCGGTGT GAGCAAGGCC AACTATGGCG CACCGTTCGC TGATCGGTGG TTCAGGCGGC ATGACGGCGG GGTGCTCAAG CCCGCCGTGC TGGAGAGGCA GCGCAAGAGC AAGGGGGTGC CCCGTGGTGA AGCCTAAGAA CAAGCACAGC CTCAGCCACG TCCGGCACGA CCCGGCGCAC TGTCTGGCCC CCGGCCTGTT CCGTGCCCTC AAGCGGGGCG AGCGCAAGCG CAGCAAGCTG GACGTGACGT ATGACTACGG CGACGGCAAG CGGATCGAGT TCAGCGGCCC GGAGCCGCTG GGCGCTGATG ATCTGCGCAT CCTGCAAGGG CTGGTGGCCA TGGCTGGGCC TAATGGCCTA GTGCTTGGCC CGGAACCCAA GACCGAAGGC GGACGGCAGC TCCGGCTGTT CCTGGAACCC AAGTGGGAGG CCGTCACCGC TGAATGCCAT GTGGTCAAAG GTAGCTATCG GGCGCTGGCA AAGGAAATCG GGGCAGAGGT CGATAGTGGT GGGGCGCTCA AGCACATACA GGACTGCATC GAGCGCCTTT GGAAGGTATC CATCATCGCC CAGAATGGCC GCAAGCGGCA GGGGTTTCGG CTGCTGTCGG AGTACGCCAG CGACGAGGCG GACGGGCGCC TGTACGTGGC CCTGAACCCC TTGATCGCGC AGGCCGTCAT GGGTGGCGGC CAGCATGTGC GCATCAGCAT GGACGAGGTG CGGGCGCTGG ACAGCGAAAC CGCCCGCCTG CTGCACCAGC GGCTGTGTGG CTGGATCGAC CCCGGCAAAA CCGGCAAGGC TTCCATAGAT ACCTTGTGCG GCTATGTCTG GCCGTCAGAG GCCAGTGGTT CGACCATGCG CAAGCGCCGC AAGCGGGTGC GCGAGGCGTT GCCGGAGCTG GTCGCGCTGG GCTGGACGGT AACCGAGTTC GCGGCGGGCA AGTACGACAT CACCCGGCCC AAGGCGGCAG GCTGACCCCC CCCACTCTAT TGTAAACAAG ACATTTTTAT CTTTTATATT CAATGGCTTA TTTTCCTGCT AATTGGTAAT ACCATGAAAA ATACCATGCT CAGAAAAGGC TTAACAATAT TTTGAAAAAT TGCCTACTGA GCGCTGCCGC ACAGCTCCAT AGGCCGCTTT CCTGGCTTTG CTTCCAGATG TATGCTCTTC TGCTCCCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CAGCTTGCAA TTCGCGCTAA CTTACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCCAGGGT GGTTTTTCTT TTCACCAGTG AGACGGGCAA CAGCTGATTG CCCTTCACCG CCTGGCCCTG AGAGAGTTGC AGCAAGCGGT CCACGCTGGT TTGCCCCAGC AGGCGAAAAT CCTGTTTGAT GGTGGTTAAC GGCGGGATAT AACATGAGCT GTCTTCGGTA TCGTCGTATC CCACTACCGA GATATCCGCA CCAACGCGCA GCCCGGACTC GGTAATGGCG CGCATTGCGC CCAGCGCCAT CTGATCGTTG GCAACCAGCA TCGCAGTGGG AACGATGCCC TCATTCAGCA TTTGCATGGT TTGTTGAAAA CCGGACATGG CACTCCAGTC GCCTTCCCGT TCCGCTATCG GCTGAATTTG ATTGCGAGTG AGATATTTAT GCCAGCCAGC CAGACGCAGA CGCGCCGAGA CAGAACTTAA TGGGCCCGCT AACAGCGCGA TTTGCTGGTG ACCCAATGCG ACCAGATGCT CCACGCCCAG TCGCGTACCG TCTTCATGGG AGAAAATAAT ACTGTTGATG GGTGTCTGGT CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC AGCTTCCACA GCAATGGCAT CCTGGTCATC CAGCGGATAG TTAATGATCA GCCCACTGAC GCGTTGCGCG AGAAGATTGT GCACCGCCGC TTTACAGGCT TCGACGCCGC TTCGTTCTAC CATCGACACC ACCACGCTGG CACCCAGTTG ATCGGCGCGA GATTTAATCG CCGCGACAAT TTGCGACGGC GCGTGCAGGG CCAGACTGGA GGTGGCAACG CCAATCAGCA ACGACTGTTT GCCCGCCAGT TGTTGTGCCA CGCGGTTGGG AATGTAATTC AGCTCCGCCA TCGCCGCTTC CACTTTTTCC CGCGTTTTCG CAGAAACGTG GCTGGCCTGG TTCACCACGC GGGAAACGGT CTGATAAGAG ACACCGGCAT ACTCTGCGAC ATCGTATAAC GTTACTGGTT TCACATTCAC CACCCTGAAT TGACTCTCTT CCGGGCGCTA TCATGCCATA CCGCGAAAGG TTTTGCACCA TTCGATGGTG TCAACGTAAA TGCCGCTTCG CCTTCGCGCG CGAATTGCAA GCTGATCCGG GCTTATCGAC TGCACGGTGC ACCAATGCTT CTGGCGTCAG GCAGCCATCG GAAGCTGTGG TATGGCTGTG CAGGTCGTAA ATCACTGCAT AATTCGTGTC GCTCAAGGCG CACTCCCGTT CTGGATAATG TTTTTTGCGC CGACATCATA ACGGTTCTGG CAAATATTCT GAAATGAGCT GTTGACAATT AATCATCGGC TCGTATAATG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GAATTCGAGC TCGGTACCCG GGGATCCTCT AGAGTCGACC TGCAGGCATG CAAGCTTGGC TGTTTTGGCG GATGAGAGAA GA
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