pSET4S
$70.00
In stock
SKU
PL82250
100μg liquid plasmid(Instantly):$91
| name | pSET4S | another name | apSET4S |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Fragment type | ||
| Fragment species | Prokaryotic resistance | Spe | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pBR322 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTAGTTATC GGCATAATCG TTAAAACAGG CGTTATCGTA GCGTAAAAGC CCTTGAGCGT AGCGTGGCTT TGCAGCGAAG ATGTTGTCTG TTAGATTATG AAAGCCGATG ACTGAATGAA ATAATAAGCG CAGCGCCCTT CTATTTCGGT TGGAGGAGGC TCAAGGGAGT ATGAGGGAAT GAAATTCCCT CATGGGTTTG ATTTTAAAAA TTGCTTGCAA TTTTGCCGAG CGGTAGCGCT GGAAAATTTT TGAAAAAAAT TTGGAATTTG GAAAAAAATG GGGGGAAAGG AAGCGAATTT TGCTTCCGTA CTACGACCCC CCATTAAGTG CCGAGTGCCA ATTTTTGTGC CAAAAACGCT CTATCCCAAC TGGCTCAAGG GTTTAAGGGG TTTTTCAATC GCCAACGAAT CGCCAACGTT TTCGCCAACG TTTTTTATAA ATCTATATTT AAGTAGCTTT ATTGTTGTTT TTATGATTAC AAAGTGATAC ACTAACTTTA TAAAATTATT TGATTGGAGT TTTTTAAATG GTGATTTCAG AATCGAAAAA AAGAGTTATG ATTTCTCTGA CAAAAGAGCA AGATAAAAAA TTAACAGATA TGGCGAAACA AAAAGGTTTT TCAAAATCTG CGGTTGCGGC GTTAGCTATA GAAGAATATG CAAGAAAGGA ATCAGAACAA AAAAAATAAG CGAAAGCTCG CGTTTTTAGA AGGATACGAG TTTTCGCTAC TTGTTTTTGA TAAGGTAATT ATATCATGGC TATTAAAAAT ACTAAAGCTA GAAATTTTGG ATTTTTATTA TATCCTGACT CAATTCCTAA TGATTGGAAA GAAAAATTAG AGAGTTTGGG CGTATCTATG GCTGTCAGTC CTTTACACGA TATGGACGAA AAAAAAGATA AAGATACATG GAATAATAGT AATATTATAC AAAATGGAAA GCACTATAAA AAACCACACT ATCACGTTAT ATATATTGCA CGAAATCCTG TAACAATAGA AAGCGTTAGG AACAAGATTA AGCGAAAATT GGGGAATAGT TCAGTTGCTC ATGTTGAGAT ACTTGATTAT ATCAAAGGTT CATATGAATA TTTGACTCAT GAATCAAAGG ACGCTATTGC TAAGAATAAA CATATATACG ACAAAAAAGA TATTTTGAAC ATTAATGATT TTGATATTGA CCGCTATATA ACACTTGATG AAAGCCAAAA AAGAGAATTG AAGAATTTAC TTTTAGATAT AGTGGATGAC TATAATTTGG TAAATACAAA AGATTTAATG GCTTTTATTC GCCTTAGGGG AGCGGAGTTT GGAATTTTAA ATACGAATGA TGTAAAAGAT ATTGTTTCAA CAAACTCTAG CGCCTTTAGA TTATGGTTTG AGGGCAATTA TCAGTGTGGA TATAGAGCAA GTTATGCAAA GGTTCTTGAT GCTGAAACGG GGGAAATAAA ATGACAAACA AAGAAAAAGA GTTATTTGCT GAAAATGAGG AATTAAAAAA AGAAATTAAG GACTTAAAAG AGCGTATTGA AAGATACAGA GAAATGGAAG TTGAATTAAG TACAACAATA GATTTATTGA GAGGAGGGAT TATTGAATAA ATAAAAGCCC CCTGACGAAA GTCGAAGGGG GTTTTTATTT TGGTTTGATG TTGCGATTAA TAGATCTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTCGA GCTCGGTACC CGGGGATCCT CTAGAGTCGA CCTGCAGGCA TGCAAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTAGTGTTC GTGAATACAT GTTATAATAA CTATAACTAA TAACGTAACG TGACTGGCAA GAGATATTTT TAAAACAATG AATAGGTTTA CACTTACTTT AGTTTTATGG AAATGAAAGA TCATATCATA TATAATCTAG AATAAAATTA ACTAAAATAA TTATTATCTA GATAAAAAAT TTAGAAGCCA ATGAAATCTA TAAATAAACT AAATTAAGTT TATTTAATTA ACAACTATGG ATATAAAATA GGTACTAATC AAAATAGTGA GGAGGATATA TTTGAATACA TACGAACAAA TTAATAAAGT GAAAAAAATA CTTCGGAAAC ATTTAAAAAA TAACCTTATT GGTACTTACA TGTTTGGATC AGGAGTTGAG AGTGGACTAA AACCAAATAG TGATCTTGAC TTTTTAGTCG TCGTATCTGA ACCATTGACA GATCAAAGTA AAGAAATACT TATACAAAAA ATTAGACCTA TTTCAAAAAA AATAGGAGAT AAAAGCAACT TACGATATAT TGAATTAACA ATTATTATTC AGCAAGAAAT GGTACCGTGG AATCATCCTC CCAAACAAGA ATTTATTTAT GGAGAATGGT TACAAGAGCT TTATGAACAA GGATACATTC CTCAGAAGGA ATTAAATTCA GATTTAACCA TAATGCTTTA CCAAGCAAAA CGAAAAAATA AAAGAATATA CGGAAATTAT GACTTAGAGG AATTACTACC TGATATTCCA TTTTCTGATG TGAGAAGAGC CATTATGGAT TCGTCAGAGG AATTAATAGA TAATTATCAG GATGATGAAA CCAACTCTAT ATTAACTTTA TGCCGTATGA TTTTAACTAT GGACACGGGT AAAATCATAC CAAAAGATAT TGCGGGAAAT GCAGTGGCTG AATCTTCTCC ATTAGAACAT AGGGAGAGAA TTTTGTTAGC AGTTCGTAGT TATCTTGGAG AGAATATTGA ATGGACTAAT GAAAATGTAA ATTTAACTAT AAACTATTTA AATAACAGAT TAAAAAAATT ATAAAAAAAT TGAAAAAATG GTGGAAACAC TTTTTTCAAT TTTTTTGTTT TATTATTTAA TATTTGGGAA ATATTCATTC TAATTGGTAA TCAGATTTTA GAAAAC
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
