pAO815
$99.00
In stock
SKU
PL82187
100μg liquid plasmid(Instantly):$120
| name | pAO815 | another name | apAO815 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | HIS4 | Fluorescent labeling | |
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGGCA TACCGTTTGT CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAACG AGGAATTCGC CTTAGACATG ACTGTTCCTC AGTTCAAGTT GGGCACTTAC GAGAAGACCG GTCTTGCTAG ATTCTAATCA AGAGGATGTC AGAATGCCAT TTGCCTGAGA GATGCAGGCT TCATTTTTGA TACTTTTTTA TTTGTAACCT ATATAGTATA GGATTTTTTT TGTCATTTTG TTTCTTCTCG TACGAGCTTG CTCCTGATCA GCCTATCTCG CAGCTGATGA ATATCTTGTG GTAGGGGTTT GGGAAAATCA TTCGAGTTTG ATGTTTTTCT TGGTATTTCC CACTCCTCTT CAGAGTACAG AAGATTAAGT GAGACGTTCG TTTGTGCGGA TCCAATGCGG TAGTTTATCA CAGTTAAATT GCTAACGCAG TCAGGCACCG TGTATGAAAT CTAACAATGC GCTCATCGTC ATCCTCGGCA CCGTCACCCT GGATGCTGTA GGCATAGGCT TGGTTATGCC GGTACTGCCG GGCCTCTTGC GGGATATCGT CCATTCCGAC AGCATCGCCA GTCACTATGG CGTGCTGCTA GCGCTATATG CGTTGATGCA ATTTCTATGC GCACCCGTTC TCGGAGCACT GTCCGACCGC TTTGGCCGCC GCCCAGTCCT GCTCGCTTCG CTACTTGGAG CCACTATCGA CTACGCGATC ATGGCGACCA CACCCGTCCT GTGGATCTAT CGAATCTAAA TGTAAGTTAA AATCTCTAAA TAATTAAATA AGTCCCAGTT TCTCCATACG AACCTTAACA GCATTGCGGT GAGCATCTAG ACCTTCAACA GCAGCCAGAT CCATCACTGC TTGGCCAATA TGTTTCAGTC CCTCAGGAGT TACGTCTTGT GAAGTGATGA ACTTCTGGAA GGTTGCAGTG TTAACTCCGC TGTATTGACG GGCATATCCG TACGTTGGCA AAGTGTGGTT GGTACCGGAG GAGTAATCTC CACAACTCTC TGGAGAGTAG GCACCAACAA ACACAGATCC AGCGTGTTGT ACTTGATCAA CATAAGAAGA AGCATTCTCG ATTTGCAGGA TCAAGTGTTC AGGAGCGTAC TGATTGGACA TTTCCAAAGC CTGCTCGTAG GTTGCAACCG ATAGGGTTGT AGAGTGTGCA ATACACTTGC GTACAATTTC AACCCTTGGC AACTGCACAG CTTGGTTGTG AACAGCATCT TCAATTCTGG CAAGCTCCTT GTCTGTCATA TCGACAGCCA ACAGAATCAC CTGGGAATCA ATACCATGTT CAGCTTGAGA CAGAAGGTCT GAGGCAACGA AATCTGGATC AGCGTATTTA TCAGCAATAA CTAGAACTTC AGAAGGCCCA GCAGGCATGT CAATACTACA CAGGGCTGAT GTGTCATTTT GAACCATCAT CTTGGCAGCA GTAACGAACT GGTTTCCTGG ACCAAATATT TTGTCACACT TAGGAACAGT TTCTGTTCCG TAAGCCATAG CAGCTACTGC CTGGGCGCCT CCTGCTAGCA CGATACACTT AGCACCAACC TTGTGGGCAA CGTAGATGAC TTCTGGGGTA AGGGTACCAT CCTTCTTAGG TGGAGATGCA AAAACAATTT CTTTGCAACC AGCAACTTTG GCAGGAACAC CCAGCATCAG GGAAGTGGAA GGCAGAATTG CGGTTCCACC AGGAATATAG AGGCCAACTT TCTCAATAGG TCTTGCAAAA CGAGAGCAGA CTACACCAGG GCAAGTCTCA ACTTGCAACG TCTCCGTTAG TTGAGCTTCA TGGAATTTCC TGACGTTATC TATAGAGAGA TCAATGGCTC TCTTAACGTT ATCTGGCAAT TGCATAAGTT CCTCTGGGAA AGGAGCTTCT AACACAGGTG TCTTCAAAGC GACTCCATCA AACTTGGCAG TTAGTTCTAA AAGGGCTTTG TCACCATTTT GACGAACATT GTCGACAATT GGTTTGACTA ATTCCATAAT CTGTTCCGTT TTCTGGATAG GACGACGAAG GGCATCTTCA ATTTCTTGTG AGGAGGCCTT AGAAACGTCA ATTTTGCACA ATTCAATACG ACCTTCAGAA GGGACTTCTT TAGGTTTGGA TTCTTCTTTA GGTTGTTCCT TGGTGTATCC TGGCTTGGCA TCTCCTTTCC TTCTAGTGAC CTTTAGGGAC TTCATATCCA GGTTTCTCTC CACCTCGTCC AACGTCACAC CGTACTTGGC ACATCTAACT AATGCAAAAT AAAATAAGTC AGCACATTCC CAGGCTATAT CTTCCTTGGA TTTAGCTTCT GCAAGTTCAT CAGCTTCCTC CCTAATTTTA GCGTTCAACA AAACTTCGTC GTCAAATAAC CGTTTGGTAT AAGAACCTTC TGGAGCATTG CTCTTACGAT CCCACAAGGT GGCTTCCATG GCTCTAAGAC CCTTTGATTG GCCAAAACAG GAAGTGCGTT CCAAGTGACA GAAACCAACA CCTGTTTGTT CAACCACAAA TTTCAAGCAG TCTCCATCAC AATCCAATTC GATACCCAGC AACTTTTGAG TTGCTCCAGA TGTAGCACCT TTATACCACA AACCGTGACG ACGAGATTGG TAGACTCCAG TTTGTGTCCT TATAGCCTCC GGAATAGACT TTTTGGACGA GTACACCAGG CCCAACGAGT AATTAGAAGA GTCAGCCACC AAAGTAGTGA ATAGACCATC GGGGCGGTCA GTAGTCAAAG ACGCCAACAA AATTTCACTG ACAGGGAACT TTTTGACATC TTCAGAAAGT TCGTATTCAG TAGTCAATTG CCGAGCATCA ATAATGGGGA TTATACCAGA AGCAACAGTG GAAGTCACAT CTACCAACTT TGCGGTCTCA GAAAAAGCAT AAACAGTTCT ACTACCGCCA TTAGTGAAAC TTTTCAAATC GCCCAGTGGA GAAGAAAAAG GCACAGCGAT ACTAGCATTA GCGGGCAAGG ATGCAACTTT ATCAACCAGG GTCCTATAGA TAACCCTAGC GCCTGGGATC ATCCTTTGGA CAACTCTTTC TGCCAAATCT AGGTCCAAAA TCACTTCATT GATACCATTA TTGTACAACT TGAGCAAGTT GTCGATCAGC TCCTCAAATT GGTCCTCTGT AACGGATGAC TCAACTTGCA CATTAACTTG AAGCTCAGTC GATTGAGTGA ACTTGATCAG GTTGTGCAGC TGGTCAGCAG CATAGGGAAA CACGGCTTTT CCTACCAAAC TCAAGGAATT ATCAAACTCT GCAACACTTG CGTATGCAGG TAGCAAGGGA AATGTCATAC TTGAAGTCGG ACAGTGAGTG TAGTCTTGAG AAATTCTGAA GCCGTATTTT TATTATCAGT GAGTCAGTCA TCAGGAGATC CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGCGCCA CAGGTGCGGT TGCTGGCGCC TATATCGCCG ACATCACCGA TGGGGAAGAT CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCATGCAC CATTCCTTGC GGCGGCGGTG CTCAACGGCC TCAACCTACT ACTGGGCTGC TTCCTAATGC AGGAGTCGCA TAAGGGAGAG CGTCGAGTAT CTATGATTGG AAGTATGGGA ATGGTGATAC CCGCATTCTT CAGTGTCTTG AGGTCTCCTA TCAGATTATG CCCAACTAAA GCAACCGGAG GAGGAGATTT CATGGTAAAT TTCTCTGACT TTTGGTCATC AGTAGACTCG AACTGTGAGA CTATCTCGGT TATGACAGCA GAAATGTCCT TCTTGGAGAC AGTAAATGAA GTCCCACCAA TAAAGAAATC CTTGTTATCA GGAACAAACT TCTTGTTTCG AACTTTTTCG GTGCCTTGAA CTATAAAATG TAGAGTGGAT ATGTCGGGTA GGAATGGAGC GGGCAAATGC TTACCTTCTG GACCTTCAAG AGGTATGTAG GGTTTGTAGA TACTGATGCC AACTTCAGTG ACAACGTTGC TATTTCGTTC AAACCATTCC GAATCCAGAG AAATCAAAGT TGTTTGTCTA CTATTGATCC AAGCCAGTGC GGTCTTGAAA CTGACAATAG TGTGCTCGTG TTTTGAGGTC ATCTTTGTAT GAATAAATCT AGTCTTTGAT CTAAATAATC TTGACGAGCC AAGGCGATAA ATACCCAAAT CTAAAACTCT TTTAAAACGT TAAAAGGACA AGTATGTCTG CCTGTATTAA ACCCCAAATC AGCTCGTAGT CTGATCCTCA TCAACTTGAG GGGCACTATC TTGTTTTAGA GAAATTTGCG GAGATGCGAT ATCGAGAAAA AGGTACGCTG ATTTTAAACG TGAAATTTAT CTCAAGATCT GCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC ACGTAGCGAT AGCGGAGTGT ATACTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTGCAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAACACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTT CAAGAATTAA TTCTCATGTT TGACAGCTTA TCATCGATAA GCTGACTCAT GTTGGTATTG TGAAATAGAC GCAGATCGGG AACACTGAAA AATAACAGTT ATTATTCG
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