NM_001127500.3;AUTS9; c-Met; DA11; DFNB97; HGFR; RCCP2
100μg liquid plasmid(Instantly):$149
| name | pRetroX-3×FLAG-MET(human)-mCherry-Puro | another name | apRetroX-3×FLAG-MET(human)-mCherry-Puro |
| Insert Size (bp) | 4224bp | Plasmid host | Mammary cell,retrovirus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Red |
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC CGGGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGCCT CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG GAACTGACGA GTTCGGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCGGGG GCCGTTTTTG TGGCCCGACC TGAGTCCAAA AATCCCGATC GTTTTGGACT CTTTGGTGCA CCCCCCTTAG AGGAGGGATA TGTGGTTCTG GTAGGAGACG AGAACCTAAA ACAGTTCCCG CCTCCGTCTG AATTTTTGCT TTCGGTTTGG GACCGAAGCC GCGCCGCGCG TCTTGTCTGC TGCAGCATCG TTCTGTGTTG TCTCTGTCTG ACTGTGTTTC TGTATTTGTC TGAAAATACG CGTGTCGAGC TTTTGGAGTA CGTCGTCTTT AGGTTGGGGG GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGCC GTCGTGAAAA CTACCCCTAA AAGCCAAAAA AATCGAATTC CCAAACTTAA GCTTGGTACC GAGCTCGGAT CTCACGTGGG CCCGCGGCCG CAGATCTATC GATGGATCCG CCACCATGGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGAAGGCC CCCGCTGTGC TTGCACCTGG CATCCTCGTG CTCCTGTTTA CCTTGGTGCA GAGGAGCAAT GGGGAGTGTA AAGAGGCACT AGCAAAGTCC GAGATGAATG TGAATATGAA GTATCAGCTT CCCAACTTCA CCGCGGAAAC ACCCATCCAG AATGTCATTC TACATGAGCA TCACATTTTC CTTGGTGCCA CTAACTACAT TTATGTTTTA AATGAGGAAG ACCTTCAGAA GGTTGCTGAG TACAAGACTG GGCCTGTGCT GGAACACCCA GATTGTTTCC CATGTCAGGA CTGCAGCAGC AAAGCCAATT TATCAGGAGG TGTTTGGAAA GATAACATCA ACATGGCTCT AGTTGTCGAC ACCTACTATG ATGATCAACT CATTAGCTGT GGCAGCGTCA ACAGAGGGAC CTGCCAGCGA CATGTCTTTC CCCACAATCA TACTGCTGAC ATACAGTCGG AGGTTCACTG CATATTCTCC CCACAGATAG AAGAGCCCAG CCAGTGTCCT GACTGTGTGG TGAGCGCCCT GGGAGCCAAA GTCCTTTCAT CTGTAAAGGA CCGGTTCATC AACTTCTTTG TAGGCAATAC CATAAATTCT TCTTATTTCC CAGATCATCC ATTGCATTCG ATATCAGTGA GAAGGCTAAA GGAAACGAAA GATGGTTTTA TGTTTTTGAC GGACCAGTCC TACATTGATG TTTTACCTGA GTTCAGAGAT TCTTACCCCA TTAAGTATGT CCATGCCTTT GAAAGCAACA ATTTTATTTA CTTCTTGACG GTCCAAAGGG AAACTCTAGA TGCTCAGACT TTTCACACAA GAATAATCAG GTTCTGTTCC ATAAACTCTG GATTGCATTC CTACATGGAA ATGCCTCTGG AGTGTATTCT CACAGAAAAG AGAAAAAAGA GATCCACAAA GAAGGAAGTG TTTAATATAC TTCAGGCTGC GTATGTCAGC AAGCCTGGGG CCCAGCTTGC TAGACAAATA GGAGCCAGCC TGAATGATGA CATTCTTTTC GGGGTGTTCG CACAAAGCAA GCCAGATTCT GCCGAACCAA TGGATCGATC TGCCATGTGT GCATTCCCTA TCAAATATGT CAACGACTTC TTCAACAAGA TCGTCAACAA AAACAATGTG AGATGTCTCC AGCATTTTTA CGGACCCAAT CATGAGCACT GCTTTAATAG GACACTTCTG AGAAATTCAT CAGGCTGTGA AGCGCGCCGT GATGAATATC GAACAGAGTT TACCACAGCT TTGCAGCGCG TTGACTTATT CATGGGTCAA TTCAGCGAAG TCCTCTTAAC ATCTATATCC ACCTTCATTA AAGGAGACCT CACCATAGCT AATCTTGGGA CATCAGAGGG TCGCTTCATG CAGGTTGTGG TTTCTCGATC AGGACCATCA ACCCCTCATG TGAATTTTCT CCTGGACTCC CATCCAGTGT CTCCAGAAGT GATTGTGGAG CATACATTAA ACCAAAATGG CTACACACTG GTTATCACTG GGAAGAAGAT CACGAAGATC CCATTGAATG GCTTGGGCTG CAGACATTTC CAGTCCTGCA GTCAATGCCT CTCTGCCCCA CCCTTTGTTC AGTGTGGCTG GTGCCACGAC AAATGTGTGC GATCGGAGGA ATGCCTGAGC GGGACATGGA CTCAACAGAT CTGTCTGCCT GCAATCTACA AGGTTTTCCC AAATAGTGCA CCCCTTGAAG GAGGGACAAG GCTGACCATA TGTGGCTGGG ACTTTGGATT TCGGAGGAAT AATAAATTTG ATTTAAAGAA AACTAGAGTT CTCCTTGGAA ATGAGAGCTG CACCTTGACT TTAAGTGAGA GCACGATGAA TACATTGAAA TGCACAGTTG GTCCTGCCAT GAATAAGCAT TTCAATATGT CCATAATTAT TTCAAATGGC CACGGGACAA CACAATACAG TACATTCTCC TATGTGGATC CTGTAATAAC AAGTATTTCG CCGAAATACG GTCCTATGGC TGGTGGCACT TTACTTACTT TAACTGGAAA TTACCTAAAC AGTGGGAATT CTAGACACAT TTCAATTGGT GGAAAAACAT GTACTTTAAA AAGTGTGTCA AACAGTATTC TTGAATGTTA TACCCCAGCC CAAACCATTT CAACTGAGTT TGCTGTTAAA TTGAAAATTG ACTTAGCCAA CCGAGAGACA AGCATCTTCA GTTACCGTGA AGATCCCATT GTCTATGAAA TTCATCCAAC CAAATCTTTT ATTAGTACTT GGTGGAAAGA ACCTCTCAAC ATTGTCAGTT TTCTATTTTG CTTTGCCAGT GGTGGGAGCA CAATAACAGG TGTTGGGAAA AACCTGAATT CAGTTAGTGT CCCGAGAATG GTCATAAATG TGCATGAAGC AGGAAGGAAC TTTACAGTGG CATGTCAACA TCGCTCTAAT TCAGAGATAA TCTGTTGTAC CACTCCTTCC CTGCAACAGC TGAATCTGCA ACTCCCCCTG AAAACCAAAG CCTTTTTCAT GTTAGATGGG ATCCTTTCCA AATACTTTGA TCTCATTTAT GTACATAATC CTGTGTTTAA GCCTTTTGAA AAGCCAGTGA TGATCTCAAT GGGCAATGAA AATGTACTGG AAATTAAGGG AAATGATATT GACCCTGAAG CAGTTAAAGG TGAAGTGTTA AAAGTTGGAA ATAAGAGCTG TGAGAATATA CACTTACATT CTGAAGCCGT TTTATGCACG GTCCCCAATG ACCTGCTGAA ATTGAACAGC GAGCTAAATA TAGAGTGGAA GCAAGCAATT TCTTCAACCG TCCTTGGAAA AGTAATAGTT CAACCAGATC AGAATTTCAC AGGATTGATT GCTGGTGTTG TCTCAATATC AACAGCACTG TTATTACTAC TTGGGTTTTT CCTGTGGCTG AAAAAGAGAA AGCAAATTAA AGATCTGGGC AGTGAATTAG TTCGCTACGA TGCAAGAGTA CACACTCCTC ATTTGGATAG GCTTGTAAGT GCCCGAAGTG TAAGCCCAAC TACAGAAATG GTTTCAAATG AATCTGTAGA CTACCGAGCT ACTTTTCCAG AAGATCAGTT TCCTAATTCA TCTCAGAACG GTTCATGCCG ACAAGTGCAG TATCCTCTGA CAGACATGTC CCCCATCCTA ACTAGTGGGG ACTCTGATAT ATCCAGTCCA TTACTGCAAA ATACTGTCCA CATTGACCTC AGTGCTCTAA ATCCAGAGCT GGTCCAGGCA GTGCAGCATG TAGTGATTGG GCCCAGTAGC CTGATTGTGC ATTTCAATGA AGTCATAGGA AGAGGGCATT TTGGTTGTGT ATATCATGGG ACTTTGTTGG ACAATGATGG CAAGAAAATT CACTGTGCTG TGAAATCCTT GAACAGAATC ACTGACATAG GAGAAGTTTC CCAATTTCTG ACCGAGGGAA TCATCATGAA AGATTTTAGT CATCCCAATG TCCTCTCGCT CCTGGGAATC TGCCTGCGAA GTGAAGGGTC TCCGCTGGTG GTCCTACCAT ACATGAAACA TGGAGATCTT CGAAATTTCA TTCGAAATGA GACTCATAAT CCAACTGTAA AAGATCTTAT TGGCTTTGGT CTTCAAGTAG CCAAAGGCAT GAAATATCTT GCAAGCAAAA AGTTTGTCCA CAGAGACTTG GCTGCAAGAA ACTGTATGCT GGATGAAAAA TTCACAGTCA AGGTTGCTGA TTTTGGTCTT GCCAGAGACA TGTATGATAA AGAATACTAT AGTGTACACA ACAAAACAGG TGCAAAGCTG CCAGTGAAGT GGATGGCTTT GGAAAGTCTG CAAACTCAAA AGTTTACCAC CAAGTCAGAT GTGTGGTCCT TTGGCGTGCT CCTCTGGGAG CTGATGACAA GAGGAGCCCC ACCTTATCCT GACGTAAACA CCTTTGATAT AACTGTTTAC TTGTTGCAAG GGAGAAGACT CCTACAACCC GAATACTGCC CAGACCCCTT ATATGAAGTA ATGCTAAAAT GCTGGCACCC TAAAGCCGAA ATGCGCCCAT CCTTTTCTGA ACTGGTGTCC CGGATATCAG CGATCTTCTC TACTTTCATT GGGGAGCACT ATGTCCATGT GAACGCTACT TATGTGAACG TAAAATGTGT CGCTCCGTAT CCTTCTCTGT TGTCATCAGA AGATAACGCT GATGATGAGG TGGACACACG ACCAGCCTCC TTCTGGGAGA CATCATAGTC GAGACTAGTT CTAGAGCGGC CGCGGATCCC GCCCCTCTCC CTCCCCCCCC CCTAACGTTA CTGGCCGAAG CCGCTTGGAA TAAGGCCGGT GTGCGTTTGT CTATATGTTA TTTTCCACCA TATTGCCGTC TTTTGGCAAT GTGAGGGCCC GGAAACCTGG CCCTGTCTTC TTGACGAGCA TTCCTAGGGG TCTTTCCCCT CTCGCCAAAG GAATGCAAGG TCTGTTGAAT GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT TCTTGAAGAC AAACAACGTC TGTAGCGACC CTTTGCAGGC AGCGGAACCC CCCACCTGGC GACAGGTGCC TCTGCGGCCA AAAGCCACGT GTATAAGATA CACCTGCAAA GGCGGCACAA CCCCAGTGCC ACGTTGTGAG TTGGATAGTT GTGGAAAGAG TCAAATGGCT CACCTCAAGC GTATTCAACA AGGGGCTGAA GGATGCCCAG AAGGTACCCC ATTGTATGGG ATCTGATCTG GGGCCTCGGT GCACATGCTT TACATGTGTT TAGTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGT GAGCAAGGGC GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC ATGGGCTGGG AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT CAAGACCACC TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC ATCACCTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC CGAGGGCCGC CACTCCACCG GCGGCATGGA CGAGCTGTAC AAGTGAACAG GGACAGCAGA GATCCACTTT GGCCGCGGCT CGAGGGGGTT GGGGTTGCGC CTTTTCCAAG GCAGCCCTGG GTTTGCGCAG GGACGCGGCT GCTCTGGGCG TGGTTCCGGG AAACGCAGCG GCGCCGACCC TGGGTCTCGC ACATTCTTCA CGTCCGTTCG CAGCGTCACC CGGATCTTCG CCGCTACCCT TGTGGGCCCC CCGGCGACGC TTCCTGCTCC GCCCCTAAGT CGGGAAGGTT CCTTGCGGTT CGCGGCGTGC CGGACGTGAC AAACGGAAGC CGCACGTCTC ACTAGTACCC TCGCAGACGG ACAGCGCCAG GGAGCAATGG CAGCGCGCCG ACCGCGATGG GCTGTGGCCA ATAGCGGCTG CTCAGCAGGG CGCGCCGAGA GCAGCGGCCG GGAAGGGGCG GTGCGGGAGG CGGGGTGTGG GGCGGTAGTG TGGGCCCTGT TCCTGCCCGC GCGGTGTTCC GCATTCTGCA AGCCTCCGGA GCGCACGTCG GCAGTCGGCT CCCTCGTTGA CCGAATCACC GACCTCTCTC CCCAGGGGGA TCCACCGGAG CTTACCATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGACGCC CGCCCCACGA CCCGCAGCGC CCGACCGAAA GGAGCGCACG ACCCCATGCA TCGGTACCTT TAAGACCAAT GACTTACAAG GCAGTGTACA AGGCCAAGTC CGTGCCCCGC AAGATGCCCG ACTGGCACTT CATCCAGCAC AAGCTGACCC GCGAGGACCG CAGCGACGCC AAGAACCAGA AGTGGCACCT GACCGAGCAC GCCATCGCCT CCGGCTCCGC CTTGCCCTGA GCGGCTTCGA GGATAAAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA GAAGTTCAGA TCAAGGTCAG GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG GGGCGCCAGT CCTCCGATTG ACTGAGTCGC CCGGGTACCC GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC TTTCATTGAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG CCAAGCTTAT G
