pBeloBAC11
$157.00
In stock
SKU
PL82159
提取需要加诱导剂
100μg liquid plasmid(Instantly):$236
| name | pBeloBAC11 | another name | apBeloBAC11 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Artificial chromosomes | Fragment type | cDNA |
| Fragment species | Empty vector | Prokaryotic resistance | Chl |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | Ori2 | |
| Competence | EPI400 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Extremely low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCGGCCGCAA GGGGTTCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG GCGAATTCGA GCTCGGTACC CGGGGATCCT CTAGAGTCGA CCTGCAGGCA TGCAAGCTTG AGTATTCTAT AGTGTCACCT AAATAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGAACCCCTT GCGGCCGCCC GGGCCGTCGA CCAATTCTCA TGTTTGACAG CTTATCATCG AATTTCTGCC ATTCATCCGC TTATTATCAC TTATTCAGGC GTAGCAACCA GGCGTTTAAG GGCACCAATA ACTGCCTTAA AAAAATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCGATCAAC GTCTCATTTT CGCCAAAAGT TGGCCCAGGG CTTCCCGGTA TCAACAGGGA CACCAGGATT TATTTATTCT GCGAAGTGAT CTTCCGTCAC AGGTATTTAT TCGCGATAAG CTCATGGAGC GGCGTAACCG TCGCACAGGA AGGACAGAGA AAGCGCGGAT CTGGGAAGTG ACGGACAGAA CGGTCAGGAC CTGGATTGGG GAGGCGGTTG CCGCCGCTGC TGCTGACGGT GTGACGTTCT CTGTTCCGGT CACACCACAT ACGTTCCGCC ATTCCTATGC GATGCACATG CTGTATGCCG GTATACCGCT GAAAGTTCTG CAAAGCCTGA TGGGACATAA GTCCATCAGT TCAACGGAAG TCTACACGAA GGTTTTTGCG CTGGATGTGG CTGCCCGGCA CCGGGTGCAG TTTGCGATGC CGGAGTCTGA TGCGGTTGCG ATGCTGAAAC AATTATCCTG AGAATAAATG CCTTGGCCTT TATATGGAAA TGTGGAACTG AGTGGATATG CTGTTTTTGT CTGTTAAACA GAGAAGCTGG CTGTTATCCA CTGAGAAGCG AACGAAACAG TCGGGAAAAT CTCCCATTAT CGTAGAGATC CGCATTATTA ATCTCAGGAG CCTGTGTAGC GTTTATAGGA AGTAGTGTTC TGTCATGATG CCTGCAAGCG GTAACGAAAA CGATTTGAAT ATGCCTTCAG GAACAATAGA AATCTTCGTG CGGTGTTACG TTGAAGTGGA GCGGATTATG TCAGCAATGG ACAGAACAAC CTAATGAACA CAGAACCATG ATGTGGTCTG TCCTTTTACA GCCAGTAGTG CTCGCCGCAG TCGAGCGACA GGGCGAAGCC CTCGAGTGAG CGAGGAAGCA CCAGGGAACA GCACTTATAT ATTCTGCTTA CACACGATGC CTGAAAAAAC TTCCCTTGGG GTTATCCACT TATCCACGGG GATATTTTTA TAATTATTTT TTTTATAGTT TTTAGATCTT CTTTTTTAGA GCGCCTTGTA GGCCTTTATC CATGCTGGTT CTAGAGAAGG TGTTGTGACA AATTGCCCTT TCAGTGTGAC AAATCACCCT CAAATGACAG TCCTGTCTGT GACAAATTGC CCTTAACCCT GTGACAAATT GCCCTCAGAA GAAGCTGTTT TTTCACAAAG TTATCCCTGC TTATTGACTC TTTTTTATTT AGTGTGACAA TCTAAAAACT TGTCACACTT CACATGGATC TGTCATGGCG GAAACAGCGG TTATCAATCA CAAGAAACGT AAAAATAGCC CGCGAATCGT CCAGTCAAAC GACCTCACTG AGGCGGCATA TAGTCTCTCC CGGGATCAAA AACGTATGCT GTATCTGTTC GTTGACCAGA TCAGAAAATC TGATGGCACC CTACAGGAAC ATGACGGTAT CTGCGAGATC CATGTTGCTA AATATGCTGA AATATTCGGA TTGACCTCTG CGGAAGCCAG TAAGGATATA CGGCAGGCAT TGAAGAGTTT CGCGGGGAAG GAAGTGGTTT TTTATCGCCC TGAAGAGGAT GCCGGCGATG AAAAAGGCTA TGAATCTTTT CCTTGGTTTA TCAAACGTGC GCACAGTCCA TCCAGAGGGC TTTACAGTGT ACATATCAAC CCATATCTCA TTCCCTTCTT TATCGGGTTA CAGAACCGGT TTACGCAGTT TCGGCTTAGT GAAACAAAAG AAATCACCAA TCCGTATGCC ATGCGTTTAT ACGAATCCCT GTGTCAGTAT CGTAAGCCGG ATGGCTCAGG CATCGTCTCT CTGAAAATCG ACTGGATCAT AGAGCGTTAC CAGCTGCCTC AAAGTTACCA GCGTATGCCT GACTTCCGCC GCCGCTTCCT GCAGGTCTGT GTTAATGAGA TCAACAGCAG AACTCCAATG CGCCTCTCAT ACATTGAGAA AAAGAAAGGC CGCCAGACGA CTCATATCGT ATTTTCCTTC CGCGATATCA CTTCCATGAC GACAGGATAG TCTGAGGGTT ATCTGTCACA GATTTGAGGG TGGTTCGTCA CATTTGTTCT GACCTACTGA GGGTAATTTG TCACAGTTTT GCTGTTTCCT TCAGCCTGCA TGGATTTTCT CATACTTTTT GAACTGTAAT TTTTAAGGAA GCCAAATTTG AGGGCAGTTT GTCACAGTTG ATTTCCTTCT CTTTCCCTTC GTCATGTGAC CTGATATCGG GGGTTAGTTC GTCATCATTG ATGAGGGTTG ATTATCACAG TTTATTACTC TGAATTGGCT ATCCGCGTGT GTACCTCTAC CTGGAGTTTT TCCCACGGTG GATATTTCTT CTTGCGCTGA GCGTAAGAGC TATCTGACAG AACAGTTCTT CTTTGCTTCC TCGCCAGTTC GCTCGCTATG CTCGGTTACA CGGCTGCGGC GAGCGCTAGT GATAATAAGT GACTGAGGTA TGTGCTCTTC TTATCTCCTT TTGTAGTGTT GCTCTTATTT TAAACAACTT TGCGGTTTTT TGATGACTTT GCGATTTTGT TGTTGCTTTG CAGTAAATTG CAAGATTTAA TAAAAAAACG CAAAGCAATG ATTAAAGGAT GTTCAGAATG AAACTCATGG AAACACTTAA CCAGTGCATA AACGCTGGTC ATGAAATGAC GAAGGCTATC GCCATTGCAC AGTTTAATGA TGACAGCCCG GAAGCGAGGA AAATAACCCG GCGCTGGAGA ATAGGTGAAG CAGCGGATTT AGTTGGGGTT TCTTCTCAGG CTATCAGAGA TGCCGAGAAA GCAGGGCGAC TACCGCACCC GGATATGGAA ATTCGAGGAC GGGTTGAGCA ACGTGTTGGT TATACAATTG AACAAATTAA TCATATGCGT GATGTGTTTG GTACGCGATT GCGACGTGCT GAAGACGTAT TTCCACCGGT GATCGGGGTT GCTGCCCATA AAGGTGGCGT TTACAAAACC TCAGTTTCTG TTCATCTTGC TCAGGATCTG GCTCTGAAGG GGCTACGTGT TTTGCTCGTG GAAGGTAACG ACCCCCAGGG AACAGCCTCA ATGTATCACG GATGGGTACC AGATCTTCAT ATTCATGCAG AAGACACTCT CCTGCCTTTC TATCTTGGGG AAAAGGACGA TGTCACTTAT GCAATAAAGC CCACTTGCTG GCCGGGGCTT GACATTATTC CTTCCTGTCT GGCTCTGCAC CGTATTGAAA CTGAGTTAAT GGGCAAATTT GATGAAGGTA AACTGCCCAC CGATCCACAC CTGATGCTCC GACTGGCCAT TGAAACTGTT GCTCATGACT ATGATGTCAT AGTTATTGAC AGCGCGCCTA ACCTGGGTAT CGGCACGATT AATGTCGTAT GTGCTGCTGA TGTGCTGATT GTTCCCACGC CTGCTGAGTT GTTTGACTAC ACCTCCGCAC TGCAGTTTTT CGATATGCTT CGTGATCTGC TCAAGAACGT TGATCTTAAA GGGTTCGAGC CTGATGTACG TATTTTGCTT ACCAAATACA GCAATAGTAA TGGCTCTCAG TCCCCGTGGA TGGAGGAGCA AATTCGGGAT GCCTGGGGAA GCATGGTTCT AAAAAATGTT GTACGTGAAA CGGATGAAGT TGGTAAAGGT CAGATCCGGA TGAGAACTGT TTTTGAACAG GCCATTGATC AACGCTCTTC AACTGGTGCC TGGAGAAATG CTCTTTCTAT TTGGGAACCT GTCTGCAATG AAATTTTCGA TCGTCTGATT AAACCACGCT GGGAGATTAG ATAATGAAGC GTGCGCCTGT TATTCCAAAA CATACGCTCA ATACTCAACC GGTTGAAGAT ACTTCGTTAT CGACACCAGC TGCCCCGATG GTGGATTCGT TAATTGCGCG CGTAGGAGTA ATGGCTCGCG GTAATGCCAT TACTTTGCCT GTATGTGGTC GGGATGTGAA GTTTACTCTT GAAGTGCTCC GGGGTGATAG TGTTGAGAAG ACCTCTCGGG TATGGTCAGG TAATGAACGT GACCAGGAGC TGCTTACTGA GGACGCACTG GATGATCTCA TCCCTTCTTT TCTACTGACT GGTCAACAGA CACCGGCGTT CGGTCGAAGA GTATCTGGTG TCATAGAAAT TGCCGATGGG AGTCGCCGTC GTAAAGCTGC TGCACTTACC GAAAGTGATT ATCGTGTTCT GGTTGGCGAG CTGGATGATG AGCAGATGGC TGCATTATCC AGATTGGGTA ACGATTATCG CCCAACAAGT GCTTATGAAC GTGGTCAGCG TTATGCAAGC CGATTGCAGA ATGAATTTGC TGGAAATATT TCTGCGCTGG CTGATGCGGA AAATATTTCA CGTAAGATTA TTACCCGCTG TATCAACACC GCCAAATTGC CTAAATCAGT TGTTGCTCTT TTTTCTCACC CCGGTGAACT ATCTGCCCGG TCAGGTGATG CACTTCAAAA AGCCTTTACA GATAAAGAGG AATTACTTAA GCAGCAGGCA TCTAACCTTC ATGAGCAGAA AAAAGCTGGG GTGATATTTG AAGCTGAAGA AGTTATCACT CTTTTAACTT CTGTGCTTAA AACGTCATCT GCATCAAGAA CTAGTTTAAG CTCACGACAT CAGTTTGCTC CTGGAGCGAC AGTATTGTAT AAGGGCGATA AAATGGTGCT TAACCTGGAC AGGTCTCGTG TTCCAACTGA GTGTATAGAG AAAATTGAGG CCATTCTTAA GGAACTTGAA AAGCCAGCAC CCTGATGCGA CCACGTTTTA GTCTACGTTT ATCTGTCTTT ACTTAATGTC CTTTGTTACA GGCCAGAAAG CATAACTGGC CTGAATATTC TCTCTGGGCC CACTGTTCCA CTTGTATCGT CGGTCTGATA ATCAGACTGG GACCACGGTC CCACTCGTAT CGTCGGTCTG ATTATTAGTC TGGGACCACG GTCCCACTCG TATCGTCGGT CTGATTATTA GTCTGGGACC ACGGTCCCAC TCGTATCGTC GGTCTGATAA TCAGACTGGG ACCACGGTCC CACTCGTATC GTCGGTCTGA TTATTAGTCT GGGACCATGG TCCCACTCGT ATCGTCGGTC TGATTATTAG TCTGGGACCA CGGTCCCACT CGTATCGTCG GTCTGATTAT TAGTCTGGAA CCACGGTCCC ACTCGTATCG TCGGTCTGAT TATTAGTCTG GGACCACGGT CCCACTCGTA TCGTCGGTCT GATTATTAGT CTGGGACCAC GATCCCACTC GTGTTGTCGG TCTGATTATC GGTCTGGGAC CACGGTCCCA CTTGTATTGT CGATCAGACT ATCAGCGTGA GACTACGATT CCATCAATGC CTGTCAAGGG CAAGTATTGA CATGTCGTCG TAACCTGTAG AACGGAGTAA CCTCGGTGTG CGGTTGTATG CCTGCTGTGG ATTGCTGCTG TGTCCTGCTT ATCCACAACA TTTTGCGCAC GGTTATGTGG ACAAAATACC TGGTTACCCA GGCCGTGCCG GCACGTTAAC CGGGCTGCAT CCGATGCAAG TGTGTCGCTG TCGACGAGCT CGCGAGCTCG GACATGAGGT TGCCCCGTAT TCAGTGTCGC TGATTTGTAT TGTCTGAAGT TGTTTTTACG TTAAGTTGAT GCAGATCAAT TAATACGATA CCTGCGTCAT AATTGATTAT TTGACGTGGT TTGATGGCCT CCACGCACGT TGTGATATGT AGATGATAAT CATTATCACT TTACGGGTCC TTTCCGGTGA TCCGACAGGT TACGGGGCGG CGACCTCGCG GGTTTTCGCT ATTTATGAAA ATTTTCCGGT TTAAGGCGTT TCCGTTCTTC TTCGTCATAA CTTAATGTTT TTATTTAAAA TACCCTCTGA AAAGAAAGGA AACGACAGGT GCTGAAAGCG AGCTTTTTGG CCTCTGTCGT TTCCTTTCTC TGTTTTTGTC CGTGGAATGA ACAATGGAAG TCCGAGCTCA TCGCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTCGAT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
