pAdTrack
$99.00
In stock
SKU
PL82143
100μg liquid plasmid(Instantly):$120
| name | pAdTrack | another name | apAdTrack |
| Insert Size (bp) | Plasmid host | Mammalian cell,Adenovirus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | pEGFP-N-3： | |
| 3′ sequencing primer | Forward(left ITR)： | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
nTTAATTAAn nnTCCCTTCC AGCTCTCTGC CCCTTTTGGA TTGAAGCCAA TATGATAATG AGGGGGTGGA GTTTGTGACG TGGCGCGGGG CGTGGGAACG GGGCGGGTGA CGTAGTAGTG TGGCGGAAGT GTGATGTTGC AAGTGTGGCG GAACACATGT AAGCGACGGA TGTGGCAAAA GTGACGTTTT TGGTGTGCGC CGGTGTACAC AGGAAGTGAC AATTTTCGCG CGGTTTTAGG CGGATGTTGT AGTAAATTTG GGCGTAACCG AGTAAGATTT GGCCATTTTC GCGGGAAAAC TGAATAAGAG GAAGTGAAAT CTGAATAATT TTGTGTTACT CATAGCGCGT AAnnnCGCGT TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCAG TTATCTAGAT CCGGTGGATC TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG GACTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG GTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT AGCGCTAGCG GATCTGACGG TTCACTAAAC CAGCTCTGCT TATATAGACC TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCAAGTAGG AAAGTCCCAT AAGGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC GTCAATAGGG GGCGTACTTG GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA CCGTAAATAC TCCACCCATT GACGTCAATG GAAAGTCCCT ATTGGCGTTA CTATGGGAAC ATACGTCATT ATTGACGTCA ATGGGCGGGG GTCGTTGGGC GGTCAGCCAG GCGGGCCATT TACCGTAAGT TATGTAACGC GGAACTCCAT ATATGGGCTA TGAACTAATG ACCCCGTAAT TGATTACTAT TAGATCTGGT ACCGTCGACG CGGCCGCGAT ATCCTCGAGA AGCTTTCTAG AGGATCTGGG CGTGGTTAAG GGTGGGAAAG AATATATAAG GTGGGGGTCT TATGTAGTTT TGTATCTGTT TTGCAGCAGC CGCCGCCGCC ATGAGCACCA ACTCGTTTGA TGGAAGCATT GTGAGCTCAT ATTTGACAAC GCGCATGCCC CCATGGGCCG GGGTGCGTCA GAATGTGATG GGCTCCAGCA TTGATGGTCG CCCCGTCCTG CCCGCAAACT CTACTACCTT GACCTACGAG ACCGTGTCTG GAACGCCGTT GGAGACTGCA GCCTCCGCCG CCGCTTCAGC CGCTGCAGCC ACCGCCCGCG GGATTGTGAC TGACTTTGCT TTCCTGAGCC CGCTTGCAAG CAGTGCAGCT TCCCGTTCAT CCGCCCGCGA TGACAAGTTG ACGGCTCTTT TGGCACAATT GGATTCTTTG ACCCGGGAAC TTAATGTCGT TTCTCAGCAG CTGTTGGATC TGCGCCAGCA GGTTTCTGCC CTGAAGGCTT CCTCCCCTCC CAATGCGGTT TAAAACATAA ATAAAAAACC AGACTCTGTT TGGATTTGGA TCAAGCAAGT GTCTTGCTGT CTTTATTTAG GGGTTTTGCG CGCGCGGTAG GCCCGGGACC AGCGGTCTCG GTCGTTGAGG GTCCTGTGTA TTTTTTCCAG GACGTGGTAA AGGTGACTCT GGATGTTCAG ATACATGGGC ATAAGCCCGT CTCTGGGGTG GAGGTAGCAC CACTGCAGAG CTTCATGCTG CGGGGTGGTG TTGTAGATGA TCCAGTCGTA GCAGGAGCGC TGGGCGTGGT GCCTAAAAAT GTCTTTCAGT AGCAAGCTGA TTGCCAGGGG CAGGCCCTTG GTGTAAGTGT TTACAAAGCG GTTAAGCTGG GATGGGTGCA TACGTGGGGA TATGAGATGC ATCTTGGACT GTATTTTTAG GTTGGCTATG TTCCCAGCCA TATCCCTCCG GGGATTCATG TTGTGCAGAA CCACCAGCAC AGTGTATCCG GTGCACTTGG GAAATTTGTC ATGTAGCTTA GAAGGAAATG CGTGGAAGAA CTTGGAGACG CCCTTGTGAC CTCCAAGATT TTCCATGCAT TCGTCCATAA TGATGGCAAT GGGCCCACGG GCGGCGGCCT GGGCGAAGAT ATTTCTGGGA TCACTAACGT CATAGTTGTG TTCCAGGATG AGATCGTCAT AGGCCATTTT TACAAAGCGC GGGCGGAGGG TGCCAGACTG CGGTATAATG GTTCCATCCG GCCCAGGGGC GTAGTTACCC TCACAGATTT GCATTTCCCA CGCTTTGAGT TCAGATGGGG GGATCATGTC TACCTGCGGG GCGATGAAGA AAACGGTTTC CGGGGTAGGG GAGATCAGCT GGGAAGAAAG CAGGTTCCTG AGCAGCTGCG ACTTACCGCA GCCGGTGGGC CCGTAAATCA CACCTATTAC CGGGTGCAAC TGGTAGTTAA GAGAGCTGCA GCTGCCGTCA TCCCTGAGCA GGGGGGCCAC TTCGTTAAGC ATGTCCCTGA CTCGCATGTT TTCCCTGACC AAATCCGCCA GAAGGCGCTC GCCGCCCAGC GATAGCAGTT CTTGCAAGGA AGCAAAGTTT TTCAACGGTT TGAGACCGTC CGCCGTAGGC ATGCTTTTGA GCGTTTGACC AAGCAGTTCC AGGCGGTCCC ACAGCTCGGT CACCTGCTCT ACGGCATCTC GATCCAGCAT ATCTCCTCGT TTCGCGGGTT GGGGCGGCTT TCGCTGTACG GCAGTAGTCG GTGCTCGTCC AGACGGGCCA GGGTCATGTC TTTCCACGGG CGCAGGGTCC TCGTCAGCGT AGTCTGGGTC ACGGTGAAGG GGTGCGCTCC GGGCTGCGCG CTGGCCAGGG TGCGCTTGAG GCTGGTCCTG CTGGTGCTGA AGCGCTGCCG GTCTTCGCCC TGCGCGTCGG CCAGGTAGCA TTTGACCATG GTGTCATAGT CCAGCCCCTC CGCGGCGTGG CCCTTGGCGC GCAGCTTGCC CTTGGAGGAG GCGCCGCACG AGGGGCAGTG CAGACTTTTG AGGGCGTAGA GCTTGGGCGC GAGAAATACC GATTCCGGGG AGTAGGCATC CGCGCCGCAG GCCCCGCAGA CGGTCTCGCA TTCCACGAGC CAGGTGAGCT CTGGCCGTTC GGGGTCAAAA ACCAGGTTTC CCCCATGCTT TTTGATGCGT TTCTTACCTC TGGTTTCCAT GAGCCGGTGT CCACGCTCGG TGACGAAAAG GCTGTCCGTG TCCCCGTATA CAGACTnnnG TTTAAACGAA TTCnnnTATA AAATGCAAGG TGCTGCTCAA AAAATCAGGC AAAGCCTCGC GCAAAAAAGA AAGCACATCG TAGTCATGCT CATGCAGATA AAGGCAGGTA AGCTCCGGAA CCACCACAGA AAAAGACACC ATTTTTCTCT CAAACATGTC TGCGGGTTTC TGCATAAACA CAAAATAAAA TAACAAAAAA ACATTTAAAC ATTAGAAGCC TGTCTTACAA CAGGAAAAAC AACCCTTATA AGCATAAGAC GGACTACGGC CATGCCGGCG TGACCGTAAA AAAACTGGTC ACCGTGATTA AAAAGCACCA CCGACAGCTC CTCGGTCATG TCCGGAGTCA TAATGTAAGA CTCGGTAAAC ACATCAGGTT GATTCATCGG TCAGTGCTAA AAAGCGACCG AAATAGCCCG GGGGAATACA TACCCGCAGG CGTAGAGACA ACATTACAGC CCCCATAGGA GGTATAACAA AATTAATAGG AGAGAAAAAC ACATAAACAC CTGAAAAACC CTCCTGCCTA GGCAAAATAG CACCCTCCCG CTCCAGAACA ACATACAGCG CTTCACAGCG GCAGCCTAAC AGTCAGCCTT ACCAGTAAAA AAGAAAACCT ATTAAAAAAA CACCACTCGA CACGGCACCA GCTCAATCAG TCACAGTGTA AAAAAGGGCC AAGTGCAGAG CGAGTATATA TAGGACTAAA AAATGACGTA ACGGTTAAAG TCCACAAAAA ACACCCAGAA AACCGCACGC GAACCTACGC CCAGAAACGA AAGCCAAAAA ACCCACAACT TCCTCAAATC GTCACTTCCG TTTTCCCACG TTACGTAACT TCCCATTTTA AGAAAACTAC AATTCCCAAC ACATACAAGT TACTCCGCCC TAAAACCTAC GTCACCCGCC CCGTTCCCAC GCCCCGCGCC ACGTCACAAA CTCCACCCCC TCATTATCAT ATTGGCTTCA ATCCAAAATA AGGTATATTA TTGATGATnn nTTAATTAAG GATCCnnnCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGnn nnnnAAAAAG GATCTTCACC TAGATCCTTT TCACGTAGAA AGCCAGTCCG CAGAAACGGT GCTGACCCCG GATGAATGTC AGCTACTGGG CTATCTGGAC AAGGGAAAAC GCAAGCGCAA AGAGAAAGCA GGTAGCTTGC AGTGGGCTTA CATGGCGATA GCTAGACTGG GCGGTTTTAT GGACAGCAAG CGAACCGGAA TTGCCAGCTG GGGCGCCCTC TGGTAAGGTT GGGAAGCCCT GCAAAGTAAA CTGGATGGCT TTCTCGCCGC CAAGGATCTG ATGGCGCAGG GGATCAAGCT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAATT TTGTTAAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAACAT CCCTTATAAA TCAAAAGAAT AGACCGCGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCAA ATCAAGTTTT TTGCGGTCGA GGTGCCGTAA AGCTCTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCGCGC TTAATGCGCC Gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnn
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