pDEST22
$99.00
In stock
SKU
PL82142
100μg liquid plasmid(Instantly):$120
| name | pDEST22 | another name | apDEST22 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | hybridization | Fragment type | GateWay |
| Fragment species | Prokaryotic resistance | Amp,Chl | |
| Selective marker | TRP1 | Fluorescent labeling | |
| Promoter | Replicon | pBR322 | |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CTCGAAATTA ACCCTCACTA AAGGGAACAA AAGCTGGTAC CGGGCCCCCC CTCGAGATCC GGGATCGAAG AAATGATGGT AAATGAAATA GGAAATCAAG GAGCATGAAG GCAAAAGACA AATATAAGGG TCGAACGAAA AATAAAGTGA AAAGTGTTGA TATGATGTAT TTGGCTTTGC GGCGCCGAAA AAACGAGTTT ACGCAATTGC ACAATCATGC TGACTCTGTG GCGGACCCGC GCTCTTGCCG GCCCGGCGAT AACGCTGGGC GTGAGGCTGT GCCCGGCGGA GTTTTTTGCG CCTGCATTTT CCAAGGTTTA CCCTGCGCTA AGGGGCGAGA TTGGAGAAGC AATAAGAATG CCGGTTGGGG TTGCGATGAT GACGACCACG ACAACTGGTG TCATTATTTA AGTTGCCGAA AGAACCTGAG TGCATTTGCA ACATGAGTAT ACTAGAAGAA TGAGCCAAGA CTTGCGAGAC GCGAGTTTGC CGGTGGTGCG AACAATAGAG CGACCATGAC CTTGAAGGTG AGACGCGCAT AACCGCTAGA GTACTTTGAA GAGGAAACAG CAATAGGGTT GCTACCAGTA TAAATAGACA GGTACATACA ACACTGGAAA TGGTTGTCTG TTTGAGTACG CTTTCAATTC ATTTGGGTGT GCACTTTATT ATGTTACAAT ATGGAAGGGA ACTTTACACT TCTCCTATGC ACATATATTA ATTAAAGTCC AATGCTAGTA GAGAAGGGGG GTAACACCCC TCCGCGCTCT TTTCCGATTT TTTTCTAAAC CGTGGAATAT TTCGGATATC CTTTTGTTGT TTCCGGGTGT ACAATATGGA CTTCCTCTTT TCTGGCAACC AAACCCATAC ATCGGGATTC CTATAATACC TTCGTTGGTC TCCCTAACAT GTAGGTGGCG GAGGGGAGAT ATACAATAGA ACAGATACCA GACAAGACAT AATGGGCTAA ACAAGACTAC ACCAATTACA CTGCCTCATT GATGGTGGTA CATAACGAAC TAATACTGTA GCCCTAGACT TGATAGCCAT CATCATATCG AAGTTTCACT ACCCTTTTTC CATTTGCCAT CTATTGAAGT AATAATAGGC GCATGCAACT TCTTTTCTTT TTTTTTCTTT TCTCTCTCCC CCGTTGTTGT CTCACCATAT CCGCAATGAC AAAAAAATGA TGGAAGACAC TAAAGGAAAA AATTAACGAC AAAGACAGCA CCAACAGATG TCGTTGTTCC AGAGCTGATG AGGGGTATCT CGAAGCACAC GAAACTTTTT CCTTCCTTCA TTCACGCACA CTACTCTCTA ATGAGCAACG GTATACGGCC TTCCTTCCAG TTACTTGAAT TTGAAATAAA AAAAAGTTTG CTGTCTTGCT ATCAAGTATA AATAGACCTG CAATTATTAA TCTTTTGTTT CCTCGTCATT GTTCTCGTTC CCTTTCTTCC TTGTTTCTTT TTCTGCACAA TATTTCAAGC TATACCAAGC ATACAATCAA CTCCAAGCTT ATGCCCAAGA AGAAGCGGAA GGTCTCGAGC GGCGCCAATT TTAATCAAAG TGGGAATATT GCTGATAGCT CATTGTCCTT CACTTTCACT AACAGTAGCA ACGGTCCGAA CCTCATAACA ACTCAAACAA ATTCTCAAGC GCTTTCACAA CCAATTGCCT CCTCTAACGT TCATGATAAC TTCATGAATA ATGAAATCAC GGCTAGTAAA ATTGATGATG GTAATAATTC AAAACCACTG TCACCTGGTT GGACGGACCA AACTGCGTAT AACGCGTTTG GAATCACTAC AGGGATGTTT AATACCACTA CAATGGATGA TGTATATAAC TATCTATTCG ATGATGAAGA TACCCCACCA AACCCAAAAA AAGAGGGTGG GTCGAATCAA ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA TATCCAGTCA CTATGGCGGC CGCTAAGTTG GCAGCATCAC CCGACGCACT TTGCGCCGAA TAAATACCTG TGACGGAAGA TCACTTCGCA GAATAAATAA ATCCTGGTGT CCCTGTTGAT ACCGGGAAGC CCTGGGCCAA CTTTTGGCGA AAATGAGACG TTGATCGGCA CGTAAGAGGT TCCAACTTTC ACCATAATGA AATAAGATCA CTACCGGGCG TATTTTTTGA GTCATCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC CGTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC CGCTGGCGAT TCAGGTTCAT CATGCCGTTT GTGATGGCTT CCATGTCGGC AGAATGCTTA ATGAATTACA ACAGTACTGC GATGAGTGGC AGGCGGGGCG TAATCTAGAG GATCCGGCTT ACTAAAAGCC AGATAACAGT ATGCGTATTT GCGCGCTGAT TTTTGCGGTA TAAGAATATA TACTGATATG TATACCCGAA GTATGTCAAA AAGAGGTATG CTATGAAGCA GCGTATTACA GTGACAGTTG ACAGCGACAG CTATCAGTTG CTCAAGGCAT ATATGATGTC AATATCTCCG GTCTGGTAAG CACAACCATG CAGAATGAAG CCCGTCGTCT GCGTGCCGAA CGCTGGAAAG CGGAAAATCA GGAAGGGATG GCTGAGGTCG CCCGGTTTAT TGAAATGAAC GGCTCTTTTG CTGACGAGAA CAGGGGCTGG TGAAATGCAG TTTAAGGTTT ACACCTATAA AAGAGAGAGC CGTTATCGTC TGTTTGTGGA TGTACAGAGT GATATTATTG ACACGCCCGG GCGACGGATG GTGATCCCCC TGGCCAGTGC ACGTCTGCTG TCAGATAAAG TCCCCCGTGA ACTTTACCCG GTGGTGCATA TCGGGGATGA AAGCTGGCGC ATGATGACCA CCGATATGGC CAGTGTGCCG GTCTCCGTTA TCGGGGAAGA AGTGGCTGAT CTCAGCCACC GCGAAAATGA CATCAAAAAC GCCATTAACC TGATGTTCTG GGGAATATAA ATGTCAGGCT CCCTTATACA CAGCCAGTCT GCAGGTCGAC CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT CTTGTACAAA GTGGTTTGAT GGCCGCTAAG TAAGTAAGAC GTCGAGCTCT AAGTAAGTAA CGGCCGCCAC CGCGGTGGAG CTTTGGACTT CTTCGCCAGA GGTTTGGTCA AGTCTCCAAT CAAGGTTGTC GGCTTGTCTA CCTTGCCAGA AATTTACGAA AAGATGGAAA AGGGTCAAAT CGTTGGTAGA TACGTTGTTG ACACTTCTAA ATAAGCGAAT TTCTTATGAT TTATGATTTT TATTATTAAA TAAGTTATAA AAAAAATAAG TGTATACAAA TTTTAAAGTG ACTCTTAGGT TTTAAAACGA AAATTCTTAT TCTTGAGTAA CTCTTTCCTG TAGGTCAGGT TGCTTTCTCA GGTATAGCAT GAGGTCGCTC TTATTGACCA CACCTCTACC GGCATGCCGA GCAAATGCCT GCAAATCGCT CCCCATTTCA CCCAATTGTA GATATGCTAA CTCCAGCAAT GAGTTGATGA ATCTCGGTGT GTATTTTATG TCCTCAGAGG ACAACACCTG TTGTAATCGT TCTTCCACAC GGATCCCAAT TCGCCCTATA GTGAGTCGTA TTACAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATAGATCGG CAAGTGCACA AACAATACTT AAATAAATAC TACTCAGTAA TAACCTATTT CTTAGCATTT TTGACGAAAT TTGCTATTTT GTTAGAGTCT TTTACACCAT TTGTCTCCAC ACCTCCGCTT ACATCAACAC CAATAACGCC ATTTAATCTA AGCGCATCAC CAACATTTTC TGGCGTCAGT CCACCAGCTA ACATAAAATG TAAGCTTTCG GGGCTCTCTT GCCTTCCAAC CCAGTCAGAA ATCGAGTTCC AATCCAAAAG TTCACCTGTC CCACCTGCTT CTGAATCAAA CAAGGGAATA AACGAATGAG GTTTCTGTGA AGCTGCACTG AGTAGTATGT TGCAGTCTTT TGGAAATACG AGTCTTTTAA TAACTGGCAA ACCGAGGAAC TCTTGGTATT CTTGCCACGA CTCATCTCCA TGCAGTTGGA CGATATCAAT GCCGTAATCA TTGACCAGAG CCAAAACATC CTCCTTAGGT TGATTACGAA ACACGCCAAC CAAGTATTTC GGAGTGCCTG AACTATTTTT ATATGCTTTT ACAAGACTTG AAATTTTCCT TGCAATAACC GGGTCAATTG TTCTCTTTCT ATTGGGCACA CATATAATAC CCAGCAAGTC AGCATCGGAA TCTAGAGCAC ATTCTGCGGC CTCTGTGCTC TGCAAGCCGC AAACTTTCAC CAATGGACCA GAACTACCTG TGAAATTAAT AACAGACATA CTCCAAGCTG CCTTTGTGTG CTTAATCACG TATACTCACG TGCTCAATAG TCACCAATGC CCTCCCTCTT GGCCCTCTCC TTTTCTTTTT TCGACCGAAT TAATTCTTAA TCGGCAAAAA AAGAAAAGCT CCGGATCAAG ATTGTACGTA AGGTGACAAG CTATTTTTCA ATAAAGAATA TCTTCCACTA CTGCCATCTG GCGTCATAAC TGCAAAGTAC ACATATATTA CGATGCTGTT CTATTAAATG CTTCCTATAT TATATATATA GTAATGTCGT GATCTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGGA TCGCTTGCCT GTAACTTACA CGCGCCTCGT ATCTTTTAAT GATGGAATAA TTTGGGAATT TACTCTGTGT TTATTTATTT TTATGTTTTG TATTTGGATT TTAGAAAGTA AATAAAGAAG GTAGAAGAGT TACGGAATGA AGAAAAAAAA ATAAACAAAG GTTTAAAAAA TTTCAACAAA AAGCGTACTT TACATATATA TTTATTAGAC AAGAAAAGCA GATTAAATAG ATATACATTC GATTAACGAT AAGTAAAATG TAAAATCACA GGATTTTCGT GTGTGGTCTT CTACACAGAC AAGATGAAAC AATTCGGCAT TAATACCTGA GAGCAGGAAG AGCAAGATAA AAGGTAGTAT TTGTTGGCGA TCCCCCTAGA GTCTTTTACA TCTTCGGAAA ACAAAAACTA TTTTTTCTTT AATTTCTTTT TTTACTTTCT ATTTTTAATT TATATATTTA TATTAAAAAA TTTAAATTAT AATTATTTTT ATAGCACGTG ATGAAAAGGA CCCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
