pDG148
$128.00
In stock
SKU
PL82139
100μg liquid plasmid(Instantly):$163
| name | pDG148 | another name | apDG148 |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGGGAAAAAC GGGAAGTAGG CTCCCATGAT AAAAAAGTAA AAGAAAAAGA ATAAACCGAA CATCCAAAAG TTTGTGTTTT TTAAATAGTA CATAATGGAT TTCCTTACGC GAAATACGGG CAGACATGGC CTGCCCGGTT ATTATTATTT TTGACACCAG ACCAACTGGT AATGGTAGCG ACCGGCGCTC AGCTGGAATT CTACACAGCC CAGTCCAGAC TATTCGGCAC TGAAATTATG GGTGAAGTGG TCAAGACCTC ACTAGGCACC TTAAAAATAG CGCACCCTGA AGAAGATTTA TTTGAGGTAG CCCTTGCCTA CCTAGCTTCC AAGAAAGATA TCCTAACAGC ACAAGAGCGG AAAGATGTTT TGTTCTACAT CCAGAACAAC CTCTGCTAAA ATTCCTGAAA AATTTTGCAA AAAGTTGTTG ACTTTATCTA CAAGGTGTGG CATAATGTGT GGAATTGTGA GCGGATAACA ATTAAGCTTA AGGAGGTGAT CTAGAGTCGA CCTGCAGGCA TGCAAGCTAA TTCGGTGGAA ACGAGGTCAT CATTTCCTTC CGAAAAAACG GTTGCATTTA AATCTTACAT ATGTAATACT TTCAAAGACT ACATTTGTAA GATTTGATGT TTGAGTCGGC TGAAAGATCG TACGTACCAA TTATTGTTTC GTGATTGTTC AAGCCATAAC ACTGTAGGGA TAGTGGAAAG AGTGCTTCAT CTGGTTACGA TCAATCAAAT ATTCAAACGG AGGGAGACGA TTTTGATGAA ACCAGTAACG TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA GCCTGTAAAA CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAAGAC GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT TAAGTTCTGT CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA TAAATATCTC ACTCGCAATC AAATTCAGCC GATAGCGGAA CGGGAAGGCG ACTGGAGTGC CATGTCCGGT TTTCAACAAA CCATGCAAAT GCTGAATGAG GGCATCGTTC CCACTGCGAT GCTGGTTGCC AACGATCAGA TGGCGCTGGG CGCAATGCGC GCCATTACCG AGTCCGGGCT GCGCGTTGGT GCGGATATCT CGGTAGTGGG ATACGACGAT ACCGAAGACA GCTCATGTTA TATCCCGCCG TTAACCACCA TCAAACAGGA TTTTCGCCTG CTGGGGCAAA CCAGCGTGGA CCGCTTGCTG CAACTCTCTC AGGGCCAGGC GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGGTGAAA AGAAAAACCA CCCTGGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGGATCCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTG CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA CACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTTCAAG AATTCCTTAA GGAACGTACA GACGGCTTAA AAGCCTTTAA AAACGTTTTT AAGGGGTTTG TAGACAAGGT AAAGGATAAA ACAGCACAAT TCCAAGAAAA ACACGATTTA GAACCTAAAA AGAACGAATT TGAACTAACT CATAACCGAG AGGTAAAAAA AGAACGAAGT CGAGATCAGG GAATGAGTTT ATAAAATAAA AAAAGCACCT GAAAAGGTGT CTTTTTTTGA TGGTTTTGAA CTTGTTCTTT CTTATCTTGA TACATATAGA AATAACGTCA TTTTTATTTT AGTTGCTGAA AGGTGCGTTG AAGTGTTGGT ATGTATGTGT TTTAAAGTAT TGAAAACCCT TAAAATTGGT TGCACAGAAA AACCCCATCT GTTAAAGTTA TAAGTGACTA AACAAATAAC TAAATAGATG GGGTTTTCTT TTAATATTAT GTGTCCTAAT AGTAGCATTT ATTCAGATGA AAAATCAAGG GTTTTAGTGG ACAAGACAAA AAGTGGAAAA GTGAGACCAT GGAGAGAAAA GAAAATCGCT AATGTTGATT ACTTTGAACT TCTGCATATT CTTGAATTTA AAAAGGCTGA AAGAGTAAAA GATTGTGCTG AAATATTAGA GTATAAACAA AATCGTGAAA CAGGCGAAAG AAAGTTGTAT CGAGTGTGGT TTTGTAAATC CAGGCTTTGT CCAATGTGCA ACTGGAGGAG AGCAATGAAA CATGGCATTC AGTCACAAAA GGTTGTTGCT GAAGTTATTA AACAAAAGCC AACAGTTCGT TGGTTGTTTC TCACATTAAC AGTTAAAAAT GTTTATGATG GCGAAGAATT AAATAAGAGT TTGTCAGATA TGGCTCAAGG ATTTCGCCGA ATGATGCAAT ATAAAAAAAT TAATAAAAAT CTTGTTGGTT TTATGCGTGC AACGGAAGTG ACAATAAATA ATAAAGATAA TTCTTATAAT CAGCACATGC ATGTATTGGT ATGTGTGGAA CCAACTTATT TTAAGAATAC AGAAAACTAC GTGAATCAAA AACAATGGAT TCAATTTTGG AAAAAGGCAA TGAAATTAGA CTATGATCCA AATGTAAAAG TTCAAATGAT TCGACCGAAA AATAAATATA AATCGGATAT ACAATCGGCA ATTGACGAAA CTGCAAAATA TCCTGTAAAG GATACGGATT TTATGACCGA TGATGAAGAA AAGAATTTGA AACGTTTGTC TGATTTGGAG GAAGGTTTAC ACCGTAAAAG GTTAATCTCC TATGGTGGTT TGTTAAAAGA AATACATAAA AAATTAAACC TTGATGACAC AGAAGAAGGC GATTTGATTC ATACAGATGA TGACGAAAAA GCCGATGAAG ATGGATTTTC TATTATTGCA ATGTGGAATT GGGAACGGAA AAATTATTTT ATTAAAGAGT AGTTCAACAA ACGGGCCAGT TTGTTGAAGA TTAGATGCTA TAATTGTTAT TAAAAGGATT GAAGGATGCT TAGGAAGACG AGTTATTAAT AGCTGAATAA GAACGGTGCT CTCCAAATAT TCTTATTTAG AAAAGCAAAT CTAAAATTAT CTGAAAAGGG AATGAGAATA GTGAATGGAC CAATAATAAT GACTAGAGAA GAAAGAATGA AGATTGTTCA TGAAATTAAG GAACGAATAT TGGATAAATA TGGGGATGAT GTTAAGGCTA TTGGTGTTTA TGGCTCTCTT GGTCGTCAGA CTGATGGGCC CTATTCGGAT ATTGAGATGA TGTGTGTCAT GTCAACAGAG GAAGCAGAGT TCAGCCATGA ATGGACAACC GGTGAGTGGA AGGTGGAAGT GAATTTTGAT AGCGAAGAGA TTCTACTAGA TTATGCATCT CAGGTGGAAT CAGATTGGCC GCTTACACAT GGTCAATTTT TCTCTATTTT GCCGATTTAT GATTCAGGTG GATACTTAGA GAAAGTGTAT CAAACTGCTA AATCGGTAGA AGCCCAAACG TTCCACGATG CGATTTGTGC CCTTATCGTA GAAGAGCTGT TTGAATATGC AGGCAAATGG CGTAATATTC GTGTGCAAGG ACCGACAACA TTTCTACCAT CCTTGACTGT ACAGGTAGCA ATGGCAGGTG CCATGTTGAT TGGTCTGCAT CATCGCATCT GTTATACGAC GAGCGCTTCG GTCTTAACTG AAGCAGTTAA GCAATCAGAT CTTCCTTCAG GTTATGACCA TCTGTGCCAG TTCGTAATGT CTGGTCAACT TTCCGACTCT GAGAAACTTC TGGAATCGCT AGAGAATTTC TGGAATGGGA TTCAGGAGTG GACAGAACGA CACGGATATA TAGTGGATGT GTCAAAACGC ATACCATTTT GAACGATGAC CTCTAATAAT TGTTAATCAT GTTGGTTACG TATTTATTAA CTTCTCCTAG TATTAGTAAT TATCATGGCT GTCATGGCGC ATTAACGGAA TAAAGGGTGT GCTTAAATCG GGCCATTTTG CGTAATAAGA AAAAGGATTA ATTATGAGCG AATTGAATTA ATAATAAGGT AATAGATTTA CATTAGAAAA TGAAAGGGGA TTTTATGCGT GAGAATGTTA CAGTCTATCC CGGCATTGCC AGTCGGGGAT ATTAAAAAGA GTATAGGTTT TTATTGCGAT AAACTAGGTT TCACTTTGGT TCACCATGAA GATGGATTCG CAGTTCTAAT GTGTAATGAG GTTCGGATTC ATCTATGGGA GGCAAGTGAT GAAGGCTGGC GCTCTCGTAG TAATGATTCA CCGGTTTGTA CAGGTGCGGA GTCGTTTATT GCTGGTACTG CTAGTTGCCG CATTGAAGTA GAGGGAATTG ATGAATTATA TCAACATATT AAGCCTTTGG GCATTTTGCA CCCCAATACA TCATTAAAAG ATCAGTGGTG GGATGAACGA GACTTTGCAG TAATTGATCC CGACAACAAT TTGATTAGCT TTTTTCAACA AATAAAAAGC TAAAATCTAT TATTAATCTG TTCAGCAATC GGGCGCGATT GCTGAATAAA AGATACGAGA GACCTCTCTT GTATCTTTTT TATTTTGAGT GGTTTTGTCC GTTACACTAG AAAACCGAAA GACAATAAAA ATTTTATTCT TGCTGAGTCT GGCTTTCGGT AAGCTAGACA AAACGGACAA AATAAAAATT GGCAAGGGTT TAAAGGTGGA GATTTTTTGA GTGATCTTCT CAAAAAATAC TACCTGTCCC TTGCTGATTT TTAAACGAGC ACGAGAGCAA AACCCCCCTT TGCTGAGGTG GCGAACGATG AGCCAATAAT ACGTACAGAC ATAATAGTGC CAGCCAGCAG CAGGGCGTTT TTCCCACCGA TGCGATTAAT GATCAGTGGC GCAAAGAACA TAATCGAGGC GTTAAGTAAT TCGCCCATTG TCGTTACGTA GCCAAATACC CGCGTACCCT GTTCACCGGT AGCAAAGAAC GAAGTAAAGA AATTAGCAAA CTGTTGGTCA AAAACATCGT AGGTGCAGGA AACGCCAATA ACATACAGTG ACAAAAACCA CAGTTTTGGC TGTCTGAACA GTTCCAGTGC CAGCTTAAGG CTAAATGCCG AATGGTTGGC ACCTACCGCA TTGGCAACCG TGGCAGAAGA GGGCGCATCC GTTTTGGCGA AAAAGAGTAA AACGGCGAGG ATGAGTGCAC AGCCAGAGCC CAGCCAGAAA ACAAACTGAT TATTGATGGT GAACATGATG CCGACAATCG AGGCACACAG CGCCCAGCCA ACACAGCCAA ACATCCGCGC GCGACCAAAT TCGAAATTAC TGCGACGGCT GACTTTCTCA ATAAATGCCT CTACTGCTGG CGCACCGGCG TTAAAACAAA AGCCTAGATA AATACCACCA ACAATCGATC CTACTAAAAT GTTGTATTGT AACAGTGGCC CGAAGATAAA AATAAAGAAC GGCGCAAACA TCACTAACAT GCCGGTAATA ATCCACAGCA GGTATTTGCG CAGCCCGAGT TTGTCAGAAA GCAGACCAAA CAGCGGTTGG AATAATAGCG AGAACAGAGA AATAGCGGCA AAAATAATAC CCGTATCACT TTTGCTGATA TGGTTGATGT CATGTAGCCA AATCGGGAAA AACGGGAAGT AGGCTCCCAT GATAAAAAAG TAAAAGAAAA AGAATAAACC GAACATCCAA AAGTTTGTGT TTTTTAAATA GTACATAATG GATTTCCTTA CGCGAAATAC GGGCAGACAT GGCCTGCCCG GTTATTATTA TTTTTGACAC CAGACCAACT GGTAATGGTA GCGACCGGCG CTCAGCTGGA ATTCTACACA GCCCAGTCCA GACTATTCGG CACTGAAATT ATGGGTGAAG TGGTCAAGAC CTCACTAGGC ACCTTAAAAA TAGCGCACCC TGAAGAAGAT TTATTTGAGG TAGCCCTTGC CTACCTAGCT TCCAAGAAAG ATATCCTAAC AGCACAAGAG CGGAAAGATG TTTTGTTCTA CATCCAGAAC AACCTCTGCT AAAATTCCTG AAAAATTTTG CAAAAAGTTG TTGACTTTAT CTACAAGGTG TGGCATAATG TGTGGAATTG TGAGCGGATA
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