pGZ
$99.00
In stock
SKU
PL82057
100μg liquid plasmid(Instantly):$120
| name | pGZ | another name | apGZ |
| Insert Size (bp) | Plasmid host | Mammary cell,Lentiviral | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CAGCTTGGCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGAATT TGGGCCGTCT CTGCAAAAGT CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATA GCTAGAGCCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTC CTCTCACTCT CTGATATTCA TTTCTTTGCA AGTTATAAAT ACTGAATAAT AAGATGACAT GAACTACTAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAAGC AAAAAGCAGA TCTTATTTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCGAGCT CGAATTAATT CCAGGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGATGC GGAAGAGGCC GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC AGCAGCGGGC AAGGCAGGCG GCGATGAGTT CCGCCGTGGC AATAGGGAGG GGGAAAGCGA AAGTCCCGGA AAGGAGCTGA CAGGTGGTGG CAATGCCCCA ACCAGTGGGG GTTGCGTCAG CAAACACAGT GCACACCACG CCACGTTGCC TGACAACGGG CCACAACTCC TCATAAAGAG ACAGCAACCA GGATTTATAC AAGGAGGAGA AAATGAAAGC CATACGGGAA GCAATAGCAT GATACAAAGG CATTAAAGCA GCGTATCCAC ATAGCGTAAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA ATTTTGTAAT CCAGAGGTTG ATTGTCGAGT CGACTAGTCC TGTTCCTCAG CTACAAAATG GACACAATTT CCAGCAGGGT CTCTGAGGGC AAATTCCCTT CCCCAAGGTT GTTCACCAAT TTCTGTCATG GCTGGGCCAG AGGCATCCCT GAAATTTGTG CTGACTACTT CTGACCATTC TGCATAAAGC TCATCTAGGC CTCTGACCCA GACCCAAGCA AGGGTGTTGT CAGGGACAAC TTGGTCCTGA ACTGCTGAGA TGAAGAGGGT GACATCATCT CTGACAACAC CAGCAAAATC ATCTTCAACA AAGTCTCTGG AGAATCCTAA TCTGTCAGTC CAGAACTCTA CAGCCCCTGC AACATCCCTT GCTGTGAGGA CTGGGACTGC AGAAGTGAGT TTGGCCATAC CGGAAGGGCC GGGATTCTCC TCCACGTCAC CGCATGTTAG AAGACTTCCT CTGCCCTCAC CGGTCACATT GATCCTAGCA GAAGCACAGG CTGCAGGGTG ACGGTCCATC CCGCTCTCCT GGGCACAAGA CATGGGCAGC GTGCCATCAT CCTGCGCCGC CACCGCCGGC GGGAAGCCAT GGCTGATATC TCGAGATCTG GCGAAGGCGA TGGGGGTCTT GAAGGCGTGC TGGTACTCCA CGATGCCCAG CTCGGTGTTG CTGTGCAGCT CCTCCACGCG GCGGAAGGCG AACATGGGGC CCCCGTTCTG CAGGATGCTG GGGTGGATGG CGCTCTTGAA GTGCATGTGG CTGTCCACCA CGAAGCTGTA GTAGCCGCCG TCGCGCAGGC TGAAGGTGCG GGCGAAGCTG CCCACCAGCA CGTTATCGCC CATGGGGTGC AGGTGCTCCA CGGTGGCGTT GCTGCGGATG ATCTTGTCGG TGAAGATCAC GCTGTCCTCG GGGAAGCCGG TGCCCACCAC CTTGAAGTCG CCGATCACGC GGCCGGCCTC GTAGCGGTAG CTGAAGCTCA CGTGCAGCAC GCCGCCGTCC TCGTACTTCT CGATGCGGGT GTTGGTGTAG CCGCCGTTGT TGATGGCGTG CAGGAAGGGG TTCTCGTAGC CGCTGGGGTA GGTGCCGAAG TGGTAGAAGC CGTAGCCCAT CACGTGGCTC AGCAGGTAGG GGCTGAAGGT CAGGGCGCCT TTGGTGCTCT TCATCTTGTT GGTCATGCGG CCCTGCTCGG GGGTGCCCTC TCCGCCGCCC ACCAGCTCGA ACTCCACGCC GTTCAGGGTG CCGGTGATGC GGCACTCGAT CTTCATGGCG GGCATGGTGG CGGATCCAAC GAATGTCGAG AGGCTGGATC GGTCCCGGTG TCTTCTATGG AGGTCAAAAC AGCGTGGATG GCGTCTCCAG GCGATCTGAC GGTTCACTAA ACGAGCTCTG CTTATATAGG CCTCCCACCG TACACGCCTA ACTAGTTCTA GAATTCATCG ATAAAATTTT GAATTTTGTA ATTTGTTTTT GTAATTCTTT AGTTTGTATG TCTGTTGCTA TTATGTCTAC TATTCTTTCC CCTGCACTGT ACCCCCCAAT CCCCCCTTTT CTTTTAAAAG TTAACCGATA CCGTCGAGAT CCGTTCACTA ATCGAATGGA TCTGTCTCTG TCTCTCTCTC CACCTTCTTC TTCTATTCCT TCGGGCCTGT CGGGTCCCCT CGGGGTTGGG AGGTGGGTCT GAAACGATAA TGGTGAATAT CCCTGCCTAA CTCTATTCAC TATAGAAAGT ACAGCAAAAA CTATTCTTAA ACCTACCAAG CCTCCTACTA TCATTATGAA TAATTTTATA TACCACAGCC AATTTGTTAT GTTAAACCAA TTCCACAAAC TTGCCCATTT ATCTAATTCC AATAATTCTT GTTCATTCTT TTCTTGCTGG TTTTGCGATT CTTCAATTAA GGAGTGTATT AAGCTTGTGT AATTGTTAAT TTCTCTGTCC CACTCCATCC AGGTCGTGTG ATTCCAATCT GTTCCAGAGA TTTATTACTC CAACTAGCAT TCCAAGGCAC AGCAGTGGTG CAAATGAGTT TTCCAGAGCA ACCCCAAATC CCCAGGAGCT GTTGATCCTT TAGGTATCTT TCCACAGCCA GGATTCTTGC CTGGAGCTGC TTGATGCCCC AGACTGTGAG TTGCAACAGA TGCTGTTGCG CCTCAATAGC CCTCAGCAAA TTGTTCTGCT GCTGCACTAT ACCAGACAAT AATTGTCTGG CCTGTACCGT CAGCGTCATT GAGGCTGCGC CCATAGTGCT TCCTGCTGCT CCCAAGAACC CAAGGAACAA AGCTCCTATT CCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGCTTGCTGT GCGGTGGTCT TACTTTTGTT TTGCTCTTCC TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA TCCTTTGATG CACACAATAG AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG ATCCTGTCTG AAGGGATGGT TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT CTAACAGGCC AGGATTAACT GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT TTAATTTATA TTTTTTCTTT CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA TTCTCCCCCG CTTAATACTG ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG TCAAAATTTT TGGCGTACTC ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG CAAGCCGAGT CCTGCGTCGA GAGAGCTCTG GTTTCCCTTT CGCTTTCAGG TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT GGTCTAACCA GAGAGACCCG TTTATTGTAT CGAGCTAGGC ACTTAAATAC AATATCTCTG CAATGCGGCA ATTCAGTGGT TCGTCCAATC CATGTCAGAC CCGTCTGTTG CCTTCCTAAT AAGGCACGAT CGTACCACCT TACTTCCACC AATCGGCATG CACGGTGCTT TTTCTCTCCT TGTAAGGCAT GTTGCTAACT CATCGTTACC ATGTTGCAAG ACTACAAGAG TATTGCATAA GACTACACGC GT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
