pAN8-1-gpdA
$157.00
In stock
SKU
PL82038
100μg liquid plasmid(Instantly):$178
| name | pAN8-1-gpdA | another name | apAN8-1-gpdA |
| Insert Size (bp) | Plasmid host | filiformFungus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | gpdA | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCCTTGTAT CTCTACACAC AGGCTCAAAT CAATAAGAAG AACGGTTCGT CTTTTTCGTT TATATCTTGC ATCGTCCCAA AGCTATTGGC GGGATATTCT GTTTGCAGTT GGCTGACTTG AAGTAATCTC TGCAGATCTT TCGACACTGA AATACGTCGA GCCTGCTCCG CTTGGAAGCG GCGAGGAGCC TCGTCCTGTC ACAACTACCA ACATGGAGTA CGATAAGGGC CAGTTCCGCC AGCTCATTAA GAGCCAGTTC ATGGGCGTTG GCATGATGGC CGTCATGCAT CTGTACTTCA AGTACACCAA CCCTCTTCTG ATCCAGTCGA TCATCCCGCT GAAGGGCGCT TTCGAATCGA ATCTGGTTAA GATCCACGTC TTCGGGAAGC CAGCGACTGG TGACCTCCAG CGTCCCTTTA AGGCTGCCAA CAGCTTTCTC AGCCAGGGCC AGCCCAAGAC CGACAAGGCC TCCCTCCAGA ACGCCGAGAA GAACTGGAGG GGTGGTGTCA AGGAGGAGTA AGCTCCTTAT TGAAGTCGGA GGACGGAGCG GTGTCAAGAG GATATTCTTC GCTCTGTATT ATAGATAAGA TGATGAGGAA TTGGAGGTAG CATAGCTTCA TTTGGATTTG CTTTCCAGGC TGAGACTCTA GCTTGGAGCA TAGAGGGTCC CTTTGGCTTT CAATATTCTC AAGTATCTCG AGTTTGAACT TATTCCCGTG AACCTTTTAT TCACCAATGA GCATTGGAAT GAACATGAAT CTGAGGACTG CAATCGCCAT GAGGTTTTCG AAATACATCC GGATGTCGAA GGCTTGGGGC ACCTGCGTTG GTTGAATTTA GAACGTGGCA CTATTGATCA TCCGATAGCT CTGCAAAGGG CGTTGCACAA TGCAAGTCAA ACGTTGCTAG CAGTTCCAGG TGGAATGTTA TGATGAGCAT TGTATTAAAT CAGGAGATAT AGCATGATCT CTAGTTAGCT CACCACAAAA GTCAGACGGC GTAACCAAAA GTCACACAAC ACAAGCTGTA AGGATTTCGG CACGGCTACG GAAGACGGAG AAGCCCACCT TCAGTGGACT CGAGTACCAT TTAATTCTAT TTGTGTTTGA TCGAGACCTA ATACAGCCCC TACAACGACC ATCAAAGTCG TATAGCTACC AGTGAGGAAG TGGACTCAAA TCGACTTCAG CAACATCTCC TGGATAAACT TTAAGCCTAA ACTATACAGA ATAAGATGGT GGAGAGCTTA TACCGAGCTC CCAAATCTGT CCAGATCATG GTTGACCGGT GCCTGGATCT TCCTATAGAA TCATCCTTAT TCGTTGACCT AGCTGATTCT GGAGTGACCC AGAGGGTCAT GACTTGAGCC TAAAATCCGC CGCCTCCACC ATTTGTAGAA AAATGTGACG AACTCGTGAG CTCTGTACAG TGACCGGTGA CTCTTTCTGG CATGCGGAGA GACGGACGGA CGCAGAGAGA AGGGCTGAGT AATAAGCGCC ACTGCGCCAG ACAGCTCTGG CGGCTCTGAG GTGCAGTGGA TGATTATTAA TCCGGGACCG GCCGCCCCTC CGCCCCGAAG TGGAAAGGCT GGTGTGCCCC TCGTTGACCA AGAATCTATT GCATCATCGG AGAATATGGA GCTTCATCGA ATCACCGGCA GTAAGCGAAG GAGAATGTGA AGCCAGGGGT GTATAGCCGT CGGCGAAATA GCATGCCATT AACCTAGGTA CAGAAGTCCA ATTGCTTCCG ATCTGGTAAA AGATTCACGA GATAGTACCT TCTCCGAAGT AGGTAGAGCG AGTACCCGGC GCGTAAGCTC CCTAATTGGC CCATCCGGCA TCTGTAGGGC GTCCAAATAT CGTGCCTCTC CTGCTTTGCC CGGTGTATGA AACCGGAAAG GCCGCTCAGG AGCTGGCCAG CGGCGCAGAC CGGGAACACA AGCTGGCAGT CGACCCATCC GGTGCTCTGC ACTCGACCTG CTGAGGTCCC TCAGTCCCTG GTAGGCAGCT TTGCCCCGTC TGTCCGCCCG GTGTGTCGGC GGGGTTGACA AGGTCGTTGC GTCAGTCCAA CATTTGTTGC CATATTTTCC TGCTCTCCCC ACCAGCTGCT CTTTTCTTTT CTCTTTCTTT TCCCATCTTC AGTATATTCA TCTTCCCATC CAAGAACCTT TATTTCCCCT AAGTAAGTAC TTTGCTACAT CCATACTCCA TCCTTCCCAT CCCTTATTCC TTGGAACCTT TCAGTTCGAG CTTTCCCACT TCATCGCAGC TTGACTAACA GCTACCCCGC TTGAGCAGAC ATCACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG AGCAGGACTG ATTCCGGATC CACTTAACGT TACTGAAATC ATCAAACAGC TTGACGAATC TGGATATAAG ATCGTTGGTG TCGATGTCAG CTCCGGAGTT GAGACAAATG GTGTTCAGGA TCTCGATAAG ATACGTTCAT TTGTCCAAGC AGCAAAGAGT GCCTTCTAGT GATTTAATAG CTCCATGTCA ACAAGAATAA AACGCGTTTC GGGTTTACCT CTTCCAGATA CAGCTCATCT GCAATGCATT AATGCATTGG ACCTCGCAAC CCTAGTACGC CCTTCAGGCT CCGGCGAAGC AGAAGAATAG CTTAGCAGAG TCTATTTTCA TTTTCGGGAG ACGAGATCAA GCAGATCAAC GGTCGTCAAG AGACCTACGA GACTGAGGAA TCCGCTCTTG GCTCCACGCG ACTATATATT TGTCTCTAAT TGTACTTTGA CATGCTCCTC TTCTTTACTC TGATAGCTTG ACTATGAAAA TTCCGTCACC AGCCCCTGGG TTCGCAAAGA TAATTGCACT GTTTCTTCCT TGAACTCTCA AGCCTACAGG ACACACATTC ATCGTAGGTA TAAACCTCGA AAATCATTCC TACTAAGATG GGTATACAAT AGTAACCATG CATGGTTGCC TAGTGAATGC TCCGTAACAC CCAATACGCC GGCCGAAACT TTTTTACAAC TCTCCTATGA GTCGTTTACC CAGAATGCAC AGGTACACTT GTTTAGAGGT AATCCTTCTT TCTAGAAGTC CTCGTGTACT GTGTAAGCGC CCACTCCACA TCTCCACTCG ACCTGCAGGC ATGCAAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTC
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