pBE2R-EGFP
$84.00
In stock
SKU
PL81928
100μg liquid plasmid(Instantly):$120
| name | pBE2R-EGFP | another name | apBE2R-EGFP |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression,Secretion expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Kan | Fluorescent labeling | Green |
| Promoter | P43 | Replicon | repB |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTATTTAGTT ATTTGTTTAG TCACTTATAA CTTTAACAGA TGGGGTTTTT CTGTGCAACC AATTTTAAGG GTTTTCAATA CTTTAAAACA CATACATACC AACACTTCAA CGCACCTTTC AGCAACTAAA ATAAAAATGA CGTTATTTCT ATATGTATCA AGATAAGAAA GAACAAGTTC AAAACCATCA AAAAAAGACA CCTTTTCAGG TGCTTTTTTT ATTTTATAAA CTCATTCCCT GATCTCGACT TCGTTCTTTT TTTACCTCTC GGTTATGAGT TAGTTCAAAT TCGTTCTTTT TAGGTTCTAA ATCGTGTTTT TCTTGGAATT GTGCTGTTTT ATCCTTTACC TTGTCTACAA ACCCCTTAAA AACGTTTTTA AAGGCTTTTA AGCCGTCTGT ACGTTCCTTA AGGAATTCGA GCTCAGCTTT ATTGAGTGGA TGATTATATT CCTTTTGATA GGTGGTATGT TTTCGCTTGA ACTTTTAAAT ACAGCCATTG AACATACGGT TGATTTAATA ACTGACAAAC ATCACCCTCT TGCTAAAGCG GCCAAGGACG CTGCCGCCGG GGCTGTTTGC GTTTTTGCCG TGATTTCGTG TATTATTGGT TTACTTATTT TTTTGCCAAA GCTGTAATGG CTGAAAATTC TTACATTTAT TTTACATTTT TAGAAATGGG CGTGAAAAAA AGCGCGCGAT TATGTAAAAT ATAAAGTGAT AGCGGTACCA TTATAGGTAA GAGAGGAATG TACACATGAA GAAACCGTTG GGGAAAATTG TCGCAAGCAC CGCACTACTC ATTTCTGTTG CTTTTAGTTC ATCGATCGCA TCGGCTGCTG AAGAAGCAAA AGAAAAATAT TTAATTGGCT TTAATGAGCA GGAAGCTGTC AGTGAGTTTG TAGAACAAGT AGAGGCAAAT GACGAGGTCG CCATTCTCTC TGAGGAAGAG GAAGTCGAAA TTGAATTGCT TCATGAATTT GAAACGATTC CTGTTTTATC CGTTGAGTTA AGCCCAGAAG ATGTGGACGC GCTTGAACTC GATCCAGCGA TTTCTTATAT TGAAGAGGAT GCAGAAGTAA CGACAATGGG ATCCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GCTCGAGCAC CACCACCACC ACCACAAGCT TGTATTCTAT AGTGTCACCT AAATCGTATG TGTATGATAC ATAAGGTTAT GTATTAATTG TAGCCGCGTT CTAACGACAA TATGTACAAG CCTAATTGTG TAGCATCTGG CTTACTGAAG CAGACCCTAT CATCTCTCTC GTAAACTGCC GTCAGAGTCG GTTTGGTTGG ACGAACCTTC TGAGTTTCTG GTAACGCCGT CCCGCACCCG GAAATGGTCA GCGAACCAAT CAGCAGGGTC ATCGCTAGCC AGATCCTCTA CGCCGGACGC ATCGTGGCCG GCATCACCGG CGCCACAGGT GCGGTTGCTG GCGCCTATAT CGCCGACATC ACCGATGGGG AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT CGGCGTGGGT ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA GCGTCGAATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GATTTCACTT TTTGCATTCT ACAAACTGCA TAACTCATAT GTAAATCGCT CCTTTTTAGG TGGCACAAAT GTGAGGCATT TTCGCTCTTT CCGGCAACCA CTTCCAAGTA AAGTATAACA CACTATACTT TATATTCATA AAGTGTGTGC TCTGCGAGGC TGTCGGCAGT GCCGACCAAA ACCATAAAAC CTTTAAGACC TTTCTTTTTT TTACGAGAAA AAAGAAACAA AAAAACCTGC CCTCTGCCAC CTCAGCAAAG GGGGGTTTTG CTCTCGTGCT CGTTTAAAAA TCAGCAAGGG ACAGGTAGTA TTTTTTGAGA AGATCACTCA AAAAATCTCC ACCTTTAAAC CCTTGCCAAT TTTTATTTTG TCCGTTTTGT CTAGCTTACC GAAAGCCAGA CTCAGCAAGA ATAAAATTTT TATTGTCTTT CGGTTTTCTA GTGTAACGGA CAAAACCACT CAAAATAAAA AAGATACAAG AGAGGTCTCT CGTATCTTTT ATTCAGCAAT CGCGCCCGAT TGCTGAACAG ATTAATAATA GATTTTAGCT TTTTATTTGT TGAAAAAAGC TAATCAAATT GTTGTCGGGA TCAATTACTG CAAAGTCTCG TTCATCCCAC CACTGATCTT TTAATGATGT ATTGGGGTGC AAAATGCCCA AAGGCTTAAT ATGTTGATAT AATTCATCAA TTCCCTCTAC TTCAATGCGG CAACTAGCAG TACCAGCAAT AAACGACTCC GCACCTGTAC AAACCGGTGA ATCATTACTA CGAGAGCGCC AGCTTCATCA CTTGCCTCCC ATAGATGAAT CCGAACCTCA TTACACATTA GAACTGCGAA TCCATCTTCA TGGTGAACCA AAGTGAAACC TAGTTTATCG CAATAAAAAC CTATACTCTT TTTAATATCC CCGACTGGCA ATGCCGGGAT AGACTGTAAC ATTCTCACGC ATAAAATCCC CTTTCATTTT CTAATGTAAA TCTATTACCT TATTATTAAT TCAATTCGCT CATAATTAAT CCTTTTTCTT ATTACGCAAA ATGGCCCGAT TTAAGCACAC CCTTTATTCC GTTAATGCGC CATGACAGCC ATGATAATTA CTAATACTAG GAGAAGTTAA TAAATACGTA ACCAACATGA TTAACAATTA TTAGAGGTCA TCGTTCAAAA TGGTATGCGT TTTGACACAT CCACTATATA TCCGTGTCGT TCTGTCCACT CCTGAATCCC ATTCCAGAAA TTCTCTAGCG ATTCCAGAAG TTTCTCAGAG TCGGAAAGTT GACCAGACAT TACGAACTGG CACAGATGGT CATAACCTGA AGGAAGATCT GATTGCTTAA CTGCTTCAGT TAAGACCGAA GCGCTCGTCG TATAACAGAT GCGATGATGC AGACCAATCA ACATGGCACC TGCCATTGCT ACCTGTACAG TCAAGGATGG TAGAAATGTT GTCGGTCCTT GCACACGAAT ATTACGCCAT TTGCCTGCAT ATTCAAACAG CTCTTCTACG ATAAGGGCAC AAATCGCATC GTGGAACGTT TGGGCTTCTA CCGATTTAGC AGTTTGATAC ACTTTCTCTA AGTATCCACC TGAATCATAA ATCGGCAAAA TAGAGAAAAA TTGACCATGT GTAAGCGGCC AATCTGATTC CACCTGAGAT GCATAATCTA GTAGAATCTC TTCGCTATCA AAATTCACTT CCACCTTCCA CTCACCGGTT GTCCATTCAT GGCTGAACTC TGCTTCCTCT GTTGACATGA CACACATCAT CTCAATATCC GAATAGGGCC CATCAGTCTG ACGACCAAGA GAGCCATAAA CACCAATAGC CTTAACATCA TCCCCATATT TATCCAATAT TCGTTCCTTA ATTTCATGAA CAATCTTCAT TCTTTCTTCT CTAGTCATTA TTATTGGTCC ATTCACTATT CTCATTCCCT TTTCAGATAA TTTTAGATTT GCTTTTCTAA ATAAGAATAT TTGGAGAGCA CCGTTCTTAT TCAGCTATTA ATAACTCGTC TTCCTAAGCA TCCTTCAATC CTTTTAATAA CAATTATAGC ATCTAATCTT CAACAAACTG GCCCGTTTGT TGAACTACTC TTTAATAAAA TAATTTTTCC GTTCCCAATT CCACATTGCA ATAATAGAAA ATCCATCTTC ATCGGCTTTT TCGTCATCAT CTGTATGAAT CAAATCGCCT TCTTCTGTGT CATCAAGGTT TAATTTTTTA TGTATTTCTT TTAACAAACC ACCATAGGAG ATTAACCTTT TACGGTGTAA ACCTTCCTCC AAATCAGACA AACGTTTCAA ATTCTTTTCT TCATCATCGG TCATAAAATC CGTATCCTTT ACAGGATATT TTGCAGTTTC GTCAATTGCC GATTGTATAT CCGATTTATA TTTATTTTTC GGTCGAATCA TTTGAACTTT TACATTTGGA TCATAGTCTA ATTTCATTGC CTTTTTCCAA AATTGAATCC ATTGTTTTTG ATTCACGTAG TTTTCTGTAT TCTTAAAATA AGTTGGTTCC ACACATACCA ATACATGCAT GTGCTGATTA TAAGAATTAT CTTTATTATT TATTGTCACT TCCGTTGCAC GCATAAAACC AACAAGATTT TTATTAATTT TTTTATATTG CATCATTCGG CGAAATCCTT GAGCCATATC TGACAAACTC TTATTTAATT CTTCGCCATC ATAAACATTT TTAACTGTTA ATGTGAGAAA CAACCAACGA ACTGTTGGCT TTTGTTTAAT AACTTCAGCA ACAACCTTTT GTGACTGAAT GCCATGTTTC ATTGCTCTCC TCCAGTTGCA CATTGGACAA AGCCTGGATT TACAAAACCA CACTCGATAC AACTTTCTTT CGCCTGTTTC ACGATTTTGT TTATACTCTA ATATTTCAGC ACAATCTTTT ACTCTTTCAG CCTTTTTAAA TTCAAGAATA TGCAGAAGTT CAAAGTAATC AACATTAGCG ATTTTCTTTT CTCTCCATGG TCTCACTTTT CCACTTTTTG TCTTGTCCAC TAAAACCCTT GATTTTTCAT CTGAATAAAT GCTACTATTA GGACACATAA TATTAAAAGA AACCCCCAT
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