pSH47
$99.00
In stock
SKU
PL81913
100μg liquid plasmid(Instantly):$120
| name | pSH47 | another name | apSH47 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Gene editing | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATACC ACAGCTTTTC AATTCAATTC ATCATTTTTT TTTTATTCTT TTTTTTGATT TCGGTTTCTT TGAAATTTTT TTGATTCGGT AATCTCCGAA CAGAAGGAAG AACGAAGGAA GGAGCACAGA CTTAGATTGG TATATATACG CATATGTAGT GTTGAAGAAA CATGAAATTG CCCAGTATTC TTAACCCAAC TGCACAGAAC AAAAACCTGC AGGAAACGAA GATAAATCAT GTCGAAAGCT ACATATAAGG AACGTGCTGC TACTCATCCT AGTCCTGTTG CTGCCAAGCT ATTTAATATC ATGCACGAAA AGCAAACAAA CTTGTGTGCT TCATTGGATG TTCGTACCAC CAAGGAATTA CTGGAGTTAG TTGAAGCATT AGGTCCCAAA ATTTGTTTAC TAAAAACACA TGTGGATATC TTGACTGATT TTTCCATGGA GGGCACAGTT AAGCCGCTAA AGGCATTATC CGCCAAGTAC AATTTTTTAC TCTTCGAAGA CAGAAAATTT GCTGACATTG GTAATACAGT CAAATTGCAG TACTCTGCGG GTGTATACAG AATAGCAGAA TGGGCAGACA TTACGAATGC ACACGGTGTG GTGGGCCCAG GTATTGTTAG CGGTTTGAAG CAGGCGGCAG AAGAAGTAAC AAAGGAACCT AGAGGCCTTT TGATGTTAGC AGAATTGTCA TGCAAGGGCT CCCTATCTAC TGGAGAATAT ACTAAGGGTA CTGTTGACAT TGCGAAGAGC GACAAAGATT TTGTTATCGG CTTTATTGCT CAAAGAGACA TGGGTGGAAG AGATGAAGGT TACGATTGGT TGATTATGAC ACCCGGTGTG GGTTTAGATG ACAAGGGAGA CGCATTGGGT CAACAGTATA GAACCGTGGA TGATGTGGTC TCTACAGGAT CTGACATTAT TATTGTTGGA AGAGGACTAT TTGCAAAGGG AAGGGATGCT AAGGTAGAGG GTGAACGTTA CAGAAAAGCA GGCTGGGAAG CATATTTGAG AAGATGCGGC CAGCAAAACT AAAAAACTGT ATTATAAGTA AATGCATGTA TACTAAACTC ACAAATTAGA GCTTCAATTT AATTATATCA GTTATTACCC TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGCCGCAAA TTAAAGCCTT CGAGCGTCCC AAAACCTTCT CAAGCAAGGT TTTCAGTATA ATGTTACATG CGTACACGCG TCTGTACAGA AAAAAAAGAA AAATTTGAAA TATAAATAAC GTTCTTAATA CTAACATAAC TATAAAAAAA TAAATAGGGA CCTAGACTTC AGGTTGTCTA ACTCCTTCCT TTTCGGTTAG AGCGGATGTG GGGGGAGGGC GTGAATGTAA GCGTGACATA ACTAATTACA TGACTCGAGG TCGACAATTC CAACCTTACC CAAGAGTTCG CCAAACTCAG ACATCACTTT AGCAAAACCG CGCCGTGCTT CTTCCTCGGT GGCATTCATC ACGAAATGTT CAGCACTACG CATACTTTTG ACAGGAAACG CAACGGATAT TGAGTCAATA TCAGGCATTC TATCGCTCAG CTTTACAGTG ACAATGACGG CTGGCGACTG AATATTAGTG CTTACAGACA GCACTACATA TTTTCCGTCG ATGTTGAAAT CCTTTCTCAT ATGTCACCAT AAATATCAAA TAATTATAGC AATCATTTAC GCGTTAATGG CTAATCGCCA TCTTCCAGCA GGCGCACCAT TGCCCCTGTT TCACTATCCA GGGTACGGAT ATAGTTCATG ACAATATTTA CATTGGTCCA GCCACCAGCT TGCATGATCT CCGGTATTGA AACTCCAGCG CGGGCCATAT CTCGCGCGGC TCCGACACGG GCACTGTGTC CAGACCAGGC CAGGTATCTC TGACCAGAGT CATCCTTAGC GCCGTAAATC AATCGATGAG TTGCTTCAAA AATCCCTTCC AGGGCGCGAG TTGATAGCTG GCTGGTGGCA GATGGCGCGG CAACACCATT TTTTCTGACC CGGCAAAACA GGTAGTTATT CGGATCATCA GCTACACCAG AGACGGAAAT CCATCGCTCG ACCAGTTTAG TTACCCCCAG GCTAAGTGCC TTCTCTACAC CTGCGGTGCT AACCAGCGTT TTCGTTCTGC CAATATGGAT TAACATTCTC CCACCGTCAG TACGTGAGAT ATCTTTAACC CTGATCCTGG CAATTTCGGC TATACGTAAC AGGGTGTTAT AAGCAATCCC CAGAAATGCC AGATTACGTA TATCCTGGCA GCGATCGCTA TTTTCCATGA GTGAACGAAC CTGGTCGAAA TCAGTGCGTT CGAACGCTAG AGCCTGTTTT GCACGTTCAC CGGCATCAAC GTTTTCTTTT CGGATCCGCC GCATAACCAG TGAAACAGCA TTGCTGTCAC TTGGTCGTGG CAGCCCGGAC CGACGATGAA GCATGTTTAG CTGGCCCAAA TGTTGCTGGA TAGTTTTTAC TGCCAGACCG CGCGCCTGAA GATATAGAAG ATAATCGCGA ACATCTTCAG GTTCTGCGGG AAACCATTTC CGGTTATTCA ACTTGCACCA TGCCGCCCAC GACCGGCAAA CGGACAGAAG CATTTTCCAG GTATGCTCAG AAAACGCCTG GCGATCCCTG AACATGTCCA TCAGGTTCTT GCGAACCTCA TCACTCGTTG CATCGACCGG TAATGCAGGC AAATTTTGGT GTACGGTCAG TAAATTGGAC ATTTAACACT CAGATAATGG TTTTAAGTAA AGTGTACAGG ATCGGCTCTG CCCCTCGACG GTATCGATAA GCTTGATATC GAATTCCTGC AGCCCGGGGG ATCCACTAGT TCTAGAATCC GGGGTTTTTT CTCCTTGACG TTAAAGTATA GAGGTATATT AACAATTTTT TGTTGATACT TTTATTACAT TTGAATAAGA AGTAATACAA ACCGAAAATG TTGAAAGTAT TAGTTAAAGT GGTTATGCAG TTTTTGCATT TATATATCTG TTAATAGATC AAAAATCATC GCTTCGCTGA TTAATTACCC CAGAAATAAG GCTAAAAAAC TAATCGCATT ATCATCCTAT GGTTGTTAAT TTGATTCGTT CATTTGAAGG TTTGTGGGGC CAGGTTACTG CCAATTTTTC CTCTTCATAA CCATAAAAGC TAGTATTGTA GAATCTTTAT TGTTCGGAGC AGTGCGGCGC GAGGCACATC TGCGTTTCAG GAACGCGACC GGTGAAGACG AGGACGCACG GAGGAGAGTC TTCCTTCGGA GGGCTGTCAC CCGCTCGGCG GCTTCTAATC CGTACTAGAG CTCCAGCTTT TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAG GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGGTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGGGT CCTTTTCATC ACGTGCTATA AAAATAATTA TAATTTAAAT TTTTTAATAT AAATATATAA ATTAAAAATA GAAAGTAAAA AAAGAAATTA AAGAAAAAAT AGTTTTTGTT TTCCGAAGAT GTAAAAGACT CTAGGGGGAT CGCCAACAAA TACTACCTTT TATCTTGCTC TTCCTGCTCT CAGGTATTAA TGCCGAATTG TTTCATCTTG TCTGTGTAGA AGACCACACA CGAAAATCCT GTGATTTTAC ATTTTACTTA TCGTTAATCG AATGTATATC TATTTAATCT GCTTTTCTTG TCTAATAAAT ATATATGTAA AGTACGCTTT TTGTTGAAAT TTTTTAAACC TTTGTTTATT TTTTTTTCTT CATTCCGTAA CTCTTCTACC TTCTTTATTT ACTTTCTAAA ATCCAAATAC AAAACATAAA AATAAATAAA CACAGAGTAA ATTCCCAAAT TATTCCATCA TTAAAAGATA CGAGGCGCGT GTAAGTTACA GGCAAGCGAT CCGTCCTA
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