pCAMBIA1305.1
$128.00
In stock
SKU
PL81911
100μg liquid plasmid(Instantly):$163
| name | pCAMBIA1305.1 | another name | apCAMBIA1305.1 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Hyg | Fluorescent labeling | Gus |
| Promoter | Replicon | pVS1 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CATGGTAGAT CTGAGGGTAA ATTTCTAGTT TTTCTCCTTC ATTTTCTTGG TTAGGACCCT TTTCTCTTTT TATTTTTTTG AGCTTTGATC TTTCTTTAAA CTGATCTATT TTTTAATTGA TTGGTTATGG TGTAAATATT ACATAGCTTT AACTGATAAT CTGATTACTT TATTTCGTGT GTCTATGATG ATGATGATAG TTACAGAACC GACGAACTAG TCTGTACCCG ATCAACACCG AGACCCGTGG CGTCTTCGAC CTCAATGGCG TCTGGAACTT CAAGCTGGAC TACGGGAAAG GACTGGAAGA GAAGTGGTAC GAAAGCAAGC TGACCGACAC TATTAGTATG GCCGTCCCAA GCAGTTACAA TGACATTGGC GTGACCAAGG AAATCCGCAA CCATATCGGA TATGTCTGGT ACGAACGTGA GTTCACGGTG CCGGCCTATC TGAAGGATCA GCGTATCGTG CTCCGCTTCG GCTCTGCAAC TCACAAAGCA ATTGTCTATG TCAATGGTGA GCTGGTCGTG GAGCACAAGG GCGGATTCCT GCCATTCGAA GCGGAAATCA ACAACTCGCT GCGTGATGGC ATGAATCGCG TCACCGTCGC CGTGGACAAC ATCCTCGACG ATAGCACCCT CCCGGTGGGG CTGTACAGCG AGCGCCACGA AGAGGGCCTC GGAAAAGTCA TTCGTAACAA GCCGAACTTC GACTTCTTCA ACTATGCAGG CCTGCACCGT CCGGTGAAAA TCTACACGAC CCCGTTTACG TACGTCGAGG ACATCTCGGT TGTGACCGAC TTCAATGGCC CAACCGGGAC TGTGACCTAT ACGGTGGACT TTCAAGGCAA AGCCGAGACC GTGAAAGTGT CGGTCGTGGA TGAGGAAGGC AAAGTGGTCG CAAGCACCGA GGGCCTGAGC GGTAACGTGG AGATTCCGAA TGTCATCCTC TGGGAACCAC TGAACACGTA TCTCTACCAG ATCAAAGTGG AACTGGTGAA CGACGGACTG ACCATCGATG TCTATGAAGA GCCGTTCGGC GTGCGGACCG TGGAAGTCAA CGACGGCAAG TTCCTCATCA ACAACAAACC GTTCTACTTC AAGGGCTTTG GCAAACATGA GGACACTCCT ATCAACGGCC GTGGCTTTAA CGAAGCGAGC AATGTGATGG ATTTCAATAT CCTCAAATGG ATCGGCGCCA ACAGCTTCCG GACCGCACAC TATCCGTACT CTGAAGAGTT GATGCGTCTT GCGGATCGCG AGGGTCTGGT CGTGATCGAC GAGACTCCGG CAGTTGGCGT GCACCTCAAC TTCATGGCCA CCACGGGACT CGGCGAAGGC AGCGAGCGCG TCAGTACCTG GGAGAAGATT CGGACGTTTG AGCACCATCA AGACGTTCTC CGTGAACTGG TGTCTCGTGA CAAGAACCAT CCAAGCGTCG TGATGTGGAG CATCGCCAAC GAGGCGGCGA CTGAGGAAGA GGGCGCGTAC GAGTACTTCA AGCCGTTGGT GGAGCTGACC AAGGAACTCG ACCCACAGAA GCGTCCGGTC ACGATCGTGC TGTTTGTGAT GGCTACCCCG GAGACGGACA AAGTCGCCGA ACTGATTGAC GTCATCGCGC TCAATCGCTA TAACGGATGG TACTTCGATG GCGGTGATCT CGAAGCGGCC AAAGTCCATC TCCGCCAGGA ATTTCACGCG TGGAACAAGC GTTGCCCAGG AAAGCCGATC ATGATCACTG AGTACGGCGC AGACACCGTT GCGGGCTTTC ACGACATTGA TCCAGTGATG TTCACCGAGG AATATCAAGT CGAGTACTAC CAGGCGAACC ACGTCGTGTT CGATGAGTTT GAGAACTTCG TGGGTGAGCA AGCGTGGAAC TTCGCGGACT TCGCGACCTC TCAGGGCGTG ATGCGCGTCC AAGGAAACAA GAAGGGCGTG TTCACTCGTG ACCGCAAGCC GAAGCTCGCC GCGCACGTCT TTCGCGAGCG CTGGACCAAC ATTCCAGATT TCGGCTACAA GAACGCTAGC CATCACCATC ACCATCACGT GTGAATTGGT GACCAGCTCG AATTTCCCCG ATCGTTCAAA CATTTGGCAA TAAAGTTTCT TAAGATTGAA TCCTGTTGCC GGTCTTGCGA TGATTATCAT ATAATTTCTG TTGAATTACG TTAAGCATGT AATAATTAAC ATGTAATGCA TGACGTTATT TATGAGATGG GTTTTTATGA TTAGAGTCCC GCAATTATAC ATTTAATACG CGATAGAAAA CAAAATATAG CGCGCAAACT AGGATAAATT ATCGCGCGCG GTGTCATCTA TGTTACTAGA TCGGGAATTA AACTATCAGT GTTTGACAGG ATATATTGGC GGGTAAACCT AAGAGAAAAG AGCGTTTATT AGAATAACGG ATATTTAAAA GGGCGTGAAA AGGTTTATCC GTTCGTCCAT TTGTATGTGC ATGCCAACCA CAGGGTTCCC CTCGGGATCA AAGTACTTTG ATCCAACCCC TCCGCTGCTA TAGTGCAGTC GGCTTCTGAC GTTCAGTGCA GCCGTCTTCT GAAAACGACA TGTCGCACAA GTCCTAAGTT ACGCGACAGG CTGCCGCCCT GCCCTTTTCC TGGCGTTTTC TTGTCGCGTG TTTTAGTCGC ATAAAGTAGA ATACTTGCGA CTAGAACCGG AGACATTACG CCATGAACAA GAGCGCCGCC GCTGGCCTGC TGGGCTATGC CCGCGTCAGC ACCGACGACC AGGACTTGAC CAACCAACGG GCCGAACTGC ACGCGGCCGG CTGCACCAAG CTGTTTTCCG AGAAGATCAC CGGCACCAGG CGCGACCGCC CGGAGCTGGC CAGGATGCTT GACCACCTAC GCCCTGGCGA CGTTGTGACA GTGACCAGGC TAGACCGCCT GGCCCGCAGC ACCCGCGACC TACTGGACAT TGCCGAGCGC ATCCAGGAGG CCGGCGCGGG CCTGCGTAGC CTGGCAGAGC CGTGGGCCGA CACCACCACG CCGGCCGGCC GCATGGTGTT GACCGTGTTC GCCGGCATTG CCGAGTTCGA GCGTTCCCTA ATCATCGACC GCACCCGGAG CGGGCGCGAG GCCGCCAAGG CCCGAGGCGT GAAGTTTGGC CCCCGCCCTA CCCTCACCCC GGCACAGATC GCGCACGCCC GCGAGCTGAT CGACCAGGAA GGCCGCACCG TGAAAGAGGC GGCTGCACTG CTTGGCGTGC ATCGCTCGAC CCTGTACCGC GCACTTGAGC GCAGCGAGGA AGTGACGCCC ACCGAGGCCA GGCGGCGCGG TGCCTTCCGT GAGGACGCAT TGACCGAGGC CGACGCCCTG GCGGCCGCCG AGAATGAACG CCAAGAGGAA CAAGCATGAA ACCGCACCAG GACGGCCAGG ACGAACCGTT TTTCATTACC GAAGAGATCG AGGCGGAGAT GATCGCGGCC GGGTACGTGT TCGAGCCGCC CGCGCACGTC TCAACCGTGC GGCTGCATGA AATCCTGGCC GGTTTGTCTG ATGCCAAGCT GGCGGCCTGG CCGGCCAGCT TGGCCGCTGA AGAAACCGAG CGCCGCCGTC TAAAAAGGTG ATGTGTATTT GAGTAAAACA GCTTGCGTCA TGCGGTCGCT GCGTATATGA TGCGATGAGT AAATAAACAA ATACGCAAGG GGAACGCATG AAGGTTATCG CTGTACTTAA CCAGAAAGGC GGGTCAGGCA AGACGACCAT CGCAACCCAT CTAGCCCGCG CCCTGCAACT CGCCGGGGCC GATGTTCTGT TAGTCGATTC CGATCCCCAG GGCAGTGCCC GCGATTGGGC GGCCGTGCGG GAAGATCAAC CGCTAACCGT TGTCGGCATC GACCGCCCGA CGATTGACCG CGACGTGAAG GCCATCGGCC GGCGCGACTT CGTAGTGATC GACGGAGCGC CCCAGGCGGC GGACTTGGCT GTGTCCGCGA TCAAGGCAGC CGACTTCGTG CTGATTCCGG TGCAGCCAAG CCCTTACGAC ATATGGGCCA CCGCCGACCT GGTGGAGCTG GTTAAGCAGC GCATTGAGGT CACGGATGGA AGGCTACAAG CGGCCTTTGT CGTGTCGCGG GCGATCAAAG GCACGCGCAT CGGCGGTGAG GTTGCCGAGG CGCTGGCCGG GTACGAGCTG CCCATTCTTG AGTCCCGTAT CACGCAGCGC GTGAGCTACC CAGGCACTGC CGCCGCCGGC ACAACCGTTC TTGAATCAGA ACCCGAGGGC GACGCTGCCC GCGAGGTCCA GGCGCTGGCC GCTGAAATTA AATCAAAACT CATTTGAGTT AATGAGGTAA AGAGAAAATG AGCAAAAGCA CAAACACGCT AAGTGCCGGC CGTCCGAGCG CACGCAGCAG CAAGGCTGCA ACGTTGGCCA GCCTGGCAGA CACGCCAGCC ATGAAGCGGG TCAACTTTCA GTTGCCGGCG GAGGATCACA CCAAGCTGAA GATGTACGCG GTACGCCAAG GCAAGACCAT TACCGAGCTG CTATCTGAAT ACATCGCGCA GCTACCAGAG TAAATGAGCA AATGAATAAA TGAGTAGATG AATTTTAGCG GCTAAAGGAG GCGGCATGGA AAATCAAGAA CAACCAGGCA CCGACGCCGT GGAATGCCCC ATGTGTGGAG GAACGGGCGG TTGGCCAGGC GTAAGCGGCT GGGTTGTCTG CCGGCCCTGC AATGGCACTG GAACCCCCAA GCCCGAGGAA TCGGCGTGAG CGGTCGCAAA CCATCCGGCC CGGTACAAAT CGGCGCGGCG CTGGGTGATG ACCTGGTGGA GAAGTTGAAG GCCGCGCAGG CCGCCCAGCG GCAACGCATC GAGGCAGAAG CACGCCCCGG TGAATCGTGG CAAGCGGCCG CTGATCGAAT CCGCAAAGAA TCCCGGCAAC CGCCGGCAGC CGGTGCGCCG TCGATTAGGA AGCCGCCCAA GGGCGACGAG CAACCAGATT TTTTCGTTCC GATGCTCTAT GACGTGGGCA CCCGCGATAG TCGCAGCATC ATGGACGTGG CCGTTTTCCG TCTGTCGAAG CGTGACCGAC GAGCTGGCGA GGTGATCCGC TACGAGCTTC CAGACGGGCA CGTAGAGGTT TCCGCAGGGC CGGCCGGCAT GGCCAGTGTG TGGGATTACG ACCTGGTACT GATGGCGGTT TCCCATCTAA CCGAATCCAT GAACCGATAC CGGGAAGGGA AGGGAGACAA GCCCGGCCGC GTGTTCCGTC CACACGTTGC GGACGTACTC AAGTTCTGCC GGCGAGCCGA TGGCGGAAAG CAGAAAGACG ACCTGGTAGA AACCTGCATT CGGTTAAACA CCACGCACGT TGCCATGCAG CGTACGAAGA AGGCCAAGAA CGGCCGCCTG GTGACGGTAT CCGAGGGTGA AGCCTTGATT AGCCGCTACA AGATCGTAAA GAGCGAAACC GGGCGGCCGG AGTACATCGA GATCGAGCTA GCTGATTGGA TGTACCGCGA GATCACAGAA GGCAAGAACC CGGACGTGCT GACGGTTCAC CCCGATTACT TTTTGATCGA TCCCGGCATC GGCCGTTTTC TCTACCGCCT GGCACGCCGC GCCGCAGGCA AGGCAGAAGC CAGATGGTTG TTCAAGACGA TCTACGAACG CAGTGGCAGC GCCGGAGAGT TCAAGAAGTT CTGTTTCACC GTGCGCAAGC TGATCGGGTC AAATGACCTG CCGGAGTACG ATTTGAAGGA GGAGGCGGGG CAGGCTGGCC CGATCCTAGT CATGCGCTAC CGCAACCTGA TCGAGGGCGA AGCATCCGCC GGTTCCTAAT GTACGGAGCA GATGCTAGGG CAAATTGCCC TAGCAGGGGA AAAAGGTCGA AAAGGTCTCT TTCCTGTGGA TAGCACGTAC ATTGGGAACC CAAAGCCGTA CATTGGGAAC CGGAACCCGT ACATTGGGAA CCCAAAGCCG TACATTGGGA ACCGGTCACA CATGTAAGTG ACTGATATAA AAGAGAAAAA AGGCGATTTT TCCGCCTAAA ACTCTTTAAA ACTTATTAAA ACTCTTAAAA CCCGCCTGGC CTGTGCATAA CTGTCTGGCC AGCGCACAGC CGAAGAGCTG CAAAAAGCGC CTACCCTTCG GTCGCTGCGC TCCCTACGCC CCGCCGCTTC GCGTCGGCCT ATCGCGGCCG CTGGCCGCTC AAAAATGGCT GGCCTACGGC CAGGCAATCT ACCAGGGCGC GGACAAGCCG CGCCGTCGCC ACTCGACCGC CGGCGCCCAC ATCAAGGCAC CCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC ACGTAGCGAT AGCGGAGTGT ATACTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGCATTCTAG GTACTAAAAC AATTCATCCA GTAAAATATA ATATTTTATT TTCTCCCAAT CAGGCTTGAT CCCCAGTAAG TCAAAAAATA GCTCGACATA CTGTTCTTCC CCGATATCCT CCCTGATCGA CCGGACGCAG AAGGCAATGT CATACCACTT GTCCGCCCTG CCGCTTCTCC CAAGATCAAT AAAGCCACTT ACTTTGCCAT CTTTCACAAA GATGTTGCTG TCTCCCAGGT CGCCGTGGGA AAAGACAAGT TCCTCTTCGG GCTTTTCCGT CTTTAAAAAA TCATACAGCT CGCGCGGATC TTTAAATGGA GTGTCTTCTT CCCAGTTTTC GCAATCCACA TCGGCCAGAT CGTTATTCAG TAAGTAATCC AATTCGGCTA AGCGGCTGTC TAAGCTATTC GTATAGGGAC AATCCGATAT GTCGATGGAG TGAAAGAGCC TGATGCACTC CGCATACAGC TCGATAATCT TTTCAGGGCT TTGTTCATCT TCATACTCTT CCGAGCAAAG GACGCCATCG GCCTCACTCA TGAGCAGATT GCTCCAGCCA TCATGCCGTT CAAAGTGCAG GACCTTTGGA ACAGGCAGCT TTCCTTCCAG CCATAGCATC ATGTCCTTTT CCCGTTCCAC ATCATAGGTG GTCCCTTTAT ACCGGCTGTC CGTCATTTTT AAATATAGGT TTTCATTTTC TCCCACCAGC TTATATACCT TAGCAGGAGA CATTCCTTCC GTATCTTTTA CGCAGCGGTA TTTTTCGATC AGTTTTTTCA ATTCCGGTGA TATTCTCATT TTAGCCATTT ATTATTTCCT TCCTCTTTTC TACAGTATTT AAAGATACCC CAAGAAGCTA ATTATAACAA GACGAACTCC AATTCACTGT TCCTTGCATT CTAAAACCTT AAATACCAGA AAACAGCTTT TTCAAAGTTG TTTTCAAAGT TGGCGTATAA CATAGTATCG ACGGAGCCGA TTTTGAAACC GCGGTGATCA CAGGCAGCAA CGCTCTGTCA TCGTTACAAT CAACATGCTA CCCTCCGCGA GATCATCCGT GTTTCAAACC CGGCAGCTTA GTTGCCGTTC TTCCGAATAG CATCGGTAAC ATGAGCAAAG TCTGCCGCCT TACAACGGCT CTCCCGCTGA CGCCGTCCCG GACTGATGGG CTGCCTGTAT CGAGTGGTGA TTTTGTGCCG AGCTGCCGGT CGGGGAGCTG TTGGCTGGCT GGTGGCAGGA TATATTGTGG TGTAAACAAA TTGACGCTTA GACAACTTAA TAACACATTG CGGACGTTTT TAATGTACTG AATTAACGCC GAATTAATTC GGGGGATCTG GATTTTAGTA CTGGATTTTG GTTTTAGGAA TTAGAAATTT TATTGATAGA AGTATTTTAC AAATACAAAT ACATACTAAG GGTTTCTTAT ATGCTCAACA CATGAGCGAA ACCCTATAGG AACCCTAATT CCCTTATCTG GGAACTACTC ACACATTATT ATGGAGAAAC TCGAGCTTGT CGATCGACAG ATCCGGTCGG CATCTACTCT ATTTCTTTGC CCTCGGACGA GTGCTGGGGC GTCGGTTTCC ACTATCGGCG AGTACTTCTA CACAGCCATC GGTCCAGACG GCCGCGCTTC TGCGGGCGAT TTGTGTACGC CCGACAGTCC CGGCTCCGGA TCGGACGATT GCGTCGCATC GACCCTGCGC CCAAGCTGCA TCATCGAAAT TGCCGTCAAC CAAGCTCTGA TAGAGTTGGT CAAGACCAAT GCGGAGCATA TACGCCCGGA GTCGTGGCGA TCCTGCAAGC TCCGGATGCC TCCGCTCGAA GTAGCGCGTC TGCTGCTCCA TACAAGCCAA CCACGGCCTC CAGAAGAAGA TGTTGGCGAC CTCGTATTGG GAATCCCCGA ACATCGCCTC GCTCCAGTCA ATGACCGCTG TTATGCGGCC ATTGTCCGTC AGGACATTGT TGGAGCCGAA ATCCGCGTGC ACGAGGTGCC GGACTTCGGG GCAGTCCTCG GCCCAAAGCA TCAGCTCATC GAGAGCCTGC GCGACGGACG CACTGACGGT GTCGTCCATC ACAGTTTGCC AGTGATACAC ATGGGGATCA GCAATCGCGC ATATGAAATC ACGCCATGTA GTGTATTGAC CGATTCCTTG CGGTCCGAAT GGGCCGAACC CGCTCGTCTG GCTAAGATCG GCCGCAGCGA TCGCATCCAT AGCCTCCGCG ACCGGTTGTA GAACAGCGGG CAGTTCGGTT TCAGGCAGGT CTTGCAACGT GACACCCTGT GCACGGCGGG AGATGCAATA GGTCAGGCTC TCGCTAAACT CCCCAATGTC AAGCACTTCC GGAATCGGGA GCGCGGCCGA TGCAAAGTGC CGATAAACAT AACGATCTTT GTAGAAACCA TCGGCGCAGC TATTTACCCG CAGGACATAT CCACGCCCTC CTACATCGAA GCTGAAAGCA CGAGATTCTT CGCCCTCCGA GAGCTGCATC AGGTCGGAGA CGCTGTCGAA CTTTTCGATC AGAAACTTCT CGACAGACGT CGCGGTGAGT TCAGGCTTTT TCATATCTCA TTGCCCCCCG GGATCTGCGA AAGCTCGAGA GAGATAGATT TGTAGAGAGA GACTGGTGAT TTCAGCGTGT CCTCTCCAAA TGAAATGAAC TTCCTTATAT AGAGGAAGGT CTTGCGAAGG ATAGTGGGAT TGTGCGTCAT CCCTTACGTC AGTGGAGATA TCACATCAAT CCACTTGCTT TGAAGACGTG GTTGGAACGT CTTCTTTTTC CACGATGCTC CTCGTGGGTG GGGGTCCATC TTTGGGACCA CTGTCGGCAG AGGCATCTTG AACGATAGCC TTTCCTTTAT CGCAATGATG GCATTTGTAG GTGCCACCTT CCTTTTCTAC TGTCCTTTTG ATGAAGTGAC AGATAGCTGG GCAATGGAAT CCGAGGAGGT TTCCCGATAT TACCCTTTGT TGAAAAGTCT CAATAGCCCT TTGGTCTTCT GAGACTGTAT CTTTGATATT CTTGGAGTAG ACGAGAGTGT CGTGCTCCAC CATGTTATCA CATCAATCCA CTTGCTTTGA AGACGTGGTT GGAACGTCTT CTTTTTCCAC GATGCTCCTC GTGGGTGGGG GTCCATCTTT GGGACCACTG TCGGCAGAGG CATCTTGAAC GATAGCCTTT CCTTTATCGC AATGATGGCA TTTGTAGGTG CCACCTTCCT TTTCTACTGT CCTTTTGATG AAGTGACAGA TAGCTGGGCA ATGGAATCCG AGGAGGTTTC CCGATATTAC CCTTTGTTGA AAAGTCTCAA TAGCCCTTTG GTCTTCTGAG ACTGTATCTT TGATATTCTT GGAGTAGACG AGAGTGTCGT GCTCCACCAT GTTGGCAAGC TGCTCTAGCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGAATTCG AGCTCGGTAC CCGGGGATCC TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT GGCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGC TAGAGCAGCT TGAGCTTGGA TCAGATTGTC GTTTCCCGCC TTCAGTTTAG CTTCATGGAG TCAAAGATTC AAATAGAGGA CCTAACAGAA CTCGCCGTAA AGACTGGCGA ACAGTTCATA CAGAGTCTCT TACGACTCAA TGACAAGAAG AAAATCTTCG TCAACATGGT GGAGCACGAC ACACTTGTCT ACTCCAAAAA TATCAAAGAT ACAGTCTCAG AAGACCAAAG GGCAATTGAG ACTTTTCAAC AAAGGGTAAT ATCCGGAAAC CTCCTCGGAT TCCATTGCCC AGCTATCTGT CACTTTATTG TGAAGATAGT GGAAAAGGAA GGTGGCTCCT ACAAATGCCA TCATTGCGAT AAAGGAAAGG CCATCGTTGA AGATGCCTCT GCCGACAGTG GTCCCAAAGA TGGACCCCCA CCCACGAGGA GCATCGTGGA AAAAGAAGAC GTTCCAACCA CGTCTTCAAA GCAAGTGGAT TGATGTGATA TCTCCACTGA CGTAAGGGAT GACGCACAAT CCCACTATCC TTCGCAAGAC CCTTCCTCTA TATAAGGAAG TTCATTTCAT TTGGAGAGAA CACGGGGGAC TCTTGAC
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