pDawn-mCherry
$99.00
In stock
SKU
PL81835
100μg liquid plasmid(Instantly):$120
| name | pDawn-mCherry | another name | apDawn-mCherry |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT CAATTGTCAA TCGTTGAGCA TGCCGGCGCG CATCGCGAGG CGAACCAGCT CCGAAAGGCT GTTGGCCTGC ATCTTGGTCA TGACGTTGGC CCGATACACC TCGATGGTGC GCGGGCTGAT GTCGTACTCG CGGGCGATCA GCTTGTTGGA AAGGCCGGCG ATCAGCCCTT CCATGACCTG GCGCTCCCTG GGGCTCAACG AGGCGACGCG GGCGGCGATA TCCTGCGCGA CGGCCTCGCT CTTGGCGGCC GGCTCGGCCT GGCGGATCGC CGATTCGATC ATGGCGGTGA GGCGGTCGTC CTCGAAAGGC TTTTCCAGAA AGTCCACCGC CCCTAACTTC ATCGCCTCGA CCGCGAGCGG CACGTCGCCG TGACCGGTCA TGATGAGGAT CGGAAAGGGG CTTTGCTGCG CCTTCATCCG CTTCAACAGC TCGATGCCGT CAAGGCCCGG CATGCGCACG TCGGAGACGA CGCAGCCGAA GGAGAGACCC GGCAGGGCGT CGAGAAAGGC TTGCGCGTCG TCAAACAGCG TGACGCCGAA GCCGGCAGAA TCCAGCAGGA AATTCAGCGA ATCCCGCATC GCCGCGTCGT CGTCGATGAC GTAGATATGT CCCTTGGTCG TCATGCTAGA CCTCCTATCA TCTCGTCGGC TGCCGGGAGG GTGAAGCGGA AGGTCGCCCC GCCCGATGCG TTGCTCTCGG CCCACATGCG CCCGCCGTGA GCTTCGATGA TCGAGCGGCT GATGGACAGT CCCACGCCCA TGCCGGTGTC CTTGGTGGTG AAGAAAGTCT GAAACAGGTT CGGAATGACG TCGTCCTGGA AACCGCTGCC GGTGTCGGAC ACTTCGACCT CGATCATGTC GTCGGCGGCG GGGGTGTTGG TGACGACGAG CTCGCGTCGC TGCGACTGAG CCATCGCTTC CAGCGCGTTG CGGAACAGGT TGACCAGGAC CTGCTGGATC TGCACCCGGT CGGCGAGAAC GAGATCGGCG CCCGGATCGA GACTGAAGCG GAGCTGCACG TTCTGCTCGC GCGCGCCGGC AAGCCCGAGC GCGCCGGCCT CCTCGATCAG CTTGGAGAGA CTCTCGACCC GCTTCTCCGA TTCGCCGCGG GCAACGAAGT CGCGCAGGCG CCGGATGATC TGGCCGGCGC GCAGCGCCTG CTCGGCGGCG CGGTCCAGGG CGCTTTCGAC CTTCGGTGTG TTCGGATCAC TGCTGCCGGC AAGCAGCCGC CGCGAGCCCT TCATGTAGTT GCTGATCGCC GCCAGCGGCT GGTTGAGCTC GTGCGCGAGC GCGGACGCCA TTTCGCCCAT GGCGCTCAGC CTGGAGACGT GGACGAGCTC GGATTGCAGT TCCTGGAGGC GCGCCTGGGT CTGCTGGTGC TCGGTGATAT CATTCTGAAT ACCGACAAAA TACGTTTTAT CCTCTATTTC CATTGGATCA ATATTTAATT CATTCCAGAA CATCGTTCCG TCTTTTTTGT AGTTTTGGAT CTGAACGGTG ACCGGTTCTT TATTTTGTAA AGCGGTTCTG ATGTTGTCCA CTTCTGCAGG ATCTGTGTGT TTCCCCTGTA AGAAGCGACA GTTCTTTCCT AAAATTTCCT CGGTCTCGTA GCCGGTCATT TGAACAAAGC CTTGATTTAC GTAGACAATA GGATTATCTT CAAGTGCGGG ATCTGTAATT ACCACACCGA CTCGCACGTG ATCAAGTGCT TTTTTGATGA CTTCCAGCTG TCCTGGTATC CCAAATGATT GAAAACTAGC CACATTCACC ACCCTCAATT GACTCTCTTC CGGGCGCTAT CATGCCATAC CGCGAAAGGT TTTGCACCAT TCGATGGTGT CCGGGATCTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTACGCC CGTGATCCTG ATCACCGGCT ATCCGGACGA AAACATCTCG ACCCGGGCCG CCGAGGCCGG CGTAAAAGAC GTGGTTTTGA AGCCGCTTCT CGACGAAAAC CTGCTCAAGC GTATCCGCCG CGCCATCCAG GACCGGCCTC GGGCATGACC TACGGGGTTC TACGTAAGGC ACCCCCCTTA AGATATCGCT CGAAATTTTC GAACCTCCCG ATACCGCGTA CCAATGCGTC ATCACAACGG AGTCTAGAAA AGAGGAGAAA TACTAGATGA GCACAAAAAA GAAACCATTA ACACAAGAGC AGCTTGAGGA CGCACGTCGC CTTAAAGCAA TTTATGAAAA AAAGAAAAAT GAACTTGGCT TATCCCAGGA ATCTGTCGCA GACAAGATGG GGATGGGGCA GTCAGGCGTT GGTGCTTTAT TTAATGGCAT CAATGCATTA AATGCTTATA ACGCCGCATT GCTTGCAAAA ATTCTCAAAG TTAGCGTTGA AGAATTTAGC CCTTCAATCG CCAGAGAAAT CTACGAGATG TATGAAGCGG TTAGTATGCA GCCGTCACTT AGAAGTGAGT ATGAGTACCC TGTTTTTTCT CATGTTCAGG CAGGGATGTT CTCACCTGAG CTTAGAACCT TTACCAAAGG TGATGCGGAG AGATGGGTAA GCACAACCAA AAAAGCCAGT GATTCTGCAT TCTGGCTTGA GGTTGAAGGT AATTCCATGA CCGCACCAAC AGGCTCCAAG CCGAGCTTTC CTGACGGAAT GTTAATTCTC GTTGACCCTG AGCAGGCTGT TGAGCCAGGT GATTTCTGCA TAGCCAGACT TGGGGGTGAT GAGTTTACCT TCAAGAAACT GATCAGGGAT AGCGGTCAGG TGTTTTTACA ACCACTAAAC CCACAGTACC CAATGATCCC ATGCAATGAG AGTTGTTCCG TTGTGGGGAA AGTTATCGCT AGTCAGTGGC CTGAAGAGAC GTTTGGCGCT GCAAACGACG AAAACTACGC TTTAGTAGCT TAATAACGCT GATAGTGCTA GTGTAGATCG CTACTAGAGC CAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT TTATCTGTTG TTTGTCGGTG AACGCTCTCT ACTAGAGTCA CACTGGCTCA CCTTCGGGTG GGCCTTTCTG CGTTTATATA CTAGAGTAAC ACCGTGCGTG TTGACTATTT TACCTCTGGC GGTGATAATG GTTGCTCTAG AAATAATTTT GTTTAACTTT AAGAAGGAGA TATACCATGG GCAGCAGCCA TCATCATCAT CATCACAGCA GCGGCCTGGT GCCGCGCGGC AGCCATATGG CTAGCATGAC TGGTGGACAG CAAATGGGTC GGATCCATAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGTGAA AGCTTGCGGC CGCACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGAT
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