pEF-hSEMA4C-Fc
$113.00
In stock
SKU
PL81829
SEMAF;SEMAI;SEMACL1;M-SEMA-F
100μg liquid plasmid(Instantly):$134
| name | pEF-hSEMA4C-Fc | another name | apEF-hSEMA4C-Fc |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTT AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTTGCAA AGATGGATAA AGTTTTAAAC AGAGAGGAAT CTTTGCAGCT AATGGACCTT CTAGGTCTTG AAAGGAGTGC CTCGTGAGGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACCGG TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG TTTGCCGCCA GAACACAGGT AAGTGCCGTG TGTGGTTCCC GCGGGCCTGG CCTCTTTACG GGTTATGGCC CTTGCGTGCC TTGAATTACT TCCACCTGGC TGCAGTACGT GATTCTTGAT CCCGAGCTTC GGGTTGGAAG TGGGTGGGAG AGTTCGAGGC CTTGCGCTTA AGGAGCCCCT TCGCCTCGTG CTTGAGTTGA GGCCTGGCCT GGGCGCTGGG GCCGCCGCGT GCGAATCTGG TGGCACCTTC GCGCCTGTCT CGCTGCTTTC GATAAGTCTC TAGCCATTTA AAATTTTTGA TGACCTGCTG CGACGCTTTT TTTCTGGCAA GATAGTCTTG TAAATGCGGG CCAAGATCTG CACACTGGTA TTTCGGTTTT TGGGGCCGCG GGCGGCGACG GGGCCCGTGC GTCCCAGCGC ACATGTTCGG CGAGGCGGGG CCTGCGAGCG CGGCCACCGA GAATCGGACG GGGGTAGTCT CAAGCTGGCC GGCCTGCTCT GGTGCCTGGC CTCGCGCCGC CGTGTATCGC CCCGCCCTGG GCGGCAAGGC TGGCCCGGTC GGCACCAGTT GCGTGAGCGG AAAGATGGCC GCTTCCCGGC CCTGCTGCAG GGAGCTCAAA ATGGAGGACG CGGCGCTCGG GAGAGCGGGC GGGTGAGTCA CCCACACAAA GGAAAAGGGC CTTTCCGTCC TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGAA TTCTCTAGAG ATCCCTCGAC CTCGAGATCC ATCGGGCCCC CCCTCGAGGT CGACTCGCCT CTACCTGATG GCCCCACACT GGGCTGTCTG GCTGCTGGCA GCAAGGCTGT GGGGCCTGGG CATTGGGGCT GAGGTGTGGT GGAACCTTGT GCCGCGTAAG ACAGTGTCTT CTGGGGAGCT GGCCACGGTA GTACGGCGGT TCTCCCAGAC CGGCATCCAG GACTTCCTGA CACTGACGCT GACGGAGCCC ACTGGGCTTC TGTACGTGGG CGCCCGAGAG GCCCTGTTTG CCTTCAGCAT GGAGGCCCTG GAGCTGCAAG GAGCGATCTC CTGGGAGGCC CCCGTGGAGA AGAAGACTGA GTGTATCCAG AAAGGGAAGA ACAACCAGAC CGAGTGCTTC AACTTCATCC GCTTCCTGCA GCCCTACAAT GCCTCCCACC TGTACGTCTG TGGCACCTAC GCCTTCCAGC CCAAGTGCAC CTACGTCAAC ATGCTCACCT TCACTTTGGA GCATGGAGAG TTTGAAGATG GGAAGGGCAA GTGTCCCTAT GACCCAGCTA AGGGCCATGC TGGCCTTCTT GTGGATGGTG AGCTGTACTC GGCCACACTC AACAACTTCC TGGGCACGGA ACCCATTATC CTGCGTAACA TGGGGCCCCA CCACTCCATG AAGACAGAGT ACCTGGCCTT TTGGCTCAAC GAACCTCACT TTGTAGGCTC TGCCTATGTA CCTGAGAGTG TGGGCAGCTT CACGGGGGAC GACGACAAGG TCTACTTCTT CTTCAGGGAG CGGGCAGTGG AGTCCGACTG CTATGCCGAG CAGGTGGTGG CTCGTGTGGC CCGTGTCTGC AAGGGCGATA TGGGGGGCGC ACGGACCCTG CAGAGGAAGT GGACCACGTT CCTGAAGGCG CGGCTGGCAT GCTCTGCCCC GAACTGGCAG CTCTACTTCA ACCAGCTGCA GGCGATGCAC ACCCTGCAGG ACACCTCCTG GCACAACACC ACCTTCTTTG GGGTTTTTCA AGCACAGTGG GGTGACATGT ACCTGTCGGC CATCTGTGAG TACCAGTTGG AAGAGATCCA GCGGGTGTTT GAGGGCCCCT ATAAGGAGTA CCATGAGGAA GCCCAGAAGT GGGACCGCTA CACTGACCCT GTACCCAGCC CTCGGCCTGG CTCGTGCATT AACAACTGGC ATCGGCGCCA CGGCTACACC AGCTCCCTGG AGCTACCCGA CAACATCCTC AACTTCGTCA AGAAGCACCC GCTGATGGAG GAGCAGGTGG GGCCTCGGTG GAGCCGCCCC CTGCTCGTGA AGAAGGGCAC CAACTTCACC CACCTGGTGG CCGACCGGGT TACAGGACTT GATGGAGCCA CCTATACAGT GCTGTTCATT GGCACAGGAG ACGGCTGGCT GCTCAAGGCT GTGAGCCTGG GGCCCTGGGT TCACCTGATT GAGGAGCTGC AGCTGTTTGA CCAGGAGCCC ATGAGAAGCC TGGTGCTATC TCAGAGCAAG AAGCTGCTCT TTGCCGGCTC CCGCTCTCAG CTGGTGCAGC TGCCCGTGGC CGACTGCATG AAGTATCGCT CCTGTGCAGA CTGTGTCCTC GCCCGGGACC CCTATTGCGC CTGGAGCGTC AACACCAGCC GCTGTGTGGC CGTGGGTGGC CACTCTGGAT CTCTACTGAT CCAGCATGTG ATGACCTCGG ACACTTCAGG CATCTGCAAC CTCCGTGGCA GTAAGAAAGT CAGGCCCACT CCCAAAAACA TCACGGTGGT GGCGGGCACA GACCTGGTGC TGCCCTGCCA CCTCTCCTCC AACTTGGCCC ATGCCCGCTG GACCTTTGGG GGCCGGGACC TGCCTGCGGA ACAGCCCGGG TCCTTCCTCT ACGATGCCCG GCTCCAGGCC CTGGTTGTGA TGGCTGCCCA GCCCCGCCAT GCCGGGGCCT ACCACTGCTT TTCAGAGGAG CAGGGGGCGC GGCTGGCTGC TGAAGGCTAC CTTGTGGCTG TCGTGGCAGG CCCGTCGGTG ACCTTGGAGG CCCGGGCCCC CCTGGAAAAC CAAAGTAAGT AGGATCCACT AGTTCTATCC TGCCTGGACG CATCCCGGCT ATGCAGTCCC AGTCCAGGGC AGCAAGGCAG GCCCCGTCTG CCTCTTCACC CGGAGGCCTC TGCCCGCCCC ACTCATGCTC AGGGAGAGGG TCTTCTGGCT TTTTCCCCAG GCTCTGGGCA GGCACAGGCT AGGTGCCCCT AACCCAGGCC CTGCACACAA AGGGGCAGGT GCTGGGCTCA GACCTGCCAA GAGCCATATC CGGGAGGACC CTGCCCCTGA CCTAAGCCCA CCCCAAAGGC CAAACTCTCC ACTCCCTCAG CTCGGACACC TTCTCTCCTC CCAGATTCCA GTAACTCCCA ATCTTCTCTC TGCAGAGCCC AAATCTTGTG ACAAAACTCA CACATGCCCA CCGTGCCCAG GTAAGCCAGC CCAGGCCTCG CCCTCCAGCT CAAGGCGGGA CAGGTGCCCT AGAGTAGCCT GCATCCAGGG ACAGGCCCCA GCCGGGTGCT GACACGTCCA CCTCCATCTC TTCCTCAGCA CCTGAACTCC TGGGGGGACC GTCAGTCTTC CTCTTCCCCC CAAAACCCAA GGACACCCTC ATGATCTCCC GGACCCCTGA GGTCACATGC GTGGTGGTGG ACGTGAGCCA CGAAGACCCT GAGGTCAAGT TCAACTGGTA CGTGGACGGC GTGGAGGTGC ATAATGCCAA GACAAAGCCG CGGGAGGAGC AGTACAACAG CACGTACCGT GTGGTCAGCG TCCTCACCGT CCTGCACCAG GACTGGCTGA ATGGCAAGGA GTACAAGTGC AAGGTCTCCA ACAAAGCCCT CCCAGCCCCC ATCGAGAAAA CCATCTCCAA AGCCAAAGGT GGGACCCGTG GGGTGCGAGG GCCACATGGA CAGAGGCCGG CTCGGCCCAC CCTCTGCCCT GAGAGTGACC GCTGTACCAA CCTCTGTCCC TACAGGGCAG CCCCGAGAAC CACAGGTGTA CACCCTGCCC CCATCCCGGG AGGAGATGAC CAAGAACCAG GTCAGCCTGA CCTGCCTGGT CAAAGGCTTC TATCCCAGCG ACATCGCCGT GGAGTGGGAG AGCAATGGGC AGCCGGAGAA CAACTACAAG ACCACGCCTC CCGTGCTGGA CTCCGACGGC TCCTTCTTCC TCTATAGCAA GCTCACCGTG GACAAGAGCA GGTGGCAGCA GGGGAACGTC TTCTCATGCT CCGTGATGCA TGAGGCTCTG CACAACCACT ACACGCAGAA GAGCCTCTCC CTGTCCCCGG GTAAATGAGT GCGACGGCCG GCAAGCCCCC GCTCCCCGGG CTCTCGCGGT CGCACGAGGA TGCTTGGCAC GTACCCCGTC TACATACTTC CCAGGCACCC AGCATGGAAA TAAAGCACCC ACCACTGCCC TAGAGCGGCC GCGACTCTAG AGTGAGGGTC CCCACCTGGG ACCCTTGAGA GTATCAGGTC TCCCACGTGG GAGACAAGAA ATCCCTGTTT AATATTTAAA CAGCAGTGTT CCCCATCTGG GTCCTTGCAC CCCTCACTCT GGCCTCAGCC GACTGCACAG CGGCCCCTGC ATCCCCTTGG CTGTGAGGCC CCTGGACAAG CAGAGGTGGC CAGAGCTGGG AGGCATGGCC CTGGGGTCCC ACGAATTTGC TGGGGAATCT CGTTTTTCTT CTTAAGACTT TTGGGACATG GTTTGACTCC CGAACATCAC CGACGCGTCT CCTGTTTTTC TGGGTGGCCT CGGGACACCT GCCCTGCCCC CACGAGGGTC AGGACTGTGA CTCTTTTTAG GGCCAGGCAG GTGCCTGGAC ATTTGCCTTG CTGGACGGGG ACTGGGGATG TGGGAGGGAG CAGACAGGAG GAATCATGTC AGGCCTGTGT GTGAAAGGAA GCTCCACTGT CACCCTCCAC CTCTTCACCC CCCACTCACC AGTGTCCCCT CCACTGTCAC ATTGTAACTG AACTTCAGGA TAATAAAGTG CTTGCCTCCA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAG AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCAAAACGT TAATATTTTG TTAAAATTCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG ATGGTGGTTC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGCCC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCAAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT ACTATGGTTG CTTTGACGAG CATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGA
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