GeneArt CRISPR Nuclease Vector-OFP-gRNA
$113.00
In stock
SKU
PL81824
100μg liquid plasmid(Instantly):$134
| name | GeneArt CRISPR Nuclease Vector-OFP-gRNA | another name | aGeneArt CRISPR Nuclease Vector-OFP-gRNA |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG AGCGGCCGCC ACCATGGGCA AGCCCATCCC TAACCCCCTG TTGGGGCTGG ACAGCACCGC TCCCAAAAAG AAAAGGAAGG TGGGCATTCA CGGCGTGCCT GCGGCCGACA AAAAGTACAG CATCGGCCTT GATATCGGCA CCAATAGCGT GGGCTGGGCC GTTATCACAG ACGAATACAA GGTACCCAGC AAGAAGTTCA AGGTGCTGGG GAATACAGAC AGGCACTCTA TCAAGAAAAA CCTTATCGGG GCTCTGCTGT TTGACTCAGG CGAGACCGCC GAGGCCACCA GGTTGAAGAG GACCGCAAGG CGAAGGTACA CCCGGAGGAA GAACAGGATC TGCTATCTGC AGGAGATCTT CAGCAACGAG ATGGCCAAGG TGGACGACAG CTTCTTCCAC AGGCTGGAGG AGAGCTTCCT TGTCGAGGAG GATAAGAAGC ACGAACGACA CCCCATCTTC GGCAACATAG TCGACGAGGT CGCTTATCAC GAGAAGTACC CCACCATCTA CCACCTGCGA AAGAAATTGG TGGATAGCAC CGATAAAGCC GACTTGCGAC TTATCTACTT GGCTCTGGCG CACATGATTA AGTTCAGGGG CCACTTCCTG ATCGAGGGCG ACCTTAACCC CGACAACAGT GACGTAGACA AATTGTTCAT CCAGCTTGTA CAGACCTATA ACCAGCTGTT CGAGGAAAAC CCTATTAACG CCAGCGGGGT GGATGCGAAG GCCATACTTA GCGCCAGGCT GAGCAAAAGC AGGCGCTTGG AGAACCTGAT AGCCCAGCTG CCCGGTGAAA AGAAGAACGG CCTCTTCGGT AATCTGATTG CCCTGAGCCT GGGCCTGACC CCCAACTTCA AGAGCAACTT CGACCTGGCA GAAGATGCCA AGCTGCAGTT GAGTAAGGAC ACCTATGACG ACGACTTGGA CAATCTGCTC GCCCAAATCG GCGACCAGTA CGCTGACCTG TTCCTCGCCG CCAAGAACCT TTCTGACGCA ATCCTGCTTA GCGATATCCT TAGGGTGAAC ACAGAGATCA CCAAGGCCCC CCTGAGCGCC AGCATGATCA AGAGGTACGA CGAGCACCAT CAGGACCTGA CCCTTCTGAA GGCCCTGGTG AGGCAGCAAC TGCCCGAGAA GTACAAGGAG ATCTTTTTCG ACCAGAGCAA GAACGGCTAC GCCGGCTACA TCGACGGCGG AGCCAGCCAA GAGGAGTTCT ACAAGTTCAT CAAGCCCATC CTGGAGAAGA TGGATGGCAC CGAGGAGCTG CTGGTGAAGC TGAACAGGGA AGATTTGCTC CGGAAGCAGA GGACCTTTGA CAACGGTAGC ATCCCCCACC AGATCCACCT GGGCGAGCTG CACGCAATAC TGAGGCGACA GGAGGATTTC TACCCCTTCC TCAAGGACAA TAGGGAGAAA ATCGAAAAGA TTCTGACCTT CAGGATCCCC TACTACGTGG GCCCTCTTGC CAGGGGCAAC AGCCGATTCG CTTGGATGAC AAGAAAGAGC GAGGAGACCA TCACCCCCTG GAACTTCGAG GAAGTGGTGG ACAAAGGAGC AAGCGCGCAG TCTTTCATCG AACGGATGAC CAATTTCGAC AAAAACCTGC CTAACGAGAA GGTGCTGCCC AAGCACAGCC TGCTTTACGA GTACTTCACC GTGTACAACG AGCTCACCAA GGTGAAATAT GTGACCGAGG GCATGCGAAA ACCCGCTTTC CTGAGCGGCG AGCAGAAGAA GGCCATCGTG GACCTGCTGT TCAAGACCAA CAGGAAGGTG ACCGTGAAGC AGCTGAAGGA GGACTACTTC AAGAAGATCG AGTGCTTTGA TAGCGTGGAA ATAAGCGGCG TGGAGGACAG GTTCAACGCC AGCCTGGGCA CCTACCACGA CTTGTTGAAG ATAATCAAAG ACAAGGATTT CCTGGATAAT GAGGAGAACG AGGATATACT CGAGGACATC GTGCTGACTT TGACCCTGTT TGAGGACCGA GAGATGATTG AAGAAAGGCT CAAAACCTAC GCCCACCTGT TCGACGACAA AGTGATGAAA CAACTGAAGA GACGAAGATA CACCGGCTGG GGCAGACTGT CCAGGAAGCT CATCAACGGC ATTAGGGACA AGCAGAGCGG CAAGACCATC CTGGATTTCC TGAAGTCCGA CGGCTTCGCC AACCGAAACT TCATGCAGCT GATTCACGAT GACAGCTTGA CCTTCAAGGA GGACATCCAG AAGGCCCAGG TTAGCGGCCA GGGCGACTCC CTGCACGAAC ATATTGCAAA CCTGGCAGGC TCCCCTGCGA TCAAGAAGGG CATACTGCAG ACCGTTAAGG TTGTGGACGA ATTGGTCAAG GTCATGGGCA GGCACAAGCC CGAAAACATA GTTATAGAGA TGGCCAGAGA GAACCAGACC ACCCAAAAGG GCCAGAAGAA CAGCCGGGAG CGCATGAAAA GGATCGAGGA GGGTATCAAG GAACTCGGAA GCCAGATCCT CAAAGAGCAC CCCGTGGAGA ATACCCAGCT CCAGAACGAG AAGCTGTACC TGTACTACCT GCAGAACGGC AGGGACATGT ACGTTGACCA GGAGTTGGAC ATCAACAGGC TTTCAGACTA TGACGTGGAT CACATAGTGC CCCAGAGCTT TCTTAAAGAC GATAGCATCG ACAACAAGGT CCTGACCCGC TCCGACAAAA ACAGGGGCAA AAGCGACAAC GTGCCAAGCG AAGAGGTGGT TAAAAAGATG AAGAACTACT GGAGGCAACT GCTCAACGCG AAATTGATCA CCCAGAGAAA GTTCGATAAC CTGACCAAGG CCGAGAGGGG CGGACTCTCC GAACTTGACA AAGCGGGCTT CATAAAGAGG CAGCTGGTCG AGACCCGACA GATCACGAAG CACGTGGCCC AAATCCTCGA CAGCAGAATG AATACCAAGT ACGATGAGAA TGACAAACTC ATCAGGGAAG TGAAAGTGAT TACCCTGAAG AGCAAGTTGG TGTCCGACTT TCGCAAAGAT TTCCAGTTCT ACAAGGTGAG GGAGATCAAC AACTACCACC ATGCCCACGA CGCATACCTG AACGCCGTGG TCGGCACCGC CCTGATTAAG AAGTATCCAA AGCTGGAGTC CGAATTTGTC TACGGCGACT ACAAAGTTTA CGATGTGAGG AAGATGATCG CTAAGAGCGA ACAGGAGATC GGCAAGGCCA CCGCTAAGTA TTTCTTCTAC AGCAACATCA TGAACTTTTT CAAGACCGAG ATCACACTTG CCAACGGCGA AATCAGGAAG AGGCCGCTTA TCGAGACCAA CGGTGAGACC GGCGAGATCG TGTGGGACAA GGGCAGGGAC TTCGCCACCG TGAGGAAAGT CCTGAGCATG CCCCAGGTGA ATATTGTGAA AAAAACTGAG GTGCAGACAG GCGGCTTTAG CAAGGAATCC ATCCTGCCCA AGAGGAACAG CGACAAGCTG ATCGCCCGGA AGAAGGACTG GGACCCTAAG AAGTATGGAG GCTTCGACAG CCCCACCGTA GCCTACAGCG TGCTGGTGGT CGCGAAGGTA GAGAAGGGGA AGAGCAAGAA ACTGAAGAGC GTGAAGGAGC TGCTCGGCAT AACCATCATG GAGAGGTCCA GCTTTGAGAA GAACCCCATT GACTTTTTGG AAGCCAAGGG CTACAAAGAG GTCAAAAAGG ACCTGATCAT CAAACTCCCC AAGTACTCCC TGTTTGAATT GGAGAACGGC AGAAAGAGGA TGCTGGCGAG CGCTGGGGAA CTGCAAAAGG GCAACGAACT GGCGCTGCCC AGCAAGTACG TGAATTTTCT GTACCTGGCG TCCCACTACG AAAAGCTGAA AGGCAGCCCC GAGGACAACG AGCAGAAGCA GCTGTTCGTG GAGCAGCACA AGCATTACCT GGACGAGATA ATCGAGCAAA TCAGCGAGTT CAGCAAGAGG GTGATTCTGG CCGACGCGAA CCTGGATAAG GTCCTCAGCG CCTACAACAA GCACCGAGAC AAACCCATCA GGGAGCAGGC CGAGAATATC ATACACCTGT TCACCCTGAC AAATCTGGGC GCACCTGCGG CATTCAAATA CTTCGATACC ACCATCGACA GGAAAAGGTA CACTAGCACT AAGGAGGTGC TGGATGCCAC CTTGATCCAC CAGTCCATTA CCGGCCTGTA TGAGACCAGG ATCGACCTGA GCCAGCTTGG AGGCGACTCT AGGGCGGACC CAAAAAAGAA AAGGAAGGTG GAATTCTCTA GAGGCAGTGG AGAGGGCAGA GGAAGTCTGC TAACATGCGG TGACGTCGAG GAGAATCCTG GCCCAATGAA CCTGAGCAAA AACGTGAGCG TGAGCGTGTA TATGAAGGGG AACGTCAACA ATCATGAGTT TGAGTACGAC GGGGAAGGTG GTGGTGATCC TTATACAGGT AAATATTCCA TGAAGATGAC GCTACGTGGT CAAAATTCCC TACCCTTTTC CTATGATATC ATTACCACGG CATTTCAGTA TGGTTTCCGC GTATTTACAA AATACCCTGA GGGAATTGTT GACTATTTTA AGGACTCGCT TCCCGACGCA TTCCAGTGGA ACAGACGAAT TGTGTTTGAA GATGGTGGAG TACTAAACAT GAGCAGTGAT ATCACATATA AAGATAATGT TCTGCATGGT GACGTCAAGG CTGAGGGAGT GAACTTCCCG CCGAATGGGC CAGTGATGAA GAATGAAATT GTGATGGAGG AACCGACTGA AGAAACATTT ACTCCAAAAA ACGGGGTTCT TGTTGGCTTT TGTCCCAAAG CGTACTTACT TAAAGACGGT TCCTATTACT ATGGAAATAT GACAACATTT TACAGATCCA AGAAATCTGG CCAGGCACCT CCTGGGTATC ACTTTGTTAA GCATCGTCTC GTCAAGACCA ATGTGGGACA TGGATTTAAG ACGGTTGAGC AGACTGAATA TGCCACTGCT CATGTCAGTG ATCTTCCCAA GTTCGAAGCT TGATAATGAG TTTAAACGGG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG AACCCGCGCT ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCA GATCTGCGCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTAAG GTCGGGCAGG AAGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACAC CGTCAAGAGG AACAAGCTTT ACTCAAGAGG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TCTAGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT GCTTAGG
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