pAC5.1b-EGFP-V5-6×His
$128.00
In stock
SKU
PL81813
100μg liquid plasmid(Instantly):$149
| name | pAC5.1b-EGFP-V5-6×His | another name | apAC5.1b-EGFP-V5-6×His |
| Insert Size (bp) | Plasmid host | Insect cells | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | Ac5 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTTCAAGGAA TTAATTCTAT ATTCTAAAAA CACAAATGAT ACTTCTAAAA AAAATCATGA ATGGCATCAA CTCTGAATCA AATCTTTGCA GATGCACCTA CTTCTCATTT CCACTGTCAC ATCATTTTTC CAGATCTCGC TGCCTGTTAT GTGGCCCACA AACCAAGACA CGTTTTATGG CCATTAAAGC TGGCTGATCG TCGCCAAACA CCAAATACAT ATCAATATGT ACATTCGAGA AAGAAGCGAT CAAAGAAGCG TCTTCGGGCG AGTAGGAGAA TGCGGAGGAG AAGGAGAACG AGCTGATCTA GTATCTCTCC ACAATCCAAT GCCAACTGAC CAACTGGCCA TATTCGGAGC AATTTGAAGC CAATTTCCAT CGCCTGGCGA TCGCTCCATT CTTGGCTATA TGTTTTTCAC CGTTCCCGGG GCCATTTTCA AAGACTCGTC GGTAAGATAA GATTGTGTCA CTCGCTGTCT CTCTTCATTT GTCGAAGAAT GCTGAGGAAT TTCGCGATGA CGTCGGCGAG TATTTTGAAG AATGAGAATA ATTTGTATTT ATACGAAAAT CAGTTAGTGG AATTTTCTAC AAAAACATGT TATCTATAGA TAATTTTGTT GCAAAATATG TTGACTATGA CAAAGATTGT ATGTATATAC CTTTAATGTA TTCTCATTTT CTTATGTATT TATAATGGCA ATGATGATAC TGATGATATT TTAAGATGAT GCCAGACCAC AGGCTGATTT CTGCGTCTTT TGCCGAACGC AGTGCATGTG CGGTTGTTGT TTTTTGGAAT AGTTTCAATT TTCGGACTGT CCGCTTTGAT TTCAGTTTCT TGGCTTATTC AAAAAGCAAA GTAAAGCCAA AAAAGCGAGA TGGCAATACC AAATGCGGCA AAACGGTAGT GGAAGGAAAG GGGTGCGGGG CAGCGGAAGG AAGGGTGGGG CGGGGCGTGG CGGGGTCTGT GGCTGGGCGC GACGTCACCG ACGTTGGAGC CACTCCTTTG ACCATGTGTG CGTGTGTGTA TTATTCGTGT CTCGCCACTC GCCGGTTGTT TTTTTCTTTT TATCTCGCTC TCTCTAGCGC CATCTCGTAC GCATGCTCAA CGCACCGCAT GTTGCCGTGT CCTTTATGCG TCATTTTGGC TCGAAATAGG CAATTATTTA AACAAAGATT AGTCAACGAA AACGCTAAAA TAAATAAGTC TACAATATGG TTACTTATTG CCATGTGTGT GCAGCCAACG ATAGCAACAA AAGCAACAAC ACAGTGGCTT TCCCTCTTTC ACTTTTTGTT TGCAAGCGCG TGCGAGCAAG ACGGCACGAC CGGCAAACGC AATTACGCTG ACAAAGAGCA GACGAAGTTT TGGCCGAAAA ACATCAAGGC GCCTGATACG AATGCATTTG CAATAACAAT TGCGATATTT AATATTGTTT ATGAAGCTGT TTGACTTCAA AACACACAAA AAAAAAAATA AAACAAATTA TTTGAAAGAG AATTAGGAAT CGGACAGCTT ATCGTTACGG GCTAACAGCA CACCGAGACG AAATAGCTTA CCTGACGTCA CAGCCTCTGG AAGAACTGCC GCCAAGCAGA GAGAGAGAGA AAAAGAGGGA GAGCAGCTTA GACCGCATGT GCTTGTGTGT GAGGCGTCTC TCTCTTCGTC TCCTGTTTGC GCAAACGCAT AGACTGCACT GAGAAAATCG ATTACCTATT TTTTATGAAT GAATATTTGC ACTATTACTA TTCAAAACTA TTAAGATAGC AATCACATTC AATAGCCAAA TACTATACCA CCTGAGCGAT GCAACGAAAT GATCAATTTG AGCAAAAATG CTGCATATTT AGGACGGCAT CATTATAGAA ATGCTTCTTG CTGTGTACTT TTCTCTCGTC TGGCAGCTGT TTCGCCGTTA TTGTTAAAAC CGGCTTAAGT TAGGTGTGTT TTCTACGACT AGTGATGCCC CTACTAGAAG ATGTGTGTTG CACAAATGTC CCTGAATAAC CAATTTGAAG TGCAGATAGC AGTAAACGTA AGCTAATATG AATATTATTT AACTGTAATG TTTTAATATC GCTGGACATT ACTAATAAAC CCACTATAAA CACATGTACA TATGTATGTT TTGGCATACA ATGAGTAGTT GGGGAAAAAA TGTGTAAAAG CACCGTGACC ATCACAGCAT AAAGATAACC AGCTGAAGTA TCGAATATGA GTAACCCCCA AATTGAATCA CATGCCGCAA CTGATAGGAC CCATGGAAGT ACACTCTTCA TGGCGATATA CAAGACACAC ACAAGCACGA ACACCCAGTT GCGGAGGAAA TTCTCCGTAA ATGAAAACCC AATCGGCGAA CAATTCATAC CCATATATGG TAAAAGTTTT GAACGCGACT TGAGAGCGGA GAGCATTGCG GCTGATAAGG TTTTAGCGCT AAGCGGGCTT TATAAAACGG GCTGCGGGAC CAGTTTTCAT ATCACTACCG TTTGAGTTCT TGTGCTGTGT GGATACTCCT CCCGACACAA AGCCGCTCCA TCAGCCAGCA GTCGTCTAAT CCAGAGACCC CGGATCGGGG TACCTACTAG TCCAGTGTGG TGGAATTTCG AGCTTTATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTTTCTAG AGGGCCCGCG GTTCGAAGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGC GTACCGGTCA TCATCACCAT CACCATTGAG TTTAAACCCG CTGATCAGCC TCGACTGTGC CTTCTAAGGC CTGAGCTCGC TGATCAGCCT CGATCGAGGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCA GTCGACCGAT GCCCTTGAGA GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC CGGCAGCGCT CTGGGTCATT TTCGGCGAGG ACCGCTTTCG CTGGAGCGCG ACGATGATCG GCCTGTCGCT TGCGGTATTC GGAATCTTGC ACGCCCTCGC TCAAGCCTTC GTCACTGGTC CCGCCACCAA ACGTTTCGGC GAGAAGCAGG CCATTATCGC CGGCATGGCG GCCGACGCGC TGGGCTACGT CTTGCTGGCG TTCGCGACGC GAGGCTGGAT GGCCTTCCCC ATTATGATTC TTCTCGCTTC CGGCGGCATC GGGATGCCCG CGTTGCAGGC CATGCTGTCC AGGCAGGTAG ATGACGACCA TCAGGGACAG CTTCAAGGAT CGCTCGCGGC TCTTACCAGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTG CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA CACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGT
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