pSH63
$99.00
In stock
SKU
PL81810
100μg liquid plasmid(Instantly):$120
| name | pSH63 | another name | apSH63 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Gene editing | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | TRP1 | Fluorescent labeling | |
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAACG ACATTACTAT ATATATAATA TAGGAAGCAT TTAATAGACA GCATCGTAAT ATATGTGTAC TTTGCAGTTA TGACGCCAGA TGGCAGTAGT GGAAGATATT CTTTATTGAA AAATAGCTTG TCACCTTACG TACAATCTTG ATCCGGAGCT TTTCTTTTTT TGCCGATTAA GAATTAATTC GGTCGAAAAA AGAAAAGGAG AGGGCCAAGA GGGAGGGCAT TGGTGACTAT TGAGCACGTG AGTATACGTG ATTAAGCACA CAAAGGCAGC TTGGAGTATG TCTGTTATTA ATTTCACAGG TAGTTCTGGT CCATTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG CAGAATGTGC TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA AGAGAACAAT TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA ATAGTTCAGG CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG TTTTGGCTCT GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT GGCAAGAATA CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG ACTGCAACAT ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG ATTCAGAAGC AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG GAAGGCAAGA GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA ATGTTGGTGA TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT AGGTTATTAC TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCTA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG AAATTGTAAA CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAG CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT TCAGTATAAT GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA TAAATAACGT TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGACC TAGACTTCAG GTTGTCTAAC TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC GTGACATAAC TAATTACATG ACTCGAGGTC GACAATTCCA ACCTTACCCA AGAGTTCGCC AAACTCAGAC ATCACTTTAN GCAAAACCGC GCCGTGCTTC TTCCTCGGTG GCATTCATCA CGAAATGTTC AGCACTACGC ATACTTTTGA CAGGAAACGC AACGGATATT GAGTCAATAT CAGGCATTCT ATCGCTCAGC TTTACAGTGA CAATGACGGC TGGCGACTGA ATATTAGTGC TTACAGACAG CACTACATAT TTTCCGTCGA TGTTGAAATC CTTTCTCATA TGTCACCATA AATATCAAAT AATTATAGCA ATCATTTACG CGTTAATGGC TAATCGCCAT CTTCCAGCAG GCGCACCATT GCCCCTGTTT CACTATCCAG GGTACGGATA TAGTTCATGA CAATATTTAC ATTGGTCCAG CCACCAGCTT GCATGATCTC CGGTATTGAA ACTCCAGCGC GGGCCATATC TCGCGCGGCT CCGACACGGG CACTGTGTCC AGACCAGGCC AGGTATCTCT GACCAGAGTC ATCCTTAGCG CCGTAAATCA ATCGATGAGT TGCTTCAAAA ATCCCTTCCA GGGCGCGAGT TGATAGCTGG CTGGTGGCAG ATGGCGCGGC AACACCATTT TTTCTGACCC GGCAAAACAG GTAGTTATTC GGATCATCAG CTACACCAGA GACGGAAATC CATCGCTCGA CCAGTTTAGT TACCCCCAGG CTAAGTGCCT TCTCTACACC TGCGGTGCTA ACCAGCGTTT TCGTTCTGCC AATATGGATT AACATTCTCC CACCGTCAGT ACGTGAGATA TCTTTAACCC TGATCCTGGC AATTTCGGCT ATACGTAACA GGGTGTTATA AGCAATCCCC AGAAATGCCA GATTACGTAT ATCCTGGCAG CGATCGCTAT TTTCCATGAG TGAACGAACC TGGTCGAAAT CAGTGCGTTC GAACGCTAGA GCCTGTTTTG CACGTTCACC GGCATCAACG TTTTCTTTTC GGATCCGCCG CATAACCAGT GAAACAGCAT TGCTGTCACT TGGTCGTGGC AGCCCGGACC GACGATGAAG CATGTTTAGC TGGCCCAAAT GTTGCTGGAT AGTTTTTACT GCCAGACCGC GCGCCTGAAG ATATAGAAGA TAATCGCGAA CATCTTCAGG TTCTGCGGGA AACCATTTCC GGTTATTCAA CTTGCACCAT GCCGCCCACG ACCGGCAAAC GGACAGAAGC ATTTTCCAGG TATGCTCAGA AAACGCCTGG CGATCCCTGA ACATGTCCAT CAGGTTCTTG CGAACCTCAT CACTCGTTGC ATCGACCGGT AATGCAGGCA AATTTTGGTG TACGGTCAGT AAATTGGACA TTTAACACTC AGATAATGGT TTTAAGTAAA GTGTACAGGA TCGGCTCTGC CCCTCGACGG TATCGATAAG CTTGATATCG AATTCCTGCA GCCCGGGGGA TCCACTAGTT CTAGAATCCG GGGTTTTTTC TCCTTGACGT TAAAGTATAG AGGTATATTA ACAATTTTTT GTTGATACTT TTATTACATT TGAATAAGAA GTAATACAAA CCGAAAATGT TGAAAGTATT AGTTAAAGTG GTTATGCAGT TTTTGCATTT ATATATCTGT TAATAGATCA AAAATCATCG CTTCGCTGAT TAATTACCCC AGAAATAAGG CTAAAAAACT AATCGCATTA TCATCCTATG GTTGTTAATT TGATTCGTTC ATTTGAAGGT TTGTGGGGCC AGGTTACTGC CAATTTTTCC TCTTCATAAC CATAAAAGCT AGTATTGTAG AATCTTTATT GTTCGGAGCA GTGCGGCGCG AGGCACATCT GCGTTTCAGG AACGCGACCG GTGAAGACGA GGACGCACGG AGGAGAGTCT TCCTTCGGAG GGCTGTCACC CGCTCGGCGG CTTCTAATCC GTACTAGAGC TCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATAGG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGGTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGGGTC CTTTTCATCA CGTGCTATAA AAATAATTAT AATTTAAATT TTTTAATATA AATATATAAA TTAAAAATAG AAAGTAAAAA AAGAAATTAA AGAAAAAATA GTTTTTGTTT TCCGAAGATG TAAAAGACTC TAGGGGGATC GCCAACAAAT ACTACCTTTT ATCTTGCTCT TCCTGCTCTC AGGTATTAAT GCCGAATTGT TTCATCTTGT CTGTGTAGAA GACCACACAC GAAAATCCTG TGATTTTACA TTTTACTTAT CGTTAATCGA ATGTATATCT ATTTAATCTG CTTTTCTTGT CTAATAAATA TATATGTAAA GTACGCTTTT TGTTGAAATT TTTTAAACCT TTGTTTATTT TTTTTTCTTC ATTCCGTAAC TCTTCTACCT TCTTTATTTA CTTTCTAAAA TCCAAATACA AAACATAAAA ATAAATAAAC ACAGAGTAAA TTCCCAAATT ATTCCATCAT TAAAAGATAC GAGGCGCGTG TAAGTTACAG GCAAGCGATC CGTCCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTC
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