pUAST
$128.00
In stock
SKU
PL81723
100μg liquid plasmid(Instantly):$149
| name | pUAST | another name | apUAST |
| Insert Size (bp) | Plasmid host | Insect cells | |
| Plasmid uses | Gene editing | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AAGCTTGGGC TGCATCTCTC CGGATCCAAG CTTGCATGCC TGCAGGTCGG AGTACTGTCC TCCGAGCGGA GTACTGTCCT CCGAGCGGAG TACTGTCCTC CGAGCGGAGT ACTGTCCTCC GAGCGGAGTA CTGTCCTCCG AGCGGAGACT CTAGCGAGCG CCGGAGTATA AATAGAGGCG CTTCGTCTAC GGAGCGACAA TTCAATTCAA ACAAGCAAAG TGAACACGTC GCTAAGCGAA AGCTAAGCAA ATAAACAAGC GCAGCTGAAC AAGCTAAACA ATCTGCAGTA AAGTGCAAGT TAAAGTGAAT CAATTAAAAG TAACCAGCAA CCAAGTAAAT CAACTGCAAC TACTGAAATC TGCCAAGAAG TAATTATTGA ATACAAGAAG AGAACTCTGA ATAGGGAATT GGGAATTCGT TAACAGATCT TGCGGCCGCG CTCGAGGGTA CCTCTAGAGG ATCTTTGTGA AGGAACCTTA CTTCTGTGGT GTGACATAAT TGGACAAACT ACCTACAGAG ATTTAAAGCT CTAAGGTAAA TATAAAATTT TTAAGTGTAT AATGTGTTAA ACTACTGATT CTAATTGTTT GTGTATTTTA GATTCCAACC TATGGAACTG ATGAATGGGA GCAGTGGTGG AATGCCTTTA ATGAGGAAAA CCTGTTTTGC TCAGAAGAAA TGCCATCTAG TGATGATGAG GCTACTGCTG ACTCTCAACA TTCTACTCCT CCAAAAAAGA AGAGAAAGGT AGAAGACCCC AAGGACTTTC CTTCAGAATT GCTAAGTTTT TTGAGTCATG CTGTGTTTAG TAATAGAACT CTTGCTTGCT TTGCTATTTA CACCACAAAG GAAAAAGCTG CACTGCTATA CAAGAAAATT ATGGAAAAAT ATTTGATGTA TAGTGCCTTG ACTAGAGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGATCCAC TAGAAGGCCT AATTCAATTC TAGTATGTAT GTAAGTTAAT AAAACCCTTT TTTGGAGAAT GTAGATTTAA AAAAACATAT TTTTTTTTTA TTTTTTACTG CACTGGATAT CATTGAACTT ATCTGATCAG TTTTAAATTT ACTTCGATCC AAGGGTATTT GAAGTACCAG GTTCTTTCGA TTACCTCTCA CTCAAAATGA CATTCCACTC AAAGTCAGCG CTGTTTGCCT CCTTCTCTGT CCACAGAAAT ATCGCCGTCT CTTTCGCCGC TGCGTCCGCT ATCTCTTTCG CCACCGTTTG TAGCGTTACC TAGCGTCAAT GTCCGCCTTC AGTTGCACTT TGTCAGCGGT TTCGTGACGA AGCTCCAAGC GGTTTACGCC ATCAATTAAA CACAAAGTGC TGTGCCAAAA CTCCTCTCGC TTCTTATTTT TGTTTGTTTT TTGAGTGATT GGGGTGGTGA TTGGTTTTGG GTGGGTAAGC AGGGGAAAGT GTGAAAAATC CCGGCAATGG GCCAAGAGGA TCAGGAGCTA TTAATTCGCG GAGGCAGCAA ACACCCATCT GCCGAGCATC TGAACAATGT GAGTAGTACA TGTGCATACA TCTTAAGTTC ACTTGATCTA TAGGAACTGC GATTGCAACA TCAAATTGTC TGCGGCGTGA GAACTGCGAC CCACAAAAAT CCCAAACCGC AATCGCACAA ACAAATAGTG ACACGAAACA GATTATTCTG GTAGCTGTGC TCGCTATATA AGACAATTTT TAAGATCATA TCATGATCAA GACATCTAAA GGCATTCATT TTCGACTACA TTCTTTTTTA CAAAAAATAT AACAACCAGA TATTTTAAGC TGATCCTAGA TGCACAAAAA ATAAATAAAA GTATAAACCT ACTTCGTAGG ATACTTCGTT TTGTTCGGGG TTAGATGAGC ATAACGCTTG TAGTTGATAT TTGAGATCCC CTATCATTGC AGGGTGACAG CGGACGCTTC GCAGAGCTGC ATTAACCAGG GCTTCGGGCA GGCCAAAAAC TACGGCACGC TCCTGCCACC CAGTCCGCCG GAGGACTCCG GTTCAGGGAG CGGCCAACTA GCCGAGAACC TCACCTATGC CTGGCACAAT ATGGACATCT TTGGGGCGGT CAATCAGCCG GGCTCCGGAT GGCGGCAGCT GGTCAACCGG ACACGCGGAC TATTCTGCAA CGAGCGACAC ATACCGGCGC CCAGGAAACA TTTGCTCAAG AACGGTGAGT TTCTATTCGC AGTCGGCTGA TCTGTGTGAA ATCTTAATAA AGGGTCCAAT TACCAATTTG AAACTCAGTT TGCGGCGTGG CCTATCCGGG CGAACTTTTG GCCGTGATGG GCAGTTCCGG TGCCGGAAAG ACGACCCTGC TGAATGCCCT TGCCTTTCGA TCGCCGCAGG GCATCCAAGT ATCGCCATCC GGGATGCGAC TGCTCAATGG CCAACCTGTG GACGCCAAGG AGATGCAGGC CAGGTGCGCC TATGTCCAGC AGGATGACCT CTTTATCGGC TCCCTAACGG CCAGGGAACA CCTGATTTTC CAGGCCATGG TGCGGATGCC ACGACATCTG ACCTATCGGC AGCGAGTGGC CCGCGTGGAT CAGGTGATCC AGGAGCTTTC GCTCAGCAAA TGTCAGCACA CGATCATCGG TGTGCCCGGC AGGGTGAAAG GTCTGTCCGG CGGAGAAAGG AAGCGTCTGG CATTCGCCTC CGAGGCACTA ACCGATCCGC CGCTTCTGAT CTGCGATGAG CCCACCTCCG GACTGGACTC ATTTACCGCC CACAGCGTCG TCCAGGTGCT GAAGAAGCTG TCGCAGAAGG GCAAGACCGT CATCCTGACC ATTCATCAGC CGTCTTCCGA GCTGTTTGAG CTCTTTGACA AGATCCTTCT GATGGCCGAG GGCAGGGTAG CTTTCTTGGG CACTCCCAGC GAAGCCGTCG ACTTCTTTTC CTAGTGAGTT CGATGTGTTT ATTAAGGGTA TCTAGCATTA CATTACATCT CAACTCCTAT CCAGCGTGGG TGCCCAGTGT CCTACCAACT ACAATCCGGC GGACTTTTAC GTACAGGTGT TGGCCGTTGT GCCCGGACGG GAGATCGAGT CCCGTGATCG GATCGCCAAG ATATGGCGAC AATTTTGCTA TTAGCAAAGT AGCCCGGGAT ATGGAGCAGT TGTTGGCCAC CAAAAATTTG GAGAAGCCAC TGGAGCAGCC GGAGAATGGG TACACCTACA AGGCCACCTG GTTCATGCAG TTCCGGGCGG TCCTGTGGCG ATCCTGGCTG TCGGTGCTCA AGGAACCACT CCTCGTAAAA GTGCGACTTA TTCAGACAAC GGTGAGTGGT TCCAGTGGAA ACAAATGATA TAACGCTTAC AATTCTTGGA AACAAATTCG CTAGATTTTA GTTAGAATTG CCTGATTCCA CACCCTTCTT AGTTTTTTTC AATGAGATGT ATAGTTTATA GTTTTGCAGA AAATAAATAA ATTTCATTTA ACTCGCGAAC ATGTTGAAGA TATGAATATT AATGAGATGC GAGTAACATT TTAATTTGCA GATGGTTGCC ATCTTGATTG GCCTCATCTT TTTGGGCCAA CAACTCACGC AAGTGGGCGT GATGAATATC AACGGAGCCA TCTTCCTCTT CCTGACCAAC ATGACCTTTC AAAACGTCTT TGCCACGATA AATGTAAGTC TTGTTTAGAA TACATTTGCA TATTAATAAT TTACTAACTT TCTAATGAAT CGATTCGATT TAGGTGTTCA CCTCAGAGCT GCCAGTTTTT ATGAGGGAGG CCCGAAGTCG ACTTTATCGC TGTGACACAT ACTTTCTGGG CAAAACGATT GCCGAATTAC CGCTTTTTCT CACAGTGCCA CTGGTCTTCA CGGCGATTGC CTATCCGATG ATCGGACTGC GGGCCGGAGT GCTGCACTTC TTCAACTGCC TGGCGCTGGT CACTCTGGTG GCCAATGTGT CAACGTCCTT CGGATATCTA ATATCCTGCG CCAGCTCCTC GACCTCGATG GCGCTGTCTG TGGGTCCGCC GGTTATCATA CCATTCCTGC TCTTTGGCGG CTTCTTCTTG AACTCGGGCT CGGTGCCAGT ATACCTCAAA TGGTTGTCGT ACCTCTCATG GTTCCGTTAC GCCAACGAGG GTCTGCTGAT TAACCAATGG GCGGACGTGG AGCCGGGCGA AATTAGCTGC ACATCGTCGA ACACCACGTG CCCCAGTTCG GGCAAGGTCA TCCTGGAGAC GCTTAACTTC TCCGCCGCCG ATCTGCCGCT GGACTACGTG GGTCTGGCCA TTCTCATCGT GAGCTTCCGG GTGCTCGCAT ATCTGGCTCT AAGACTTCGG GCCCGACGCA AGGAGTAGCC GACATATATC CGAAATAACT GCTTGTTTTT TTTTTTACCA TTATTACCAT CGTGTTTACT GTTTATTGCC CCCTCAAAAA GCTAATGTAA TTATATTTGT GCCAATAAAA ACAAGATATG ACCTATAGAA TACAAGTATT TCCCCTTCGA ACATCCCCAC AAGTAGACTT TGGATTTGTC TTCTAACCAA AAGACTTACA CACCTGCATA CCTTACATCA AAAACTCGTT TATCGCTACA TAAAACACCG GGATATATTT TTTATATACA TACTTTTCAA ATCGCGCGCC CTCTTCATAA TTCACCTCCA CCACACCACG TTTCGTAGTT GCTCTTTCGC TGTCTCCCAC CCGCTCTCCG CAACACATTC ACCTTTTGTT CGACGACCTT GGAGCGACTG TCGTTAGTTC CGCGCGATTC GGTTCGCTCA AATGGTTCCG AGTGGTTCAT TTCGTCTCAA TAGAAATTAG TAATAAATAT TTGTATGTAC AATTTATTTG CTCCAATATA TTTGTATATA TTTCCCTCAC AGCTATATTT ATTCTAATTT AATATTATGA CTTTTTAAGG TAATTTTTTG TGACCTGTTC GGAGTGATTA GCGTTACAAT TTGAACTGAA AGTGACATCC AGTGTTTGTT CCTTGTGTAG ATGCATCTCA AAAAAATGGT GGGCATAATA GTGTTGTTTA TATATATCAA AAATAACAAC TATAATAATA AGAATACATT TAATTTAGAA AATGCTTGGA TTTCACTGGA ACTAGAATTA ATTCGGCTGC TGCTCTAAAC GACGCATTTC GTACTCCAAA GTACGAATTT TTTCCCTCAA GCTCTTATTT TCATTAAACA ATGAACAGGA CCTAACGCAC AGTCACGTTA TTGTTTACAT AAATGATTTT TTTTACTATT CAAACTTACT CTGTTTGTGT ACTCCCACTG GTATAGCCTT CTTTTATCTT TTCTGGTTCA GGCTCTATCA CTTTACTAGG TACGGCATCT GCGTTGAGTC GCCTCCTTTT AAATGTCTGA CCTTTTGCAG GTGCAGCCTT CCACTGCGAA TCTTTAAAGT GGGTATCACA AATTTGGGAG TTTTCACCAA GGCTGCACCC AAGGCTCTGC TCCCACAATT TTCTCTTAAT AGCACACTTC GGCACGTGAA TTAATTTTAC TCCAGTCACA GCTTTGCAGC AAAATTTGCA ATATTTCATT TTTTTTTATT CCACGTAAGG GTTAATGTTT TCAAAAAAAA ATTCGTCCGC ACACAACCTT TCCTCTCAAC AAGCAAACGT GCACTGAATT TAAGTGTATA CTTCGGTAAG CTTCGGCTAT CGACGGGACC ACCTTATGTT ATTTCATCAT GGGCCAGACC CACGTAGTCC AGCGGCAGAT CGGCGGCGGA GAAGTTAAGC GTCTCCAGGA TGACCTTGCC CGAACTGGGG CACGTGGTGT TCGACGATGT GCAGCTAATT TCGCCCGGCT CCACGTCCGC CCATTGGTTA ATCAGCAGAC CCTCGTTGGC GTAACGGAAC CATGAGAGGT ACGACAACCA TTTGAGGTAT ACTGGCACCG AGCCCGAGTT CAAGAAGAAG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CCGAATCGCG CGGAACTAAC GACAGTCGCT CCAAGGTCGT CGAACAAAAG GTGAATGTGT TGCGGAGAGC GGGTGGGAGA CAGCGAAAGA GCAACTACGA AACGTGGTGT GGTGGAGGTG AATTATGAAG AGGGCGCGCG ATTTGAAAAG TATGTATATA AAAAATATAT CCCGGTGTTT TATGTAGCGA TAAACGAGTT TTTGATGTAA GGTATGCAGG TGTGTAAGTC TTTTGGTTAG AAGACAAATC CAAAGTCTAC TTGTGGGGAT GTTCGAAGGG GAAATACTTG TATTCTATAG GTCATATCTT GTTTTTATTG GCACAAATAT AATTACATTA GCTTTTTGAG GGGGCAATAA ACAGTAAACA CGATGGTAAT AATGGTAAAA AAAAAAACAA GCAGTTATTT CGGATATATG TCGGCTACTC CTTGCGTCGG GCCCGAAGTC TTAGAGCCAG ATATGCGAGC ACCCGGAAGC TCACGATGAG AATGGCCAGA CCATGATGAA ATAACATAAG GTGGTCCCGT CGGCAAGAGA CATCCACTTA ACGTATGCTT GCAATAAGTG CGAGTGAAAG GAATAGTATT CTGAGTGTCG TATTGAGTCT GAGTGAGACA GCGATATGAT TGTTGATTAA CCCTTAGCAT GTCCGTGGGG TTTGAATTAA CTCATAATAT TAATTAGACG AAATTATTTT TAAAGTTTTA TTTTTAATAA TTTGCGAGTA CGCA
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