Delta 2-3
$128.00
In stock
SKU
PL81720
100μg liquid plasmid(Instantly):$149
| name | Delta 2-3 | another name | aDelta 2-3 |
| Insert Size (bp) | Plasmid host | Insect cells | |
| Plasmid uses | packaging aid | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACATGATTAC GAATTCGAGC TCGGTACCCG GGGATCCGAT CTGAACGGAA TGGCCGTACT GGATGCGTGT GAAAAGTTGA ACAAAAGACT GGCGCCCATC AAGGAGGCAT TGCCTGGAGG CACCTGGAAG GAGTGGATCA ACAAGGCGTA TTTCGATCGG GTCAGCCTCT CGGCCACAGG ATTCTATGCC ATGCCCGGGA TTGGATATCA CCCGGAAACG AATCCCAATG CTCGCACCTA TAGCTACTAC ACGAATGGCG TGGGAGTCAC TGTGGTAGAG ATCGATTGCC TGACTGGCGA CCATCAGGTG CTCAGCACAG ACATCGTGAT GGACATCGGC TCTAGCCTGA ATCCGGCTAT TGACATTGGT CAGATCGAGG GAGCATTCAT GCAGGGCTAT GGACTGTTCA CTTTGGAGGA ACTCATGTAC TCACCACAAG GCATGCTTTA CTCCAGAGGT CCGGGCATGT ACAAGCTGCC AGGATTTGCC GACATTCCCG GGGAGTTCAA TGTCAGCCTA CTGACCGGTG CCCCCAATCC ACGGGCAGTC TACTCTTCCA AGGCAGTGGG TGAACCTCCG CTCTTCATTG GATCATCTGC ATTCTTTGCC ATTAAGGAGG CCATTGCAGC TGCTCGCGAG GATCAGGGCT TGAGTGGTGA CTTCCCACTG GAGGCGCCTT CCACATCGGC ACGCATTCGA ATTGCTTGTC AGGATAAGTT CACGGAACTG GTAAGTTACC CTTGGATTAG TTTAGAACAT TAAACTAACT TTTATTATTA ATTTATTAAT TATAGCTTGA AATACCCGAA CCAGGATCAT TTACGCCATG GAACATTGTG CCTTAAAATT GTTTTTATTG TTTGTACTGC TTAAGCATTT AAAATGACGA TTTTATTTGT TAATTTGTTT ATATATACAT AGTGAATTAA ATGTTTTAAA AATAATTGAG TCGTTTAATG TGTAAACTAA GCTGGAGAGC TGTTTGAACA AATTTATATA CCACTGATTA ATATTAAATA TTCCTTTTAG ATTAAGAAAA ATTCAATGGT TACTTTTTTG TTCTATCTAT TATTCAATAG AATCTCGTGG CATGCAATGC ATTCTGATTT TCAATTGAAA AGTCTTAGTC ACTATTTATT TTGAGTATAT TAATGAAAGT TGAGCAAGTT TTCCGATGAA TTGAAATATG AGTTCCGATT TCGGCGCGAC TGCTACCCGA AAATATAAGC TCAATCAAAA GAAGCTAGCT TGGCTGCAGG TCGATCGACC GTCCCGTCGG CAAGAGACAT CCACTTAACG TATGCTTGCA ATAAGTGCGA GTGAAAGGAA TAGTATTCTG AGTGTCGTAT TGAGTCTGAG TGAGACAGCG ATATGATTGT TGATTAACCC TTAGCATGTC CGTGGGGTTT GAATTAACTC ATAATATTAA TTAGACGAAA TTATTTTTAA AGTTTTATTT TTAATAATTT GGAGTTTCCA ATTAACTTTT GTTTTTGATT TTTAATTTCA ATTTTTTTGT TGAAGTACTT AATTCTAAAA TATATTCTAA TTTTAAAATA AAAAGATTTT ACTTGTTTAT CAACATCGAC GTTTCGCGCT GCTAATATTA ATTTTTCCTT TACATTATTT GTAATTTCAA AATTATTATT ATTTGTATAA TGCAAAAAAA TACATTCTAG CTCTTTTAAC TTCTCTTGAA ATTTTTCGGA CGGCTTAATA AGTCCGCCGT GAGACACCTC GTCGACGTAT GTAAATGTCA AATTTTCTTT TACTTTGTCA CTGATTCTCA ATTTTTTAAT GACATAGCCC GCGATATATT CCATCGCATC CTCCGTCAAC TCATCCATTT CGGTGAAATC TATATTGCTT AACATCTCAT CGACAGGCTC ATCATCGACA GGCTCATCAT CTTTACTTTT ATTTTCGTTT TCTTTAGAAC TGAAATCTAA ATTAAGCCAG CTTTCAGAGT TGTCCTCTTC GATATTTCCC GAATTTCTTA ACATTTCTGT ATTCCTGGCT ATTATATATT TTCTTAACCT ATACTTAAAC TGCAGTGGAG TGGGATGGTC GAATTGTCCA CCTCTCGATC GCATGCTGCC AAAAAAATGC TCCACAATGT CTTGGTTGAG ACGGCTTGTT AATATGTATT GCATACTGAA GTTTTCTTGC AAATATTTAT ACAAGCCATC AAGCGAAGCA TTATTCACAA TGATACCTTT TTGAAATGGG AGCCTTTTGG GTTTATCCAG AATTCCTGTT CGCATAATTT CCGACATTCG ATTCAAAATA TCATTCTGTA TATCCAACTG CTTGCCATAA GGTTGCGAGC ACTCAATGCA ATTGGATGTG GACAATTTAG AATTAAAAAT GTCGAACCAA TCATTCATCA ATTTGAAGAA GTCCGCAGTT TCGGTGGCAT TTTCAATGTC ATACCCCAAT GAATAGCAGC GTCTGATCGA GCTAGCGGTG GTATTCGAAA ACAGCTGGGT AGCCAATTTA ACCTTCTGTT TTGCGAGCGA TCGAACATTA ATGTGATTTT CATTAATTTT AAAGAGGATA GACAGATCGG ACTTGTTGCA AAGATGAAGT GCCTCCTGAA TTGTTTTTTT TGTTAATTTT TTCCCATTTA TTGTTAATCC GGAATCCACA TAGTGGTTAC GGACTAACTT AATTAAATGT GGCGTATCCG AAAAAACGAA AATCTTTAAA TGGTCATCTG CAGGATGGCT AAACCAGGTT TTTGCTAAAA AATGGAATTT CAAGGATTAT CTGATTTTTG TAATATACGA AACTTACATT CTGATATACC GAGCTCTGTC CATAGCTTTT GGTTTCCGGT ACCTAAATCG GATACAATAG CAACTACTAA ATATCCTTTC CTATGCAGTT TCCTTAATAT ATTGTTAAGA GTATCCGGGT CCATTCGGGT ATTAAAATCG AAAAAAACTG GCTGCTTCCA CGATTTTTTT AGACCACGAA CAATAGCCAG TTGGACATAG TCGCTTGGCT CGTAAACAAT ATCAGCAGAG CTGTCATACT CGAAGGCAGC AGCGACCTTC ATCTCGTCGA AAGCGAGTAC GCAAAGCTTG TCGGCGTCAT CAACTCCATC ACTGTCCATT AGGTCTATGA CCACATCCAA ACATCCTCTT TTTATGTCCA CATCTGATAA CCATCTGTAC AAAGTCGTAC GACTGGGCAA AGGAAATCCT TTTTTGTACA GATGGTTATA CGCTCGAGGG CCTGCGGTGT GGAGACAAAT AGCTGTAGAA ATGTCGTCGG AATTGAACGT AGCTCTTTGT CCACCATTCT TCAGTATCCG TATCTGCGTG TCCGTGAAGA TTTTGCGTAG AGACTCCTCC AACTGTTGAG ACTCCCTCAG CTGCTGCTCT AAACGACGCA TTTCGTACTC CAAAGTACGA ATTTTTTCCC TCAAGCTCTT ATTTTCATTA AACAATGAAC AGGACCTAAC GCACAGTCAC GTTATTGTTT ACATAAATGA TTTTTTTTAC TATTCAAACT TACTCTGTTT GTGTACTCCC ACTGGTATAG CCTTCTTTTA TCTTTTCTGG TTCAGGCTCT ATCACTTTAC TAGGTACGGC ATCTGCGTTG AGTCGCCTCC TTTTAAATGT CTGACCTTTT GCAGGTGCAG CCTTCCACTG CGAATCATTA AAGTGGGTAT CACAAATTTG GGAGTTTTCA CCAAGGCTGC ACCCAAGGCT CTGCTCCCAC AATTTTCTCT TAATAGCACA CTTCGGCACG TGAATTAATT TTACTCCAGT CACAGCTTTG CAGCAAAATT TGCAATATTT CATTTTTTTT TATTCCACGT AAGGGTTAAT GTTTTCAAAA AAAAATTCGT CCGCACACAA CCTTTCCTCT CAACAAGCAA ACGTGCACTG AATTTAAGTG TATACTTCGG TAAGCTTATC GAGCTTGGCT GCAGATTGTT TAGCTTGTTC AGCTGCGCTT GTTTATTTGC TTAGCTTTCG CTTAGCGACG TGTTCACTTT GCTTGTTTGA ATTGAATTGT CGCTCCGTAG ACGAAGCGCC TCTATTTATA CTCCGGCGCT CTTTTCGCGA ACATTCGAGG CGCGCTCTCT CGAACCAACG AGAGCAGTAT GCCGTTTACT GTGTGACAGA GTGAGAGAGC ATTAGTGCAG AGAGGGAGAG ACCCAAAAAG AAAAGAGAGA ATAACGAATA ACGGCCAGAG AAATTTCTCG AGTTTTCTTT CTGCCAAACA AATGACCTAC CACAATAACC AGTTTGTTTT GGGATTCTAG AATATTCGCT TTATTTTGGA AATTTCTTTA TAAATACGGC TGCTTAAATA ACTTATGTTA AAGATAATCG AAGCGCTTTG TTATGCGTAT TCCGCCAAAA AAATACTGCG CTTCTAAACC AATAAATGTC TGATCGGCAT ATGTATTAAA GATTATTTTG TACTTTTAGT TAATGAAATC AACTATTAAC GGACTTTCTT CTGTTTTAAT ATACGATAAA ATTAATTTAA GACTCTGAAA AATGTATTAG AATTTAGTTG CGACTTATTT TACATATTTA CATACATATG TACATATGAG GAAAGCCAAG ACTATTACAT ACAGTTTATC AAGTTTACAA ACTGTGAAAT ATGTCGAATT TTTGACTTTA AAGGTAAAAA TTAAGGGTTA TTTGCTTAAT TAAACGGAAT TTTTTTTTTT AATATAATTA AACAATATTT ATTTTACTTA TAAATAAAAA AAAAATTAAA AATATTAGAT ATACAGTTTG TCAAGAAACT GTTTACACAC TGTGAAATAA GTTGAATTTT TGACTTTAAA GCTAAAAATT AAGGGTTTTT TGCTTAATTA AACGCAATTT TTTTATAAAA TATAATTAAA CAATATTTAT TTTACTTATA AATCAAAAAA TAAATTAAAA ATATTAATTA TACAAGAAAA TAAACCACAA ATTCCAAGTT TACACACTTT TGAGACTGTC AAGAAACTCT TTACACAAAA CACTAAATCG AAGTTTAATC CTAAAATTTT AAATTATTTA ATTAAAATTT TAAAAGTTTT AGTACGTAGT ATGTTCCTCA TTGACTTTGT CTACGAGTTG GAGACTGTTT TTATGGAATG GTTACGTTTT TGTAGGTCAT ATTCAAGATC CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGCGCCA CAGGTGCGGT TGCTGGCGCC TATATCGCCG ACATCACCGA TGGGGAAGAT CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCATGCAC CATTCCTTGC GGCGGCGGTG CTCAACGGCC TCAACCTACT ACTGGGCTGC TTCCTAATGC AGGAGTCGCA TAAGGGAGAG CGTCGACGGA TCCTCTAGAG TCGACCTGCA GGCATGCAAG CTTGGCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT T
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