pG-Host9
$128.00
In stock
SKU
PL81718
pGhost9;pG+host9
100μg liquid plasmid(Instantly):$149
| name | pG-Host9 | another name | apG-Host9 |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Ery |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | MC1061 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTAGAGCGGC CGCCACCGCG GTGGGATCCT CTAGAGTCCG CTAGGGACCT CTTTAGCTCC TTGGAAGCTG TCAGTAGTAT ACCTAATAAT TTATCTACAT TCCCTTTAGT AACGTGTAAC TTTCCAAATT TACAAAAGCG ACTCATAGAA TTATTTCCTC CCGTTAAATA ATAGATAACT ATTAAAAATA GACAATACTT GCTCATAAGT AACGGTACTT AAATTGTTTA CTTTGGCGTG TTTCATTGCT TGATGAAACT GATTTTTAGT AAACAGTTGA CGATATTCTC GATTGACCCA TTTTGAAACA AAGTACGTAT ATAGCTTCCA ATATTTATCT GGAACATCTG TGGTATGGCG GGTAAGTTTT ATTAAGACAC TGTTTACTTT TGGTTTAGGA TGAAAGCATT CCGCTGGCAG CTTAAGCAAT TGCTGAATCG AGACTTGAGT GTGCAAGAGC AACCCTAGTG TTCGGTGAAT ATCCAAGGTA CGCTTGTAGA ATCCTTCTTC AACAATCAGA TAGATGTCAG ACGCATGGCT TTCAAAAACC ACTTTTTTAA TAATTTGTGT GCTTAAATGG TAAGGAATAC TCCCAACAAT TTTATACCTC TGTTTGTTAG GGAATTGAAA CTGTAGAATA TCTTGGTGAA TTAAAGTGAC ACGAGTATTC AGTTTTAATT TTTCTGACGA TAAGTTGAAT AGATGACTGT CTAATTCAAT AGACGTTACC TGTTTACTTA TTTTAGCCAG TTTCGTCGTT AAATGCCCTT TACCTGTTCC AATTTCGTAA ACGGTATCGG TTTCTTTTAA ATTCAATTGT TTTATTATTT GGTTGAGTAC TTTTTCACTC GTTAAAAAGT TTTGAGAATA TTTTATATTT TTGTTCATGT AATCACTCCT TCTTAATTAC AAATTTTTAG CATCTAATTT AACTTCAATT CCTATTATAC AAAATTTTAA GATACTGCAC TATCAACACA CTCTTAAGTT TGCTTCTAAG TCTTATTTCC ATAACTTCTT TTACGTTTCC GCCATTCTTT GCTGTTTCGA TTTTTATGAT ATGGTGCAAG TCAGCACGAA CACGAACCGT CTTATCTCCC ATTATATCTT TTTTTGCACT GATTGGTGTA TCATTTCGTT TTTCTTTTGC GCGACTCTAG AGGATCCTGA TAAATATGAA CATGATGAGT GATCGTTAAA TTTATACTGC AATCGGATGC GATTATTGAA TAAAAGATAT GAGAGATTTA TCTAATTTCT TTTTTCTTGT AAAAAAAGAA AGTTCTTAAA GGTTTTATAG TTTTGGTCGT AGAGCACACG GTTTAACGAC TTAATTACGA AGTAAATAAG TCTAGTGTGT TAGACTTTAT GAAATCTATA TACGTTTATA TATATTTATT ATCCGATTTT TTATTAAAAC GTCTCAAAAT CGTTTCTGAG ACGTTTTAGC GTTTATTTCG TTTAGTTATC GGCATAATCG TTAAAACAGG CGTTATCGTA GCGTAAAAGC CCTTGAGCGT AGCGTGGCTT TGCAGCGAAG ATGTTGTCTG TTAGATTATG AAAGCCGATG ACTGAATGAA ATAATAAGCG CAGCGCCCTT CTATTTCGGT TGGAGGAGGC TCAAGGGAGT ATGAGGGAAT GAAATTCCCT CATGGGTTTG ATTTTAAAAA TTGCTTGCAA TTTTGCCGAG CGGTAGCGCT GGAAAATTTT TGAAAAAAAT TTGGAATTTG GAAAAAAATG GGGGGAAAGG AAGCGAATTT TGCTTCCGTA CTACGACCCC CCATTAAGTG CCGAGTGCCA ATTTTTGTGC CAAAAACGCT CTATCCCAAC TGGCTCAAGG GTTTAAGGGG TTTTTCAATC GCCAACGAAT CGCCAACGTT TTCGCCAACG TTTTTTATAA ATCTATATTT AAGTAGCTTT ATTGTTGTTT TTATGATTAC AAAGTGATAC ACTAACTTTA TAAAATTATT TGATTGGAGT TTTTTAAATG GTGATTTCAG AATCGAAAAA AAGAGTTATG ATTTCTCTGA CAAAAGAGCA AGATAAAAAA TTAACAGATA TGGCGAAACA AAAAGGTTTT TCAAAATCTG CGGTTGCGGC GTTAGCTATA GAAGAATATG CAAGAAAGGA ATCAGAACAA AAAAAATAAG CGAAAGCTCG CGTTTTTAGA AGGATACGAG TTTTCGCTAC TTGTTTTTGA TAAGGTAATT ATATCATGGC TATTAAAAAT ACTAAAGCTA GAAATTTTGG ATTTTTATTA TATCCTGACT CAATTCCTAA TGATTGGAAA GAAAAATTAG AGAGTTTGGG CGTATCTATG GCTGTCAGTC CTTTACACGA TATGGACGAA AAAAAAGATA AAGATACATG GAATAATAGT AATATTATAC AAAATGGAAA GCACTATAAA AAACCACACT ATCACGTTAT ATATATTGCA CGAAATCCTG TAACAATAGA AAGCGTTAGG AACAAGATTA AGCGAAAATT GGGGAATAGT TCAGTTGCTC ATGTTGAGAT ACTTGATTAT ATCAAAGGTT CATATGAATA TTTGACTCAT GAATCAAAGG ACGCTATTGC TAAGAATAAA CATATATACG ACAAAAAAGA TATTTTGAAC ATTAATGATT TTGATATTGA CCGCTATATA ACACTTGATG AAAGCCAAAA AAGAGAATTG AAGAATTTAC TTTTAGATAT AGTGGATGAC TATAATTTGG TAAATACAAA AGATTTAATG GCTTTTATTC GCCTTAGGGG AGCGGAGTTT GGAATTTTAA ATACGAATGA TGTAAAAGAT ATTGTTTCAA CAAACTCTAG CGCCTTTAGA TTATGGTTTG AGGGCAATTA TCAGTGTGGA TATAGAGCAA GTTATGCAAA GGTTCTTGAT GCTGAAACGG GGGAAATAAA ATGACAAACA AAGAAAAAGA GTTATTTGCT GAAAATGAGG AATTAAAAAA AGAAATTAAG GACTTAAAAG AGCGTATTGA AAGATACAGA GAAATGGAAG TTGAATTAAG TACAACAATA GATTTATTGA GAGGAGGGAT TATTGAATAA ATAAAAGCCC CCTGACGAAA GTCGAAGGGG GTTTTTATTT TGGTTTGATG TTGCGATTAA TAGCAATACA ATTGCAATAA ACAAAATGAT CTTCCTTCAG GTTATGACCA TCTGTGCCAG TTCGTAATGT CTGGTCAACT TTCCGACTCT GAGAAACTTC TGGAATCGCT AGAGAATTTC TGGAATGGGA TTCAGGAGTG GACAGAACGA CACGGATATA TAGTGGATGT GTCAAAACGC ATACCATTTT GAACGATGAC CTCTAATAAT TGTTAATCAT GTTGGTTACG TATTTATTAA CTTCTCCTAG TATTAGTAAT TATCATGGCT GTCATGGCGC ATTAACGGAA TAAAGGGTGT GCTTAAATCG GGCCATTTTG CGTAATAAGA AAAAGGATTA ATTATGAGCG AATTGAATTA ATAATAAGGT AATAGATTTA CATTAGAAAA TGAAAGGGGA TTTTATGCGT GAGAATGTTA CAGTCTATCC CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAG CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG GGCCCCCCCT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCCTG CAGCCCGGGG GATCCACTAG TT
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