pBAD43
$99.00
In stock
SKU
PL81708
pBAD-43
100μg liquid plasmid(Instantly):$134
| name | pBAD43 | another name | apBAD43 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Spe |
| Selective marker | Fluorescent labeling | ||
| Promoter | BAD | Replicon | pSC101 |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | pBAD-F： | |
| 3′ sequencing primer | pBAD-R： | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCTGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCTGCTCAT GTTTGACAGC TTATCATCGA TGCATAATGT GCCTGTCAAA TGGACGAAGC AGGGATTCTG CAAACCCTAT GCTACTCCGT CAAGCCGTCA ATTGTCTGAT TCGTTACCAA TTATGACAAC TTGACGGCTA CATCATTCAC TTTTTCTTCA CAACCGGCAC GGAACTCGCT CGGGCTGGCC CCGGTGCATT TTTTAAATAC CCGCGAGAAA TAGAGTTGAT CGTCAAAACC AACATTGCGA CCGACGGTGG CGATAGGCAT CCGGGTGGTG CTCAAAAGCA GCTTCGCCTG GCTGATACGT TGGTCCTCGC GCCAGCTTAA GACGCTAATC CCTAACTGCT GGCGGAAAAG ATGTGACAGA CGCGACGGCG ACAAGCAAAC ATGCTGTGCG ACGCTGGCGA TATCAAAATT GCTGTCTGCC AGGTGATCGC TGATGTACTG ACAAGCCTCG CGTACCCGAT TATCCATCGG TGGATGGAGC GACTCGTTAA TCGCTTCCAT GCGCCGCAGT AACAATTGCT CAAGCAGATT TATCGCCAGC AGCTCCGAAT AGCGCCCTTC CCCTTGCCCG GCGTTAATGA TTTGCCCAAA CAGGTCGCTG AAATGCGGCT GGTGCGCTTC ATCCGGGCGA AAGAACCCCG TATTGGCAAA TATTGACGGC CAGTTAAGCC ATTCATGCCA GTAGGCGCGC GGACGAAAGT AAACCCACTG GTGATACCAT TCGCGAGCCT CCGGATGACG ACCGTAGTGA TGAATCTCTC CTGGCGGGAA CAGCAAAATA TCACCCGGTC GGCAAACAAA TTCTCGTCCC TGATTTTTCA CCACCCCCTG ACCGCGAATG GTGAGATTGA GAATATAACC TTTCATTCCC AGCGGTCGGT CGATAAAAAA ATCGAGATAA CCGTTGGCCT CAATCGGCGT TAAACCCGCC ACCAGATGGG CATTAAACGA GTATCCCGGC AGCAGGGGAT CATTTTGCGC TTCAGCCATA CTTTTCATAC TCCCGCCATT CAGAGAAGAA ACCAATTGTC CATATTGCAT CAGACATTGC CGTCACTGCG TCTTTTACTG GCTCTTCTCG CTAACCAAAC CGGTAACCCC GCTTATTAAA AGCATTCTGT AACAAAGCGG GACCAAAGCC ATGACAAAAA CGCGTAACAA AAGTGTCTAT AATCACGGCA GAAAAGTCCA CATTGATTAT TTGCACGGCG TCACACTTTG CTATGCCATA GCATTTTTAT CCATAAGATT AGCGGATCCT ACCTGACGCT TTTTATCGCA ACTCTCTACT GTTTCTCCAT ACCCGTTTTT TTGGGCTAGC AGGAGGAATT CACCATGGTA CCCGGGGATC CTCTAGAGTC GACCTGCAGG CATGCAAGCT TGGCTGTTTT GGCGGATGAG AGAAGATTTT CAGCCTGATA CAGATTAAAT CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG CGGCAGTAGC GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG CGCCGATGGT AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA TGGCCTTTTT GCGTTTCTAC AAACTCTTTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTAGCTTA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGAGCT TAGTAAAGCC CTCGCTAGAT TTTAATGCGG ATGTTGCGAT TACTTCGCCA ACTATTGCGA TAACAAGAAA AAGCCAGCCT TTCATGATAT ATCTCCCAAT TTGTGTAGGG CTTATTATGC ACGCTTAAAA ATAATAAAAG CAGACTTGAC CTGATAGTTT GGCTGTGAGC AATTATGTGC TTAGTGCATC TAACGCTTGA GTTAAGCCGC GCCGCGAAGC GGCGTCGGCT TGAACGAATT GTTAGACATT ATTTGCCGAC TACCTTGGTG ATCTCGCCTT TCACGTAGTG GACAAATTCT TCCAACTGAT CTGCGCGCGA GGCCAAGCGA TCTTCTTCTT GTCCAAGATA AGCCTGTCTA GCTTCACGTA TGACGGGCTG ATACTGGGCC GGCAGGCGCT CCATTGCCCA GTCGGCAGCG ACCTCCTTCG GCGCGATTTT GCCGGTTACT GCGCTGTACC AAATGCGGGA CAACGTAAGC ACTACATTTC GCTCATCGCC AGCCCAGTCG GGCGGCGAGT TCCATAGCGT TAAGGTTTCA TTTAGCGCCT CAAATAGATC CTGTTCAGGA ACCGGATCAA AGAGTTCCTC CGCCGCTGGA CCTACCAAGG CAACGCTATG TTCTCTTGCT TTTGTCAGCA AGATAGCCAG ATCAATGTCG ATCGTGGCTG GCTCGAAGAT ACCTGCAAGA ATGTCATTGC GCTGCCATTC TCCAAATTGC AGTTCGCGCT TAGCTGGATA ACGCCACGGA ATGATGTCGT CGTGCACAAC AATGGTGACT TCTACAGCGC GGAGAATCTC GCTCTCTCCA GGGGAAGCCG AAGTTTCCAA AAGGTCGTTG ATCAAAGCTC GCCGCGTTGT TTCATCAAGC CTTACGGTCA CCGTAACCAG CAAATCAATA TCACTGTGTG GCTTCAGGCC GCCATCCACT GCGGAGCCGT ACAAATGTAC GGCCAGCAAC GTCGGTTCGA GATGGCGCTC GATGACGCCA ACTACCTCTG ATAGTTGAGT CGATACTTCG GCGATCACCG CTTCCCTCAT GATGTTTAAC TTTGTTTTAG GGCGACTGCC CTGCTGCGTA ACATCGTTGC TGCTCCATAA CATCAAACAT CGACCCACGG CGTAACGCGC TTGCTGCTTG GATGCCCGAG GCATAGACTG TACCCCAAAA AAACAGTCAT AACAAGCCAT GAAAACCGCC ACTGCGCCGT TACCACCGCT GCGTTCGGTC AAGGTTCTGG ACCAGTTGCG TGAGCGCATA CGCTACTTGC ATTACAGCTT ACGAACCGAA CAGGCTTATG TCCACTGGGT TCGTGCCTTC ATCCGTTTCC ACGGTGTGCG TCACCCGGCA ACCTTGGGCA GCAGCGAAGT CGAGGCATTT CTGTCCTGGC TGGCGAACGA GCGCAAGGTT TCGGTCTCCA CGCATCGTCA GGCATTGGCG GCCTTGCTGT TCTTCTACGG CAAGGTGCTG TGCACGGATC TGCCCTGGCT TCAGGAGATC GGAAGACCTC GGCCGTCGCG GCGCTTGCCG GTGGTGCTGA CCCCGGATGA AGTAATTCCC ACGGGTTTTG CTGCCCGCAA ACGGGCTGTT CTGGTGTTGC TAGTTTGTTA TCAGAATCGC AGATCCGGCT TCAGGTTTGC CGGCTGAAAG CGCTATTTCT TCCAGAATTG CCATGATTTT TTCCCCACGG GAGGCGTCAC TGGCTCCCGT GTTGTCGGCA GCTTTGATTC GATAAGCAGC ATCGCCTGTT TCAGGCTGTC TATGTGTGAC TGTTGAGCTG TAACAAGTTG TCTCAGGTGT TCAATTTCAT GTTCTAGTTG CTTTGTTTTA CTGGTTTCAC CTGTTCTATT AGGTGTTACA TGCTGTTCAT CTGTTACATT GTCGATCTGT TCATGGTGAA CAGCTTTGAA TGCACCAAAA ACTCGTAAAA GCTCTGATGT ATCTATCTTT TTTACACCGT TTTCATCTGT GCATATGGAC AGTTTTCCCT TTGATATGTA ACGGTGAACA GTTGTTCTAC TTTTGTTTGT TAGTCTTGAT GCTTCACTGA TAGATACAAG AGCCATAAGA ACCTCAGATC CTTCCGTATT TAGCCAGTAT GTTCTCTAGT GTGGTTCGTT GTTTTTGCGT GAGCCATGAG AACGAACCAT TGAGATCATA CTTACTTTGC ATGTCACTCA AAAATTTTGC CTCAAAACTG GTGAGCTGAA TTTTTGCAGT TAAAGCATCG TGTAGTGTTT TTCTTAGTCC GTTATGTAGG TAGGAATCTG ATGTAATGGT TGTTGGTATT TTGTCACCAT TCATTTTTAT CTGGTTGTTC TCAAGTTCGG TTACGAGATC CATTTGTCTA TCTAGTTCAA CTTGGAAAAT CAACGTATCA GTCGGGCGGC CTCGCTTATC AACCACCAAT TTCATATTGC TGTAAGTGTT TAAATCTTTA CTTATTGGTT TCAAAACCCA TTGGTTAAGC CTTTTAAACT CATGGTAGTT ATTTTCAAGC ATTAACATGA ACTTAAATTC ATCAAGGCTA ATCTCTATAT TTGCCTTGTG AGTTTTCTTT TGTGTTAGTT CTTTTAATAA CCACTCATAA ATCCTCATAG AGTATTTGTT TTCAAAAGAC TTAACATGTT CCAGATTATA TTTTATGAAT TTTTTTAACT GGAAAAGATA AGGCAATATC TCTTCACTAA AAACTAATTC TAATTTTTCG CTTGAGAACT TGGCATAGTT TGTCCACTGG AAAATCTCAA AGCCTTTAAC CAAAGGATTC CTGATTTCCA CAGTTCTCGT CATCAGCTCT CTGGTTGCTT TAGCTAATAC ACCATAAGCA TTTTCCCTAC TGATGTTCAT CATCTGAGCG TATTGGTTAT AAGTGAACGA TACCGTCCGT TCTTTCCTTG TAGGGTTTTC AATCGTGGGG TTGAGTAGTG CCACACAGCA TAAAATTAGC TTGGTTTCAT GCTCCGTTAA GTCATAGCGA CTAATCGCTA GTTCATTTGC TTTGAAAACA ACTAATTCAG ACATACATCT CAATTGGTCT AGGTGATTTT AATCACTATA CCAATTGAGA TGGGCTAGTC AATGATAATT ACTAGTCCTT TTCCTTTGAG TTGTGGGTAT CTGTAAATTC TGCTAGACCT TTGCTGGAAA ACTTGTAAAT TCTGCTAGAC CCTCTGTAAA TTCCGCTAGA CCTTTGTGTG TTTTTTTTGT TTATATTCAA GTGGTTATAA TTTATAGAAT AAAGAAAGAA TAAAAAAAGA TAAAAAGAAT AGATCCCAGC CCTGTGTATA ACTCACTACT TTAGTCAGTT CCGCAGTATT ACAAAAGGAT GTCGCAAACG CTGTTTGCTC CTCTACAAAA CAGACCTTAA AACCCTAAAG GCTTAAGTAG CACCCTCGCA AGCTCGGGCA AATCGCTGAA TATTCCTTTT GTCTCCGACC ATCAGGCACC TGAGTCGCTG TCTTTTTCGT GACATTCAGT TCGCTGCGCT CACGGCTCTG GCAGTGAATG GGGGTAAATG GCACTACAGG CGCCTTTTAT GGATTCATGC AAGGAAACTA CCCATAATAC AAGAAAAGCC CGTCACGGGC TTCTCAGGGC GTTTTATGGC GGGTCTGCTA TGTGGTGCTA TCTGACTTTT TGCTGTTCAG CAGTTCCTGC CCTCTGATTT TCCAGTCTGA CCACTTCGGA TTATCCCGTG ACAGGTCATT CAGACTGGCT AATGCACCCA GTAAGGCAGC GGTATCATCA ACAGGCTTAC CCGTCTTACT GTCGGGAATT AATTCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAG
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
