pB7WG2
$128.00
In stock
SKU
PL81707
100μg liquid plasmid(Instantly):$149
| name | pB7WG2 | another name | apB7WG2 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | GateWay |
| Fragment species | Prokaryotic resistance | Chl | |
| Selective marker | Bar | Fluorescent labeling | |
| Promoter | Replicon | ||
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATTCGGGCAC GAACCCAGTG GACATAAGCC TCGTTCGGTT CGTAAGCTGT AATGCAAGTA GCGTAACTGC CGTCACGCAA CTGGTCCAGA ACCTTGACCG AACGCAGCGG TGGTAACGGC GCAGTGGCGG TTTTCATGGC TTCTTGTTAT GACATGTTTT TTTGGGGTAC AGTCTATGCC TCGGGCATCC AAGCAGCAAG CGCGTTACGC CGTGGGTCGA TGTTTGATGT TATGGAGCAG CAACGATGTT ACGCAGCAGG GCAGTCGCCC TAAAACAAAG TTAAACATCA TGGGGGAAGC GGTGATCGCC GAAGTATCGA CTCAACTATC AGAGGTAGTT GGCGTCATCG AGCGCCATCT CGAACCGACG TTGCTGGCCG TACATTTGTA CGGCTCCGCA GTGGATGGCG GCCTGAAGCC ACACAGTGAT ATTGATTTGC TGGTTACGGT GACCGTAAGG CTTGATGAAA CAACGCGGCG AGCTTTGATC AACGACCTTT TGGAAACTTC GGCTTCCCCT GGAGAGAGCG AGATTCTCCG CGCTGTAGAA GTCACCATTG TTGTGCACGA CGACATCATT CCGTGGCGTT ATCCAGCTAA GCGCGAACTG CAATTTGGAG AATGGCAGCG CAATGACATT CTTGCAGGTA TCTTCGAGCC AGCCACGATC GACATTGATC TGGCTATCTT GCTGACAAAA GCAAGAGAAC ATAGCGTTGC CTTGGTAGGT CCAGCGGCGG AGGAACTCTT TGATCCGGTT CCTGAACAGG ATCTATTTGA GGCGCTAAAT GAAACCTTAA CGCTATGGAA CTCGCCGCCC GACTGGGCTG GCGATGAGCG AAATGTAGTG CTTACGTTGT CCCGCATTTG GTACAGCGCA GTAACCGGCA AAATCGCGCC GAAGGATGTC GCTGCCGACT GGGCAATGGA GCGCCTGCCG GCCCAGTATC AGCCCGTCAT ACTTGAAGCT AGACAGGCTT ATCTTGGACA AGAAGAAGAT CGCTTGGCCT CGCGCGCAGA TCAGTTGGAA GAATTTGTCC ACTACGTGAA AGGCGAGATC ACCAAGGTAG TCGGCAAATA ATGTCTAGCT AGAAATTCGT TCAAGCCGAC GCCGCTTCGC CGGCGTTAAC TCAAGCGATT AGATGCACTA AGCACATAAT TGCTCACAGC CAAACTATCA GGTCAAGTCT GCTTTTATTA TTTTTAAGCG TGCATAATAA GCCCTACACA AATTGGGAGA TATATCATGC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG GGTGCCTTGA TGTGGGCGCC GGCGGTCGAG TGGCGACGGC GCGGCTTGTC CGCGCCCTGG TAGATTGCCT GGCCGTAGGC CAGCCATTTT TGAGCGGCCA GCGGCCGCGA TAGGCCGACG CGAAGCGGCG GGGCGTAGGG AGCGCAGCGA CCGAAGGGTA GGCGCTTTTT GCAGCTCTTC GGCTGTGCGC TGGCCAGACA GTTATGCACA GGCCAGGCGG GTTTTAAGAG TTTTAATAAG TTTTAAAGAG TTTTAGGCGG AAAAATCGCC TTTTTTCTCT TTTATATCAG TCACTTACAT GTGTGACCGG TTCCCAATGT ACGGCTTTGG GTTCCCAATG TACGGGTTCC GGTTCCCAAT GTACGGCTTT GGGTTCCCAA TGTACGTGCT ATCCACAGGA AAGAGACCTT TTCGACCTTT TTCCCCTGCT AGGGCAATTT GCCCTAGCAT CTGCTCCGTA CATTAGGAAC CGGCGGATGC TTCGCCCTCG ATCAGGTTGC GGTAGCGCAT GACTAGGATC GGGCCAGCCT GCCCCGCCTC CTCCTTCAAA TCGTACTCCG GCAGGTCATT TGACCCGATC AGCTTGCGCA CGGTGAAACA GAACTTCTTG AACTCTCCGG CGCTGCCACT GCGTTCGTAG ATCGTCTTGA ACAACCATCT GGCTTCTGCC TTGCCTGCGG CGCGGCGTGC CAGGCGGTAG AGAAAACGGC CGATGCCGGG ATCGATCAAA AAGTAATCGG GGTGAACCGT CAGCACGTCC GGGTTCTTGC CTTCTGTGAT CTCGCGGTAC ATCCAATCAG CTAGCTCGAT CTCGATGTAC TCCGGCCGCC CGGTTTCGCT CTTTACGATC TTGTAGCGGC TAATCAAGGC TTCACCCTCG GATACCGTCA CCAGGCGGCC GTTCTTGGCC TTCTTCGTAC GCTGCATGGC AACGTGCGTG GTGTTTAACC GAATGCAGGT TTCTACCAGG TCGTCTTTCT GCTTTCCGCC ATCGGCTCGC CGGCAGAACT TGAGTACGTC CGCAACGTGT GGACGGAACA CGCGGCCGGG CTTGTCTCCC TTCCCTTCCC GGTATCGGTT CATGGATTCG GTTAGATGGG AAACCGCCAT CAGTACCAGG TCGTAATCCC ACACACTGGC CATGCCGGCC GGCCCTGCGG AAACCTCTAC GTGCCCGTCT GGAAGCTCGT AGCGGATCAC CTCGCCAGCT CGTCGGTCAC GCTTCGACAG ACGGAAAACG GCCACGTCCA TGATGCTGCG ACTATCGCGG GTGCCCACGT CATAGAGCAT CGGAACGAAA AAATCTGGTT GCTCGTCGCC CTTGGGCGGC TTCCTAATCG ACGGCGCACC GGCTGCCGGC GGTTGCCGGG ATTCTTTGCG GATTCGATCA GCGGCCGCTT GCCACGATTC ACCGGGGCGT GCTTCTGCCT CGATGCGTTG CCGCTGGGCG GCCTGCGCGG CCTTCAACTT CTCCACCAGG TCATCACCCA GCGCCGCGCC GATTTGTACC GGGCCGGATG GTTTGCGACC GTCACGCCGA TTCCTCGGGC TTGGGGGTTC CAGTGCCATT GCAGGGCCGG CAGACAACCC AGCCGCTTAC GCCTGGCCAA CCGCCCGTTC CTCCACACAT GGGGCATTCC ACGGCGTCGG TGCCTGGTTG TTCTTGATTT TCCATGCCGC CTCCTTTAGC CGCTAAAATT CATCTACTCA TTTATTCATT TGCTCATTTA CTCTGGTAGC TGCGCGATGT ATTCAGATAG CAGCTCGGTA ATGGTCTTGC CTTGGCGTAC CGCGTACATC TTCAGCTTGG TGTGATCCTC CGCCGGCAAC TGAAAGTTGA CCCGCTTCAT GGCTGGCGTG TCTGCCAGGC TGGCCAACGT TGCAGCCTTG CTGCTGCGTG CGCTCGGACG GCCGGCACTT AGCGTGTTTG TGCTTTTGCT CATTTTCTCT TTACCTCATT AACTCAAATG AGTTTTGATT TAATTTCAGC GGCCAGCGCC TGGACCTCGC GGGCAGCGTC GCCCTCGGGT TCTGATTCAA GAACGGTTGT GCCGGCGGCG GCAGTGCCTG GGTAGCTCAC GCGCTGCGTG ATACGGGACT CAAGAATGGG CAGCTCGTAC CCGGCCAGCG CCTCGGCAAC CTCACCGCCG ATGCGCGTGC CTTTGATCGC CCGCGACACG ACAAAGGCCG CTTGTAGCCT TCCATCCGTG ACCTCAATGC GCTGCTTAAC CAGCTCCACC AGGTCGGCGG TGGCCCATAT GTCGTAAGGG CTTGGCTGCA CCGGAATCAG CACGAAGTCG GCTGCCTTGA TCGCGGACAC AGCCAAGTCC GCCGCCTGGG GCGCTCCGTC GATCACTACG AAGTCGCGCC GGCCGATGGC CTTCACGTCG CGGTCAATCG TCGGGCGGTC GATGCCGACA ACGGTTAGCG GTTGATCTTC CCGCACGGCC GCCCAATCGC GGGCACTGCC CTGGGGATCG GAATCGACTA ACAGAACATC GGCCCCGGCG AGTTGCAGGG CGCGGGCTAG ATGGGTTGCG ATGGTCGTCT TGCCTGACCC GCCTTTCTGG TTAAGTACAG CGATAACCTT CATGCGTTCC CCTTGCGTAT TTGTTTATTT ACTCATCGCA TCATATACGC AGCGACCGCA TGACGCAAGC TGTTTTACTC AAATACACAT CACCTTTTTA GACGGCGGCG CTCGGTTTCT TCAGCGGCCA AGCTGGCCGG CCAGGCCGCC AGCTTGGCAT CAGACAAACC GGCCAGGATT TCATGCAGCC GCACGGTTGA GACGTGCGCG GGCGGCTCGA ACACGTACCC GGCCGCGATC ATCTCCGCCT CGATCTCTTC GGTAATGAAA AACGGTTCGT CCTGGCCGTC CTGGTGCGGT TTCATGCTTG TTCCTCTTGG CGTTCATTCT CGGCGGCCGC CAGGGCGTCG GCCTCGGTCA ATGCGTCCTC ACGGAAGGCA CCGCGCCGCC TGGCCTCGGT GGGCGTCACT TCCTCGCTGC GCTCAAGTGC GCGGTACAGG GTCGAGCGAT GCACGCCAAG CAGTGCAGCC GCCTCTTTCA CGGTGCGGCC TTCCTGGTCG ATCAGCTCGC GGGCGTGCGC GATCTGTGCC GGGGTGAGGG TAGGGCGGGG GCCAAACTTC ACGCCTCGGG CCTTGGCGGC CTCGCGCCCG CTCCGGGTGC GGTCGATGAT TAGGGAACGC TCGAACTCGG CAATGCCGGC GAACACGGTC AACACCATGC GGCCGGCCGG CGTGGTGGTG TCGGCCCACG GCTCTGCCAG GCTACGCAGG CCCGCGCCGG CCTCCTGGAT GCGCTCGGCA ATGTCCAGTA GGTCGCGGGT GCTGCGGGCC AGGCGGTCTA GCCTGGTCAC TGTCACAACG TCGCCAGGGC GTAGGTGGTC AAGCATCCTG GCCAGCTCCG GGCGGTCGCG CCTGGTGCCG GTGATCTTCT CGGAAAACAG CTTGGTGCAG CCGGCCGCGT GCAGTTCGGC CCGTTGGTTG GTCAAGTCCT GGTCGTCGGT GCTGACGCGG GCATAGCCCA GCAGGCCAGC GGCGGCGCTC TTGTTCATGG CGTAATGTCT CCGGTTCTAG TCGCAAGTAT TCTACTTTAT GCGACTAAAA CACGCGACAA GAAAACGCCA GGAAAAGGGC AGGGCGGCAG CCTGTCGCGT AACTTAGGAC TTGTGCGACA TGTCGTTTTC AGAAGACGGC TGCACTGAAC GTCAGAAGCC GACTGCACTA TAGCAGCGGA GGGGTTGGAT CAAAGTACTT TGATCCCGAG GGGAACCCTG TGGTTGGCAT GCACATACAA ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGTTATTCT AATAAACGCT CTTTTCTCTT AGGTTTACCC GCCAATATAT CCTGTCAAAC ACTGATAGTT TAAACTGAAG GCGGGAAACG ACAATCTGAT CCAAGCTCAA GCTAAGCTTG AGCTCTCCCA TATGGTCGAG ATnTCCTTTG CCCCGGAGAT CACCATGGAC GACTTTCTCT ATCTCTACGA TCTAGGAAGA AAGTTCGACG GAGAAGGTGA CGATACCATG TTCACCACCG ATAATGAGAA GATTAGCCTC TTCAATTTCA GAAAGAATGC TGACCCACAG ATGGTTAGAG AGGCCTACGC GGCAGGTCTC ATCAAGACGA TCTACCCGAG TAATAATCTC CAGGAGATCA AATACCTTCC CAAGAAGGTT AAAGATGCAG TCAAAAGATT CAGGACTAAC TGCATCAAGA ACACAGAGAA AGATATATTT CTCAAGATCA GAAGTACTAT TCCAGTATGG ACGATTCAAG GCTTGCTTCA TAAACCAAGG CAAGTAATAG AGATTGGAGT CTCTAAGAAA GTAGTTCCTA CTGAATCAAA GGCCATGGAG TCAAAAATTC AGATCGAGGA TCTAACAGAA CTCGCCGTGA AGACTGGCGA ACAGTTCATA CAGAGTCTTT TACGACTCAA TGACAAGAAG AAAATCTTCG TCAACATGGT GGAGCACGAC ACTCTCGTCT ACTCCAAGAA TATCAAAGAT ACAGTCTCAG AAGACCAAAG GGCTATTGAG ACTTTTCAAC AAAGGGTAAT ATCGGGAAAC CTCCTCGGAT TCCATTGCCC AGCTATCTGT CACTTCATCA AAAGGACAGT AGAAAAGGAA GGTGGCACCT ACAAATGCCA TCATTGCGAT AAAGGAAAGG CTATCGTTCA AGATGCCTCT GCCGACAGTG GTCCCAAAGA TGGACCCCCA CCCACGAGGA GCATCGTGGA AAAAGAAGAC GTTCCAACCA CGTCTTCAAA GCAAGTGGAT TGATGTGATA TCTCCACTGA CGTAAGGGAT GACGCACAAT CCCACTATCC TTCGCAAGAC CCTTCCTCTA TATAAGGAAG TTCATTTCAT TTGGAGAGGA CTGCAGGACG ATCCGTATTT TTACAACAAT TACCACAACA AAACAAACAA CAAACAACAT TACAATTTAC TATTCTAGTC GACCTGCAGG CGGCCGCACT AGTGATATCA CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC CGGCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA GGGTTTATTG AGAATATGTT TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG TGGCCAATAT GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT TATACGCAAG GCGACAAGGT GCTGATGCCG CTGGCGATTC AGGTTCATCA TGCCGTCTGT GATGGCTTCC ATGTCGGCAG AATGCTTAAT GAATTACAAC AGTACTGCGA TGAGTGGCAG GGCGGGGCGT AAACGCGTGG ATCCGGCTTA CTAAAAGCCA GATAACAGTA TGCGTATTTG CGCGCTGATT TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA AGAGGTGTGC TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC TCAAGGCATA TATGATGTCA ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC CCGTCGTCTG CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC CCGGTTTATT GAAATGAACG GCTCTTTTGC TGACGAGAAC AGGGACTGGT GAAATGCAGT TTAAGGTTTA CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG ATATTATTGA CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT CAGATAAAGT CTCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA TGATGACCAC CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGTGATATC CCGCGGCCAT GCTAGAGTCC GCAAAAATCA CCAGTCTCTC TCTACAAATC TATCTCTCTC TATTTTTCTC CAGAATAATG TGTGAGTAGT TCCCAGATAA GGGAATTAGG GTTCTTATAG GGTTTCGCTC ATGTGTTGAG CATATAAGAA ACCCTTAGTA TGTATTTGTA TTTGTAAAAT ACTTCTATCA ATAAAATTTC TAATTCCTAA AACCAAAATC CAGTGACCTG CAGGCATGCG ACGTCGGGCC CTCTAGAGGA TCCCCGGGTA CCGCGAATTA TCATACATGA GAATTAAGGG AGTCACGTTA TGACCCCCGC CGATGACGCG GGACAAGCCG TTTTACGTTT GGAACTGACA GAACCGCAAC GTTGAAGGAG CCACTCAGCC GGGTTTCTGG AGTTTAATGA GCTAAGCACA TACGTCAGAA ACCATTATTG CGCGTTCAAA AGTCGCCTAA GGTCACTATC AGCTAGCAAA TATTTCTTGT CAAAAATGCT CCACTGACGT TCCATAAATT CCCCTCGGTA TCCAATTAGA GTCTCATATT CACTCTCAAC TCGATCGAGG GGGATCTACC ATGAGCCCAG AACGACGCCC GGCCGACATC CGCCGTGCCA CCGAGGCGGA CATGCCGGCG GTCTGCACCA TCGTCAACCA CTACATCCAG ACAAGCACGG TCAACTTCCG TACCGAGCCG CAGGAACCGC AGGAGTGGAC GGACGACCTC GTCCGTCTGC GGGAGCGCTA TCCCTGGCTC GTCGCCGAGG TGGACGGCGA GGTCGCCGGC ATCGCCTACG CGGGTCCCTG GAAGGCACGC AACGCCTACG ACTGGACGGC CGAGTCGACC GTGTACGTCT CCCCCCGCCA CCAGCGGACG GGACTGGGCT CCACGCTCTA CACCCACCTG CTGAAGTCCC TGGAGGCACA GGGCTTCAAG AGCGTGGTCG CTGTCATCGG GCTGCCCAAC GACCCGAGCG TGCGCATGCA CGAGGCGCTC GGATATGCCC CCCGCGGCAT GCTGCGGGCG GCCGGCTTCA AGCACGGGAA CTGGCATGAC GTGGGTTTCT GGCAGCTGGA CTTCAGCCTG CCGGTGCCGC CCCGTCCGGT CCTGCCCGTC ACCGAAATCT GATGACCCCT AGAGTCAAGC AGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTTG ATTAGAGTCC CGCAATTATA CATTTAATAC GCGATAGAAA ACAAAATATA GCGCGCAAAC TAGGATAAAT TATCGCGCGC GGTGTCATCT ATGTTACTAG ATCGACCGGC ATGCAAGCTG ATAATTCAAT TCGGCGTTAA TTCAGTACAT TAAAAACGTC CGCAATGTGT TATTAAGTTG TCTAAGCGTC AATTTGTTTA CACCACAATA TATCCTGCCA CCAGCCAGCC AACAGCTCCC CGACCGGCAG CTCGGCACAA AATCACCACT CGATACAGGC AGCCCATCAG TCCGGGACGG CGTCAGCGGG AGAGCCGTTG TAAGGCGGCA GACTTTGCTC ATGTTACCGA TGCTATTCGG AAGAACGGCA ACTAAGCTGC CGGGTTTGAA ACACGGATGA TCTCGCGGAG GGTAGCATGT TGATTGTAAC GATGACAGAG CGTTGCTGCC TGTGATCA
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