pAX01-ComK
$128.00
In stock
SKU
PL81691
整合型木糖诱导
100μg liquid plasmid(Instantly):$149
| name | pAX01-ComK | another name | apAX01-ComK |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Bacillus subtilis | Prokaryotic resistance | Amp |
| Selective marker | Ery | Fluorescent labeling | |
| Promoter | xylA | Replicon | repB |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | rrnBT1-R：gagcgttcaccgacaaacaac | |
| 3′ sequencing primer | pAX01-R：tttatccaccgaactaagttgg | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGGGTGATGT CAAAGCTTGA AAAAACGCAC GTAACAAAAG CAAAATTTAT GCTCCATGGG GGAGACTACA ACCCCGATCA GTGGCTGGAT CGGCCCGATA TTTTAGCTGA CGATATCAAA CTGATGAAGC TTTCTCATAC GAATACGTTT TCTGTCGGCA TTTTTGCATG GAGCGCACTT GAGCCGGAGG AGGGCGTATA TCAATTTGAA TGGCTGGATG ATATTTTTGA GCGGATTCAC AGTATAGGCG GCCGGGTCAT ATTAGCAACG CCGAGCGGAG CCCGTCCGGC CTGGCTGTCG CAAACCTATC CGGAAGTTTT GCGCGTCAAT GCCTCCCGCG TCAAACAGCT GCACGGCGGA AGGCACAACC ACTGCCTCAC ATCTAAAGTC TACCGAGAAA AAACACGGCA CATCAACCGC TTATTAGCAG AACGATACGG ACATCACCCG GCGCTGTTAA TGTGGCACAT TTCAAACGAA TACGGGGGAG ATTGCCACTG TGAATCGATG CGGCCGCTCT AGAGCAACGT TCTTGCCATT GCTGCATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA TTAATTAATC ATCGGGAAGA TCTTCATCAC CGAAACGCGG CAGGCAGCTC TAGAGTTAAC AAGAGTTTGT AGAAACGCAA AAAGGCCATC CGTCAGGATG GCCTTCTGCT TAGCTAGAGC GGCGGATTTG TCCTACTCAG GAGAGCGTTC ACCGACAAAC AACAGATAAA ACGAAAGGCC CAGTCTTTCG ACTGAGCCTT TCGTTTTATT TGATGCCTCA AGCTAGAGAG TCATTACCAG ATCTCACTGC AGAGATCCCC GGGTCTAGAG TCTAGGGACC TCTTTAGCTC CTTGGAAGCT GTCAGTAGTA TACCTAATAA TTTATCTACA TTCCCTTTAG TAACGTGTAA CTTTCCAAAT TTACAAAAGC GACTCATAGA ATTATTTCCT CCCGTTAAAT AATAGATAAC TATTAAAAAT AGACAATACT TGCTCATAAG TAACGGTACT TAAATTGTTT ACTTTGGCGT GTTTCATTGC TTGATGAAAC TGATTTTTAG TAAACAGTTG ACGATATTCT CGATTGACCC ATTTTGAAAC AAAGTACGTA TATAGCTTCC AATATTTATC TGGAACATCT GTGGTATGGC GGGTAAGTTT TATTAAGACA CTGTTTACTT TTGGTTTAGG ATGAAAGCAT TCCGCTGGCA GCTTAAGCAA TTGCTGAATC GAGACTTGAG TGTGCAAGAG CAACCCTAGT GTTCGGTGAA TATCCAAGGT ACGCTTGTAG AATCCTTCTT CAACAATCAG ATAGATGTCA GACGCATGGC TTTCAAAAAC CACTTTTTTA ATAATTTGTG TGCTTAAATG GTAAGGAATA CTCCCAACAA TTTTATACCT CTGTTTGTTA GGGAATTGAA ACTGTAGAAT ATCTTGGTGA ATTAAAGTGA CACGAGTATT CAGTTTTAAT TTTTCTGACG ATAAGTTGAA TAGATGACTG TCTAATTCAA TAGACGTTAC CTGTTTACTT ATTTTAGCCA GTTTCGTCGT TAAATGCCCT TTACCTGTTC CAATTTCGTA AACGGTATCG GTTTCTTTTA AATTCAATTG TTTTATTATT TGGTTGAGTA CTTTTTCACT CGTTAAAAAG TTTTGAGAAT ATTTTATATT TTTGTTCATG TAATCACTCC TTCTTAATTA CAAATTTTTA GCATCTAATT TAACTTCAAT TCCTATTATA CAAAATTTTA AGATACTGCA CTATCAACAC ACTCTTAAGT TTGCTTCTAA GTCTTATTTC CATAACTTCT TTTACGTTTC CGCCATTCTT TGCTGTTTCG ATTTTTATGA TATGGTGCAA GTCAGCACGA ACACGAACCG TCTTATCTCC CATTATATCT TTTTTTGCAC TGATTGGTGT ATCATTTCGT TTTTCTTTTT GTGCTAGAGG ATCAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTGCGGCCG CCCGCGGCTA TTTTTCTAAT ACCGTTCCCC GAGCTCACGC AAAATAAAAT CATAAATCAT CTTCGTCCGC TCTTCTTTCG GGTACAGCAT AAATTCCTGT CTTTTCGGCA CGCGGTGGTC GATTCTGTCT TGGAATTTGG TTCTGAGCCA CGCTGTTCGG TATACCTGGT TCTCGAACGA ATTATAAGAG ATCGGCAGCT CCATCGTTTT CCCATTGGAA AACGTCACTT CCGTATCGTC GAATTCAGTC GCTTTGAATT CTTTTACATG CACATGGGAA ATCCAGCCGC ATTGGGGTCT TGTCGAAGAA AGTGTAGGGA ATAAAAAGAT TTGGTTCGAA GGGTCCACCA TGATCGGCGG CTTGTGTGAA ATTTTTGTCA CTTCATAAGT TCCTGCTTTT CTTCCCGCAT AGCTTGATCC AAAAAATCGG CAGCTTCTGT CGACAATTTG CAGCGGCTTC ATGTTTACAT AAAACACGCA ATCTTTTTCA ATAATTTTGG AACAGATTTT GCCGTCTATT TCTTCTGGCA GCACGGCAAT TGTTGCGCCG TTCACTTCAT ACGATTCTAA AGGTGCGTCT GTTTTCTGAC TCATATTATG GCCTCCTTTG GATCCCATTT CCCCCTTTGA TTTTTAGATA TCACTAGTTT GGACCATTTG TCATTTCCCC CTTTGATTTA AGTGAACAAG TTTATCCATC AACTATCTTA ATTGAGTTAG TTTGTTTATC CAATAAACTA ACTTTATCTC ATCATATACA AAATAAATGT TTATTTCAAT GTTTTTTTTA GAAAATTTAG TTATAATATT AGATATGATA CTTTTAAATA TCTAATTCAA GCTTCAAAAA ACACCAACTT AGTTCGGTGG ATAAACAAAG GAGTGGTTAT TATTCAAATT GCAGATCAAG CTTTAGTAAA AAAAATGAAT CAAAAATTAA TATTAGATGA AATTTTGAAG AACTCCCCTG TCTCCAGGGC AACTCTCTCT GAGATTACAG GATTAAACAA GTCTACTGTC TCCTCTCAAG TAAATACACT GCTTGAAAAA GATTTTATTT TTGAAATTGG GGCAGGGCAA TCTAGAGGCG GCAGAAGACC TGTAATGCTT GTTTTTAATA AGAATGCAGG CTACTCGATT GGTATTGATA TAGGAGTCGA CTATCTTAAC GGAATTCTAA CCGACTTAGA AGGAAATATT ATTCTCGAGA AGACTTCTGA CTTGTCTAGT TCTTCCGCTA GTGAAGTAAA AGAGATTTTA TTTGCACTTA TTCATGGTTT TGTAACCCAT ATGCCTGAGT CCCCTTATGG TCTAGTCGGA ATAGGAATTT GTGTTCCAGG CCTTGTAGAT CGTCATCAGC AAATTATTTT CATGCCTAAC TTAAATTGGA ATATCAAAGA TTTGCAGTTT TTAATTGAGA GTGAGTTTAA TGTTCCGGTT TTTGTTGAAA ATGAAGCTAA TGCAGGAGCA TACGGTGAAA AAGTATTTGG TATGACAAAA AACTATGAAA ACATCGTTTA CATCAGTATT AATATCGGAA TTGGAACTGG ACTTGTTATT AACAACGAAT TGTATAAAGG TGTTCAGGGT TTTTCTGGGG AAATGGGTCA TATGACGATA GATTTTAATG GACCCAAATG CAGCTGTGGA AATCGAGGCT GTTGGGAATT ATATGCTTCT GAAAAAGCGT TACTGGCTTC GCTCTCTAAA GAAGAAAAGA ATATTTCTCG AAAAGAGATT GTGGAACGCG CAAATAAAAA TGATGTAGAA ATGTTAAATG CACTTCAAAA CTTTGGCTTT TATATCGGAA TTGGATTAAC CAATATCCTT AATACATTTG ATATAGAAGC TGTTATCTTG AGAAATCATA TAATTGAATC TCATCCCATT GTTTTAAATA CGATTAAAAA CGAAGTTTCT TCTAGAGTCC ATTCTCATTT AGACAATAAA TGTGAACTAT TGCCTTCTTC GTTAGGAAAA AATGCACCTG CTTTAGGAGC GGTTTCTATC GTTATTGATT CTTTTTTAAG TGTTACCCCT ATAAGTTAGG AGCTCCCCGG GACGTTCTTG CCATTGCTGC ATAAAAAACG CCCGGCGGCA ACCGAGCGTT CTGAATTAAT TAATCATCGC GACTGCAGAG ATATCGATTT CAAGCTATAT TTGGAGTTGA GCCTCTTGAA ACGGACACCC TGTATCCGAA GGATCGAAAC GCTGTCAGCT ACCGCAGCCA AATATATGAA ATGAAGGATT ATGCAACCGT GATTGATGTA AAGACAGCTT CAGTGGAAGC GGTGTATCAA GAAGATTTTT ATGCGCGCAC GCCAGCGGTC ACAAGCCATG AGTATCAGCA GGGCAAGGCG TATTTTATCG GCGCGCGTTT GGAGGATCAA TTTCAGCGTG ATTTCTATGA GGGTCTGATC ACAGACCTGT CTCTCTCTCC AGTTTTTCCG GTTCGGCACG GAAAAGGCGT CTCCGTACAA GCGAGGCAGG ATCAGGACAA TGATTATATT TTTGTCATGA ATTTCACGGA AGAAAAACAG CTGGTCACGT TTGATCAGAG TGTGAAGGAC ATAATGACAG GAGACATATT GTCAGGCGAC CTGACGATGG AAAAGTATGA AGTGAGAATT GTCGTAAACA CACATTAGCC CATCATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTTGATGCC TCCGTGTAAG GGGGAATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGACTA AGAAAATGCC GTCAAATCCG CTCGCCATGA CTTCACTAAC GATGCCTTTG AAAATCTTCA AGTTCTTTTC TACTAATTCA AGGCGTGTCT CACCAGGTTT TTGGTTTGCT CCGGCGCAAA TGCAGACAAT ATCAGGAT
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