pGR107
$128.00
In stock
SKU
PL81685
VIGS
100μg liquid plasmid(Instantly):$149
| name | pGR107 | another name | apGR107 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Interfere with silence | Fragment type | shRNA |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | ||
| Promoter | 35s | Replicon | pSa |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTTTTATCCC CGGAAGCCTG TGGATAGAGG GTAGTTATCC ACGTGAAACC GCTAATGCCC CGCAAAGCCT TGATTCACGG GGCTTTCCGG CCCGCTCCAA AAACTATCCA CGTGAAATCG CTAATCAGGG TACGTGAAAT CGCTAATCGG AGTACGTGAA ATCGCTAATA AGGTCACGTG AAATCGCTAA TCAAAAAGGC ACGTGAGAAC GCTAATAGCC CTTTCAGATC AACAGCTTGC AAACACCCCT CGCTCCGGCA AGTAGTTACA GCAAGTAGTA TGTTCAATTA GCTTTTCAAT TATGAATATA TATATCAATT ATTGGTCGCC CTTGGCTTGT GGACAATGCG CTACGCGCAC CGGCTCCGCC CGTGGACAAC CGCAAGCGGT TGCCCACCGT CGAGCGCCAG CGCCTTTGCC CACAACCCGG CGGCCGGCCG CAACAGATCG TTTTATAAAT TTTTTTTTTT GAAAAAGAAA AAGCCCGAAA GGCGGCAACC TCTCGGGCTT CTGGATTTCC GATCCCCGGA ATTAGATCTT GGCAGGATAT ATTGTGGTGT AACGTTCTCG CATGCCTGCA GGTCAACATG GTGGAGCACG ACACGCTTGT CTACTCCAAA AATATCAAAG ATACAGTCTC AGAAGACCAA AGGGCAATTG AGACTTTTCA ACAAAGGGTA ATATCCGGAA ACCTCCTCGG ATTCCATTGC CCAGCTATCT GTCACTTTAT TGTGAAGATA GTGGAAAAGG AAGGTGGCTC CTACAAATGC CATCATTGCG ATAAAGGAAA GGCCATCGTT GAAGATGCCT CTGCCGACAG TGGTCCCAAA GATGGACCCC CACCCACGAG GAGCATCGTG GAAAAAGAAG ACGTTCCAAC CACGTCTTCA AAGCAAGTGG ATTGATGTGA TATCTCCACT GACGTAAGGG ATGACGCACA ATCCCACTAT CCTTCGCAAG ACCCTTCCTC TATATAAGGA AGTTCATTTC ATTTGGAGAG GAGAAAACTA AACCATACAC CACCAACACA ACCAAACCCA CCACGCCCAA TTGTTACACA CCCGCTTGAA AAAGAAAGTT TAACAAATGG CCAAGGTGCG CGAGGTTTAC CAATCTTTTA CAGACTCCAC CACAAAAACT CTCATCCAAG ATGAGGCTTA TAGAAACATT CGCCCCATCA TGGAAAAACA CAAACTAGCT AACCCTTACG CTCAAACGGT TGAAGCGGCT AATGATCTAG AGGGGTTCGG CATAGCCACC AATCCCTATA GCATTGAATT GCATACACAT GCAGCCGCTA AGACCATAGA GAATAAACTT CTAGAGGTGC TTGGTTCCAT CCTACCACAA GAACCTGTTA CATTTATGTT TCTTAAACCC AGAAAGCTAA ACTACATGAG AAGAAACCCG CGGATCAAGG ACATTTTCCA AAATGTTGCC ATTGAACCAA GAGACGTAGC CAGGTACCCC AAGGAAACAA TAATTGACAA ACTCACAGAG ATCACAACGG AAACAGCATA CATTAGTGAC ACTCTGCACT TCTTGGATCC GAGCTACATA GTGGAGACAT TCCAAAACTG CCCAAAATTG CAAACATTGT ATGCGACCTT AGTTCTCCCC GTTGAGGCAG CCTTTAAAAT GGAAAGCACT CACCCGAACA TATACAGCCT CAAATACTTC GGAGATGGTT TCCAGTATAT ACCAGGCAAC CATGGTGGCG GGGCATACCA TCATGAATTC GCTCATCTAC AATGGCTCAA AGTGGGAAAG ATCAAGTGGA GGGACCCCAA GGATAGCTTT CTCGGACATC TCAATTACAC GACTGAGCAG GTTGAGATGC ACACAGTGAC AGTACAGTTG CAGGAATCGT TCGCGGCAAA CCACTTGTAC TGCATCAGGA GAGGAGACTT GCTCACACCG GAGGTGCGCA CTTTCGGCCA ACCTGACAGG TACGTGATTC CACCACAGAT CTTCCTCCCA AAAGTTCACA ACTGCAAGAA GCCGATTCTC AAGAAAACTA TGATGCAGCT CTTCTTGTAT GTTAGGACAG TCAAGGTCGC AAAAAATTGT GACATTTTTG CCAAAGTCAG ACAATTAATT AAATCATCTG ACTTGGACAA ATACTCTGCT GTGGAACTGG TTTACTTAGT AAGCTACATG GAGTTCCTTG CCGATTTACA AGCTACCACC TGCTTCTCAG ACACACTTTC TGGTGGCTTG CTAACAAAGA CCCTTGCACC GGTGAGGGCT TGGATACAAG AGAAAAAGAT GCAGCTGTTT GGTCTTGAGG ACTACGCGAA GTTAGTCAAA GCAGTTGATT TCCACCCGGT GGATTTTTCT TTCAAAGTGG AAACTTGGGA CTTCAGATTC CACCCCTTGC AAGCGTGGAA AGCCTTCCGA CCAAGGGAAG TGTCGGATGT AGAGGAAATG GAAAGTTTGT TCTCAGATGG GGACCTGCTT GATTGCTTCA CAAGAATGCC AGCTTATGCG GTAAACGCAG AGGAAGATTT AGCTGCAATC AGGAAAACGC CCGAGATGGA TGTCGGTCAA GAAGTTAAAG AGCCTGCAGG AGACAGAAAT CAATACTCAA ACCCTGCAGA AACTTTCCTC AACAAGCTCC ACAGGAAACA CAGTAGGGAG GTGAAACACC AGGCCGCAAA GAAAGCTAAA CGCCTAGCTG AAATCCAGGA GTCAATGAGA GCTGAAGGTG ATGCCGAACC AAATGAAATA AGCGGGACGA TGGGGGCAAT ACCCAGCAAC GCCGAACTTC CTGGCACGAA TGATGCCAGA CAAGAACTCA CACTCCCAAC CACTAAACCT GTCCCTGCAA GGTGGGAAGA TGCTTCATTC ACAGATTCTA GTGTGGAAGA GGAGCAGGTT AAACTCCTTG GAAAAGAAAC CGTTGAAACA GCGACGCAAC AAGTCATCGA AGGACTTCCT TGGAAACACT GGATTCCTCA ATTAAATGCT GTTGGATTCA AGGCGCTGGA AATTCAGAGG GATAGGAGTG GAACAATGAT CATGCCCATC ACAGAAATGG TCTCCGGGCT GGAAAAAGAG GACTTCCCTG AAGGAACTCC AAAAGAGTTG GCACGAGAAT TGTTCGCTAT GAACAGAAGC CCTGCCACCA TCCCTTTGGA CCTGCTTAGA GCCAGAGACT ACGGCAGTGA TGTAAAGAAC AAGAGAATTG GTGCCATCAC AAAGACACAG GCAACGAGTT GGGGCGAATA CTTGACAGGA AAGATAGAAA GCTTAACTGA GAGGAAAGTT GCGACTTGTG TCATTCATGG AGCTGGAGGT TCTGGAAAAA GTCATGCCAT CCAGAAGGCA TTGAGAGAAA TTGGCAAGGG CTCGGACATC ACTGTAGTCC TGCCGACCAA TGAACTGCGG CTAGATTGGA GTAAGAAAGT GCCTAACACT GAGCCCTATA TGTTCAAGAC CTCTGAAAAG GCGTTAATTG GGGGAACAGG CAGCATAGTC ATCTTTGACG ATTACTCAAA ACTTCCTCCC GGTTACATAG AAGCCTTAGT CTGTTTCTAC TCTAAAATCA AGCTAATCAT TCTAACAGGA GATAGCAGAC AAAGCGTCTA CCATGAAACT GCTGAGGACG CCTCCATCAG GCATTTGGGA CCAGCAACAG AGTACTTCTC AAAATACTGC CGATACTATC TCAATGCCAC ACACCGCAAC AAGAAAGATC TTGCGAACAT GCTTGGTGTC TACAGTGAGA GAACGGGAGT CACCGAAATC AGCATGAGCG CCGAGTTCTT AGAAGGAATC CCAACTTTGG TACCCTCGGA TGAGAAGAGA AAGCTGTACA TGGGCACCGG GAGGAATGAC ACGTTCACAT ACGCTGGATG CCAGGGGCTA ACTAAGCCGA AGGTACAAAT AGTGTTGGAC CACAACACCC AAGTGTGTAG CGCGAATGTG ATGTACACGG CACTTTCTAG AGCCACCGAT AGGATTCACT TCGTGAACAC AAGTGCAAAT TCCTCTGCCT TCTGGGAAAA GTTGGACAGC ACCCCTTACC TCAAGACTTT CCTATCAGTG GTGAGAGAAC AAGCACTCAG GGAGTACGAG CCGGCAGAGG CAGAGCCAAT TCAAGAGCCT GAGCCCCAGA CACACATGTG TGTCGAGAAT GAGGAGTCCG TGCTAGAAGA GTACAAAGAG GAACTCTTGG AAAAGTTTGA CAGAGAGATC CACTCTGAAT CCCATGGTCA TTCAAACTGT GTCCAAACTG AAGACACAAC CATTCAGTTG TTTTCGCATC AACAAGCAAA AGATGAGACC CTCCTCTGGG CGACTATAGA TGCGCGGCTC AAGACCAGCA ATCAAGAAAC AAACTTCCGA GAATTCCTGA GCAAGAAGGA CATTGGGGAC GTTCTGTTTT TAAACTACCA AAAAGCTATG GGTTTACCCA AAGAGCGTAT TCCTTTTTCC CAAGAGGTCT GGGAAGCTTG TGCCCACGAA GTACAAAGCA AGTACCTCAG CAAGTCAAAG TGCAACTTGA TCAATGGGAC TGTGAGACAG AGCCCAGACT TCGATGAAAA TAAGATTATG GTATTCCTCA AGTCGCAGTG GGTCACAAAG GTGGAAAAAC TAGGTCTACC CAAGATTAAG CCAGGTCAAA CCATAGCAGC CTTTTACCAG CAGACTGTGA TGCTTTTTGG AACTATGGCT AGGTACATGC GATGGTTCAG ACAGGCTTTC CAGCCAAAAG AAGTCTTCAT AAACTGTGAG ACCACGCCAG ATGACATGTC TGCATGGGCC TTGAACAACT GGAATTTCAG CAGACCTAGC TTGGCTAATG ACTACACAGC TTTCGACCAG TCTCAGGATG GAGCCATGTT GCAATTTGAG GTGCTCAAAG CCAAACACCA CTGCATACCA GAGGAAATCA TTCAGGCATA CATAGATATT AAGACTAATG CACAGATTTT CCTAGGCACG TTATCAATTA TGCGCCTGAC TGGTGAAGGT CCCACTTTTG ATGCAAACAC TGAGTGCAAC ATAGCTTACA CCCATACAAA GTTTGACATC CCAGCCGGAA CTGCTCAAGT TTATGCAGGA GACGACTCCG CACTGGACTG TGTTCCAGAA GTGAAGCATA GTTTCCACAG GCTTGAGGAC AAATTACTCC TAAAGTCAAA GCCTGTAATC ACGCAGCAAA AGAAGGGCAG TTGGCCTGAG TTTTGTGGTT GGCTGATCAC ACCAAAAGGG GTGATGAAAG ACCCAATTAA GCTCCATGTT AGCTTAAAAT TGGCTGAAGC TAAGGGTGAA CTCAAGAAAT GTCAAGATTC CTATGAAATT GATCTGAGTT ATGCCTATGA CCACAAGGAC TCTCTGCATG ACTTGTTCGA TGAGAAACAG TGTCAGGCAC ACACACTCAC TTGCAGAACA CTAATCAAGT CAGGGAGAGG CACTGTCTCA CTTTCCCGCC TCAGAAACTT TCTTTAACCG TTAAGTTACC TTAGAGATTT GAATAAGATG GATATTCTCA TCAGTAGTTT GAAAAGTTTA GGTTATTCTA GGACTTCCAA ATCTTTAGAT TCAGGACCTT TGGTAGTACA TGCAGTAGCC GGAGCCGGTA AGTCCACAGC CCTAAGGAAG TTGATCCTCA GACACCCAAC ATTCACCGTG CATACACTCG GTGTCCCTGA CAAGGTGAGT ATCAGAACTA GAGGCATACA GAAGCCAGGA CCTATTCCTG AGGGCAACTT CGCAATCCTC GATGAGTATA CTTTGGACAA CACCACAAGG AACTCTAACC AGGCACTTTT TGCTGACCCT TATCAGGCAC CGGAGTTTAG CCTAGAGCCC CACTTCTACT TGGAAACATC ATTTCGAGTT CCGAGGAAAG TGGCAGATTT GATAGCTGGC TGTGGCTTCG ATTTCGAGAC CAACTCACCG GAAGAAGGGC ACTTAGAGAT CACTGGCATA TTCAAAGGGC CCCTACTCGG AAAGGTGATA GCCATTGATG AGGAGTCTGA GACAACACTG TCCAGGCATG GTGTTGAGTT TGTTAAGCCC TGCCAAGTGA CGGGACTTGA GTTCAAAGTA GTCACTATTG TGTCTGCCGC ACCAATAGAG GAAATTGGCC AGTCCACAGC TTTCTACAAC GCTATCACCA GGTCAAAGGG ATTGACATAT GTCCGCGCAG GGCCATAGGC TGACCGCTCC GGTCAATTCT GAAAAAGTGT ACATAGTATT AGGTCTATCA TTTGCTTTAG TTTCAATTAC CTTTCTGCTT TCTAGAAATA GCTTACCCCA CGTCGGTGAC AACATTCACA GCTTGCCACA CGGAGGAGCT TACAGAGACG GCACCAAAGC AATCTTGTAC AACTCCCCAA ATCTAGGGTC ACGAGTGAGT CTACACAACG GAAAGAACGC AGCATTTGCT GCCGTTTTGC TACTGACTTT GCTGATCTAT GGAAGTAAAT ACATATCTCA ACGCAATCAT ACTTGTGCTT GTGGTAACAA TCATAGCAGT CATTAGCACT TCCTTAGTGA GGACTGAACC TTGTGTCATC AAGATTACTG GGGAATCAAT CACAGTGTTG GCTTGCAAAC TAGATGCAGA AACCATAAGG GCCATTGCCG ATCTCAAGCC ACTCTCCGTT GAACGGTTAA GTTTCCATTG ATACTCGAAA GAGGTCAGCA CCAGCTAGCA TCGATTCCCG GGTCGACCGC CGATAAGCTT GATAGGGCCA TTGCCGATCT CAAGCCACTC TCCGTTGAAC GGTTAAGTTT CCATTGATAC TCGAAAGATG TCAGCACCAG CTAGCACAAC ACAGCCCATA GGGTCAACTA CCTCAACTAC CACAAAAACT GCAGGCGCAA CTCCTGCCAC AGCTTCAGGC CTGTTCACCA TCCCGGATGG GGATTTCTTT AGTACAGCCC GTGCCATAGT AGCCAGCAAT GCTGTCGCAA CAAATGAGGA CCTCAGCAAG ATTGAGGCTA TTTGGAAGGA CATGAAGGTG CCCACAGACA CTATGGCACA GGCTGCTTGG GACTTAGTCA GACACTGTGC TGATGTAGGA TCATCCGCTC AAACAGAAAT GATAGATACA GGTCCCTATT CCAACGGCAT CAGCAGAGCT AGACTGGCAG CAGCAATTAA AGAGGTGTGC ACACTTAGGC AATTTTGCAT GAAGTATGCT CCAGTGGTAT GGAACTGGAT GTTAACTAAC AACAGTCCAC CTGCTAACTG GCAAGCACAA GGTTTCAAGC CTGAGCACAA ATTCGCTGCA TTCGACTTCT TCAATGGAGT CACCAACCCA GCTGCCATCA TGCCCAAAGA GGGGCTCATC CGGCCACCGT CTGAAGCTGA AATGAATGCT GCCCAAACTG CTGCCTTTGT GAAGATTACA AAGGCCAGGG CACAATCCAA CGACTTTGCC AGCCTAGATG CAGCTGTCAC TCGAGGTCGT ATCACTGGAA CAACAACCGC TGAGGCTGTT GTCACTCTAC CACCACCATA ACTACGTCTA CATAACCGAC GCCTACCCCA GTTTCATAGT ATTTTCTGGT TTGATTGTAT GAATAATATA AATAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAC TAGTGGGTAC CGCGAATTTC CCCGATCGTT CAAACATTTG GCAATAAAGT TTCTTAAGAT TGAATCCTGT TGCCGGTCTT GCGATGATTA TCATATAATT TCTGTTGAAT TACGTTAAGC ATGTAATAAT TAACATGTAA TGCATGACGT TATTTATGAG ATGGGTTTTT ATGATTAGAG TCCCGCAATT ATACATTTAA TACGCGATAG AAAACAAAAT ATAGCGCGCA AACTAGGATA AATTATCGCG CGCGGTGTCA TCTATGTTAC TAGATCGAAT TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCCTGATGAG CTCCAGCTTT TGTTCCCTTT AGTGAGGGTT AATTCCGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG AAGGCCTTGA CAGGATATAT TGGCGGGTAA ACTAAGTCGC TGTATGTGTT TGTTTGAGAT CTCATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGGTTA CAACCAATTA ACCAATTCTG ATTAGAAAAA CTCATCGAGC ATCAAATGAA ACTGCAATTT ATTCATATCA GGATTATCAA TACCATATTT TTGAAAAAGC CGTTTCTGTA ATGAAGGAGA AAACTCACCG AGGCAGTTCC ATAGGATGGC AAGATCCTGG TATCGGTCTG CGATTCCGAC TCGTCCAACA TCAATACAAC CTATTAATTT CCCCTCGTCA AAAATAAGGT TATCAAGTGA GAAATCACCA TGAGTGACGA CTGAATCCGG TGAGAATGGC AAAAGTTTAT GCATTTCTTT CCAGACTTGT TCAACAGGCC AGCCATTACG CTCGTCATCA AAATCACTCG CATCAACCAA ACCGTTATTC ATTCGTGATT GCGCCTGAGC GAGACGAAAT ACGCGATCGC TGTTAAAAGG ACAATTACAA ACAGGAATCG AATGCAACCG GCGCAGGAAC ACTGCCAGCG CATCAACAAT ATTTTCACCT GAATCAGGAT ATTCTTCTAA TACCTGGAAT GCTGTTTTCC CTGGGATCGC AGTGGTGAGT AACCATGCAT CATCAGGAGT ACGGATAAAA TGCTTGATGG TCGGAAGAGG CATAAATTCC GTCAGCCAGT TTAGTCTGAC CATCTCATCT GTAACATCAT TGGCAACGCT ACCTTTGCCA TGTTTCAGAA ACAACTCTGG CGCATCGGGC TTCCCATACA ATCCATAGAT TGTCGCACCT GATTGCCCGA CATTATCGCG AGCCCATTTA TACCCATATA AATCAGCATC CATGTTGGAA TTTAATCGCG GCCTGGAGCA AGACGTTTCC CGTTGAATAT GGCTCATAAC ACCCCTTGTA TTACTGTTTA TGTAAGCAGA CAGTTTTATT GTTCATGATG ATATATTTTT ATCTTGTGCA ATGTAACATC AGAGATTTTG AGACACAACG TGGCTTTGTT GAATAAATCG AACTTTTGCT GAGTTGAAGG ATCAGATCAC GCATCTTCCC GACAACGCAG ACCGTTCCGT GGCAAAGCAA AAGTTCAAAA TCACCAACTG GTCCACCTAC AACAAAGCTC TCATCAACCG TGGCTCCCTC ACTTTCTGGC TGGATGATGG GGCGATTCAG GCGATCCCCA TCCAACAGCC CGCCGTCGAG CGGGCT
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