pH7GWIWG2(I)
$99.00
In stock
SKU
PL81570
100μg liquid plasmid(Instantly):$120
| name | pH7GWIWG2(I) | another name | apH7GWIWG2(I) |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | GateWay |
| Fragment species | Empty vector | Prokaryotic resistance | Chl |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | 35s | Replicon | pVS1 |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTAGAGGGCC CGACGTCGCA TGCCTGCAGG TCACTGGATT TTGGTTTTAG GAATTAGAAA TTTTATTGAT AGAAGTATTT TACAAATACA AATACATACT AAGGGTTTCT TATATGCTCA ACACATGAGC GAAACCCTAT AAGAACCCTA ATTCCCTTAT CTGGGAACTA CTCACACATT ATTCTGGAGA AAAATAGAGA GAGATAGATT TGTAGAGAGA GACTGGTGAT TTTTGCGGAC TCTAGCATGG CCGCGGGATA TCACAAGTTT GTACAAAAAA GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT CACTATGGCG GCCGCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATAA TGTGTGGATT TTGAGTTAGG ATCCGGCTTA CTAAAAGCCA GATAACAGTA TGCGTATTTG CGCGCTGATT TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA AGAGGTGTGC TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC TCAAGGCATA TATGATGTCA ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC CCGTCGTCTG CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC CCGGTTTATT GAAATGAACG GCTCTTTTGC TGACGAGAAC AGGGACTGGT GAAATGCAGT TTAAGGTTTA CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG ATATTATTGA CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT CAGATAAAGT CTCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA TGATGACCAC CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGTGATATC ACTAGTGCGG CCGCCTGCAG GTCGACCATA TGGTCGACCT GCAGGCGGCC GCACTAGTGA TGCTGTTATG TTCAGTGTCA AGCTGACCTG CAAACACGTT AAATGCTAAG AAGTTAGAAT ATATGAGACA CGTTAACTGG TATATGAATA AGCTGTAAAT AACCGAGTAT AAACTCATTA ACTAATATCA CCTCTAGAGT ATAATATAAT CAAATTCGAC AATTTGACTT TCAAGAGTAG GCTAATGTAA AATCTTTATA TATTTCTACA ATGTTCAAAG AAACAGTTGC ATCTAAACCC CTATGGCCAT CAAATTCAAT GAACGCTAAG CTGATCCGGC GAGATTTTCA GGAGCTAAGG AAGCTAAAAT GGAGAAAAAA ATCACTGGAT ATACCACCGT TGATATATCC CAATGGCATC GTAAAGAACA TTTTGAGGCA TTTCAGTCAG TTGCTCAATG TACCTATAAC CAGACCGTTC AGCTGGATAT TACGGCCTTT TTAAAGACCG TAAAGAAAAA TAAGCACAAG TTTTATCCGG CCTTTATTCA CATTCTTGCC CGCCTGATGA ATGCTCATCC GGAATTCCGT ATGGCAATGA AAGACGGTGA GCTGGTGATA TGGGATAGTG TTCACCCTTG TTACACCGTT TTCCATGAGC AAACTGAAAC GTTTTCATCG CTCTGGAGTG AATACCACGA CGATTTCCGG CAGTTTCTAC ACATATATTC GCAAGATGTG GCGTGTTACG GTGAAAACCT GGCCTATTTC CCTAAAGGGT TTATTGAGAA TATGTTTTTC GTCTCAGCCA ATCCCTGGGT GAGTTTCACC AGTTTTGATT TAAACGTGGC CAATATGGAC AACTTCTTCG CCCCCGTTTT CACCATGGGC AAATATTATA CGCAAGGCGA CAAGGTGCTG ATGCCGCTGG CGATTCAGGT TCATCATGCC GTCTGTGATG GCTTCCATGT CGGCAGAATG CTTAATGAAT TACAACAGTA CTGCGATGAG TGGCAGGGCG GGGCGTAAAC GCGTGGATCA GCTTAATATG ACTCTCAATA AAGTCTCATA CCAACAAGTG CCACCTTATT CAACCATCAA GAAAAAAGCC AAAATTTATG CTACTCTAAG GAAAACTTCA CTAAAGAAGA CGATTTAGAG TGTTTTACCA AGAATTTCTG TCATCTTACT AAACAACTAA AGATCGGTGT GATACAAAAC CTAATCTCAT TAAAGTTTAT GCTAAAATAA GCATAATTTT ACCCACTAAG CGTGACCAGA TAAACATAAC TCAGCACACC AGAGCATATA TATTGGTGGC TCAAATCATA GAAACTTACA GTGAAGACAC AGAAAGCCGT AAGAAGAGGC AAGAGTATGA AACCTTACCT CATCATTTCC ATGAGGTTGC TTCTGATCCC GCGGGATATC ACCACTTTGT ACAAGAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA TATCCAGTCA CTATGGTCGA CCTGCAGACT GGCTGTGTAT AAGGGAGCCT GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT TTCGCGGTGG CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT ATCGGTGGTC ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG GGAGACTTTA TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC GTCGCCCGGG CGTGTCAATA ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT CTCTTTTATA GGTGTAAACC TTAAACTGCA TTTCACCAGT CCCTGTTCTC GTCAGCAAAA GAGCCGTTCA TTTCAATAAA CCGGGCGACC TCAGCCATCC CTTCCTGATT TTCCGCTTTC CAGCGTTCGG CACGCAGACG ACGGGCTTCA TTCTGCATGG TTGTGCTTAC CAGACCGGAG ATATTGACAT CATATATGCC TTGAGCAACT GATAGCTGTC GCTGTCAACT GTCACTGTAA TACGCTGCTT CATAGCACAC CTCTTTTTGA CATACTTCGG GTATACATAT CAGTATATAT TCTTATACCG CAAAAATCAG CGCGCAAATA CGCATACTGT TATCTGGCTT TTAGTAAGCC GGATCCTAAC TCAAAATCCA CACATTATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG CGGCCGCCAT AGTGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT GTTTTTTATG CAAAATCTAA TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT CAGCTTTTTT GTACAAACTT GTGATATCAC TAGTGCGGCC GCCTGCAGGT CGACTAGAAT AGTAAATTGT AATGTTGTTT GTTGTTTGTT TTGTTGTGGT AATTGTTGTA AAAATACGGA TCGTCCTGCA GTCCTCTCCA AATGAAATGA ACTTCCTTAT ATAGAGGAAG GGTCTTGCGA AGGATAGTGG GATTGTGCGT CATCCCTTAC GTCAGTGGAG ATATCACATC AATCCACTTG CTTTGAAGAC GTGGTTGGAA CGTCTTCTTT TTCCACGATG CTCCTCGTGG GTGGGGGTCC ATCTTTGGGA CCACTGTCGG CAGAGGCATC TTGAACGATA GCCTTTCCTT TATCGCAATG ATGGCATTTG TAGGTGCCAC CTTCCTTTTC TACTGTCCTT TTGATGAAGT GACAGATAGC TGGGCAATGG AATCCGAGGA GGTTTCCCGA TATTACCCTT TGTTGAAAAG TCTCAATAGC CCTTTGGTCT TCTGAGACTG TATCTTTGAT ATTCTTGGAG TAGACGAGAG TGTCGTGCTC CACCATGTTG ACGAAGATTT TCTTCTTGTC ATTGAGTCGT AAAAGACTCT GTATGAACTG TTCGCCAGTC TTCACGGCGA GTTCTGTTAG ATCCTCGATC TGAATTTTTG ACTCCATGGC CTTTGATTCA GTAGGAACTA CTTTCTTAGA GACTCCAATC TCTATTACTT GCCTTGGTTT ATGAAGCAAG CCTTGAATCG TCCATACTGG AATAGTACTT CTGATCTTGA GAAATATATC TTTCTCTGTG TTCTTGATGC AGTTAGTCCT GAATCTTTTG ACTGCATCTT TAACCTTCTT GGGAAGGTAT TTGATCTCCT GGAGATTATT ACTCGGGTAG ATCGTCTTGA TGAGACCTGC CGCGTAGGCC TCTCTAACCA TCTGTGGGTC AGCATTCTTT CTGAAATTGA AGAGGCTAAT CTTCTCATTA TCGGTGGTGA ACATGGTATC GTCACCTTCT CCGTCGAACT TTCTTCCTAG ATCGTAGAGA TAGAGAAAGT CGTCCATGGT GATCTCCGGG GCAAAGGAGA TCAGCTTGGC TCTAGTCGAC CATATGGGAG AGCTCAAGCT TAGCTTGAGC TTGGATCAGA TTGTCGTTTC CCGCCTTCAG TTTAAACTAT CAGTGTTTGA CAGGATATAT TGGCGGGTAA ACCTAAGAGA AAAGAGCGTT TATTAGAATA ACGGATATTT AAAAGGGCGT GAAAAGGTTT ATCCGTTCGT CCATTTGTAT GTGCATGCCA ACCACAGGGT TCCCCTCGGG ATCAAAGTAC TTTGATCCAA CCCCTCCGCT GCTATAGTGC AGTCGGCTTC TGACGTTCAG TGCAGCCGTC TTCTGAAAAC GACATGTCGC ACAAGTCCTA AGTTACGCGA CAGGCTGCCG CCCTGCCCTT TTCCTGGCGT TTTCTTGTCG CGTGTTTTAG TCGCATAAAG TAGAATACTT GCGACTAGAA CCGGAGACAT TACGCCATGA ACAAGAGCGC CGCCGCTGGC CTGCTGGGCT ATGCCCGCGT CAGCACCGAC GACCAGGACT TGACCAACCA ACGGGCCGAA CTGCACGCGG CCGGCTGCAC CAAGCTGTTT TCCGAGAAGA TCACCGGCAC CAGGCGCGAC CGCCCGGAGC TGGCCAGGAT GCTTGACCAC CTACGCCCTG GCGACGTTGT GACAGTGACC AGGCTAGACC GCCTGGCCCG CAGCACCCGC GACCTACTGG ACATTGCCGA GCGCATCCAG GAGGCCGGCG CGGGCCTGCG TAGCCTGGCA GAGCCGTGGG CCGACACCAC CACGCCGGCC GGCCGCATGG TGTTGACCGT GTTCGCCGGC ATTGCCGAGT TCGAGCGTTC CCTAATCATC GACCGCACCC GGAGCGGGCG CGAGGCCGCC AAGGCCCGAG GCGTGAAGTT TGGCCCCCGC CCTACCCTCA CCCCGGCACA GATCGCGCAC GCCCGCGAGC TGATCGACCA GGAAGGCCGC ACCGTGAAAG AGGCGGCTGC ACTGCTTGGC GTGCATCGCT CGACCCTGTA CCGCGCACTT GAGCGCAGCG AGGAAGTGAC GCCCACCGAG GCCAGGCGGC GCGGTGCCTT CCGTGAGGAC GCATTGACCG AGGCCGACGC CCTGGCGGCC GCCGAGAATG AACGCCAAGA GGAACAAGCA TGAAACCGCA CCAGGACGGC CAGGACGAAC CGTTTTTCAT TACCGAAGAG ATCGAGGCGG AGATGATCGC GGCCGGGTAC GTGTTCGAGC CGCCCGCGCA CGTCTCAACC GTGCGGCTGC ATGAAATCCT GGCCGGTTTG TCTGATGCCA AGCTGGCGGC CTGGCCGGCC AGCTTGGCCG CTGAAGAAAC CGAGCGCCGC CGTCTAAAAA GGTGATGTGT ATTTGAGTAA AACAGCTTGC GTCATGCGGT CGCTGCGTAT ATGATGCGAT GAGTAAATAA ACAAATACGC AAGGGGAACG CATGAAGGTT ATCGCTGTAC TTAACCAGAA AGGCGGGTCA GGCAAGACGA CCATCGCAAC CCATCTAGCC CGCGCCCTGC AACTCGCCGG GGCCGATGTT CTGTTAGTCG ATTCCGATCC CCAGGGCAGT GCCCGCGATT GGGCGGCCGT GCGGGAAGAT CAACCGCTAA CCGTTGTCGG CATCGACCGC CCGACGATTG ACCGCGACGT GAAGGCCATC GGCCGGCGCG ACTTCGTAGT GATCGACGGA GCGCCCCAGG CGGCGGACTT GGCTGTGTCC GCGATCAAGG CAGCCGACTT CGTGCTGATT CCGGTGCAGC CAAGCCCTTA CGACATATGG GCCACCGCCG ACCTGGTGGA GCTGGTTAAG CAGCGCATTG AGGTCACGGA TGGAAGGCTA CAAGCGGCCT TTGTCGTGTC GCGGGCGATC AAAGGCACGC GCATCGGCGG TGAGGTTGCC GAGGCGCTGG CCGGGTACGA GCTGCCCATT CTTGAGTCCC GTATCACGCA GCGCGTGAGC TACCCAGGCA CTGCCGCCGC CGGCACAACC GTTCTTGAAT CAGAACCCGA GGGCGACGCT GCCCGCGAGG TCCAGGCGCT GGCCGCTGAA ATTAAATCAA AACTCATTTG AGTTAATGAG GTAAAGAGAA AATGAGCAAA AGCACAAACA CGCTAAGTGC CGGCCGTCCG AGCGCACGCA GCAGCAAGGC TGCAACGTTG GCCAGCCTGG CAGACACGCC AGCCATGAAG CGGGTCAACT TTCAGTTGCC GGCGGAGGAT CACACCAAGC TGAAGATGTA CGCGGTACGC CAAGGCAAGA CCATTACCGA GCTGCTATCT GAATACATCG CGCAGCTACC AGAGTAAATG AGCAAATGAA TAAATGAGTA GATGAATTTT AGCGGCTAAA GGAGGCGGCA TGGAAAATCA AGAACAACCA GGCACCGACG CCGTGGAATG CCCCATGTGT GGAGGAACGG GCGGTTGGCC AGGCGTAAGC GGCTGGGTTG TCTGCCGGCC CTGCAATGGC ACTGGAACCC CCAAGCCCGA GGAATCGGCG TGACGGTCGC AAACCATCCG GCCCGGTACA AATCGGCGCG GCGCTGGGTG ATGACCTGGT GGAGAAGTTG AAGGCCGCGC AGGCCGCCCA GCGGCAACGC ATCGAGGCAG AAGCACGCCC CGGTGAATCG TGGCAAGCGG CCGCTGATCG AATCCGCAAA GAATCCCGGC AACCGCCGGC AGCCGGTGCG CCGTCGATTA GGAAGCCGCC CAAGGGCGAC GAGCAACCAG ATTTTTTCGT TCCGATGCTC TATGACGTGG GCACCCGCGA TAGTCGCAGC ATCATGGACG TGGCCGTTTT CCGTCTGTCG AAGCGTGACC GACGAGCTGG CGAGGTGATC CGCTACGAGC TTCCAGACGG GCACGTAGAG GTTTCCGCAG GGCCGGCCGG CATGGCCAGT GTGTGGGATT ACGACCTGGT ACTGATGGCG GTTTCCCATC TAACCGAATC CATGAACCGA TACCGGGAAG GGAAGGGAGA CAAGCCCGGC CGCGTGTTCC GTCCACACGT TGCGGACGTA CTCAAGTTCT GCCGGCGAGC CGATGGCGGA AAGCAGAAAG ACGACCTGGT AGAAACCTGC ATTCGGTTAA ACACCACGCA CGTTGCCATG CAGCGTACGA AGAAGGCCAA GAACGGCCGC CTGGTGACGG TATCCGAGGG TGAAGCCTTG ATTAGCCGCT ACAAGATCGT AAAGAGCGAA ACCGGGCGGC CGGAGTACAT CGAGATCGAG CTAGCTGATT GGATGTACCG CGAGATCACA GAAGGCAAGA ACCCGGACGT GCTGACGGTT CACCCCGATT ACTTTTTGAT CGATCCCGGC ATCGGCCGTT TTCTCTACCG CCTGGCACGC CGCGCCGCAG GCAAGGCAGA AGCCAGATGG TTGTTCAAGA CGATCTACGA ACGCAGTGGC AGCGCCGGAG AGTTCAAGAA GTTCTGTTTC ACCGTGCGCA AGCTGATCGG GTCAAATGAC CTGCCGGAGT ACGATTTGAA GGAGGAGGCG GGGCAGGCTG GCCCGATCCT AGTCATGCGC TACCGCAACC TGATCGAGGG CGAAGCATCC GCCGGTTCCT AATGTACGGA GCAGATGCTA GGGCAAATTG CCCTAGCAGG GGAAAAAGGT CGAAAAGGTC TCTTTCCTGT GGATAGCACG TACATTGGGA ACCCAAAGCC GTACATTGGG AACCGGAACC CGTACATTGG GAACCCAAAG CCGTACATTG GGAACCGGTC ACACATGTAA GTGACTGATA TAAAAGAGAA AAAAGGCGAT TTTTCCGCCT AAAACTCTTT AAAACTTATT AAAACTCTTA AAACCCGCCT GGCCTGTGCA TAACTGTCTG GCCAGCGCAC AGCCGAAGAG CTGCAAAAAG CGCCTACCCT TCGGTCGCTG CGCTCCCTAC GCCCCGCCGC TTCGCGTCGG CCTATCGCGG CCGCTGGCCG CTCAAAAATG GCTGGCCTAC GGCCAGGCAA TCTACCAGGG CGCGGACAAG CCGCGCCGTC GCCACTCGAC CGCCGGCGCC CACATCAAGG CACCCTGCCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATACTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGCATGA TATATCTCCC AATTTGTGTA GGGCTTATTA TGCACGCTTA AAAATAATAA AAGCAGACTT GACCTGATAG TTTGGCTGTG AGCAATTATG TGCTTAGTGC ATCTAATCGC TTGAGTTAAC GCCGGCGAAG CGGCGTCGGC TTGAACGAAT TTCTAGCTAG ACATTATTTG CCGACTACCT TGGTGATCTC GCCTTTCACG TAGTGGACAA ATTCTTCCAA CTGATCTGCG CGCGAGGCCA AGCGATCTTC TTCTTGTCCA AGATAAGCCT GTCTAGCTTC AAGTATGACG GGCTGATACT GGGCCGGCAG GCGCTCCATT GCCCAGTCGG CAGCGACATC CTTCGGCGCG ATTTTGCCGG TTACTGCGCT GTACCAAATG CGGGACAACG TAAGCACTAC ATTTCGCTCA TCGCCAGCCC AGTCGGGCGG CGAGTTCCAT AGCGTTAAGG TTTCATTTAG CGCCTCAAAT AGATCCTGTT CAGGAACCGG ATCAAAGAGT TCCTCCGCCG CTGGACCTAC CAAGGCAACG CTATGTTCTC TTGCTTTTGT CAGCAAGATA GCCAGATCAA TGTCGATCGT GGCTGGCTCG AAGATACCTG CAAGAATGTC ATTGCGCTGC CATTCTCCAA ATTGCAGTTC GCGCTTAGCT GGATAACGCC ACGGAATGAT GTCGTCGTGC ACAACAATGG TGACTTCTAC AGCGCGGAGA ATCTCGCTCT CTCCAGGGGA AGCCGAAGTT TCCAAAAGGT CGTTGATCAA AGCTCGCCGC GTTGTTTCAT CAAGCCTTAC GGTCACCGTA ACCAGCAAAT CAATATCACT GTGTGGCTTC AGGCCGCCAT CCACTGCGGA GCCGTACAAA TGTACGGCCA GCAACGTCGG TTCGAGATGG CGCTCGATGA CGCCAACTAC CTCTGATAGT TGAGTCGATA CTTCGGCGAT CACCGCTTCC CCCATGATGT TTAACTTTGT TTTAGGGCGA CTGCCCTGCT GCGTAACATC GTTGCTGCTC CATAACATCA AACATCGACC CACGGCGTAA CGCGCTTGCT GCTTGGATGC CCGAGGCATA GACTGTACCC CAAAAAAACA TGTCATAACA AGAAGCCATG AAAACCGCCA CTGCGCCGTT ACCACCGCTG CGTTCGGTCA AGGTTCTGGA CCAGTTGCGT GACGGCAGTT ACGCTACTTG CATTACAGCT TACGAACCGA ACGAGGCTTA TGTCCACTGG GTTCGTGCCC GAATTGATCA CAGGCAGCAA CGCTCTGTCA TCGTTACAAT CAACATGCTA CCCTCCGCGA GATCATCCGT GTTTCAAACC CGGCAGCTTA GTTGCCGTTC TTCCGAATAG CATCGGTAAC ATGAGCAAAG TCTGCCGCCT TACAACGGCT CTCCCGCTGA CGCCGTCCCG GACTGATGGG CTGCCTGTAT CGAGTGGTGA TTTTGTGCCG AGCTGCCGGT CGGGGAGCTG TTGGCTGGCT GGTGGCAGGA TATATTGTGG TGTAAACAAA TTGACGCTTA GACAACTTAA TAACACATTG CGGACGTTTT TAATGTACTG AATTAACGCC GAATTGAATT ATCAGCTTGC ATGCCGGTCG ATCTAGTAAC ATAGATGACA CCGCGCGCGA TAATTTATCC TAGTTTGCGC GCTATATTTT GTTTTCTATC GCGTATTAAA TGTATAATTG CGGGACTCTA ATCATAAAAA CCCATCTCAT AAATAACGTC ATGCATTACA TGTTAATTAT TACATGCTTA ACGTAATTCA ACAGAAATTA TATGATAATC ATCGCAAGAC CGGCAACAGG ATTCAATCTT AAGAAACTTT ATTGCCAAAT GTTTGAACGA TCTGCTTGAC TCTAGGGAAT TAATTCCTGA ATCACTGCGA CCGGCCCTCC CGCGACCCAG CCGAGCGAGC TTAGCGAACT GTGGACGAGA ACTGTGCCAC CAAGCGTAAG GCCGTTCTCT CGCATTTGCC TTGCTAGGCT CGCGCGAGTT GCTGGCTGAG GCGTTCTCGA AATCAGCTCT TGTTCGGTCG GCATCTACTC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG CCCGACAGTC CCGGCTCCGG ATCGGACGAT TGCGTCGCAT CGACCCTGCG CCCAAGCTGC ATCATCGAAA TTGCCGTCAA CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT ATACGCCCGG AGCCTTGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT CTGCTGCTCC ATACAAGCCA ACCACGGCCT CCAGAAGAAG ATGTTGGCGA CCTCGTATTG GGAATCCCCG AACATCGCCT CGCTCCAGTC AATGACCGCT GTTATGCGGC CATTGTCCGT CAGGACATTG TTGGAGCCGA ATCCGCGTGC ACGAGTGCCG GACTTCGGGG CAGTCCTCGC CCAAAGCATC AGCTCATCGA GAGCCTGCGC GACGGACGCA CTGACGGTGT CGTCCATCAC AGTTTGCCAG TGATACACAT GGGGATCAGC AATCGCGCAT ATGAAATCAC GCCATGTAGT GTATTGACCG ATTCCTTGCG GTCCGAATGG GCCGAACCCG CTCGTCTGGC TAAGATCGGC CGCAGCGATA GCATCCATAG CCTCCGCGAC CGGCTGAAGA ACAGCGGGCA GTTCGGTTTC AGGCAGGTCT TGCAACGTGA CACCCTGTGC ACGGCGGGAG ATGCAATAGG TCAGGCTCTC GCTGAACTCC CCAATGTCAA GCACTTCCGG AATCGGGAGC GCGGCCGATG CAAAGTGCCG ATAAACATAA CGATCTTTGT AGAAACCATC GGCGCAGCTA TTTACCCGCA GGACATATCC ACGCCCTCCT ACATCGAAGC TGAAAGCACG AGATTCTTCG CCCTCCGAGA GCTGCATCAG GTCGGAGACG CTGTCGAACT TTTCGATCAG AAACTTCTCG ACAGACGTCG CGGTGAGTTC AGGCTTTTTC ATAGGGGGGA TTCGAGTTGA GAGTGAATAT GAGACTCTAA TTGGATACCG AGGGGAATTT ATGGAACGTC AGTGGAGCAT TTTTGACAAG AAATATTTGC TAGCTGATAG TGACCTTAGG CGACTTTTGA ACGCGCAATA ATGGTTTCTG ACGTATGTGC TTAGCTCATT AAACTCCAGA AACCCGCGGC TCAGTGGCTC CTTCAACGTT GCGGTTCTGT CAGTTCCAAA CGTAAAACGG CTTGTCCCGC GTCATCGGCG GGGTCATAAC GTGACTCCCT TAATTCTCAT GTATGATAAT TCGAGGGTAC CCGGGGATCC T
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