pK19mobSacB
$128.00
In stock
SKU
PL81550
100μg liquid plasmid(Instantly):$149
| name | pK19mobSacB | another name | apK19mobSacB |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | SacB | Fluorescent labeling | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGCCGCAAGC ACTCAGGGCG CAAGGGCTGC TAAAGGAAGC GGAACACGTA GAAAGCCAGT CCGCAGAAAC GGTGCTGACC CCGGATGAAT GTCAGCTACT GGGCTATCTG GACAAGGGAA AACGCAAGCG CAAAGAGAAA GCAGGTAGCT TGCAGTGGGC TTACATGGCG ATAGCTAGAC TGGGCGGTTT TATGGACAGC AAGCGAACCG GAATTGCCAG CTGGGGCGCC CTCTGGTAAG GTTGGGAAGC CCTGCAAAGT AAACTGGATG GCTTTCTTGC CGCCAAGGAT CTGATGGCGC AGGGGATCAA GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTCCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGGATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGCT AGAGGATCGA TCCTTTTTAA CCCATCACAT ATACCTGCCG TTCACTATTA TTTAGTGAAA TGAGATATTA TGATATTTTC TGAATTGTGA TTAAAAAGGC AACTTTATGC CCATGCAACA GAAACTATAA AAAATACAGA GAATGAAAAG AAACAGATAG ATTTTTTAGT TCTTTAGGCC CGTAGTCTGC AAATCCTTTT ATGATTTTCT ATCAAACAAA AGAGGAAAAT AGACCAGTTG CAATCCAAAC GAGAGTCTAA TAGAATGAGG TCGAAAAGTA AATCGCGCGG GTTTGTTACT GATAAAGCAG GCAAGACCTA AAATGTGTAA AGGGCAAAGT GTATACTTTG GCGTCACCCC TTACATATTT TAGGTCTTTT TTTATTGTGC GTAACTAACT TGCCATCTTC AAACAGGAGG GCTGGAAGAA GCAGACCGCT AACACAGTAC ATAAAAAAGG AGACATGAAC GATGAACATC AAAAAGTTTG CAAAACAAGC AACAGTATTA ACCTTTACTA CCGCACTGCT GGCAGGAGGC GCAACTCAAG CGTTTGCGAA AGAAACGAAC CAAAAGCCAT ATAAGGAAAC ATACGGCATT TCCCATATTA CACGCCATGA TATGCTGCAA ATCCCTGAAC AGCAAAAAAA TGAAAAATAT CAAGTTTCTG AATTTGATTC GTCCACAATT AAAAATATCT CTTCTGCAAA AGGCCTGGAC GTTTGGGACA GCTGGCCATT ACAAAACGCT GACGGCACTG TCGCAAACTA TCACGGCTAC CACATCGTCT TTGCATTAGC CGGAGATCCT AAAAATGCGG ATGACACATC GATTTACATG TTCTATCAAA AAGTCGGCGA AACTTCTATT GACAGCTGGA AAAACGCTGG CCGCGTCTTT AAAGACAGCG ACAAATTCGA TGCAAATGAT TCTATCCTAA AAGACCAAAC ACAAGAATGG TCAGGTTCAG CCACATTTAC ATCTGACGGA AAAATCCGTT TATTCTACAC TGATTTCTCC GGTAAACATT ACGGCAAACA AACACTGACA ACTGCACAAG TTAACGTATC AGCATCAGAC AGCTCTTTGA ACATCAACGG TGTAGAGGAT TATAAATCAA TCTTTGACGG TGACGGAAAA ACGTATCAAA ATGTACAGCA GTTCATCGAT GAAGGCAACT ACAGCTCAGG CGACAACCAT ACGCTGAGAG ATCCTCACTA CGTAGAAGAT AAAGGCCACA AATACTTAGT ATTTGAAGCA AACACTGGAA CTGAAGATGG CTACCAAGGC GAAGAATCTT TATTTAACAA AGCATACTAT GGCAAAAGCA CATCATTCTT CCGTCAAGAA AGTCAAAAAC TTCTGCAAAG CGATAAAAAA CGCACGGCTG AGTTAGCAAA CGGCGCTCTC GGTATGATTG AGCTAAACGA TGATTACACA CTGAAAAAAG TGATGAAACC GCTGATTGCA TCTAACACAG TAACAGATGA AATTGAACGC GCGAACGTCT TTAAAATGAA CGGCAAATGG TACCTGTTCA CTGACTCCCG CGGATCAAAA ATGACGATTG ACGGCATTAC GTCTAACGAT ATTTACATGC TTGGTTATGT TTCTAATTCT TTAACTGGCC CATACAAGCC GCTGAACAAA ACTGGCCTTG TGTTAAAAAT GGATCTTGAT CCTAACGATG TAACCTTTAC TTACTCACAC TTCGCTGTAC CTCAAGCGAA AGGAAACAAT GTCGTGATTA CAAGCTATAT GACAAACAGA GGATTCTACG CAGACAAACA ATCAACGTTT GCGCCGAGCT TCCTGCTGAA CATCAAAGGC AAGAAAACAT CTGTTGTCAA AGACAGCATC CTTGAACAAG GACAATTAAC AGTTAACAAA TAAAAACGCA AAAGAAAATG CCGATGGGTA CCGAGCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCCT CGCGGACGTG CTCATAGTCC ACGACGCCCG TGATTTTGTA GCCCTGGCCG ACGGCCAGCA GGTAGGCCGA CAGGCTCATG CCGGCCGCCG CCGCCTTTTC CTCAATCGCT CTTCGTTCGT CTGGAAGGCA GTACACCTTG ATAGGTGGGC TGCCCTTCCT GGTTGGCTTG GTTTCATCAG CCATCCGCTT GCCCTCATCT GTTACGCCGG CGGTAGCCGG CCAGCCTCGC AGAGCAGGAT TCCCGTTGAG CACCGCCAGG TGCGAATAAG GGACAGTGAA GAAGGAACAC CCGCTCGCGG GTGGGCCTAC TTCACCTATC CTGCCCGGCT GACGCCGTTG GATACACCAA GGAAAGTCTA CACGAACCCT TTGGCAAAAT CCTGTATATC GTGCGAAAAA GGATGGATAT ACCGAAAAAA TCGCTATAAT GACCCCGAAG CAGGGTTATG CAGCGGAAAA GCGCTGCTTC CCTGCTGTTT TGTGGAATAT CTACCGACTG GAAACAGGCA AATGCAGGAA ATTACTGAAC TGAGGGGACA GGCGAGAGAC GATGCCAAAG AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC AACGTCTCAT TTTCGCCAAA AGTTGGCCCA GGGCTTCCCG GTATCAACAG GGACACCAGG ATTTATTTAT TCTGCGAAGT GATCTTCCGT CACAGGTATT TATTCGGCGC AAAGTGCGTC GGGTGATGCT GCCAACTTAC TGATTTAGTG TATGATGGTG TTTTTGAGGT GCTCCAGTGG CTTCTGTTTC TATCAGCTCC TGAAAATCTC GATAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT TATGGTGAAA GTTGGAACCT CTTACGTGCC GATCAACGTC TCATTTTCGC CAAAAGTTGG CCCAGGGCTT CCCGGTATCA ACAGGGACAC CAGGATTTAT TTATTCTGCG AAGTGATCTT CCGTCACAGG TATTTATTCG GCGCAAAGTG CGTCGGGTGA TGCTGCCAAC TTACTGATTT AGTGTATGAT GGTGTTTTTG AGGTGCTCCA GTGGCTTCTG TTTCTATCAG GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACATGAT TACGCCAAGC TTGCATGCCT GCAGGTCGAC TCTAGAGGAT CCCCGGGTAC CGAGCTCGAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGATAAGCTA GCTTCACGC
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