pCXSN
$99.00
In stock
SKU
PL81524
100μg liquid plasmid(Instantly):$120
| name | pCXSN | another name | apCXSN |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | 35S | Replicon | pVS1 |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAATTCCGAT CTAGTAACAT AGATGACACC GCGCGCGATA ATTTATCCTA GTTTGCGCGC TATATTTTGT TTTCTATCGC GTATTAAATG TATAATTGCG GGACTCTAAT CATAAAAACC CATCTCATAA ATAACGTCAT GCATTACATG TTAATTATTA CATGCTTAAC GTAATTCAAC AGAAATTATA TGATAATCAT CGCAAGACCG GCAACAGGAT TCAATCTTAA GAAACTTTAT TGCCAAATGT TTGAACGATC GGGGAAATTC GCTAGTGGAT CCCCAATACT TGTATGGGGC TTACTAAAAG CCAGATAACA GTATGCGTAT TTGCGCGCTG ATTTTTGCGG TATAAGAATA TATACTGATA TGTATACCCG AAGTATGTCA AAAAGAGGTA TGCTATGAAG CAGCGTATTA CAGTGACAGT TGACAGCGAC AGCTATCAGT TGCTCAAGGC ATATATGATG TCAATATCTC CGGTCTGGTA AGCACAACCA TGCAGAATGA AGCCCGTCGT CTGCGTGCCG AACGCTGGAA AGCGGAAAAT CAGGAAGGGA TGGCTGAGGT CGCCCGGTTT ATTGAAATGA ACGGCTCTTT TGCTGACGAG AACAGGGGCT GGTGAAATGC AGTTTAAGGT TTACACCTAT AAAAGAGAGA GCCGTTATCG TCTGTTTGTG GATGTACAGA GTGATATTAT TGACACGCCC GGGCGACGGA TGGTGATCCC CCTGGCCAGT GCACGTCTGC TGTCAGATAA AGTCTCCCGT GAACTTTACC CGGTGGTGCA TATCGGGGAT GAAAGCTGGC GCATGATGAC CACCGATATG GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG ATCTCAGCCA CCGCGAAAAT GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT AAATGTCAGG CTCCCTTATA CACAGCCAGT CTGCAGGTCG ACCCATACAA GTATTGGGGA TCCCCCTCGA GTATCGTTCG TAAATGGTGA AAATTTTCAG AAAATTGCTT TTGCTTTAAA AGAAATGATT TAAATTGCTG CAATAGAAGT AGAATGCTTG ATTGCTTGAG ATTCGTTTGT TTTGTATATG TTGTGTTGAG GTCGAGGTCC TCTCCAAATG AAATGAACTT CCTTATATAG AGGAAGGGTC TTGCGAAGGA TAGTGGGATT GTGCGTCATC CCTTACGTCA GTGGAGATAT CACATCAATC CACTTGCTTT GAAGACGTGG TTGGAACGTC TTCTTTTTCC ACGATGCTCC TCGTGGGTGG GGGTCCATCT TTGGGACCAC TGTCGGCAGA GGCATCTTCA ACGATGGCCT TTCCTTTATC GCAATGATGG CATTTGTAGG AGCCACCTTC CTTTTCCACT ATCTTCACAA TAAAGTGACA GATAGCTGGG CAATGGAATC CGAGGAGGTT TCCGGATATT ACCCTTTGTT GAAAAGTCTC AATTGCCCTT TGGTCTTCTG AGACTGTATC TTTGATATTT TTGGAGTAGA CAAGTGTGTC GTGCTCCACC ATGTTATCAC ATCAATCCAC TTGCTTTGAA GACGTGGTTG GAACGTCTTC TTTTTCCACG ATGCTCCTCG TGGGTGGGGG TCCATCTTTG GGACCACTGT CGGCAGAGGC ATCTTCAACG ATGGCCTTTC CTTTATCGCA ATGATGGCAT TTGTAGGAGC CACCTTCCTT TTCCACTATC TTCACAATAA AGTGACAGAT AGCTGGGCAA TGGAATCCGA GGAGGTTTCC GGATATTACC CTTTGTTGAA AAGTCTCAAT TGCCCTTTGG TCTTCTGAGA CTGTATCTTT GATATTTTTG GAGTAGACAA GTGTGTCGTG CTCCACCATG TTGACCTGCA GGCATGCAAG CTTGGCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGCTAGAGCA GCTTGAGCTT GGATCAGATT GTCGTTTCCC GCCTTCAGTT TAAACTATCA GTGTTTGACA GGATATATTG GCGGGTAAAC CTAAGAGAAA AGAGCGTTTA TTAGAATAAC GGATATTTAA AAGGGCGTGA AAAGGTTTAT CCGTTCGTCC ATTTGTATGT GCATGCCAAC CACAGGGTTC CCCTCGGGAT CAAAGTACTT TGATCCAACC CCTCCGCTGC TATAGTGCAG TCGGCTTCTG ACGTTCAGTG CAGCCGTCTT CTGAAAACGA CATGTCGCAC AAGTCCTAAG TTACGCGACA GGCTGCCGCC CTGCCCTTTT CCTGGCGTTT TCTTGTCGCG TGTTTTAGTC GCATAAAGTA GAATACTTGC GACTAGAACC GGAGACATTA CGCCATGAAC AAGAGCGCCG CCGCTGGCCT GCTGGGCTAT GCCCGCGTCA GCACCGACGA CCAGGACTTG ACCAACCAAC GGGCCGAACT GCACGCGGCC GGCTGCACCA AGCTGTTTTC CGAGAAGATC ACCGGCACCA GGCGCGACCG CCCGGAGCTG GCCAGGATGC TTGACCACCT AGCCCTGGCG ACGTTGTGAC AGTGACCAGG CTAGACCGCC TGGCCCGCAG CACCCGCGAC CTACTGGACA TTGCCGAGCG CATCCAGGAG GCCGGCGCGG GCCTGCGTAG CCTGGCAGAG CCGTGGGCCG ACACCACCAC GCCGGCCGGC CGCATGGTGT TGACCGTGTT CGCCGGCATT GCCGAGTTCG AGCGTTCCCT AATCATCGAC CGCACCCGGA GCGGGCGCGA GGCCGCCAAG GCCCGAGGCG TGAAGTTTGG CCCCCGCCCT ACCCTCACCC CGGCACAGAT CGCGCACGCC CGCGAGCTGA TCGACCAGGA AGGCCGCACC GTGAAAGAGG CGGCTGCACT GCTTGGCGTG CATCGCTCGA CCCTGTACCG CGCACTTGAG CGCAGCGAGG AAGTGACGCC CACCGAGGCC AGGCGGCGCG GTGCCTTCCG TGAGGACGCA TTGACCGAGG CCGACGCCCT GGCGGCCGCC GAGAATGAAC GCCAAGAGGA ACAAGCATGA AACCGCACCA GGACGGCCAG GACGAACCGT TTTTCATTAC CGAAGAGATC GAGGCGGAGA TGATCGCGGC CGGGTACGTG TTCGAGCCGC CCGCGCACGT CTCAACCGTG CGGCTGCATG AAATCCTGGC CGGTTTGTCT GATGCCAAGC TGGCGGCCTG GCCGGCCAGC TTGGCCGCTG AAGAAACCGA GCGCCGCCGT CTAAAAAGGT GATGTGTATT TGAGTAAAAC AGCTTGCGTC ATGCGGTCGC TGCGTATATG ATGCGATGAG TAAATAAACA AATACGCAAG GGGAACGCAT GAAGGTTATC GCTGTACTTA ACCAGAAAGG CGGGTCAGGC AAGACGACCA TCGCAACCCA TCTAGCCCGC GCCCTGCAAC TCGCCGGGGC CGATGTTCTG TTAGTCGATT CCGATCCCCA GGGCAGTGCC CGCGATTGGG CGGCCGTGCG GGAAGATCAA CCGCTAACCG TTGTCGGCAT CGACCGCCCG ACGATTGACC GCGACGTGAA GGCCATCGGC CGGCGCGACT TCGTAGTGAT CGACGGAGCG CCCCAGGCGG CGGACTTGGC TGTGTCCGCG ATCAAGGCAG CCGACTTCGT GCTGATTCCG GTGCAGCCAA GCCCTTACGA CATATGGGCC ACCGCCGACC TGGTGGAGCT GGTTAAGCAG CGCATTGAGG TCACGGATGG AAGGCTACAA GCGGCCTTTG TCGTGTCGCG GGCGATCAAA GGCACGCGCA TCGGCGGTGA GGTTGCCGAG GCGCTGGCCG GGTACGAGCT GCCCATTCTT GAGTCCCGTA TCACGCAGCG CGTGAGCTAC CCAGGCACTG CCGCCGCCGG CACAACCGTT CTTGAATCAG AACCCGAGGG CGACGCTGCC CGCGAGGTCC AGGCGCTGGC CGCTGAAATT AAATCAAAAC TCATTTGAGT TAATGAGGTA AAGAGAAAAT GAGCAAAAGC ACAAACACGC TAAGTGCCGG CCGTCCGAGC GCACGCAGCA GCAAGGCTGC AACGTTGGCC AGCCTGGCAG ACACGCCAGC CATGAAGCGG GTCAACTTTC AGTTGCCGGC GGAGGATCAC ACCAAGCTGA AGATGTACGC GGTACGCCAA GGCAAGACCA TTACCGAGCT GCTATCTGAA TACATCGCGC AGCTACCAGA GTAAATGAGC AAATGAATAA ATGAGTAGAT GAATTTTAGC GGCTAAAGGA GGCGGCATGG AAAATCAAGA ACAACCAGGC ACCGACGCCG TGGAATGCCC CATGTGTGGA GGAACGGGCG GTTGGCCAGG CGTAAGCGGC TGGGTTGTCT GCCGGCCCTG CAATGGCACT GGAACCCCCA AGCCCGAGGA ATCGGCGTGA CGGTCGCAAA CCATCCGGCC CGGTACAAAT CGGCGCGGCG CTGGGTGATG ACCTGGTGGA GAAGTTGAAG GCCGCGCAGG CCGCCCAGCG GCAACGCATC GAGGCAGAAG CACGCCCCGG TGAATCGTGG CAAGCGGCCG CTGATCGAAT CCGCAAAGAA TCCCGGCAAC CGCCGGCAGC CGGTGCGCCG TCGATTAGGA AGCCGCCCAA GGGCGACGAG CAACCAGATT TTTTCGTTCC GATGCTCTAT GACGTGGGCA CCCGCGATAG TCGCAGCATC ATGGACGTGG CCGTTTTCCG TCTGTCGAAG CGTGACCGAC GAGCTGGCGA GGTGATCCGC TACGAGCTTC CAGACGGGCA CGTAGAGGTT TCCGCAGGGC CGGCCGGCAT GGCCAGTGTG TGGGATTACG ACCTGGTACT GATGGCGGTT TCCCATCTAA CCGAATCCAT GAACCGATAC CGGGAAGGGA AGGGAGACAA GCCCGGCCGC GTGTTCCGTC CACACGTTGC GGACGTACTC AAGTTCTGCC GGCGAGCCGA TGGCGGAAAG CAGAAAGACG ACCTGGTAGA AACCTGCATT CGGTTAAACA CCACGCACGT TGCCATGCAG CGTACGAAGA AGGCCAAGAA CGGCCGCCTG GTGACGGTAT CCGAGGGTGA AGCCTTGATT AGCCGCTACA AGATCGTAAA GAGCGAAACC GGGCGGCCGG AGTACATCGA GATCGAGCTA GCTGATTGGA TGTACCGCGA GATCACAGAA GGCAAGAACC CGGACGTGCT GACGGTTCAC CCCGATTACT TTTTGATCGA TCCCGGCATC GGCCGTTTTC TCTACCGCCT GGCACGCCGC GCCGCAGGCA AGGCAGAAGC CAGATGGTTG TTCAAGACGA TCTACGAACG CAGTGGCAGC GCCGGAGAGT TCAAGAAGTT CTGTTTCACC GTGCGCAAGC TGATCGGGTC AAATGACCTG CCGGAGTACG ATTTGAAGGA GGAGGCGGGG CAGGCTGGCC CGATCCTAGT CATGCGCTAC CGCAACCTGA TCGAGGGCGA AGCATCCGCC GGTTCCTAAT GTACGGAGCA GATGCTAGGG CAAATTGCCC TAGCAGGGGA AAAAGGTCGA AAAGGTCTCT TTCCTGTGGA TAGCACGTAC ATTGGGAACC CAAAGCCGTA CATTGGGAAC CGGAACCCGT ACATTGGGAA CCCAAAGCCG TACATTGGGA ACCGGTCACA CATGTAAGTG ACTGATATAA AAGAGAAAAA AGGCGATTTT TCCGCCTAAA ACTCTTTAAA ACTTATTAAA ACTCTTAAAA CCCGCCTGGC CTGTGCATAA CTGTCTGGCC AGCGCACAGC CGAAGAGCTG CAAAAAGCGC CTACCCTTCG GTCGCTGCGC TCCCTACGCC CCGCCGCTTC GCGTCGGCCT ATCGCGGCCG CTGGCCGCTC AAAAATGGCT GGCCTACGGC CAGGCAATCT ACCAGGGCGC GGACAAGCCG CGCCGTCGCC ACTCGACCGC CGGCGCCCAC ATCAAGGCAC CCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC ACGTAGCGAT AGCGGAGTGT ATACTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGCATTCTAG GTACTAAAAC AATTCATCCA GTAAAATATA ATATTTTATT TTCTCCCAAT CAGGCTTGAT CCCCAGTAAG TCAAAAAATA GCTCGACATA CTGTTCTTCC CCGATATCCT CCCTGATCGA CCGGACGCAG AAGGCAATGT CATACCACTT GTCCGCCCTG CCGCTTCTCC CAAGATCAAT AAAGCCACTT ACTTTGCCAT CTTTCACAAA GATGTTGCTG TCTCCCAGGT CGCCGTGGGA AAAGACAAGT TCCTCTTCGG GCTTTTCCGT CTTTAAAAAA TCATACAGCT CGCGCGGATC TTTAAATGGA GTGTCTTCTT CCCAGTTTTC GCAATCCACA TCGGCCAGAT CGTTATTCAG TAAGTAATCC AATTCGGCTA AGCGGCTGTC TAAGCTATTC GTATAGGGAC AATCCGATAT GTCGATGGAG TGAAAGAGCC TGATGCACTC CGCATACAGC TCGATAATCT TTTCAGGGCT TTGTTCATCT TCATACTCTT CCGAGCAAAG GACGCCATCG GCCTCACTCA TGAGCAGATT GCTCCAGCCA TCATGCCGTT CAAAGTGCAG GACCTTTGGA ACAGGCAGCT TTCCTTCCAG CCATAGCATC ATGTCCTTTT CCCGTTCCAC ATCATAGGTG GTCCCTTTAT ACCGGCTGTC CGTCATTTTT AAATATAGGT TTTCATTTTC TCCCACCAGC TTATATACCT TAGCAGGAGA CATTCCTTCC GTATCTTTTA CGCAGCGGTA TTTTTCGATC AGTTTTTTCA ATTCCGGTGA TATTCTCATT TTAGCCATTT ATTATTTCCT TCCTCTTTTC TACAGTATTT AAAGATACCC CAAGAAGCTA ATTATAACAA GACGAACTCC AATTCACTGT TCCTTGCATT CTAAAACCTT AAATACCAGA AAACAGCTTT TTCAAAGTTG TTTTCAAAGT TGGCGTATAA CATAGTATCG ACGGAGCCGA TTTTGAAACC GCGGTGATCA CAGGCAGCAA CGCTCTGTCA TCGTTACAAT CAACATGCTA CCCTCCGCGA GATCATCCGT GTTTCAAACC CGGCAGCTTA GTTGCCGTTC TTCCGAATAG CATCGGTAAC ATGAGCAAAG TCTGCCGCCT TACAACGGCT CTCCCGCTGA CGCCGTCCCG GACTGATGGG CTGCCTGTAT CGAGTGGTGA TTTTGTGCCG AGCTGCCGGT CGGGGAGCTG TTGGCTGGCT GGTGGCAGGA TATATTGTGG TGTAAACAAA TTGACGCTTA GACAACTTAA TAACACATTG CGGACGTTTT TAATGTACTG AATTAACGCC GAATTAATTC GGGGGATCTG GATTTTAGTA CTGGATTTTG GTTTTAGGAA TTAGAAATTT TATTGATAGA AGTATTTTAC AAATACAAAT ACATACTAAG GGTTTCTTAT ATGCTCAACA CATGAGCGAA ACCCTATAGG AACCCTAATT CCCTTATCTG GGAACTACTC ACACATTATT ATGGAGAAAC TCGAGCTTGT CGATCGACAG ATCCGGTCGG CATCTACTCT ATTTCTTTGC CCTCGGACGA GTGCTGGGGC GTCGGTTTCC ACTATCGGCG AGTACTTCTA CACAGCCATC GGTCCAGACG GCCGCGCTTC TGCGGGCGAT TTGTGTACGC CCGACAGTCC CGGCTCCGGA TCGGACGATT GCGTCGCATC GACCCTGCGC CCAAGCTGCA TCATCGAAAT TGCCGTCAAC CAAGCTCTGA TAGAGTTGGT CAAGACCAAT GCGGAGCATA TACGCCCGGA GTCGTGGCGA TCCTGCAAGC TCCGGATGCC TCCGCTCGAA GTAGCGCGTC TGCTGCTCCA TACAAGCCAA CCACGGCCTC CAGAAGAAGA TGTTGGCGAC CTCGTATTGG GAATCCCCGA ACATCGCCTC GCTCCAGTCA ATGACCGCTG TTATGCGGCC ATTGTCCGTC AGGACATTGT TGGAGCCGAA ATCCGCGTGC ACGAGGTGCC GGACTTCGGG GCAGTCCTCG GCCCAAAGCA TCAGCTCATC GAGAGCCTGC GCGACGGACG CACTGACGGT GTCGTCCATC ACAGTTTGCC AGTGATACAC ATGGGGATCA GCAATCGCGC ATATGAAATC ACGCCATGTA GTGTATTGAC CGATTCCTTG CGGTCCGAAT GGGCCGAACC CGCTCGTCTG GCTAAGATCG GCCGCAGCGA TCGCATCCAT AGCCTCCGCG ACCGGTTGTA GAACAGCGGG CAGTTCGGTT TCAGGCAGGT CTTGCAACGT GACACCCTGT GCACGGCGGG AGATGCAATA GGTCAGGCTC TCGCTAAACT CCCCAATGTC AAGCACTTCC GGAATCGGGA GCGCGGCCGA TGCAAAGTGC CGATAAACAT AACGATCTTT GTAGAAACCA TCGGCGCAGC TATTTACCCG CAGGACATAT CCACGCCCTC CTACATCGAA GCTGAAAGCA CGAGATTCTT CGCCCTCCGA GAGCTGCATC AGGTCGGAGA CGCTGTCGAA CTTTTCGATC AGAAACTTCT CGACAGACGT CGCGGTGAGT TCAGGCTTTT TCATATCTCA TTGCCCCCCG GATCTGCGAA AGCTCGAGAG AGATAGATTT GTAGAGAGAG ACTGGTGATT TCAGCGTGTC CTCTCCAAAT GAAATGAACT TCCTTATATA GAGGAAGGTC TTGCGAAGGA TAGTGGGATT GTGCGTCATC CCTTACGTCA GTGGAGATAT CACATCAATC CACTTGCTTT GAAGACGTGG TTGGAACGTC TTCTTTTTCC ACGATGCTCC TCGTGGGTGG GGGTCCATCT TTGGGACCAC TGTCGGCAGA GGCATCTTGA ACGATAGCCT TTCCTTTATC GCAATGATGG CATTTGTAGG TGCCACCTTC CTTTTCTACT GTCCTTTTGA TGAAGTGACA GATAGCTGGG CAATGGAATC CGAGGAGGTT TCCCGATATT ACCCTTTGTT GAAAAGTCTC AATAGCCCTT TGGTCTTCTG AGACTGTATC TTTGATATTC TTGGAGTAGA CGAGAGTGTC GTGCTCCACC ATGTTATCAC ATCAATCCAC TTGCTTTGAA GACGTGGTTG GAACGTCTTC TTTTTCCACG ATGCTCCTCG TGGGTGGGGG TCCATCTTTG GGACCACTGT CGGCAGAGGC ATCTTGAACG ATAGCCTTTC CTTTATCGCA ATGATGGCAT TTGTAGGTGC CACCTTCCTT TTCTACTGTC CTTTTGATGA AGTGACAGAT AGCTGGGCAA TGGAATCCGA GGAGGTTTCC CGATATTACC CTTTGTTGAA AAGTCTCAAT AGCCCTTTGG TCTTCTGAGA CTGTATCTTT GATATTCTTG GAGTAGACGA GAGTGTCGTG CTCCACCATG TTGGCAAGCT GCTCTAGCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT AC
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