pMA5
$128.00
In stock
SKU
PL81506
100μg liquid plasmid(Instantly):$149
| name | pMA5 | another name | apMA5 |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Kan | Fluorescent labeling | |
| Promoter | HpaII | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CATATGAGTT ATGCAGTTTG TAGAATGCAA AAAGTGAAAT CAGGGGGATC CTCTAGAGTC GAGCTCAAGC TAGCTTGGTA CGTACCAGAT CTGAGATCAC GCGTTCTAGA GGTCGAAATT CACCTCGAAA GCAAGCTGAT AAACCGATAC AATTAAAGGC TCCTTTTGGA GCCTTTTTTT TTGGAGATTT TCAACGTGAA AAAATTATTA TTCGCAATTC CAAGCTAATT CACCTCGAAA GCAAGCTGAT AAACCGATAC AATTAAAGGC TCCTTTTGGA GCCTTTTTTT TTGGAGATTT TCAACGTGAA AAAATTATTA TTCGCAATTC CAAGCTCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCAGATCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA ATTTGATCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGCGGTA ATACGGTTAT CCCAGAATCC GGGGATAACG CAGGAAAGAC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG AAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTAAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC AGGGCTTCCC GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG TGATCTTCCG TCACAGGTAT TTATTCGAAG ACGAAAGGGC ATCGCGCGCG GGGAATTCCC GGGAGAGCTC GATATCGCAT GCGGTACCTC TAGAAGAAGC TTGGAGACAA GGTAAAGGAT AAAACAGCAC AATTCCAAGA AAAACACGAT TTAGAACCTA AAAAGAACGA ATTTGAACTA ACTCATAACC GAGAGGTAAA AAAAGAACGA AGTCGAGATC AGGGAATGAG TTTATAAAAT AAAAAAAGCA CCTGAAAAGG TGTCTTTTTT TGATGGTTTT GAACTTGTTC TTTCTTATCT TGATACATAT AGAAATAACG TCATTTTTAT TTTAGTTGCT GAAAGGTGCG TTGAAGTGTT GGTATGTATG TGTTTTAAAG TATTGAAAAC CCTTAAAATT GGTTGCACAG AAAAACCCCA TCTGTTAAAG TTATAAGTGA CTAAACAAAT AACTAAATAG ATGGGGGTTT CTTTTAATAT TATGTGTCCT AATAGTAGCA TTTATTCAGA TGAAAAATCA AGGGTTTTAG TGGACAAGAC AAAAAGTGGA AAAGTGAGAC CATGGAGAGA AAAGAAAATC GCTAATGTTG ATTACTTTGA ACTTCTGCAT ATTCTTGAAT TTAAAAAGGC TGAAAGAGTA AAAGATTGTG CTGAAATATT AGAGTATAAA CAAAATCGTG AAACAGGCGA AAGAAAGTTG TATCGAGTGT GGTTTTGTAA ATCCAGGCTT TGTCCAATGT GCAACTGGAG GAGAGCAATG AAACATGGCA TTCAGTCACA AAAGGTTGTT GCTGAAGTTA TTAAACAAAA GCCAACAGTT CGTTGGTTGT TTCTCACATT AACAGTTAAA AATGTTTATG ATGGCGAAGA ATTAAATAAG AGTTTGTCAG ATATGGCTCA AGGATTTCGC CGAATGATGC AATATAAAAA AATTAATAAA AATCTTGTTG GTTTTATGCG TGCAACGGAA GTGACAATAA ATAATAAAGA TAATTCTTAT AATCAGCACA TGCATGTATT GGTATGTGTG GAACCAACTT ATTTTAAGAA TACAGAAAAC TACGTGAATC AAAAACAATG GATTCAATTT TGGAAAAAGG CAATGAAATT AGACTATGAT CCAAATGTAA AAGTTCAAAT GATTCGACCG AAAAATAAAT ATAAATCGGA TATACAATCG GCAATTGACG AAACTGCAAA ATATCCTGTA AAGGATACGG ATTTTATGAC CGATGATGAA GAAAAGAATT TGAAACGTTT GTCTGATTTG GAGGAAGGTT TACACCGTAA AAGGTTAATC TCCTATGGTG GTTTGTTAAA AGAAATACAT AAAAAATTAA ACCTTGATGA CACAGAAGAA GGCGATTTGA TTCATACAGA TGATGACGAA AAAGCCGATG AAGATGGATT TTCTATTATT GCAATGTGGA ATTGGGAACG GAAAAATTAT TTTATTAAAG AGTAGTTCAA CAAACGGGCC AGTTTGTTGA AGATTAGATG CTATAATTGT TATTAAAAGG ATTGAAGGAT GCTTAGGAAG ACGAGTTATT AATAGCTGAA TAAGAACGGT GCTCTCCAAA TATTCTTATT TAGAAAAGCA AATCTAAAAT TATCTGAAAA GGGAATGAGA ATAGTGAATG GACCAATAAT AATGACTAGA GAAGAAAGAA TGAAGATTGT TCATGAAATT AAGGAACGAA TATTGGATAA ATATGGGGAT GATGTTAAGG CTATTGGTGT TTATGGCTCT CTTGGTCGTC AGACTGATGG GCCCTATTCG GATATTGAGA TGATGTGTGT CATGTCAACA GAGGAAGCAG AGTTCAGCCA TGAATGGACA ACCGGTGAGT GGAAGGTGGA AGTGAATTTT GATAGCGAAG AGATTCTACT AGATTATGCA TCTCAGGTGG AATCAGATTG GCCGCTTACA CATGGTCAAT TTTTCTCTAT TTTGCCGATT TATGATTCAG GTGGATACTT AGAGAAAGTG TATCAAACTG CTAAATCGGT AGAAGCCCAA ACGTTCCACG ATGCGATTTG TGCCCTTATC GTAGAAGAGC TGTTTGAATA TGCAGGCAAA TGGCGTAATA TTCGTGTGCA AGGACCGACA ACATTTCTAC CATCCTTGAC TGTACAGGTA GCAATGGCAG GTGCCATGTT GATTGGTCTG CATCATCGCA TCTGTTATAC GACGAGCGCT TCGGTCTTAA CTGAAGCAGT TAAGCAATCA GATCTTCCTT CAGGTTATGA CCATCTGTGC CAGTTCGTAA TGTCTGGTCA ACTTTCCGAC TCTGAGAAAC TTCTGGAATC GCTAGAGAAT TTCTGGAATG GGATTCAGGA GTGGACAGAA CGACACGGAT ATATAGTGGA TGTGTCAAAA CGCATACCAT TTTGAACGAT GACCTCTAAT AATTGTTAAT CATGTTGGTT ACGTATTTAT TAACTTCTCC TAGTATTAGT AATTATCATG GCTGTCATGG CGCATTAACG GAATAAAGGG TGTGCTTAAA TCGGGCCATT TTGCGTAATA AGAAAAAGGA TTAATTATGA GCGAATTGAA TTAATAATAA GGTAATAGAT TTACATTAGA AAATGAAAGG GGATTTTATG CGTGAGAATG TTACAGTCTA TCCCGGCATT GCCAGTCGGG GATATTAAAA AGAGTATAGG TTTTTATTGC GATAAACTAG GTTTCACTTT GGTTCACCAT GAAGATGGAT TCGCAGTTCT AATGTGTAAT GAGGTTCGGA TTCATCTATG GGAGGCAAGT GATGAAGGCT GGCGCTCTCG TAGTAATGAT TCACCGGTTT GTACAGGTGC GGAGTCGTTT ATTGCTGGTA CTGCTAGTTG CCGCATTGAA GTAGAGGGAA TTGATGAATT ATATCAACAT ATTAAGCCTT TGGGCATTTT GCACCCCAAT ACATCATTAA AAGATCAGTG GTGGGATGAA CGAGACTTTG CAGTAATTGA TCCCGACAAC AATTTGATTA GCTTTTTTCA ACAAATAAAA AGCTAAAATC TATTATTAAT CTGTTCAGCA ATCGGGCGCG ATTGCTGAAT AAAAGATACG AGAGACCTCT CTTGTATCTT TTTTATTTTG AGTGGTTTTG TCCGTTACAC TAGAAAACCG AAAGACAATA AAAATTTTAT TCTTGCTGAG TCTGGCTTTC GGTAAGCTAG ACAAAACGGA CAAAATAAAA ATTGGCAAGG GTTTAAAGGT GGAGATTTTT TGAGTGATCT TCTCAAAAAA TACTACCTGT CCCTTGCTGA TTTTTAAACG AGCACGAGAG CAAAACCCCC CTTTGCTGAG GTGGCAGAGG GCAGGTTTTT TTGTTTCTTT TTTCTCGTAA AAAAAAGAAA GGTCTTAAAG GTTTTATGGT TTTGGTCGGC ACTGCCGACA GCCTCGCAGA GCACACACTT TATGAATATA AAGTATAGTG TGTTATACTT TACTTGGAAG TGGTTGCCGG AAAGAGCGAA AATGCCTCAC ATTTGTGCCA CCTAAAAAGG AGCGATTTA
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
