pMA0911
$128.00
In stock
SKU
PL81503
100μg liquid plasmid(Instantly):$149
| name | pMA0911 | another name | apMA0911 |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression,Secretion expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Hpall | Replicon | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CATATCCTCA TATGGTCAGC ATCCGCCGCA GCTTCGAAGC GTATGTCGAT GACATGAATA TCATTACTGT TCTGATTCCT GCTGAACAAA AGGAAATCAT GACACCGCCG GAATTCGTCG ACAAGCTTGG TACCAGATCT GATATCCCTG CAGGGATCCT CTAGAGTCGA GCTCAAGCTA GCTTGGTACG TACCAGATCT GAGATCCCGG GTTCTAGAGG TCGAAATTCA CCTCGAAAGC AAGCTGATAA ACCGATACAA TTAAAGGCTC CTTTTGGAGC CTTTTTTTTT GGAGATTTTC AACGTGAAAA AATTATTATT CGCAATTCCA AGCTAATTCA CCTCGAAAGC AAGCTGATAA ACCGATACAA TTAAAGGCTC CTTTTGGAGC CTTTTTTTTT GGAGATTTTC AACGTGAAAA AATTATTATT CGCAATTCCA AGCTCTGCCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CAGATCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAC TGCAATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT GCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAAGTACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGAAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTAAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCGATCAAC GTCTCATTTT CGCCAAAAGT TGGCCCAGGG CTTCCCGGTA TCAACAGGGA CACCAGGATT TATTTATTCT GCGAAGTGAT CTTCCGTCAC AGGTATTTAT TCGAAGACGA AAGGGCATCG CGCGCGGGAA ATTCCCGGGA GAGCTCGATA TCGCATGCGG TACCTCTAGA AGAAGCTTGG AGACAAGGTA AAGGATAAAA CAGCACAATT CCAAGAAAAA CACGATTTAG AACCTAAAAA GAACGAATTT GAACTAACTC ATAACCGAGA GGTAAAAAAA GAACGAAGTC GAGATCAGGG AATGAGTTTA TAAAATAAAA AAAGCACCTG AAAAGGTGTC TTTTTTTGAT GGTTTTGAAC TTGTTCTTTC TTATCTTGAT ACATATAGAA ATAACGTCAT TTTTATTTTA GTTGCTGAAA GGTGCGTTGA AGTGTTGGTA TGTATGTGTT TTAAAGTATT GAAAACCCTT AAAATTGGTT GCACAGAAAA ACCCCATCTG TTAAAGTTAT AAGTGACTAA ACAAATAACT AAATAGATGG GGGTTTCTTT TAATATTATG TGTCCTAATA GTAGCATTTA TTCAGATGAA AAATCAAGGG TTTTAGTGGA CAAGACAAAA AGTGGAAAAG TGAGACCATG GAGAGAAAAG AAAATCGCTA ATGTTGATTA CTTTGAACTT CTGCATATTC TTGAATTTAA AAAGGCTGAA AGAGTAAAAG ATTGTGCTGA AATATTAGAG TATAAACAAA ATCGTGAAAC AGGCGAAAGA AAGTTGTATC GAGTGTGGTT TTGTAAATCC AGGCTTTGTC CAATGTGCAA CTGGAGGAGA GCAATGAAAC ATGGCATTCA GTCACAAAAG GTTGTTGCTG AAGTTATTAA ACAAAAGCCA ACAGTTCGTT GGTTGTTTCT CACATTAACA GTTAAAAATG TTTATGATGG CGAAGAATTA AATAAGAGTT TGTCAGATAT GGCTCAAGGA TTTCGCCGAA TGATGCAATA TAAAAAAATT AATAAAAATC TTGTTGGTTT TATGCGTGCA ACGGAAGTGA CAATAAATAA TAAAGATAAT TCTTATAATC AGCACATGCA TGTATTGGTA TGTGTGGAAC CAACTTATTT TAAGAATACA GAAAACTACG TGAATCAAAA ACAATGGATT CAATTTTGGA AAAAGGCAAT GAAATTAGAC TATGATCCAA ATGTAAAAGT TCAAATGATT CGACCGAAAA ATAAATATAA ATCGGATATA CAATCGGCAA TTGACGAAAC TGCAAAATAT CCTGTAAAGG ATACGGATTT TATGACCGAT GATGAAGAAA AGAATTTGAA ACGTTTGTCT GATTTGGAGG AAGGTTTACA CCGTAAAAGG TTAATCTCCT ATGGTGGTTT GTTAAAAGAA ATACATAAAA AATTAAACCT TGATGACACA GAAGAAGGCG ATTTGATTCA TACAGATGAT GACGAAAAAG CCGATGAAGA TGGATTTTCT ATTATTGCAA TGTGGAATTG GGAACGGAAA AATTATTTTA TTAAAGAGTA GTTCAACAAA CGGGCCAGTT TGTTGAAGAT TAGATGCTAT AATTGTTATT AAAAGGATTG AAGGATGCTT AGGAAGACGA GTTATTAATA GCTGAATAAG AACGGTGCTC TCCAAATATT CTTATTTAGA AAAGCAAATC TAAAATTATC TGAAAAGGGA ATGAGAATAG TGAATGGACC AATAATAATG ACTAGAGAAG AAAGAATGAA GATTGTTCAT GAAATTAAGG AACGAATATT GGATAAATAT GGGGATGATG TTAAGGCTAT TGGTGTTTAT GGCTCTCTTG GTCGTCAGAC TGATGGGCCC TATTCGGATA TTGAGATGAT GTGTGTCATG TCAACAGAGG AAGCAGAGTT CAGCCATGAA TGGACAACCG GTGAGTGGAA GGTGGAAGTG AATTTTGATA GCGAAGAGAT TCTACTAGAT TATGCATCTC AGGTGGAATC AGATTGGCCG CTTACACATG GTCAATTTTT CTCTATTTTG CCGATTTATG ATTCAGGTGG ATACTTAGAG AAAGTGTATC AAACTGCTAA ATCGGTAGAA GCCCAAACGT TCCACGATGC GATTTGTGCC CTTATCGTAG AAGAGCTGTT TGAATATGCA GGCAAATGGC GTAATATTCG TGTGCAAGGA CCGACAACAT TTCTACCATC CTTGACTGTA CAGGTAGCAA TGGCAGGTGC CATGTTGATT GGTCTGCATC ATCGCATCTG TTATACGACG AGCGCTTCGG TCTTAACTGA AGCAGTTAAG CAATCAGATC TTCCTTCAGG TTATGACCAT CTGTGCCAGT TCGTAATGTC TGGTCAACTT TCCGACTCTG AGAAACTTCT GGAATCGCTA GAGAATTTCT GGAATGGGAT TCAGGAGTGG ACAGAACGAC ACGGATATAT AGTGGATGTG TCAAAACGCA TACCATTTTG AACGATGACC TCTAATAATT GTTAATCATG TTGGTTACGT ATTTATTAAC TTCTCCTAGT ATTAGTAATT ATCATGGCTG TCATGGCGCA TTAACGGAAT AAAGGGTGTG CTTAAATCGG GCCATTTTGC GTAATAAGAA AAAGGATTAA TTATGAGCGA ATTGAATTAA TAATAAGGTA ATAGATTTAC ATTAGAAAAT GAAAGGGGAT TTTATGCGTG AGAATGTTAC AGTCTATCCC GGCATTGCCA GTCGGGGATA TTAAAAAGAG TATAGGTTTT TATTGCGATA AACTAGGTTT CACTTTGGTT CACCATGAAG ATGGATTCGC AGTTCTAATG TGTAATGAGG TTCGGATTCA TCTATGGGAG GCAAGTGATG AAGGCTGGCG CTCTCGTAGT AATGATTCAC CGGTTTGTAC AGGTGCGGAG TCGTTTATTG CTGGTACTGC TAGTTGCCGC ATTGAAGTAG AGGGAATTGA TGAATTATAT CAACATATTA AGCCTTTGGG CATTTTGCAC CCCAATACAT CATTAAAAGA TCAGTGGTGG GATGAACGAG ACTTTGCAGT AATTGATCCC GACAACAATT TGATTAGCTT TTTTCAACAA ATAAAAAGCT AAAATCTATT ATTAATCTGT TCAGCAATCG GGCGCGATTG CTGAATAAAA GATACGAGAG ACCTCTCTTG TATCTTTTTT ATTTTGAGTG GTTTTGTCCG TTACACTAGA AAACCGAAAG ACAATAAAAA TTTTATTCTT GCTGAGTCTG GCTTTCGGTA AGCTAGACAA AACGGACAAA ATAAAAATTG GCAAGGGTTT AAAGGTGGAG ATTTTTTGAG TGATCTTCTC AAAAAATACT ACCTGTCCCT TGCTGATTTT TAAACGAGCA CGAGAGCAAA ACCCCCCTTT GCTGAGGTGG CAGAGGGCAG GTTTTTTTGT TTCTTTTTTC TCGTAAAAAA AAGAAAGGTC TTAAAGGTTT TATGGTTTTG GTCGGCACTG CCGACAGCCT CGCAGAGCAC ACACTTTATG AATATAAAGT ATAGTGTGTT ATACTTTACT TGGAAGTGGT TGCCGGAAAG AGCGAAAATG CCTCACATTT GTGCCACCTA AAAAGGAGCG ATTTA
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