pIEFBPR
$128.00
In stock
SKU
PL81500
100μg liquid plasmid(Instantly):$149
| name | pIEFBPR | another name | apIEFBPR |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGGTACCGG GCCCCCCCTC GAGTTAACCT GCAGGCGCGC CTTTGATGGG TATCGGTATT GCTACTGGGG AAAATCGCGC GGCAGAGGCA GCAAAAAAAG CAATTTCCAG CCCGCTTCTT GAAGCGGCCA TTGACGGTGC GCAAGGCGTC CTCATGAACA TCACTGGAGG AACAAACCTC AGCCTATATG AGGTTCAGGA AGCAGCAGAC ATTGTCGCTT CGGCGTCTGA TCAAGACGTA AACATGATTT TCGGTTCTGT TATTAATGAA AATCTAAAAG ATGAGATTGT GGTGACAGTG ATTGCAACCG GCTTTATCGA ACAAGAGAAG GACGTGACGA AGCCTCAGCG CCCAAGCTTA AATCAAAGCA TCAAAACACA CAATCAAAGT GTTCCGAAGC GTGACGCAAA ACGTGAGGAA CCTCAGCAGC AGAACACAGT AAGCCGTCAT ACTTCACAGC CGGCTGATGA TACGCTTGAC ATCCCGACAT TCTTAAGAAA CCGTAATAAA CGCGGCTAAT GTAAAGGACA AAATCGTTTT CGATTTTGTC TTTTTTGTTT TTCTCTTCAC ACTTCCTTCT TATAAAGTCT TTTTCCCTAT TGCTTTCCTT CGCTTAGTAA CAAAACAGAT AATTAGACCC ATTTATTTTT GTGACATTTT TATCATTTTC ATATATATGG AAATTGAATG ACATGAAACG ACAATATCTG TAATTCAGAT TGTCTACAGT TAATATACAG CGATGTTCTG ACAAACCATT CATTATTAAA AGGAGGGACG ACACTTTTTT TAAAAAGCAT GTTGAAAAAG GGGGATGAAA ATGAGGAAAA AAACGAAAAA CAGACTCATC AGCTCTGTTT TAAGTACAGT TGTCATCAGT TCACTGCTGT TTCCGGGAGC AGCCGGGGCA AGCAGTAAAG TCACCTCACC TTCTGTTAAA AAGGAGCTTC AATCTGCGGA ATCCATTCAA AACAAGATTT CGAGTTCATT AAAGAAAAGC TTTAAAAAGA AAGAAAAAAC GACTTTTCTG ATTAAATTTA AAGATCTATC GATGCTAGCC ATGGTCGACG CGGCCGCAAA ATTGAAAAAA TGGTGGAAAC ACTTTTTTCA ATTTTTTTGT TTTATTATTT AATATTTGGG AAATATTCAT TCTAATTGGT AATCAGATTT TAGAAAACAA TAAACCCTTG CATAGGGGGA TCTCCGGAGT TTAAACCGCG GATCCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCCAGGGTGG TTTTTCTTTT CACCAGTGAG ACGGGCAACA GCTGATTGCC CTTCACCGCC TGGCCCTGAG AGAGTTGCAG CAAGCGGTCC ACGCTGGTTT GCCCCAGCAG GCGAAAATCC TGTTTGATGG TGGTTGACGG CGGGATATAA CATGAGCTGT CTTCGGTATC GTCGTATCCC ACTACCGAGA TATCCGCACC AACGCGCAGC CCGGACTCGG TAATGGCGCG CATTGCGCCC AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA CGATGCCCTC ATTCAGCATT TGCATGGTTT GTTGAAAACC GGACATGGCA CTCCAGTCGC CTTCCCGTTC CGCTATCGGC TGAATTTGAT TGCGAGTGAG ATATTTATGC CAGCCAGCCA GACGCAGACG CGCCGAGACA GAACTTAATG GGCCCGCTAA CAGCGCGATT TGCTGGTGAC CCAATGCGAC CAGATGCTCC ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC TGTTGATGGG TGTCTGGTCA GAGACATCAA GAAATAACGC CGGAACATTA GTGCAGGCAG CTTCCACAGC AATGGCATCC TGGTCATCCA GCGGATAGTT AATGATCAGC CCACTGACGC GTTGCGCGAG AAGATTGTGC ACCGCCGCTT TACAGGCTTC GACGCCGCTT CGTTCTACCA TCGACACCAC CACGCTGGCA CCCAGTTGAT CGGCGCGAGA TTTAATCGCC GCGACAATTT GCGACGGCGC GTGCAGGGCC AGACTGGAGG TGGCAACGCC AATCAGCAAC GACTGTTTGC CCGCCAGTTG TTGTGCCACG CGGTTGGGAA TGTAATTCAG CTCCGCCATC GCCGCTTCCA CTTTTTCCCG CGTTTTCGCA GAAACGTGGC TGGCCTGGTT CACCACGCGG GAAACGGTCT GATAAGAGAC ACCGGCATAC TCTGCGACAT CGTATAACGT TACTGGTTTC ATCAAAATCG TCTCCCTCCG TTTGAATATT TGATTGATCG TAACCAGATG AAGCACTCTT TCCACTATCC CTACAGTGTT ATGGCTTGAA CAATCACGAA ACAATAATTG GTACGTACGA TCTTTCAGCC GACTCAAACA TCAAATCTTA CAAATGTAGT CTTTGAAAGT ATTACATATG TAAGATTTAA ATGCAACCGT TTTTTCGGAA GGAAATGATG ACCTCGTTTC CACCGGAATT AGCTTGCATG CCTACCCAAT CAGTACGTTA ATTTTGGCTT TAATGAGTTG TAATTCCTCT GGGGCAACTG TTCCTTTCTT CGTTGCTCCT CTTGCCCGCC AGGCGATACT TTTTACCTGA TCAGCTAACG CTACGCCATC ACGTTCCTGA CCGGATAAAA CAACTTCGAA CGGATATCCT TTTGATTGCG TTGTACAAGG AACACACAGA CACATACCTG TTTTGTTGTT GTACATGAAA GGACTCAGGA CAACAGCTGG ACGATGTCCA GCTTGCTCGC TACCTTTTGT CGGGTCAAAA TCAACCCAAA TCAGATCGCC CATATCGGGT ACGTATCGGC TTACCATACC TGCTTCCTCC TTAAGCTTAA TTGTTATCCG CTCACAATTC CACACATTAT GCCACACCTT GTAGATAAAG TCAACAACTT TTTGCAAAAT TTTTCAGGAA TTTTAGCAGA GGTTGTTCTG GATGTAGAAC AAAACATCTT TCCGCTCTTG TGCTGTTAGG ATATCTTTCT TGGAAGCTAG GTAGGCAAGG GCTACCTCAA ATAAATCTTC TTCAGGGTGC GCTATTTTTA AGGTGCCTAG TGAGGTCTTG ACCACTTCAC CCATAATTTC AGTGCCGAAT AGTCTGGACT GGGCTGTGTA GAATTCCTGC AGCCCTGGCG AATGGCGATT TTCGTTCGTG AATACATGTT ATAATAACTA TAACTAATAA CGTAACGTGA CTGGCAAGAG ATATTTTTAA AACAATGAAT AGGTTTACAC TTACTTTAGT TTTATGGAAA TGAAAGATCA TATCATATAT AATCTAGAAT AAAATTAACT AAAATAATTA TTATCTAGAT AAAAAATTTA GAAGCCAATG AAATCTATAA ATAAACTAAA TTAAGTTTAT TTAATTAACA ACTATGGATA TAAAATAGGT ACTAATCAAA ATAGTGAGGA GGATATATTT GAATACATAC GAACAAATTA ATAAAGTGAA AAAAATACTT CGGAAACATT TAAAAAATAA CCTTATTGGT ACTTACATGT TTGGATCAGG AGTTGAGAGT GGACTAAAAC CAAATAGTGA TCTTGACTTT TTAGTCGTCG TATCTGAACC ATTGACAGAT CAAAGTAAAG AAATACTTAT ACAAAAAATT AGACCTATTT CAAAAAAAAT AGGAGATAAA AGCAACTTAC GATATATTGA ATTAACAATT ATTATTCAGC AAGAAATGGT ACCGTGGAAT CATCCTCCCA AACAAGAATT TATTTATGGA GAATGGTTAC AAGAGCTTTA TGAACAAGGA TACATTCCTC AGAAGGAATT AAATTCAGAT TTAACCATAA TGCTTTACCA AGCAAAACGA AAAAATAAAA GAATATACGG AAATTATGAC TTAGAGGAAT TACTACCTGA TATTCCATTT TCTGATGTGA GAAGAGCCAT TATGGATTCG TCAGAGGAAT TAATAGATAA TTATCAGGAT GATGAAACCA ACTCTATATT AACTTTATGC CGTATGATTT TAACTATGGA CACGGGTAAA ATCATACCAA AAGATATTGC GGGAAATGCA GTGGCTGAAT CTTCTCCATT AGAACATAGG GAGAGAATTT TGTTAGCAGT TCGTAGTTAT CTTGGAGAGA ATATTGAATG GACTAATGAA AATGTAAATT TAACTATAAA CTATTTAAAT AACAGATTAA AAAAATTATA AAAAAATTGA AAAAATGGTG GAAACACTTT TTTCAATTTT TTTGTTTTAT TATTTAATAT TTGGGAAATA TTCATTCTAA TTGGTAATCA GATTTTAGAA AACAATAAAC CCTTGCATAG GGGGATCTCG ACATGGATGA GCGATGATGA TATCCGTTTA GGCTGGGCGG TGATAGCTTC TCGTTCAGGC AGTACGCCTC TTTTCTTTTC CAGACCTGAG GGAGGCGGAA ATGGTGTGAG GTTCCCGGGC CGGCCAATTG ATGCATCAGT TCAAAAGGGA TTATGGTCGA TAAAGATTTT TATCTCGTAT ATATCCAGTC GAAACCTGAT CCGTATTCAC CTGGATTGGC AATGGATGAA ACCGGCCAGA ATTCCGGCCG CAACTGGCAG TATATAGATG GAAAGTGGCA GCCAGGTGAC AAAGCGGATG GCAACTATAT GATTCGCGCA TTAGTTGATT ATGAAGCTGC TGTACCTGAG ATTACTTCAC CGACAGACAA ATCATACACA AATAAGGATA GCGTCACTGT AAAAGGAAAC GCTTCTCCTG GCACAACGGT ACACATTTAT AATGGAGAGA AAGAAGCAGG AGAAACGAAA GCTGCTGCGG ATGGCACGTT CCATGCAGGC ATCATACTCA ACAAGGGTGA AAATGAGCTG ACGGCAACTG CATCAACTGA CAACGGAACA ACAGATGCCT CCAGCCCAAT CACGGTCACG CTTGATCAAG AAAAGCCTGA ATTAACACTG GACAATCCAA AGGATGGCGG GAAAACAAAT AAAGAAACGC TGACTGTCAA AGGGGCTGTA TCCGATGACA ATCTGAAAGA CGTCAAGGTG AATGGCAAAA AAGCAACAGT AGCTGATGGT TCATACTCAG CCCGTATTCT TTTGGAAAAT GGAAGAAATG AAATCAAGGT AATTGCTACA GACTTGGCAG GCAACAAAAC GACGAAAAAG ACAGTCATTG ATGTGAACTT TGACAAGCCT GTCATTTCCG GCTTAATTCC GGGAGAGGAT AAAAACTTAA AAGCCGGTGA ATCTGTGAAA ATCGCTTTCT CAAGCGCTGA GGATTTAGAT GCAACGTTTA CCATTCGTAT GCCGCTGACC AATGCAAGAG CGAGTGTGCA AAATGCCACC GAACTCCCGT TAAGAGAAAT CTCTCCGGGG AGATATGAAG GCTATTGGAC TGCCACTTCT TCTATTAAAG CAAAAGGAGC AAAAGTAGAA GTGATCGTCC GAGATGATTA TGGAAATGAA ACAAGAAAAA CTGCGAATGG AAAACTTAAT ATGAACACAG AAAATACCGG TCCTAGGGCG ATCGCACTAG TCGATAACCA GAAGCAATAA AAAATCAAAT CGGATTTCAC TATATAATCT CACTTTATCT AAGATGAATC CGATGGAAGC ATCCTGTTTT CTCTCAATTT TTTTATCTAA AACCCAGCGT TCGATGCTTC TTTGAGCGAA CGATCAAAAA TAAGTGCCTT CCCATCAAAA AAATATTCTC AACATAAAAA ACTTTGTGTA ATACTTGTAA CGCTACATGG AGATTAACTC AATCTAGAGG GTATTAATAA TGATCCACAG TAGCGTAAAG CGTTGGGGAA ATTCACCGGC GGTGCGGATT CCGGCTACGT TAATGCAGGC GCTCAATCTG AATATTGATG ATGAAGTGAA GATTGACCTG GTGGATGGCA AATTAATTAT TGAGCCAGTG CGTAAAGAGC CCGTATTTAC GCTTGCTGAA CTGGTCAACG ACATCACGCC GGAAAACCTC CACGAGAATA TCGACTGGGG AGAGCCGAAA GATAAGGAAG TCTGGTAAGA GCTCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTGCGCG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCAC
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